Patents by Inventor Joseph M. Jacobson

Joseph M. Jacobson has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Patent number: 10377080
    Abstract: A 3D object according to the invention comprises substrate layers infiltrated by a hardened material. The 3D object is fabricated by a method comprising the following steps: Position powder on all or part of a substrate layer. Repeat this step for the remaining substrate layers. Stack the substrate layers. Transform the powder into a substance that flows and subsequently hardens into the hardened material. The hardened material solidifies in a spatial pattern that infiltrates positive regions in the substrate layers and does not infiltrate negative regions in the substrate layers. In a preferred embodiment, the substrate is carbon fiber and excess substrate is removed by abrasion.
    Type: Grant
    Filed: November 3, 2017
    Date of Patent: August 13, 2019
    Assignee: Impossible Objects, Inc.
    Inventors: Robert Swartz, Buckley Crist, Eugene Gore, Joseph M. Jacobson
  • Patent number: 10377106
    Abstract: A 3D object according to the invention involves substrate layers infiltrated by a hardened material. The 3D object may be fabricated by a method comprising the following steps: Flatten a substrate layer. Position powder on all or part of a substrate layer. Repeat this step for the remaining substrate layers. Stack the substrate layers. Transform the powder into a substance that flows and subsequently hardens into the hardened material. The hardened material solidifies in a spatial pattern that infiltrates positive regions in the substrate layers and does not infiltrate negative regions in the substrate layers. In a preferred embodiment, the substrate is carbon fiber and excess substrate is removed by abrasion. Flattening a substrate layer involves reducing planar inconsistencies or imperfections, and comprises applying heat to each substrate layer, cooling the substrate layers, and optionally applying tension and/or pressure to the heated and cooled substrate layers.
    Type: Grant
    Filed: October 2, 2017
    Date of Patent: August 13, 2019
    Assignee: Impossible Objects, Inc.
    Inventors: Robert Swartz, Buckley Crist, Eugene Gore, Joseph M. Jacobson
  • Publication number: 20190218532
    Abstract: A Streptococcus canis Cas9 (ScCas9) ortholog and its engineered variants, possessing novel PAM specificity, is an addition to the family of CRISPR-Cas9 systems. ScCas9 endonuclease is used in complex with guide RNA, consisting of identical non-target-specific sequence to that of the guide RNA SpCas9, for specific recognition and activity on a DNA target immediately upstream of either an “NNGT” or “NNNGT” PAM sequence. A novel DNA-interacting loop domain within ScCas9, and other Cas9 orthologs, such as those from Streptococcus gordonii and Streptococcus angionosis facilitates a divergent PAM sequence from the “NGG” PAM of SpCas9.
    Type: Application
    Filed: September 19, 2018
    Publication date: July 18, 2019
    Applicant: Massachusetts Institute of Technology
    Inventors: Joseph M. Jacobson, Noah Michael Jakimo, Pranam Chatterjee
  • Publication number: 20180298391
    Abstract: A self-reconfiguring genome uses a cassette having operons or DNA sequences that code for guide RNA, reverse transcriptase, donor RNA, and a CRISPR cleavage enzyme. A self-reconfiguring genome may be based on lambda recombineering of in situ generated oligonucleotides. A method for programmable self-modification of a cellular genome includes transcribing guide RNA from a self-reconfiguring cassette, associating the transcribed guideRNA with the CRISPR enzyme, intercalcating a region of complimentary sequence within an integration site of the genome, cutting upstream of a PAM site within the integration site; transcribing the donorRNA, translating donorRNA to double-stranded DNA, and recombining the double-stranded DNA via homologous recombination at the cut site of the integration site.
    Type: Application
    Filed: February 26, 2018
    Publication date: October 18, 2018
    Applicant: Massachusetts Institute of Technology
    Inventors: Noah Jakimo, Peter A. Carr, Joseph M. Jacobson
  • Publication number: 20180282722
    Abstract: A chimeric DNA:RNA guide for very high accuracy Cas9 genome editing employs nucleotide-type substitutions in nucleic acid-guided endonucleases for enhanced specificity. The CRISPR-Cas9 gene editing system is manipulated to generate chimeric DNA:RNA guide strands to minimize the off-target cleavage events of the S. pyogenes Cas9 endonuclease. A DNA:RNA chimeric guide strand is sufficient to guide Cas9 to a specified target sequence for indel formation and minimize off-target cleavage events due to the specificity conferred by DNA-DNA interactions. Use of chimeric mismatch-evading lowered-thermostability guides (“melt-guides”) demonstrate that nucleotide-type substitutions in the spacer can reduce cleavage of sequences mismatched by as few as a single base pair. The chimeric mismatch-evading lowered-thermostability guides replace most gRNA spacer positions with DNA bases to suppress mismatched targets under Cas9's catalytic threshold.
    Type: Application
    Filed: November 21, 2017
    Publication date: October 4, 2018
    Applicant: Massachusetts Institute of Technology
    Inventors: Noah Jakimo, Pranam Chatterjee, Joseph M. Jacobson
  • Patent number: 10030253
    Abstract: A method for synthesizing long DNA constructs from oligonucleotide precursors directly within a microfluidic device uses several oligonucleotides at once. A precursor mix containing at least two oligonucleotide precursors with at least partial base complementarity is introduced into an input of a microfluidic chip and at least one cycle of at least one gene synthesis protocol is applied to fabricate a DNA construct containing the sequence of at least two oligonucleotide precursors. A method for the synthesis of a modified DNA construct includes electroporating at least one oligonucleotide encoding for at least one point mutation and having homology with at least one DNA region of a target cell into the target cell and incorporating the oligonucleotide into the target cell DNA through the action of recombination protein beta or a recombination protein beta functional homolog.
    Type: Grant
    Filed: October 30, 2014
    Date of Patent: July 24, 2018
    Assignee: Massachusetts Institute of Technology
    Inventors: David Kong, Peter A. Carr, Joseph M. Jacobson
  • Publication number: 20180141305
    Abstract: A 3D object according to the invention comprises substrate layers infiltrated by a hardened material. The 3D object is fabricated by a method comprising the following steps: Position powder on all or part of a substrate layer. Repeat this step for the remaining substrate layers. Stack the substrate layers. Transform the powder into a substance that flows and subsequently hardens into the hardened material. The hardened material solidifies in a spatial pattern that infiltrates positive regions in the substrate layers and does not infiltrate negative regions in the substrate layers. In a preferred embodiment, the substrate is carbon fiber and excess substrate is removed by abrasion.
    Type: Application
    Filed: August 25, 2015
    Publication date: May 24, 2018
    Applicant: IMPOSSIBLE OBJECTS LLC
    Inventors: Robert Swartz, Buckley Crist, Eugene Gore, Joseph M. Jacobson
  • Publication number: 20180126666
    Abstract: A 3D object according to the invention comprises substrate layers infiltrated by a hardened material. The 3D object is fabricated by a method comprising the following steps: Position powder on all or part of a substrate layer. Repeat this step for the remaining substrate layers. Stack the substrate layers. Transform the powder into a substance that flows and subsequently hardens into the hardened material. The hardened material solidifies in a spatial pattern that infiltrates positive regions in the substrate layers and does not infiltrate negative regions in the substrate layers. In a preferred embodiment, the substrate is carbon fiber and excess substrate is removed by abrasion.
    Type: Application
    Filed: August 25, 2015
    Publication date: May 10, 2018
    Applicant: IMPOSSIBLE OBJECTS LLC
    Inventors: Robert Swartz, Buckley Crist, Eugene Gore, Joseph M. Jacobson
  • Publication number: 20180050490
    Abstract: A 3D object according to the invention comprises substrate layers infiltrated by a hardened material. The 3D object is fabricated by a method comprising the following steps: Position powder on all or part of a substrate layer. Repeat this step for the remaining substrate layers. Stack the substrate layers. Transform the powder into a substance that flows and subsequently hardens into the hardened material. The hardened material solidifies in a spatial pattern that infiltrates positive regions in the substrate layers and does not infiltrate negative regions in the substrate layers. In a preferred embodiment, the substrate is carbon fiber and excess substrate is removed by abrasion.
    Type: Application
    Filed: November 3, 2017
    Publication date: February 22, 2018
    Inventors: Robert Swartz, Buckley Crist, Eugene Gore, Joseph M. Jacobson
  • Publication number: 20180022065
    Abstract: A 3D object according to the invention involves substrate layers infiltrated by a hardened material. The 3D object may be fabricated by a method comprising the following steps: Flatten a substrate layer. Position powder on all or part of a substrate layer. Repeat this step for the remaining substrate layers. Stack the substrate layers. Transform the powder into a substance that flows and subsequently hardens into the hardened material. The hardened material solidifies in a spatial pattern that infiltrates positive regions in the substrate layers and does not infiltrate negative regions in the substrate layers. In a preferred embodiment, the substrate is carbon fiber and excess substrate is removed by abrasion. Flattening a substrate layer involves reducing planar inconsistencies or imperfections, and comprises applying heat to each substrate layer, cooling the substrate layers, and optionally applying tension and/or pressure to the heated and cooled substrate layers.
    Type: Application
    Filed: October 2, 2017
    Publication date: January 25, 2018
    Inventors: Robert Swartz, Buckley Crist, Eugene Gore, Joseph M. Jacobson
  • Patent number: 9833949
    Abstract: A 3D object according to the invention comprises substrate layers infiltrated by a hardened material. The 3D object is fabricated by a method comprising the following steps: Position powder on all or part of a substrate layer. Repeat this step for the remaining substrate layers. Stack the substrate layers. Transform the powder into a substance that flows and subsequently hardens into the hardened material. The hardened material solidifies in a spatial pattern that infiltrates positive regions in the substrate layers and does not infiltrate negative regions in the substrate layers. In a preferred embodiment, the substrate is carbon fiber and excess substrate is removed by abrasion.
    Type: Grant
    Filed: August 25, 2015
    Date of Patent: December 5, 2017
    Assignee: Impossible Objects, Inc.
    Inventors: Robert Swartz, Buckley Crist, Eugene Gore, Joseph M. Jacobson
  • Publication number: 20170334133
    Abstract: A 3D object according to the invention comprises substrate layers infiltrated by a hardened material. The 3D object is fabricated by a method comprising the following steps: Position powder on all or part of a substrate layer. Repeat this step for the remaining substrate layers. Stack the substrate layers. Transform the powder into a substance that flows and subsequently hardens into the hardened material. The hardened material solidifies in a spatial pattern that infiltrates positive regions in the substrate layers and does not infiltrate negative regions in the substrate layers. In a preferred embodiment, the substrate is carbon fiber and excess substrate is removed by abrasion.
    Type: Application
    Filed: August 25, 2015
    Publication date: November 23, 2017
    Inventors: Robert Swartz, Buckley Crist, Eugene Gore, Joseph M. Jacobson
  • Patent number: 9776376
    Abstract: A 3D object according to the invention involves substrate layers infiltrated by a hardened material. The 3D object may be fabricated by a method comprising the following steps: Flatten a substrate layer. Position powder on all or part of a substrate layer. Repeat this step for the remaining substrate layers. Stack the substrate layers. Transform the powder into a substance that flows and subsequently hardens into the hardened material. The hardened material solidifies in a spatial pattern that infiltrates positive regions in the substrate layers and does not infiltrate negative regions in the substrate layers. In a preferred embodiment, the substrate is carbon fiber and excess substrate is removed by abrasion.
    Type: Grant
    Filed: May 4, 2015
    Date of Patent: October 3, 2017
    Assignee: Impossible Objects, LLC
    Inventors: Robert Swartz, Buckley Crist, Eugene Gore, Joseph M. Jacobson
  • Patent number: 9729182
    Abstract: A platform for context-aware experimentation includes a housing for one or more sensors for obtaining data pertaining to an on-going experiment, a communications subsystem for transmitting data obtained by the sensors, and a microcontroller for receiving data from the sensors, providing it to the communications subsystem, and possibly controlling the sensors. The housing may be a tube, which may be configured to hold a sample and may have a cap, or a waterproof package, which may have an opening to admit at least part of a sample. The platform may include a power source. The platform may include a computer processor, located outside the housing, for analyzing the data obtained by the sensors, determining the experimental context in which the sensors are operating and/or which experimental step in a protocol is being performed, and/or reminding users of required parameters for the steps in the protocol.
    Type: Grant
    Filed: April 28, 2015
    Date of Patent: August 8, 2017
    Assignee: Massachusetts Institute of Technology
    Inventors: Charles Victor Fracchia, Joseph M. Jacobson
  • Publication number: 20170190802
    Abstract: Fabrication and arrangement of nanoparticles into one-dimensional linear chains is achieved by successive chemical reactions, each reaction adding one or more nanoparticles by building onto exposed, unprotected linker functionalities. Optionally, protecting groups may be used to control and organize growth. Nanoparticle spheres are functionalized in a controlled manner in order to enable covalent linkages. Functionalization of nanoparticles is accomplished by either ligand exchange or chemical modification of the terminal functional groups of the capping ligand. Nanoparticle chains are obtained by a variety of connectivity modes such as direct coupling, use of linker molecules, and use of linear polymeric templates. In particular, a versatile building block system is obtained through controlled monofunctionalization of nanoparticles.
    Type: Application
    Filed: January 19, 2017
    Publication date: July 6, 2017
    Applicant: Massachusetts Institute of Technology
    Inventors: Joseph M. Jacobson, David W. Mosley, Kie-Moon Sung
  • Publication number: 20170168370
    Abstract: A dielectrophoretic display has a substrate having walls defining a cavity, the cavity having a viewing surface and a side wall inclined to the viewing surface. A fluid is contained within the cavity; and a plurality of particles are present in the fluid. There is applied to the substrate an electric field effective to cause dielectrophoretic movement of the particles so that the particles occupy only a minor proportion of the viewing surface.
    Type: Application
    Filed: February 27, 2017
    Publication date: June 15, 2017
    Inventors: Karl R. Amundson, Alexandros Cosmos Arango, Joseph M. Jacobson, Thomas H. Whitesides, Michael D. McCreary, Richard J. Paolini, JR.
  • Patent number: 9664978
    Abstract: A dielectrophoretic display comprises a substrate having walls defining at least one cavity, the cavity having a viewing surface and a side wall inclined to the viewing surface; a suspending fluid contained within the cavity; a plurality of at least one type of particle suspended within the suspending fluid; and means for applying to the fluid an electric field effective to cause dielectrophoretic movement of the particles to the side wall of the cavity.
    Type: Grant
    Filed: March 4, 2015
    Date of Patent: May 30, 2017
    Assignee: E Ink Corporation
    Inventors: Alexi C. Arango, Joseph M. Jacobson, Karl Raymond Amundson, Dirk Hertel
  • Publication number: 20170137858
    Abstract: In a method for generating an elongated nucleic acid molecule, a nucleic acid addition of a first nucleic acid molecule attached to a first 3? or 5? protecting group to a nucleic acid immobilized on a surface produces an intermediate-length immobilized nucleic acid. The first protecting group is dissociated from the first nucleic acid molecule. A second nucleic acid molecule that is attached to a second associated a 3? or 5? associated protecting group is added to the intermediate-length nucleic acid. The second associated protecting group is dissociated from the second nucleic acid molecule. A sequentially-extended elongated immobilized nucleic acid molecule having a desired sequence and length is produced by sequentially extending the intermediate-length immobilized nucleic acid by adding additional nucleic acid molecules with associated protecting groups to the intermediate-length nucleic acid and dissociating the associated protecting group after each addition.
    Type: Application
    Filed: November 22, 2016
    Publication date: May 18, 2017
    Applicant: Massachusetts Institute of Technology
    Inventors: Peter A. Carr, Brian Y. Chow, Joseph M. Jacobson, David W. Mosley, Christopher Emig
  • Patent number: 9499848
    Abstract: In a method for generating a long nucleic acid molecule, nucleic acids immobilized on a surface and having overlapping complementary sequences is released into solution. The overlapping complementary sequences are hybridized to form hybridized nucleic acids, followed by extension or ligation of the hybridized nucleic acids to synthesize the long nucleic acid molecule. The nucleic acids may comprise first and second series of nucleic acids having redundant overlapping sequences, wherein nucleic acids from the first and second series are complementary to each other. The complementary nucleic acids are hybridized to form the hybridized nucleic acids. The generated long nucleic acid molecule may have a predetermined sequence element, and it may be introduced into a system wherein the predetermined sequence element is required for replication, such that replication of the synthesized long nucleic acid molecule is indicative of the presence of the predetermined sequence element in the long nucleic acid molecule.
    Type: Grant
    Filed: August 13, 2013
    Date of Patent: November 22, 2016
    Assignee: Massachusetts Institute of Technology
    Inventors: Peter A. Carr, Brian Y. Chow, Joseph M. Jacobson, David W. Mosley, Christopher Emig
  • Publication number: 20160244784
    Abstract: Population-Hastened Assembly Genetic Engineering is a method for continuous genome recoding using a mixed population of cells. Nucleic acid donors are distributed amongst a population of cells that continuously transfer nucleic acids to achieve asynchronous recoding of genetic information within a subpopulation of the cells. Recombination is achieved with biochemical systems compatible with virtually any organism. An engineered directed endonuclease comprises a nucleic acid recognition domain, a nucleic acid endonuclease domain, and a linker fusing or causing interaction between the nucleic acid recognition domain and the nucleic acid endonuclease domain. The method includes causing at least one engineered directed endonuclease to create a nick in a nucleic acid strand, the nick being offset from the recognition sequence of the nucleic acid recognition domain; causing homologous recombination of the strand with a donor nucleotide to create a modified genome; and replicating the modified genome.
    Type: Application
    Filed: February 16, 2016
    Publication date: August 25, 2016
    Applicant: Massachusetts Institute of Technology
    Inventors: Joseph M. Jacobson, Noah Jakimo, Lisa Nip