Abstract: A medical imaging system provides simultaneous rendering of visible light and fluorescent images. The system may employ dyes in a small-molecule form that remain in a subject's blood stream for several minutes, allowing real-time imaging of the subject's circulatory system superimposed upon a conventional, visible light image of the subject. The system may provide an excitation light source to excite the fluorescent substance and a visible light source for general illumination within the same optical guide used to capture images. The system may be configured for use in open surgical procedures by providing an operating area that is closed to ambient light. The systems described herein provide two or more diagnostic imaging channels for capture of multiple, concurrent diagnostic images and may be used where a visible light image may be usefully supplemented by two or more images that are independently marked for functional interest.

Abstract: Methods for analyzing biomedical data include: (a) obtaining macroscopic imaging data; (b) obtaining histopathological imaging data; (c) executing a parallel algorithm stored on a non-transient computer-readable medium to compute one or a plurality of network cycle features of a relative neighborhood graph derived from the histopathological imaging data; (d) registering the macroscopic imaging data and the histopathological imaging data; and (e) correlating the macroscopic imaging data and the network cycle features. Systems for analyzing biomedical data and computer readable storage media are described.

Abstract: An image mosaicking method includes performing pairwise registration of a plurality of tiles (101), determining absolute homographies for each of the plurality of tiles according to the pairwise registration (102B), and performing a blending of the plurality of tiles to obtain a stitched image according to the absolute homographies (103)

Abstract: A medical imaging system provides simultaneous rendering of visible light and diagnostic or functional images. The system may be portable, and may include adapters for connecting various light sources and cameras in open surgical environments or laparascopic or endoscopic environments. A user interface provides control over the functionality of the integrated imaging system. In one embodiment, the system provides a tool for surgical pathology.

Abstract: Macroscopic imaging data, such as from a CT, MR, PET, or SPECT scanner, is obtained. Microscopic imaging data of at least a portion of the same tissue is obtained. To align the microscopic imaging data with the macroscopic imaging data, intermediate data is also obtained. For example, photographic data is acquired at an intermediary stage of a process of preparing tissue for microscopic scan. The macroscopic and microscopic data are registered to the intermediary photographic data. Once registered to the intermediary data, the spatial relationship between the macroscopic and microscopic data is known and may be used for imaging or quantification.

Abstract: The present invention relates to methods for imaging annulus fibrosus tissue and a quantitative measurement of its local concentration in a sample using an agent comprising the compound of Formula I, a 13C or 2H enriched compound of Formula I, an 19F-labeled derivative of Formula I, a metal-DOTA (1,4,7,10-tetra-azacyclododecane-N,N?,N?,N??-tetraacetic acid) complex of Formula I, or a radioisotope derivative of Formula I.

Abstract: A medical imaging system provides simultaneous rendering of visible light and fluorescent images. The system may employ dyes in a small-molecule form that remains in a subject's blood stream for several minutes, allowing real-time imaging of the subject's circulatory system superimposed upon a conventional, visible light image of the subject. The system may also employ dyes or other fluorescent substances associated with antibodies, antibody fragments, or ligands that accumulate within a region of diagnostic significance. In one embodiment, the system provides an excitation light source to excite the fluorescent substance and a visible light source for general illumination within the same optical guide that is used to capture images. In another embodiment, the system is configured for use in open surgical procedures by providing an operating area that is closed to ambient light.

Abstract: A method for unsupervised classification of histological images of prostatic tissue includes providing histological image data obtained from a slide simultaneously co-stained with NIR fluorescent and Hematoxylin-and-Eosin (H&E) stains, segmenting prostate gland units in the image data, forming feature vectors by computing discriminating attributes of the segmented gland units, and using the feature vectors to train a multi-class classifier, where the classifier classifies prostatic tissue into benign, prostatic intraepithelial neoplasia (PIN), and Gleason scale adenocarcinoma grades 1 to 5 categories.

Abstract: The present invention relates to compositions for and methods of optically imaging tissues or cells using imaging agents having desirable in vivo properties that result in improved signal-to-background ratio.

Abstract: The present invention is directed to a non-isotopic methods for the in vitro and in vivo detection of hydroxyapatite-positive cells and structures.

Abstract: Described are methods of visualizing biological samples using histological staining and invisible light (e.g., infrared or near-infrared) fluorescence.

Abstract: Macroscopic imaging data, such as from a CT, MR, PET, or SPECT scanner, is obtained. Microscopic imaging data of at least a portion of the same tissue is obtained. To align the microscopic imaging data with the macroscopic imaging data, intermediate data is also obtained. For example, photographic data is acquired at an intermediary stage of a process of preparing tissue for microscopic scan. The macroscopic and microscopic data are registered to the intermediary photographic data. Once registered to the intermediary data, the spatial relationship between the macroscopic and microscopic data is known and may be used for imaging or quantification.

Abstract: Radio-labeled materials, e.g., compounds and compositions, methods of making the radio-labeled materials, and applications of the same are disclosed. For example, novel solid-state methods are disclosed that produce radio-labeled compositions that include a reactive, but stable radio-labeled compound in a polar anhydrous solvent. The radio-labeled compounds can be readily conjugated with a variety of ligands.

Abstract: An imaging device captures both a visible light image and a diagnostic image, the diagnostic image corresponding to emissions from an imaging medium within the object. The visible light image (which may be color or grayscale) and the diagnostic image may be superimposed to display regions of diagnostic significance within a visible light image. A number of imaging media may be used according to an intended application for the imaging device, and an imaging medium may have wavelengths above, below, or within the visible light spectrum. The devices described herein may be advantageously packaged within a single integrated device or other solid state device, and/or employed in an integrated, single-camera medical imaging system, as well as many non-medical imaging systems that would benefit from simultaneous capture of visible-light wavelength images along with images at other wavelengths.

Abstract: A medical imaging system provides simultaneous rendering of visible light and fluorescent images. The system may employ dyes in a small-molecule form that remain in a subject's blood stream for several minutes, allowing real-time imaging of the subject's circulatory system superimposed upon a conventional, visible light image of the subject. The system may provide an excitation light source to excite the fluorescent substance and a visible light source for general illumination within the same optical guide used to capture images. The system may be configured for use in open surgical procedures by providing an operating area that is closed to ambient light. The systems described herein provide two or more diagnostic imaging channels for capture of multiple, concurrent diagnostic images and may be used where a visible light image may be usefully supplemented by two or more images that are independently marked for functional interest.

Abstract: An imaging device captures both a visible light image and a diagnostic image, the diagnostic image corresponding to emissions from an imaging medium within the object. The visible light image (which may be color or grayscale) and the diagnostic image may be superimposed to display regions of diagnostic significance within a visible light image. A number of imaging media may be used according to an intended application for the imaging device, and an imaging medium may have wavelengths above, below, or within the visible light spectrum. The devices described herein may be advantageously packaged within a single integrated device or other solid state device, and/or employed in an integrated, single-camera medical imaging system, as well as many non-medical imaging systems that would benefit from simultaneous capture of visible-light wavelength images along with images at other wavelengths.

Abstract: Novel dyes, precursors to novel dyes, and conjugates of the novel dyes are disclosed, as well as methods of making and using the same.

Abstract: The present invention relates to methods for imaging annulus fibrosus tissue and a quantitative measurement of its local concentration in a sample using an agent comprising the compound of Formula I, a 13C or 2H enriched compound of Formula I, an 19F-labeled derivative of Formula I, a metal-DOTA (1,4,7,10-tetra-azacyclododecane-N,N?,N?,N??-tetraacetic acid) complex of Formula I, or a radioisotope derivative of Formula I.

Abstract: The invention relates to imaging systems that include a coded aperture detection system and an optical detection system. The coded aperture detection system is configured to detect radiation emitted by a radionuclide present within an object and to provide a first detector signal from the detected radiation. The optical detection system is configured to detect optical radiation from the object and to provide a second detector signal from the detected optical radiation. The system also includes a processor configured to prepare first image data from the first detector signal, second image data from the second detector signal, and registered data indicative of a spatial relationship in at least one dimension between the first and second image data. The invention also includes methods of using the new systems, e.g., for sentinel lymph node mapping and tissue resection.

Abstract: Novel dyes, precursors to novel dyes, and conjugates of the novel dyes are disclosed, as well as methods of making and using the same.