Abstract: Process for the production of human TAF in vitro comprising growing the human colon adenocarcinoma cell line HT-29 in agitated, liquid suspension of nutrient culture medium at about 35.degree.-38.degree. C. for a sufficient time to elaborate TAF and recovering the resulting TAF from the cells or cell product.

Abstract: Process for the production of human TAF in vitro comprising growing the human osteosarcoma cell line MG-63 in agitated, liquid suspension of nutrient culture medium at about 35.degree.-38.degree. C. for a sufficient time to elaborate TAF and recovering the resulting TAF from the cells or cell product.

Abstract: Process for the production of human TAF in vitro comprising growing the human Burkitt lymphoma cell line Raji in agitated, liquid suspension of nutrient culture medium at about 35.degree.-38.degree. C. for a sufficient time to elaborate TAF and recovering the resulting TAF from the cells or cell product.

Abstract: Process for the production of human TAF in vitro comprising growing the human medulloblastoma cell line TE-671 in agitated, liquid suspension of nutrient culture medium at about 35.degree.-38.degree. C. for a sufficient time to elaborate TAF and recovering the resulting TAF from the cells or cell product.

Abstract: Process for the production of human TAF in vitro comprising growing the human rhabdomyosarcoma cell line RD in agitated, liquid suspension of nutrient culture medium at about 35.degree.-38.degree. C. for a sufficient time to elaborate TAF and recovering the resulting TAF from the cells or cell product.

Abstract: Process for the production of human TAF in vitro comprising growing the human melanoma cell line A-375 in agitated, liquid suspension of nutrient culture medium at about 35.degree.-38.degree. C. for a sufficient time to elaborate TAF and recovering the resulting TAF from the cells or cell product.

Abstract: Process for the production of human TAF in vitro comprising growing the human liver adenocardinoma cell line SK-HEP-1 in agitated, liquid suspension of nutrient culture medium at about 35.degree.-38.degree. C. for a sufficient time to elaborate TAF and recovering the resulting TAF from the cells or cell product.

Abstract: A cell culture reactor is provided for the growth of cells in vitro which employs elongate, selectively permeable hollow fibers arranged in a shallow layer configuration as a matrix for cell attachment on the outer surface of the fibers, and aeration of the cells by passage through the interior of said fibers and permeation of the membrane cell. The flow path of the culture media is directed by distributor plate means substantially uniformly through the fiber layer and substantially transverse to the plane of the elongate axes of the fibers.

Abstract: The yield of urokinase produced by cell culture of kidney cells is improved by incorporating in the cell culture medium an elevated level of phenylalanine.

Abstract: A cell culture sampling device is provided which comprises a double septum holder having an axial bore therethrough, upper and lower septa retaining members, said upper retaining member having lip means to retain immobilized an external septum and said lower retaining member having shoulder means to retain immobilized an internal septum, said lip and shoulder means being spaced apart to thereby define a fixed chamber between said immobilized septa.

Abstract: A cell culture system and apparatus is provided for the suspension culturing of mammalian cells in which fresh media can be added and spent medium filtered and withdrawn on a continuous or semi-continuous basis without cell disruption. The apparatus comprises a hollow flask assembly having a hollow shaft means journaled for rotation in a stationary sleeve sub-assembly and a filter unit suspended downwardly therefrom, said filter unit being in fluid communication with the top opening of the flask through the interior of said hollow shaft means and adapted for coaxial rotation with said shaft means.

Abstract: A cell culture system and apparatus is provided for the suspension culturing of mammalian cells in which fresh media can be added and spent medium filtered and withdrawn on a continuous or semi-continuous basis without any substantial cell disruption and clogging of the filter with cell debris. The apparatus comprises a hollow flask assembly having a stationary hollow shaft means and filter unit suspended downwardly from the top thereof, and a rotatable agitator means concentrically disposed about said filter unit, said filter unit being in fluid communication with the top opening of the flask through the interior of said hollow shaft means.

Abstract: A cell culture system and apparatus is provided for the suspension culturing of mammalian cells in which fresh media can be added and spent medium filtered and withdrawn on a continuous or semi-continuous basis without cell disruption. The apparatus comprises a hollow flask assembly having a hollow shaft means journaled for rotation in a stationary sleeve sub-assembly and a filter unit suspended downwardly therefrom, said filter unit being in fluid communication with the top opening of the flask through the interior of said hollow shaft means and adapted for coaxial rotation with said shaft means.

Abstract: A cell culture reactor system is provided for the growth of cells in vitro which employs elongate hollow or solid fibers arranged in a shallow layer configuration as a matrix for cell attachment in which the flow path of the culture media is directed substantially uniformly through the fiber layer and substantially transverse to the plane of the elongate axes of the fibers.

Abstract: Densely packed cells growing rapidly in substrate-attached culture are gently released from the substrate by enzymatic treatment to form aggregates ranging from 20 .mu. to 500 .mu. in diameter. These aggregates are then transferred to fresh medium in agitated liquid suspension. The cells thereby continue to grow in aggregates whereby a normally anchorage-dependent estabished mammalian cell line is rapidly adapted to suspension culture.