Abstract: System, including methods, apparatus, and compositions, for performing amplification assays with an amplification reporter including a first oligomer and a second oligomer capable of base-pairing with one another below a melting temperature of the reporter. The reporter may have a detectable photoluminescence that is affected, such as reduced, by base-pairing of the first and second oligomers with one another. A target, such as a nucleic acid target sequence, may be amplified in at least one volume, such as a plurality of partitions, above the melting temperature, and photoluminescence of the reporter may be detected from the at least one volume below the melting temperature. A property of the target, such as a concentration of the target, may be determined based on the photoluminescence detected.

Abstract: System, including methods, apparatus, and compositions, for performing amplification assays with an amplification reporter including a first oligomer and a second oligomer capable of base-pairing with one another below a melting temperature of the reporter. The reporter may have a detectable photoluminescence that is affected, such as reduced, by base-pairing of the first and second oligomers with one another. A target, such as a nucleic acid target sequence, may be amplified in at least one volume, such as a plurality of partitions, above the melting temperature, and photoluminescence of the reporter may be detected from the at least one volume below the melting temperature. A property of the target, such as a concentration of the target, may be determined based on the photoluminescence detected.

Abstract: Method of haplotype analysis. In an exemplary method, an aqueous phase containing nucleic acid may be partitioned into a plurality of discrete volumes. At least one allele sequence may be amplified in the volumes from each of a first polymorphic locus and a second polymorphic locus that exhibit sequence variation in the nucleic acid. At least one measure of co-amplification of allele sequences from both loci in the same volumes may be determined. A haplotype of the first and second loci may be selected based on the at least one measure of co-amplification.

Abstract: Provided herein are improved methods, compositions, and kits for analysis of nucleic acids. The improved methods, compositions, and kits can enable copy number estimation of a nucleic acid in a sample. Also provided herein are methods, compositions, and kits for determining the linkage of two or more copies of a target nucleic acid in a sample (e.g. whether the two or more copies are on the same chromosome or different chromosomes) or for phasing alleles.

Abstract: Digital assay system, including methods, apparatus, and compositions, for performing target assays in partitions each containing a generic reporter and a specific reporter for target amplification.

Abstract: System, including methods, apparatus, and compositions, for performing amplification assays with an amplification reporter including a first oligomer and a second oligomer capable of base-pairing with one another below a melting temperature of the reporter. The reporter may have a detectable photoluminescence that is affected, such as reduced, by base-pairing of the first and second oligomers with one another. A target, such as a nucleic acid target sequence, may be amplified in at least one volume, such as a plurality of partitions, above the melting temperature, and photoluminescence of the reporter may be detected from the at least one volume below the melting temperature. A property of the target, such as a concentration of the target, may be determined based on the photoluminescence detected.

Abstract: System, including methods, apparatus, and compositions, for performing amplification assays with an amplification reporter including a first oligomer and a second oligomer capable of base-pairing with one another below a melting temperature of the reporter. The reporter may have a detectable photoluminescence that is affected, such as reduced, by base-pairing of the first and second oligomers with one another. A target, such as a nucleic acid target sequence, may be amplified in at least one volume, such as a plurality of partitions, above the melting temperature, and photoluminescence of the reporter may be detected from the at least one volume below the melting temperature. A property of the target, such as a concentration of the target, may be determined based on the photoluminescence detected.

Abstract: Digital assay system, including methods, apparatus, and compositions, for performing target assays in partitions each containing a generic reporter and a specific reporter for target amplification.

Abstract: Provided herein are improved methods, compositions, and kits for analysis of nucleic acids. The improved methods, compositions, and kits can enable copy number estimation of a nucleic acid in a sample. Also provided herein are methods, compositions, and kits for determining the linkage of two or more copies of a target nucleic acid in a sample (e.g. whether the two or more copies are on the same chromosome or different chromosomes) or for phasing alleles.

Abstract: Digital assay system, including methods, apparatus, and compositions, for performing target assays in partitions each containing a generic reporter and a specific reporter for target amplification.

Abstract: System, including methods, apparatus, and compositions, for performing amplification assays with an amplification reporter including a first oligomer and a second oligomer capable of base-pairing with one another below a melting temperature of the reporter. The reporter may have a detectable photoluminescence that is affected, such as reduced, by base-pairing of the first and second oligomers with one another. A target, such as a nucleic acid target sequence, may be amplified in at least one volume, such as a plurality of partitions, above the melting temperature, and photoluminescence of the reporter may be detected from the at least one volume below the melting temperature. A property of the target, such as a concentration of the target, may be determined based on the photoluminescence detected.

Abstract: Digital assay system, including methods, apparatus, and compositions, for performing target assays in partitions each containing a generic reporter and a specific reporter for target amplification.

Abstract: One-part, ambient moisture-curable polyurethane adhesives are produced by reacting with a polyol selected from the group consisting of polyether diols, triols and tetrols based on propylene glycol or ethylene-capped propylene glycol and mixtures thereof with an organic polyisocyanate for a sufficient time to form a reaction product including an isocyanate-terminated prepolymer and unreacted isocyanate, admixing with the reaction product talc or another additive containing free water which reacts with the unreacted isocyanate to extend the chain of the polyurethane and provide hard segments therein, allowing the water-isocyanate reaction to proceed until a predetermined amount of unreacted isocyanate remains and introducing the resulting adhesive into a substantially hermetically sealed container or package.

Abstract: A switching regulator with controlled simulated load has a control which connects and disconnects the simulated load to the output of the regulator. The control is responsive to an error signal of a first comparator. A second comparator receives the output of said first comparator and produces a control signal for the simulated load when the regulator output reaches a predetermined level.

Abstract: An improved electronic hybrid for coupling signals between two wire and four wire transmission circuits. The hybrid includes a pair of amplifiers associated with a pair of terminating impedances which are serially connected between the amplifier outputs and the two wire port. The amplifiers driving the two wire port through the terminating impedances are biased to couple a dc potential to the two wire line whereby the terminating impedances serve as battery feed resistors. Each amplifier-impedance arrangement includes a feedback circuit connected from the junction between the impedance and the two wire port and an input of the amplifier for providing positive feedback for the amplifier so that the output impedance of the hybrid is maintained at a desired level using battery feed resistors of smaller value than heretofore required. The result is the ability to drive longer loops through the smaller series impedances while at the same time exhibiting the desired terminating impedance.

Abstract: In a system of parallel switching regulators sharing a common load, each regulator generates controllable pulses from an unregulated d-c. voltage source and then smoothing those pulses to form a d-c. output that varies according to changes in the pulses. A comparator controls the pulses according to the differential between a reference signal and a feedback signal, and an output resistor is connected between the comparator and the load. A reference signal source generates a reference signal for the comparator in a first one of the regulators, and the load side of the output resistor in the first regulator is connected to the feedback signal input to the comparator in that regulator so that the load voltage is determined by the reference signal for the first regulator.

Abstract: A digital conferencing arrangement for use in a digital telecommunications switching system. Digital coded signals representative of sampled analog signals are compared and the results of the comparison noted to identify the present speaker in the conference. A comparison result indicating a proposed new speaker initiates a delay interval during which subsequent samples are compared to assure that the proposed new speaker indeed has the largest analog signal during the delay window. Upon verification of the proposed new speaker, the control of the conference circuit is transferred to him.

Abstract: An apparatus and method for testing for faults in an analog-to-digital (A/D) section and a digital-to-analog (D/A) section of a code converter in a digital time division, multiplex telecommunications system are disclosed. The A/D section is tested by connecting a digital test pattern via the switching network to the D/A section and monitoring for faults by comparing the resulting output to a reference value. The A/D section is tested by interconnecting the A/D section to be tested between a first previously tested D/A section and a second previously tested D/A section and then connecting the digital test pattern to the first D/A section while monitoring the output of the second D/A section for faults.

Abstract: A voice-frequency RC-active filter comprising a plurality of resistors and generalized impedance converter networks for synthesizing the frequency response of a preselected LC filter. Each generalized impedance converter network includes a pair of operational amplifiers and a plurality of impedances Z1, Z2, Z3, Z4 and Z5 interconnected with the amplifiers to provide the network with a driving point impedance ##EQU1## in which at least one of the impedances in both the numerator and the denominator of Zi includes a film-type resistor. At least one of the film-type resistors included in the impedances Z1, Z2, Z3, Z4 and Z5 is functionally trimmed to tune the filter. The capacitors in the filter are preferably discrete polycarbonate capacitors with a capacitance tolerance of .+-.5%, and all having the same value.

Abstract: A telephone system usable for example in hotels or the like and in which each of a plurality of telephone sets is equipped with a special status indicator or signal light as well as the usual bell, the system being characterized in that the indicator is automatically excited without ringing the bell by voltages applied from the ring generator whose output signal is connected across the tip and ring leads of a telephone line when the latter is placed in a special status (message waiting) by a status signal in the control unit. The ring generator is constructed to produce special status indicator excitation voltages during the "bell silent" intervals of its output signal, and the indicator is periodically actuated by connecting that output signal across the tip and ring leads of telephone sets, which are in the special status, during at least a portion of such intervals.