Patents by Inventor Jon A. Wolff

Jon A. Wolff has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Polyampholytes for delivering polyions to a cell
    Publication number: 20070071823
    Abstract: An polyampholyte is utilized in a condensed polynucleotide complex for purposes of nucleic acid delivery to a cell. The complex can be formed with an appropriate amount of positive and/or negative charge such that the resulting complex can be delivered to the extravascular space and may be further delivered to a cell.
    Type: Application
    Filed: March 11, 2002
    Publication date: March 29, 2007
    Inventors: Jon Wolff, James Hagstrom, Vladimir Budker, Vladimir Trubetskoy
  • Endosomolytic Polymers
    Publication number: 20070036865
    Abstract: We describe pH-sensitive endosomolytic polymers, delivery particles containing pH-sensitive endosomolytic polymers. The described particles are capable of delivering polynucleotides to cells from the peripheral circulation with subsequent release from endosomes. The endosomolytic polymers are inactive outside the cell but disrupt membranes upon exposure to an acidified endosomal compartment.
    Type: Application
    Filed: September 19, 2006
    Publication date: February 15, 2007
    Applicant: MIRUS BIO CORPORATION
    Inventors: David Rozema, Darren Wakefield, Jon Wolff, Vladimir Budker, Tatyana Budker, Sean Monahan, Vladimir Trubetskoy, James Hagstrom, Aaton Loomis, Paul Slattum
  • Methods for genetic immunization
    Publication number: 20070021364
    Abstract: The present invention relates to methods for delivering a genetic immunogen, comprising a nucleic acid capable of expressing an antigen, optionally complexed with a polymer. The nucleic acid is delivered to the host via hydrodynamic intravascular injection resulting in expression of an encoded antigen in extravascular cells and induction of an antigen-specific immune response.
    Type: Application
    Filed: May 19, 2006
    Publication date: January 25, 2007
    Inventors: Hans Herweijer, Jon Wolff, Mary Bates, Aaron Loomis, Vladimir Trubetskoy
  • Micellar systems
    Publication number: 20070010004
    Abstract: Methods are described for modifying nucleic acids to facilitate delivery of the nucleic acids to cells. Compounds which interact with of modify nucleic acids are interacted with the nucleic acids within reverse micelles.
    Type: Application
    Filed: June 30, 2006
    Publication date: January 11, 2007
    Inventors: Sean Monahan, Vladimir Budker, Tatyana Budker, Jon Wolff, Paul Slattum, James Hagstrom
  • Intravascular delivery of non-viral nucleic acid
    Patent number: 7148205
    Abstract: Disclosed is a process for transfecting genetic material into a mammalian cell to alter endogenous properties of the cell. The process comprises designing a polynucleotide for transfection. Then the polynucleotide is inserted into a mammalian vessel such as a tail vein or artery. Prior to insertion, subsequent to insertion, or concurrent with insertion the permeability of the vessel is increased thereby the genetic material is delivered to the parenchymal cell altering endogenous properties of the cell.
    Type: Grant
    Filed: June 20, 2003
    Date of Patent: December 12, 2006
    Assignee: Mirus Bio Corporation
    Inventors: Sean D. Monahan, Jon A. Wolff, Paul M. Slattum, James E. Hagstrom, Vladimir G. Budker, David B. Rozema
  • Nucleic acid injected into hapatic vein lumen and delivered to primate liver
    Patent number: 7144869
    Abstract: Processes are described for obtaining high levels of gene expression in primates after injection of nucleic acid to the liver via the lumen of the hepatic vein. The described process results in high level of gene expression with transient increases in liver enzymes.
    Type: Grant
    Filed: December 5, 2002
    Date of Patent: December 5, 2006
    Assignee: Mirus Bio Corporation
    Inventors: Jon A. Wolff, Julia Hegge, James E. Hagstrom, Vladimir G. Budker
  • Compositions and methods for drug delivery using pH sensitive molecules
    Patent number: 7138382
    Abstract: A system relating to the delivery of desired compounds (e.g., drugs and nucleic acids) into cells using pH-sensitive delivery systems. The system provides compositions and methods for the delivery and release of a compound to a cell.
    Type: Grant
    Filed: July 15, 2003
    Date of Patent: November 21, 2006
    Assignee: Mirus Bio Corporation
    Inventors: Jon A. Wolff, James E. Hagstrom, David B. Rozema, Sean D. Monahan, Vladimir G. Budker
  • Compositions and processes using siRNA, amphipathic compounds and polycations
    Patent number: 7101995
    Abstract: Described is a deliverable composition with low toxicity comprising an amphipathic compound, a polycation, and a siRNA. The composition may be used in the process of delivering a siRNA to an animal cell or more particularly, a mammal cell.
    Type: Grant
    Filed: May 28, 2002
    Date of Patent: September 5, 2006
    Assignee: Mirus Bio Corporation
    Inventors: David L. Lewis, James E. Hagstrom, Hans Herweijer, Aaron G. Loomis, Sean D. Monahan, Jon A. Wolff
  • Polyampholytes for delivering polyions to a cell
    Patent number: 7098030
    Abstract: An polyampholyte is utilized in a condensed polynucleotide complex for purposes of nucleic acid delivery to a cell. The complex can be formed with an appropriate amount of positive and/or negative charge such that the resulting complex can be delivered to the extravascular space and may be further delivered to a cell.
    Type: Grant
    Filed: July 26, 2004
    Date of Patent: August 29, 2006
    Assignee: Mirus Bio Corporation
    Inventors: David B. Rozema, Vladimir G. Budker, James E. Hagstrom, Vladimir Trubetskoy, Jon A. Wolff, Sean D. Monahan, Paul M. Slattum
  • Compositions and methods for drug delivery using pH sensitive molecules
    Patent number: 7098032
    Abstract: An polyampholyte is utilized in a condensed polynucleotide complex for purposes of nucleic acid delivery to a cell. The complex can be formed with an appropriate amount of positive and/or negative charge such that the resulting complex can be delivered to the extravascular space and may be further delivered to a cell.
    Type: Grant
    Filed: January 28, 2005
    Date of Patent: August 29, 2006
    Assignee: Mirus Bio Corporation
    Inventors: Vladimir S. Trubetskoy, James E. Hagstrom, Vladimir G. Budker, Jon A. Wolff, David B. Rozema, Sean D. Monahan
  • Nucleic acid injected into hapatic vein lumen and delivered to primate liver
    Publication number: 20060189553
    Abstract: Processes are described for obtaining high levels of gene expression in primates after injection of nucleic acid to the liver via the lumen of the hepatic vein. The described process results in high level of gene expression with transient increases in liver enzymes.
    Type: Application
    Filed: December 5, 2002
    Publication date: August 24, 2006
    Inventors: Jon Wolff, Julia Hegge, James Hagstrom, Vladimir Budker
  • Compounds and processes for single-pot attachment of a label to nucleic acid
    Publication number: 20060188927
    Abstract: Compounds and methods are provided for a single-pot covalent attachment of a label to nucleic acids comprising forming a covalently attachable labeling reagent for alkylating the molecule. Then, combining the covalently attachable labeling reagent with a mixture containing the molecule, under conditions wherein the labeling reagent has reactivity with the molecule thereby forming a covalent bond.
    Type: Application
    Filed: May 1, 2006
    Publication date: August 24, 2006
    Inventors: Paul Slattum, Jon Wolff, James Hagstrom, Vladimir Budker
  • Formation of polyampholytes in the presence of a polyion
    Patent number: 7094605
    Abstract: Polyampholyte are able to condense nucleic acid to form small complexes which can be utilized in the delivery of nucleic acid to mammalian cells. The polyampholytes can be formed prior to interaction with nucleic acid or they can be formed in the presence of nucleic acid. Stabilized polycation/nucleic acid complexes can be modified to reduce the positive charge of the polycation and add targeting ligands without destabilizing the complex. The resultant particles retain their small size and are more effective in delivery of nucleic acid to cells in vivo.
    Type: Grant
    Filed: January 28, 2005
    Date of Patent: August 22, 2006
    Assignee: Mirus Bio Corporation
    Inventors: Darren H. Wakefield, David B. Rozema, Jon A. Wolff, Vladimir Trubetskoy, James E. Hagstrom, Vladimir G. Budker, Jason Klein, So Wong
  • Micellar systems
    Patent number: 7091041
    Abstract: A complex is described that is deliverable to a cell comprising inserting a nucleic acid or other cargo into a reverse micelle. The reverse micelle has the property to compact the nucleic acid for easier delivery.
    Type: Grant
    Filed: July 25, 2003
    Date of Patent: August 15, 2006
    Assignee: Mirus Bio Corporation
    Inventors: Sean D. Monahan, Jon A. Wolff, Paul M. Slattum, James E. Hagstrom, Vladimir G. Budker
  • Compound containing a labile disulfide bond
    Patent number: 7087770
    Abstract: A labile disulfide-containing compound under physiological conditions containing a labile disulfide bond and a transduction signal.
    Type: Grant
    Filed: February 8, 2001
    Date of Patent: August 8, 2006
    Assignee: Mirus Bio Corporation
    Inventors: Jon A. Wolff, Sean D. Monahan, Vladimir G. Budker, Paul M. Slattum, David B. Rozema
  • Compounds and processes for single-pot attachment of a label to siRNA
    Publication number: 20060167239
    Abstract: Compounds and methods are provided for a single-pot covalent attachment of a label to an siRNA comprising forming a covalently attachable labeling reagent for alkylating the molecule. Then, combining the covalently attachable labeling reagent with a mixture containing the molecule, under conditions wherein the labeling reagent has reactivity with the molecule thereby forming a covalent bond.
    Type: Application
    Filed: January 20, 2006
    Publication date: July 27, 2006
    Inventors: Paul Slattum, Jon Wolff, Vladimir Budker, James Hagstorm
  • Process of making a compound by forming a polymer from a template drug
    Publication number: 20060159764
    Abstract: A method of forming polymers in the presence of nucleic acid using template polymerization. These methods can be used for the delivery of nucleic acids, for condensing the nucleic acid, for forming nucleic acid binding polymers, for forming supramolecular complexes containing nucleic acid and polymer, and for forming an interpolyelectrolyte complex.
    Type: Application
    Filed: December 30, 2005
    Publication date: July 20, 2006
    Inventors: Sean Monahan, David Rozema, Vladimir Trubetskoy, Paul Slattum, Jon Wolff, Vladimir Budker, James Hagstrom, Lisa Hanson
  • Compounds for targeting hepatocytes
    Publication number: 20060154867
    Abstract: We describe compounds that bind to and are internalized by hepatocytes. Association of these compounds to other molecules or complexes can be used to target the molecules or complexes to hepatocytes in vivo or in vitro.
    Type: Application
    Filed: January 20, 2006
    Publication date: July 13, 2006
    Inventors: Alexander Sokoloff, So Wong, Jon Wolff, Sean Monahan, James Ludtke, Lori Higgs, Darren Wakefield, Magdolna Sebestyen
  • Compounds for targeting hepatocytes in vivo
    Patent number: 7071163
    Abstract: We describe compounds that bind to and are internalized by hepatocytes. Association of these compounds to other molecules or complexes can be used to target the molecules or complexes to hepatocytes in vivo or in vitro.
    Type: Grant
    Filed: August 4, 2003
    Date of Patent: July 4, 2006
    Assignee: Mirus Bio Corporation
    Inventors: Alexander V. Sokoloff, So Wong, Jon A. Wolff, Sean D. Monahan, James Ludtke, Lori Higgs, Darren Wakefield, Magdolna G. Sebestyén
  • Reversible modification of amine-containing compounds
    Publication number: 20060122096
    Abstract: An process for the reversible modification of an amine-containing compound is described. Modification of the compound can be used to facilitate delivery of molecules to cells in vitro and in vivo or to alter interactions or activities the compounds. The described modifiers can also be utilized as cross-linkers.
    Type: Application
    Filed: December 20, 2005
    Publication date: June 8, 2006
    Inventors: David Rozema, Darren Wakefield, Jon Wolff, Kirk Ekena, James Hagstrom