Patents by Inventor John Bridgham

John Bridgham has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Publication number: 20190338351
    Abstract: According to various embodiments, a method is provided that comprises washing an array of DNA-coated beads on a substrate, with a wash solution to remove stacked beads from the substrate. The wash solution can include inert solid beads in a carrier. The DNA-coated beads can have an average diameter and the solid beads in the wash solution can have an average diameter that is at least twice the diameter of the DNA-coated beads. The washing can form dislodged DNA-coated beads and a monolayer of DNA-coated beads. In some embodiments, first beads for forming an array are contacted with a poly(ethylene glycol) (PEG) solution comprising a PEG having a molecular weight of about 350 Da or less. In some embodiments, slides for forming bead arrays are provided as are systems for imaging the same.
    Type: Application
    Filed: July 15, 2019
    Publication date: November 7, 2019
    Inventors: George A. FRY, Christina E. INMAN, John BRIDGHAM, Timothy HUNKAPILLER, Charles S. VANN
  • Publication number: 20190233887
    Abstract: Various embodiments of a low-volume sequencing system are provided herein. The system can include a low-volume flowcell having at least one reaction chamber of a defined volume (e.g., less than about 100 ?l). The system can also include an automated reagent delivery mechanism configured to reversibly couple with the inlet port corresponding to a target reaction chamber thereby placing allowing for reagent to be accurately moved from a storage container to the reaction chamber with minimal reagent waste. The flowcells can include a plurality of reaction chambers (e.g., 6) thereby allowing for parallel analysis of multiple samples. Various methods of analyzing a biomolecule are also provided herein.
    Type: Application
    Filed: December 19, 2018
    Publication date: August 1, 2019
    Inventors: Jon A. HOSHIZAKI, Joon Mo YANG, Maryam SHARIATI, David M. COX, Kirk M. HIRANO, John BRIDGHAM, George Stefan GOLDA, Sam Lee WOO
  • Patent number: 10351907
    Abstract: According to various embodiments, a method is provided that comprises washing an array of DNA-coated beads on a substrate, with a wash solution to remove stacked beads from the substrate. The wash solution can include inert solid beads in a carrier. The DNA-coated beads can have an average diameter and the solid beads in the wash solution can have an average diameter that is at least twice the diameter of the DNA-coated beads. The washing can form dislodged DNA-coated beads and a monolayer of DNA-coated beads. In some embodiments, first beads for forming an array are contacted with a poly(ethylene glycol) (PEG) solution comprising a PEG having a molecular weight of about 350 Da or less. In some embodiments, slides for forming bead arrays are provided as are systems for imaging the same.
    Type: Grant
    Filed: October 16, 2014
    Date of Patent: July 16, 2019
    Assignee: LIFE TECHNOLOGIES CORPORATION
    Inventors: George A. Fry, Christina E. Inman, John Bridgham, Timothy Hunkapiller, Charles S. Vann
  • Patent number: 9273354
    Abstract: An apparatus and system are provided for simultaneously analyzing a plurality of analytes anchored to microparticles. Microparticles each having a uniform population of a single kind of analyte attached are disposed as a substantially immobilized planar array inside of a flow chamber where steps of an analytical process are carried out by delivering a sequence of processing reagents to the microparticles by a fluidic system under microprocessor control. In response to such process steps, an optical signal is generated at the surface of each microparticle which is characteristic of the interaction between the analyte carried by the microparticle and the delivered processing reagent. The plurality of analytes are simultaneously analyzed by collecting and recording images of the optical signals generated by all the microparticles in the planar array.
    Type: Grant
    Filed: April 4, 2014
    Date of Patent: March 1, 2016
    Assignee: Illumina, Inc.
    Inventors: John Bridgham, Kevin Corcoran, George Golda, Michael C. Pallas, Sydney Brenner
  • Publication number: 20150087530
    Abstract: Various embodiments of a low-volume sequencing system are provided herein. The system can include a low-volume flowcell having at least one reaction chamber of a defined volume (e.g., less than about 100 ?l). The system can also include an automated reagent delivery mechanism configured to reversibly couple with the inlet port corresponding to a target reaction chamber thereby placing allowing for reagent to be accurately moved from a storage container to the reaction chamber with minimal reagent waste. The flowcells can include a plurality of reaction chambers (e.g., 6) thereby allowing for parallel analysis of multiple samples. Various methods of analyzing a biomolecule are also provided herein.
    Type: Application
    Filed: September 26, 2014
    Publication date: March 26, 2015
    Inventors: Jon A. HOSHIZAKI, Joon Mo YANG, Maryam SHARIATI, David M. COX, Kirk M. HIRANO, John BRIDGHAM, George Stefan GOLDA, Sam Lee WOO
  • Publication number: 20150065356
    Abstract: According to various embodiments, a method is provided that comprises washing an array of DNA-coated beads on a substrate, with a wash solution to remove stacked beads from the substrate. The wash solution can include inert solid beads in a carrier. The DNA-coated beads can have an average diameter and the solid beads in the wash solution can have an average diameter that is at least twice the diameter of the DNA-coated beads. The washing can form dislodged DNA-coated beads and a monolayer of DNA-coated beads. In some embodiments, first beads for forming an array are contacted with a poly(ethylene glycol) (PEG) solution comprising a PEG having a molecular weight of about 350 Da or less. In some embodiments, slides for forming bead arrays are provided as are systems for imaging the same.
    Type: Application
    Filed: October 16, 2014
    Publication date: March 5, 2015
    Inventors: George A. FRY, Christina E. INMAN, John BRIDGHAM, Timothy HUNKAPILLER, Charles S. VANN
  • Patent number: 8889596
    Abstract: According to various embodiments, a method is provided that comprises washing an array of DNA-coated beads on a substrate, with a wash solution to remove stacked beads from the substrate. The wash solution can include inert solid beads in a carrier. The DNA-coated beads can have an average diameter and the solid beads in the wash solution can have an average diameter that is at least twice the diameter of the DNA-coated beads. The washing can form dislodged DNA-coated beads and a monolayer of DNA-coated beads. In some embodiments, first beads for forming an array are contacted with a poly(ethylene glycol) (PEG) solution comprising a PEG having a molecular weight of about 350 Da or less. In some embodiments, slides for forming bead arrays are provided as are systems for imaging the same.
    Type: Grant
    Filed: August 16, 2013
    Date of Patent: November 18, 2014
    Assignee: Life Technologies Corporation
    Inventors: George A. Fry, Christina E. Inman, John Bridgham, Timothy Hunkapiller, Charles S. Vann
  • Publication number: 20140213464
    Abstract: An apparatus and system are provided for simultaneously analyzing a plurality of analytes anchored to microparticles. Microparticles each having a uniform population of a single kind of analyte attached are disposed as a substantially immobilized planar array inside of a flow chamber where steps of an analytical process are carried out by delivering a sequence of processing reagents to the microparticles by a fluidic system under microprocessor control. In response to such process steps, an optical signal is generated at the surface of each microparticle which is characteristic of the interaction between the analyte carried by the microparticle and the delivered processing reagent. The plurality of analytes are simultaneously analyzed by collecting and recording images of the optical signals generated by all the microparticles in the planar array.
    Type: Application
    Filed: April 4, 2014
    Publication date: July 31, 2014
    Applicant: ILLUMINA, INC.
    Inventors: John Bridgham, Kevin Corcoran, George Golda, Michael C. Pallas, Sydney Brenner
  • Patent number: 8728729
    Abstract: An apparatus and system are provided for simultaneously analyzing a plurality of analytes anchored to microparticles. Microparticles each having a uniform population of a single kind of analyte attached are disposed as a substantially immobilized planar array inside of a flow chamber where steps of an analytical process are carried out by delivering a sequence of processing reagents to the microparticles by a fluidic system under microprocessor control. In response to such process steps, an optical signal is generated at the surface of each microparticle which is characteristic of the interaction between the analyte carried by the microparticle and the delivered processing reagent. The plurality of analytes are simultaneously analyzed by collecting and recording images of the optical signals generated by all the microparticles in the planar array.
    Type: Grant
    Filed: December 4, 2012
    Date of Patent: May 20, 2014
    Assignee: Illumina, Inc.
    Inventors: John Bridgham, Kevin Corcoran, George Golda, Michael C. Pallas, Sydney Brenner
  • Publication number: 20130331276
    Abstract: According to various embodiments, a method is provided that comprises washing an array of DNA-coated beads on a substrate, with a wash solution to remove stacked beads from the substrate. The wash solution can include inert solid beads in a carrier. The DNA-coated beads can have an average diameter and the solid beads in the wash solution can have an average diameter that is at least twice the diameter of the DNA-coated beads. The washing can form dislodged DNA-coated beads and a monolayer of DNA-coated beads. In some embodiments, first beads for forming an array are contacted with a poly(ethylene glycol) (PEG) solution comprising a PEG having a molecular weight of about 350 Da or less. In some embodiments, slides for forming bead arrays are provided as are systems for imaging the same.
    Type: Application
    Filed: August 16, 2013
    Publication date: December 12, 2013
    Applicant: LIFE TECHNOLOGIES CORPORATION
    Inventors: Mark F. OLDHAM, George A. FRY, Christina E. INMAN, John BRIDGHAM, Timothy HUNKAPILLER, Charles S. VANN
  • Patent number: 8536099
    Abstract: According to various embodiments, a method is provided that comprises washing an array of DNA-coated beads on a substrate, with a wash solution to remove stacked beads from the substrate. The wash solution can include inert solid beads in a carrier. The DNA-coated beads can have an average diameter and the solid beads in the wash solution can have an average diameter that is at least twice the diameter of the DNA-coated beads. The washing can form dislodged DNA-coated beads and a monolayer of DNA-coated beads. In some embodiments, first beads for forming an array are contacted with a poly(ethylene glycol) (PEG) solution comprising a PEG having a molecular weight of about 350 Da or less. In some embodiments, slides for forming bead arrays are provided as are systems for imaging the same.
    Type: Grant
    Filed: August 31, 2010
    Date of Patent: September 17, 2013
    Assignee: Life Technologies Corporation
    Inventors: Mark Oldham, George Fry, Christina Inman, John Bridgham, Timothy Hunkapillar, Charles Vann
  • Patent number: 8361713
    Abstract: An apparatus and system are provided for simultaneously analyzing a plurality of analytes anchored to microparticles. Microparticles each having a uniform population of a single kind of analyte attached are disposed as a substantially immobilized planar array inside of a flow chamber where steps of an analytical process are carried out by delivering a sequence of processing reagents to the microparticles by a fluidic system under microprocessor control. In response to such process steps, an optical signal is generated at the surface of each microparticle which is characteristic of the interaction between the analyte carried by the microparticle and the delivered processing reagent. The plurality of analytes are simultaneously analyzed by collecting and recording images of the optical signals generated by all the microparticles in the planar array.
    Type: Grant
    Filed: October 12, 2007
    Date of Patent: January 29, 2013
    Assignee: Illumina, Inc.
    Inventors: John Bridgham, Kevin Corcoran, George Golda, Michael C. Pallas, Sydney Brenner
  • Publication number: 20110136677
    Abstract: According to various embodiments, a method is provided that comprises washing an array of DNA-coated beads on a substrate, with a wash solution to remove stacked beads from the substrate. The wash solution can include inert solid beads in a carrier. The DNA-coated beads can have an average diameter and the solid beads in the wash solution can have an average diameter that is at least twice the diameter of the DNA-coated beads. The washing can form dislodged DNA-coated beads and a monolayer of DNA-coated beads. In some embodiments, first beads for forming an array are contacted with a poly(ethylene glycol) (PEG) solution comprising a PEG having a molecular weight of about 350 Da or less. In some embodiments, slides for forming bead arrays are provided as are systems for imaging the same.
    Type: Application
    Filed: August 31, 2010
    Publication date: June 9, 2011
    Applicant: LIFE TECHNOLOGIES CORPORATION
    Inventors: Mark OLDHAM, George Fry, Christina Inman, John Bridgham, Timothy Hunkapillar, Charles Vann
  • Publication number: 20110124111
    Abstract: Various embodiments of a low-volume sequencing system are provided herein. The system can include a low-volume flowcell having at least one reaction chamber of a defined volume (e.g., less than about 100 ?l). The system can also include an automated reagent delivery mechanism configured to reversibly couple with the inlet port corresponding to a target reaction chamber thereby placing allowing for reagent to be accurately moved from a storage container to the reaction chamber with minimal reagent waste. The flowcells can include a plurality of reaction chambers (e.g., 6) thereby allowing for parallel analysis of multiple samples. Various methods of analyzing a biomolecule are also provided herein.
    Type: Application
    Filed: August 31, 2010
    Publication date: May 26, 2011
    Applicant: LIFE TECHNOLOGIES CORPORATION
    Inventors: Jon HOSHIZAKI, Joon Mo YANG, Maryam SHARIATI, David COX, Kirk HIRANO, John BRIDGHAM, Sam WOO, George GOLDA
  • Publication number: 20110052446
    Abstract: Various flowcell configurations and systems are provided as are methods of making and using same. The flowcells, systems, and methods of use can be useful in carrying out sequencing reactions and next generation sequencing methods.
    Type: Application
    Filed: August 31, 2010
    Publication date: March 3, 2011
    Applicant: LIFE TECHNOLOGIES CORPORATION
    Inventors: Kirk HIRANO, Mark ANDERSEN, Jian GONG, Sam WOO, David COX, Joon Mo YANG, Min YUE, Maryam SHARIATI, John BRIDGHAM, David LIU
  • Publication number: 20100111768
    Abstract: The present invention comprises systems and devices for sequencing of nucleic acid, such as short DNA sequences from clonally amplified single-molecule arrays.
    Type: Application
    Filed: March 30, 2007
    Publication date: May 6, 2010
    Applicant: SOLEXA, INC.
    Inventors: Saibal Banerjee, Colin Barnes, Kevin Benson, John Bridgham, Jason Bryant, Dale Buermann, Sergey Etchin, Jonny Ho, Xavier Lee, Peter Lundberg, Klaus Maisinger, Bojan Obradovic, Mark Pratt, Isabelle Rasolonjatovo, Mark Reed, Chiara Rodighiero, Subra Sankar, Gary Schroth, Ning Sizto, Harold Swerdlow, Eric Vermaas
  • Publication number: 20090143244
    Abstract: An apparatus and system are provided for simultaneously analyzing a plurality of analytes anchored to microparticles. Microparticles each having a uniform population of a single kind of analyte attached are disposed as a substantially immobilized planar array inside of a flow chamber where steps of an analytical process are carried out by delivering a sequence of processing reagents to the microparticles by a fluidic system under microprocessor control. In response to such process steps, an optical signal is generated at the surface of each microparticle which is characteristic of the interaction between the analyte carried by the microparticle and the delivered processing reagent. The plurality of analytes are simultaneously analyzed by collecting and recording images of the optical signals generated by all the microparticles in the planar array.
    Type: Application
    Filed: October 12, 2007
    Publication date: June 4, 2009
    Inventors: John Bridgham, Kevin Corcoran, George Golda, Michael C. Pallas, Sydney Brenner
  • Publication number: 20090023189
    Abstract: Methods and systems for forming water-in-oil emulsions are described. For example, an apparatus is described which includes: a first compartment containing a plurality of particles dispersed in an aqueous phase; a second compartment containing an oil phase; a porous layer separating the first and second compartments; and a device for applying pressure to the first compartment. A method is described which includes: moving an oil phase relative to a surface of a porous layer while simultaneously forcing an aqueous composition comprising particles through the porous layer and into the flowing dispersion medium thereby forming droplets of the aqueous composition containing particles dispersed in the oil phase. The aqueous composition can include one or more nucleic acid templates and reagents for amplifying the nucleic acids such as PCR reagents.
    Type: Application
    Filed: May 12, 2008
    Publication date: January 22, 2009
    Applicant: APPLERA CORPORATION
    Inventors: Aldrich N.K. Lau, Jonathan M. Cassel, John A. Bridgham
  • Patent number: 7282370
    Abstract: An apparatus and system are provided for simultaneously analyzing a plurality of analytes anchored to microparticles. Microparticles each having a uniform population of a single kind of analyte attached are disposed as a substantially immobilized planar array inside of a flow chamber where steps of an analytical process are carried out by delivering a sequence of processing reagents to the microparticles by a fluidic system under microprocessor control. In response to such process steps, an optical signal is generated at the surface of each microparticle which is characteristic of the interaction between the analyte carried by the microparticle and the delivered processing reagent. The plurality of analytes are simultaneously analyzed by collecting and recording images of the optical signals generated by all the microparticles in the planar array.
    Type: Grant
    Filed: August 18, 2005
    Date of Patent: October 16, 2007
    Assignee: Solexa, Inc.
    Inventors: John Bridgham, Kevin Corcoran, George Golda, Michael C. Pallas, Sydney Brenner
  • Publication number: 20060051876
    Abstract: An apparatus and system are provided for simultaneously analyzing a plurality of analytes anchored to microparticles. Microparticles each having a uniform population of a single kind of analyte attached are disposed as a substantially immobilized planar array inside of a flow chamber where steps of an analytical process are carried out by delivering a sequence of processing reagents to the microparticles by a fluidic system under microprocessor control. In response to such process steps, an optical signal is generated at the surface of each microparticle which is characteristic of the interaction between the analyte carried by the microparticle and the delivered processing reagent. The plurality of analytes are simultaneously analyzed by collecting and recording images of the optical signals generated by all the microparticles in the planar array.
    Type: Application
    Filed: August 18, 2005
    Publication date: March 9, 2006
    Inventors: John Bridgham, Kevin Corcoran, George Golda, Michael Pallas, Sydney Brenner