Patents by Inventor Joseph G. Brewen

Joseph G. Brewen has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Displacement polynucleotide assay employing polyether and diagnostic kit
    Patent number: 4766064
    Abstract: A diagnostic reagent is disclosed containing a complex of a probe polynucleotide (P) bound via purine/pyrimidine hydrogen bonding to a labeled polynucleotide (L). The probe (P) contains a target binding region (TBR) capable of binding to a target nucleotide sequence (G) of a biological sample. A method is disclosed in which contact with a sample containing the target nucleotide sequence (G) causes binding, initially between G and a single-stranded portion (IBR) of the target binding region (TBR). Thereafter the labeled polynucleotide (L) is displaced from the complex by branch migration of (G) into the (P)/(L) binding region. A volume excluding polymeric agent such as poly(ethylene oxide) (PEO or PEG) or other polyethers enhances the rate of appearance of displaced labeled polynucleotide. Determination of displaced labeled polynucleotide (L) gives a value which is a function of the presence and concentration of target nucleotide sequence (G) in the sample.
    Type: Grant
    Filed: December 20, 1984
    Date of Patent: August 23, 1988
    Assignees: Allied Corporation, Genetics Institute, Inc.
    Inventors: Jon I. Williams, Marian S. Ellwood, Mary Collins, Edward F. Fritsch, Joseph G. Brewen, Steven E. Diamond
  • Displacement polynucleotide assay method and polynucleotide complex reagent therefor
    Patent number: 4766062
    Abstract: A diagnostic reagent is disclosed containing a complex of a probe polynucleotide (P) bound via purine/pyrimidine hydrogen bonding to a labeled polynucleotide (L). The probe (P) contains a target binding region (TBR) capable of binding to a target nucleotide sequence (G) of a biological sample. A method is disclosed in which contact with a sample containing the target nucleotide sequence (G) causes binding, initially between G and a single-stranded portion (IBR) of the target binding region (TBR). Thereafter the labeled polynucleotide (L) is displaced from the complex by branch migration of (G) into the (P)/(L) binding region. Determination of displaced labeled polynucleotide (L) gives a value which is a function of the presence and concentration of target nucleotide sequence (G) in the sample.
    Type: Grant
    Filed: May 7, 1984
    Date of Patent: August 23, 1988
    Assignees: Allied Corporation, Genetics Institute, Inc.
    Inventors: Steven E. Diamond, Joseph G. Brewen, Jon I. Williams, Marian S. Ellwood, Mary Collins, Edward F. Fritsch