Patents by Inventor Jufang Shi

Jufang Shi has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Publication number: 20100055680
    Abstract: Methods of amplifying genomic DNA are provided which include contacting template genomic DNA, a plurality of random primers, and a DNA polymerase, wherein the ratio of template genomic DNA to random primers is in the range of about 1:10-1:35,000,000 (w/w), inclusive, to produce a reaction mixture; and incubating the reaction mixture under isothermal conditions suitable for DNA synthesis, thereby producing amplified genomic DNA characterized by less than 10 percent gene copy number error. The template genomic DNA used in described methods can be in denatured condition and/or in non-denatured condition to achieve production of amplified genomic DNA characterized by less than 10 percent gene copy number error. In a particular option, the reaction mixture includes detectably labeled nucleotides and detectably labeled amplified genomic DNA characterized by less than 10 percent gene copy number error is produced.
    Type: Application
    Filed: August 28, 2008
    Publication date: March 4, 2010
    Applicant: PerkinElmer LAS, Inc.
    Inventor: Shirley Jufang Shi
  • Patent number: 6387626
    Abstract: The invention relates to a simple, cost effective method for immobilizing synthetic nucleic acid molecules onto a solid support The invention further concerns the use of such immobilized molecules in nucleic acid hybridization assays, sequencing by hybridization assays, and genetic analyses and combinatorial analyses involving nucleic acids or proteins for screening applications.
    Type: Grant
    Filed: August 14, 2000
    Date of Patent: May 14, 2002
    Assignee: Orchid BioSciences, Inc.
    Inventors: Jufang Shi, Michael T. Boyce-Jacino
  • Patent number: 6287821
    Abstract: The invention concerns a novel class of 3′-modified, pro-fluorescent nucleotides. The invention also pertains to methods for using such nucleotides.
    Type: Grant
    Filed: June 11, 1998
    Date of Patent: September 11, 2001
    Assignee: Orchid BioSciences, Inc.
    Inventors: Jufang Shi, Michael T. Boyce-Jacino, Phillip Goelet
  • Publication number: 20010018183
    Abstract: The invention comprises a multi-color, comparative hybridization assay method using an array of nucleic acid target elements attached to a solid support for the simultaneous detection of both gene expression and chromosomal abnormalities in a tissue sample. The method of the invention employs a comparative hybridization of a tissue mRNA or cDNA sample labeled in a first fluorescent color, a tissue chromosomal DNA sample labeled in a second fluorescent color, and at least one reference nucleic acid labeled in a third fluorescent color, to the array. The fluorescent color presence and intensity at each of at least two target elements are detected and the fluorescent ratios (i) of the first and third colors and (ii) the second and third colors determined. Gene expression and chromosomal abnormalities are thus simultaneously detected.
    Type: Application
    Filed: February 28, 2001
    Publication date: August 30, 2001
    Inventors: Yijia Bao, Diping Che, Wan-Liang Li, Uwe Richard Muller, Steven A. Seelig, Jufang Shi
  • Patent number: 6251601
    Abstract: The invention comprises a multi-color, comparative hybridization assay method using an array of nucleic acid target elements attached to a solid support for the simultaneous detection of both gene expression and chromosomal abnormalities in a tissue sample. The method of the invention employs a comparative hybridization of a tissue mRNA or cDNA sample labeled in a first fluorescent color, a tissue chromosomal DNA sample labeled in a second fluorescent color, and at least one reference nucleic acid labeled in a third fluorescent color, to the array. The fluorescent color presence and intensity at each of at least two target elements are detected and the fluorescent ratios (i) of the first and third colors and (ii) the second and third colors determined. Gene expression and chromosomal abnormalities are thus simultaneously detected.
    Type: Grant
    Filed: February 2, 1999
    Date of Patent: June 26, 2001
    Assignee: Vysis, Inc.
    Inventors: Yijia Bao, Diping Che, Wan-Liang Li, Uwe Richard Müller, Steven A. Seelig, Jufang Shi
  • Patent number: 6136962
    Abstract: This invention relates to a composition having a nucleic acid molecule covalently coupled to a solid support by means of a covalent linkage. The nucleic acid molecule is covalently coupled to the solid support by a silane. The composition can be used in nucleic acid hybridization assays and sequencing by hybridization assays.
    Type: Grant
    Filed: June 23, 1998
    Date of Patent: October 24, 2000
    Assignee: Orchid BioSciences, Inc.
    Inventors: Jufang Shi, Michael T. Boyce-Jacino
  • Patent number: 5919626
    Abstract: The invention relates to a simple, cost effective method for immobilizing synthetic nucleic acid molecules onto a solid support. The invention further concerns the use of such immobilized molecules in nucleic acid hybridization assays, sequencing by hybridization assays, and genetic analyses and combinatorial analyses involving nucleic acids or proteins for screening applications.
    Type: Grant
    Filed: June 6, 1997
    Date of Patent: July 6, 1999
    Assignee: Orchid Bio Computer, Inc.
    Inventors: Jufang Shi, Michael T. Boyce-Jacino