Abstract: The present invention relates to an integrated biomaterial for bone tissue regeneration and a method of preparing the same, and more particularly to an integrated biomaterial for bone tissue regeneration, which includes a lower structure consisting of an extracellular matrix protein and a bone mineral and an upper layer consisting of an extracellular matrix protein. In the integrated biomaterial for bone tissue regeneration according to the present invention, the lower structure consisting of an extracellular matrix protein and a bone mineral component realizes a natural bone tissue environment, and thus facilitates the regeneration of new bone, and particularly, the upper layer consisting of an extracellular matrix protein is placed thereon at an appropriate ratio, and thus not only prevents the infiltration of epithelial tissue or connective tissue but also maximizes bone tissue regeneration capability.