Patents by Inventor Jon Wolff

Jon Wolff has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Endosomolytic Modified Poly(Alcohol) and Poly(Amine) Polymers
    Publication number: 20080281044
    Abstract: The present invention is directed to membrane active polymers useful for cellular delivery of compounds. Described are polyconjugates systems that incorporate targeting, anti-opsonization, anti-aggregation, and transfection activities into small biocompatible in vivo delivery conjugates. The use of reversible modification provides for physiologically responsive activity modulation.
    Type: Application
    Filed: June 17, 2008
    Publication date: November 13, 2008
    Inventors: Sean D. Monahan, Lori Higgs, Darren H. Wakefield, David B. Rozema, Jon A. Wolff, James E. Hagstrom
  • Endosomolytic Poly-Beta-Aminoester Polymers
    Publication number: 20080269450
    Abstract: The present invention is directed to membrane active polymers useful for cellular delivery of compounds. Described are polyconjugates systems that incorporate targeting, anti-opsonization, anti-aggregation, and transfection activities into small biocompatible in vivo delivery conjugates. The use of reversible modification provides for physiologically responsive activity modulation.
    Type: Application
    Filed: June 17, 2008
    Publication date: October 30, 2008
    Inventors: Darren H. Wakefield, David B. Rozema, Sean D. Monahan, Andrei Blokhin, Lori Higgs, Jon A. Wolff, James E. Hagstrom
  • Reversible modification of amine-containing compounds
    Patent number: 7442764
    Abstract: An process for the reversible modification of an amine-containing compound is described. Modification of the compound can be used to facilitate delivery of molecules to cells in vitro and in vivo or to alter interactions or activities the compounds. The described modifiers can also be utilized as cross-linkers.
    Type: Grant
    Filed: December 20, 2005
    Date of Patent: October 28, 2008
    Assignee: Mirns Bio Corporation
    Inventors: David B. Rozema, Darren Wakefield, Jon A. Wolff, Kirk Ekena, James E. Hagstrom
  • Process for utilizing epitopes recognized by natural antibodies
    Patent number: 7439051
    Abstract: The present invention relates to compositions and methods for the selection and use of surface exposed epitopes. The present invention includes in vivo and in vitro phage peptide diplay methods for the identification and selection of peptides and peptide associated factors with desired properties (e.g., targeting specificity, stability, etc.). The present invention further provides methods and compositions for the isolation and identification of peptide-specific antibodies. The present invention also includes methods and compositions employing nuclear localization signals for enhanced nuclear transport and expression of DNA.
    Type: Grant
    Filed: March 8, 2004
    Date of Patent: October 21, 2008
    Assignee: Mirus Bio Corporation
    Inventors: Alexander V. Sokoloff, Jon A. Wolff
  • Charge Reversal of Polyion Complexes
    Publication number: 20080234222
    Abstract: An ionic polymer is utilized in “recharging” (another layer having a different charge) a condensed polynucleotide complex for purposes of nucleic acid delivery to a cell. The resulting recharged complex can be formed with an appropriate amount of positive or negative charge such that the resulting complex has the desired net charge.
    Type: Application
    Filed: June 3, 2008
    Publication date: September 25, 2008
    Inventors: Jon A. Wolff, Vladimir S. Trubetskoy, Aaron G. Loomis, Paul M. Slattum, Sean D. Monahan, James E. Hagstrom, Vladimir G. Budker
  • Intravascular delivery of nucleic acid
    Patent number: 7396821
    Abstract: Disclosed is a process for transfecting genetic material into a mammalian cell to alter endogenous properties of the cell. The process comprises designing a polynucleotide for transfection. Then the polynucleotide is inserted into a mammalian blood vessel. Prior to insertion, subsequent to insertion, or concurrent with insertion the permability of the vessel is increased using an exterior cuff and immunosuppression is applied thereby the genetic material is delivered to the parenchymal cell.
    Type: Grant
    Filed: November 6, 2000
    Date of Patent: July 8, 2008
    Assignee: Miris Bio Corporation
    Inventors: Jon A. Wolff, Vladimir G. Budker
  • Charge reversal of polyion complexes
    Patent number: 7396919
    Abstract: An ionic polymer is utilized in “recharging” (another layer having a different charge) a condensed polynucleotide complex for purposes of nucleic acid delivery to a cell. The resulting recharged complex can be formed with an appropriate amount of positive or negative charge such that the resulting complex has the desired net charge.
    Type: Grant
    Filed: June 9, 1999
    Date of Patent: July 8, 2008
    Assignee: Mirus Bio Corporation
    Inventors: Jon A. Wolff, Vladimir S. Trubetskoy, Aaron G. Loomis, Paul M. Slattum, Sean D. Monahan, James E. Hagstrom, Vladimir G. Budker
  • Polyconjugates for In Vivo Delivery of Polynucleotides
    Publication number: 20080152661
    Abstract: The present invention is directed to compounds, compositions, and methods useful for delivering polynucleotides or other cell-impermeable molecules to mammalian cells. Described are polyconjugates systems that incorporate targeting, anti-opsonization, anti-aggregation, and transfection activities into small biocompatible in vivo delivery vehicles. The use of multiple reversible linkages connecting component parts provides for physiologically responsive activity modulation.
    Type: Application
    Filed: August 17, 2007
    Publication date: June 26, 2008
    Inventors: David B. Rozema, Darren H. Wakefield, Jason Klein, So Wong, Jon A. Wolff, David L. Lewis, James E. Hagstrom, Andrei Blokhin, Vladimir Trubetskoy, Hans Herweijer
  • Compounds and processes for single-pot attachment of a label to nucleic acid
    Patent number: 7326780
    Abstract: Compounds and methods are provided for a single-pot covalent attachment of a label to nucleic acids comprising forming a covalently attachable labeling reagent for alkylating the molecule. Then, combining the covalently attachable labeling reagent with a mixture containing the molecule, under conditions wherein the labeling reagent has reactivity with the molecule thereby forming a covalent bond.
    Type: Grant
    Filed: May 1, 2006
    Date of Patent: February 5, 2008
    Assignee: Mirus Bio Corporation
    Inventors: Paul M. Slattum, Jon A. Wolff, James E. Hagstrom, Vladimir G. Budker
  • DEVICES AND PROCESSES FOR DISTRIBUTION OF GENETIC MATERIAL TO MAMMALIAN LIMB
    Publication number: 20070244067
    Abstract: A process is described for the delivery of a therapeutic polynucleotide to limb muscle tissue suffering from or potentially suffering from Muscular Dystrophy. The polynucleotide is inserted into a mammalian limb vessel such as an artery. Delivery efficiency and distribution is enhanced by combining injection of a solution containing the polynucleotide with the use of an externally applied cuff.
    Type: Application
    Filed: March 30, 2007
    Publication date: October 18, 2007
    Inventors: Vladimir Budker, Jon Wolff
  • Lipid-Mediated Polynucleotide Administration to Deliver a Biologically Active Peptide and to Induce a Cellular Immune Response
    Publication number: 20070218077
    Abstract: A method for delivering a naked or isolated polynucleotide to the interior of a cell in a vertebrate, comprising the interstitial introduction of a naked polynucleotide into a tissue of the vertebrate where the polynucleotide is taken up by the cells of the tissue and exerts a therapeutic effect on the vertebrate. The method can be used to deliver a therapeutic polypeptide to the cells of the vertebrate, to provide an immune response upon in vivo translation of the polynucleotide, to deliver antisense polynucleotides, to deliver receptors to the cells of the vertebrate, or to provide transitory gene therapy.
    Type: Application
    Filed: May 16, 2007
    Publication date: September 20, 2007
    Applicants: VICAL INCORPORATED, Wisconsin Alumni Research Foundation
    Inventors: Phillip FELGNER, Jon Wolff, Gary Rhodes, Robert Malone, Dennis Carson
  • Chelating Compound for Drug Delivery
    Publication number: 20070219363
    Abstract: Chelator containing compounds are utilized in the delivery of molecules and polymers to animal cells. At least one chelator such as crown ether is attached to a polymer and then associated with another polymer. An ion is then added to the mixture thereby forming condensed polymer. In condensed form and in complex with the chelator, polymer can be delivered to a cell.
    Type: Application
    Filed: April 20, 2007
    Publication date: September 20, 2007
    Inventors: Vladimir Budker, Jon Wolff, Sean Monahan, Paul Slattum, James Hagstrom, Vladimir Trubetskoy, Tatyana Budker
  • Enhancing intermolecular integration of nucleic acids using integrator complexes
    Patent number: 7262056
    Abstract: We disclose compositions and processes for enhancing transposon mediated integration of a nucleic acid molecule into another target nucleic acid molecule. Integration by an integrator complex is enhanced by cationic reagents.
    Type: Grant
    Filed: November 8, 2002
    Date of Patent: August 28, 2007
    Assignee: Mirus Bio Corporation
    Inventors: Christine Wooddell, Hans Herweijer, Jon A. Wolff
  • Compound containing a labile disulfide bond
    Publication number: 20070191281
    Abstract: A labile disulfide-containing compound under physiological conditions containing a labile disulfide bond and a transduction signal.
    Type: Application
    Filed: October 12, 2005
    Publication date: August 16, 2007
    Inventors: Jon Wolff, Sean Monahan, Vladimir Budker, Paul Slattum, David Rozema
  • Lipid-mediated polynucleotide administration to deliver a biologically active peptide and to induce a cellular immune response
    Patent number: 7250404
    Abstract: A method for delivering an isolated polynucleotide to the interior of a cell in a vertebrate, comprising the interstitial introduction of an isolated polynucleotide into a tissue of the vertebrate where the polynucleotide is taken up by the cells of the tissue and exerts a therapeutic effect on the vertebrate. The method can be used to deliver a therapeutic polypeptide to the cells of the vertebrate, to provide an immune response upon in vivo translation of the polynucleotide, to deliver antisense polynucleotides, to deliver receptors to the cells of the vertebrate, or to provide transitory gene therapy.
    Type: Grant
    Filed: July 26, 2002
    Date of Patent: July 31, 2007
    Assignees: Vical Incorporated, Wisconsin Alumni Research Foundation
    Inventors: Philip L Felgner, Jon A Wolff, Gary H Rhodes, Robert Wallace Malone, Dennis A. Carson
  • Expression of zeta negative and zeta positive nucleic acids using a dystrophin gene
    Publication number: 20070173465
    Abstract: Disclosed is expression of zeta negative and zeta positive nucleic acids or nucleic acid complexes using a dystrophin gene in a process for providing nucleic acid expression in a striated (skeletal or cardiac) muscle cell for the purpose of providing a change to the endogenous properties of the cell for cells affected by muscular dystrophy.
    Type: Application
    Filed: January 10, 2003
    Publication date: July 26, 2007
    Inventors: Sean Monahan, Jon Wolff, Paul Slattum, James Hagstrom, Vladimir Budker, David Rozema
  • Inhibition of gene expression by delivery of polynucleotides to animal cells in vivo
    Publication number: 20070128169
    Abstract: Described is a process for intravascular delivery of a polynucleotide to an extravascular cell of a mammal to inhibit gene expression. A polynucleotide containing sequence that is similar to a sequence in the gene to be expressed is made and inserted into a vessel in the mammal. The polynucleotide is delivered to a cell wherein expression of the gene is inhibited.
    Type: Application
    Filed: January 4, 2007
    Publication date: June 7, 2007
    Inventors: David Lewis, Jon Wolff, Vladimir Budker, Hans Herweijer, James Hagstrom
  • Endosomolytic Polymers
    Publication number: 20070105804
    Abstract: Processes are described for obtaining high levels of gene expression in primates after injection of nucleic acid to the liver via the lumen of the hepatic vein. The described process results in high level of gene expression with transient increases in liver enzymes.
    Type: Application
    Filed: October 10, 2006
    Publication date: May 10, 2007
    Applicant: MIRUS BIO CORPORATION
    Inventors: Jon Wolff, Julia Hegge, James Hagstrom, Vladimir Budker
  • Devices and processes for distribution of genetic material to mammalian limb
    Patent number: 7214369
    Abstract: A process is described for the delivery of a therapeutic polynucleotide to limb muscle tissue suffering from or potentially suffering from ischemia. The polynucleotide is inserted into a mammalian limb vessel such as an artery. Delivery efficiency and distribution is enhanced by combining injection of a solution containing the polynucleotide with the use of an externally applied cuff.
    Type: Grant
    Filed: May 5, 2003
    Date of Patent: May 8, 2007
    Assignee: Mirus Bio Corporation
    Inventors: Jon A. Wolff, Vladimir G. Budker, James E. Hagstrom, Julia Hegge, Vladimir Subbotin
  • Compositions and methods for drug delivery using pH sensitive molecules
    Patent number: 7208314
    Abstract: A system relating to the delivery of desired compounds (e.g., drugs and nucleic acids) into cells using pH-sensitive delivery systems. The system provides compositions and methods for the delivery and release of a compound to a cell.
    Type: Grant
    Filed: February 26, 2002
    Date of Patent: April 24, 2007
    Assignee: Mirus Bio Corporation
    Inventors: Sean D. Monahan, Jon A. Wolff, James E. Hagstrom, Vladimir G. Budker, David B. Rozema