Patents by Inventor Junichi Mineno
Junichi Mineno has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Patent number: 8841126Abstract: Disclosed is a method for introducing a gene into a target cell using a retrovirus vector, which is simple and highly efficient. Specifically disclosed is a method for introducing a gene into a target cell using a retrovirus vector, which comprises the steps of (a) placing a liquor containing a retrovirus vector having a foreign gene carried thereon into a culture vessel on which a retrovirus-binding substance has been immobilized, and incubating the liquor at a temperature lower than 25° C. for 4 hours or longer, thereby producing a culture vessel having the retrovirus vector bound thereto, and (b) adding a target cell to the culture vessel that has been produced in step (a) and incubating the culture vessel. The gene introduction method is simple and highly efficient, and is useful particularly in the fields of medicine, cell technology, gene technology, embryologic technology and the like.Type: GrantFiled: June 29, 2011Date of Patent: September 23, 2014Assignee: Takara Bio Inc.Inventors: Katsuyuki Dodo, Naoki Saito, Hideto Chono, Junichi Mineno
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Publication number: 20140242701Abstract: Provided are a chimeric antigen receptor comprising an extracellular domain capable of binding to an antigen, a transmembrane domain and at least one intracellular domain, the chimeric antigen receptor being characterized in that an intracellular domain of a glucocorticoid-induced tumor necrosis factor receptor (GITR) is contained as the intracellular domain; a nucleic acid encoding the chimeric antigen receptor; a cell expressing the chimeric antigen receptor; and a method for producing the cell.Type: ApplicationFiled: October 5, 2012Publication date: August 28, 2014Applicant: TAKARA BIO INC.Inventors: Hiroshi Shiku, Yuki Orito, Junichi Mineno, Sachiko Okamoto, Yasunori Amaishi
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Patent number: 8815597Abstract: The present invention provides a baglike container for centrifugation that is mounted in a centrifuge to thereby allow centrifugation of a dispersion liquid accommodated therein. The baglike container for centrifugation is less likely to tear or break as a result of centrifugation by disposing a container wall surface of the baglike container so as to apply centrifugal force perpendicular to the container wall surface.Type: GrantFiled: August 23, 2007Date of Patent: August 26, 2014Assignee: Takara Bio Inc.Inventors: Hideto Chono, Junichi Mineno, Kazutoh Takesako, Takao Yoshida, Takashi Morimura, Kenji Sakai, Shin-ichi Yamada, Noritsugu Yabe, Yuko Taguchi
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Publication number: 20140170709Abstract: The present invention provides a retroviral vector containing a transcription unit comprising a transcription regulatory sequence and a gene encoding a polypeptide having single-stranded RNA-specific endoribonuclease activity which is placed so that its expression can be controlled by the regulatory sequence, wherein the unit is placed so that the direction of the transcription of mRNA from the unit is opposite to the direction of transcription of the RNA genome of the retroviral vector. By using the vector constructed as described above, viral supernatant showing high gene transfer efficiency can be prepared. The retroviral vector of the present invention is useful for the treatment and/or prevention of cancers and virus infections.Type: ApplicationFiled: January 31, 2014Publication date: June 19, 2014Applicant: TAKARA BIO INC.Inventors: Hideto CHONO, Kazuya MATSUMOTO, Hajime MATSUMURA, Junichi MINENO, Ikunoshin KATO
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Publication number: 20140120124Abstract: Disclosed are: a cell capable of expressing an exogenous GITRL or an exogenous GITRL derivative; a method for producing the cell; a therapeutic or prophylactic agent comprising the cell as an active ingredient; use of the cell in the manufacture of a therapeutic or prophylactic agent; a method comprising a step of administering the cell to a subject; a viral vector carrying a gene encoding a GITRL or a GITRL derivative; a therapeutic or prophylactic agent comprising the viral vector as an active ingredient; use of the viral vector in the manufacture of a therapeutic or prophylactic agent; and a method comprising a step of administering the viral vector to a subject.Type: ApplicationFiled: October 15, 2013Publication date: May 1, 2014Applicants: TAKARA BIO INC., MIE UNIVERSITYInventors: Hiroshi SHIKU, Hiroaki IKEDA, Jun MITSUI, Yuki TAKENAKA, Junichi MINENO, Ikunoshin KATO
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Patent number: 8586023Abstract: Disclosed are: a cell capable of expressing an exogenous GITRL or an exogenous GITRL derivative; a method for producing the cell; a therapeutic or prophylactic agent comprising the cell as an active ingredient; use of the cell in the manufacture of a therapeutic or prophylactic agent; a method comprising a step of administering the cell to a subject; a viral vector carrying a gene encoding a GITRL or a GITRL derivative; a therapeutic or prophylactic agent comprising the viral vector as an active ingredient; use of the viral vector in the manufacture of a therapeutic or prophylactic agent; and a method comprising a step of administering the viral vector to a subject.Type: GrantFiled: September 11, 2009Date of Patent: November 19, 2013Assignees: Mie University, Takara Bio Inc.Inventors: Hiroshi Shiku, Hiroaki Ikeda, Jun Mitsui, Yuki Takenaka, Junichi Mineno, Ikunoshin Kato
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Publication number: 20130183719Abstract: The present invention provides a method for producing a virus vector, which comprises a step wherein cells that are capable of producing a virus vector are cultured in a culture medium that contains, as active components, a retinoic acid and a histone deacetylase inhibiting substance; and a culture medium for the production of a virus vector, which is characterized by containing, as active components, a retinoic acid and a histone deacetylase inhibiting substance.Type: ApplicationFiled: October 4, 2011Publication date: July 18, 2013Inventors: Kazuhisa Shinmura, Yoshinori Katayama, Kensuke Sakai, Toshihiro Shodai, Hirofumi Yoshioka, Junichi Mineno
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Publication number: 20130142766Abstract: The present invention addresses the problem of providing cell populations having a high proportion of CD4-positive naive T cells and/or CD4-positive central memory T cells, and a production method thereof. The present invention provides a production method for CD4-positive T cell populations which is characterized by using anti-CD3 antibodies, fibronectin fragments, and Interleukin-4. The method is characterized not only by the attainment of a cell group with a high proportion of CD4-positive naive T cells and/or CD4-positive central memory T cells, but also by a high bulk yield.Type: ApplicationFiled: August 9, 2011Publication date: June 6, 2013Inventors: Katsuyuki Dodo, Hideto Chono, Junichi Mineno
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Publication number: 20130109096Abstract: Disclosed is a method for introducing a gene into a target cell using a retrovirus vector, which is simple and highly efficient. Specifically disclosed is a method for introducing a gene into a target cell using a retrovirus vector, which comprises the steps of (a) placing a liquor containing a retrovirus vector having a foreign gene carried thereon into a culture vessel on which a retrovirus-binding substance has been immobilized, and incubating the liquor at a temperature lower than 25° C. for 4 hours or longer, thereby producing a culture vessel having the retrovirus vector bound thereto, and (b) adding a target cell to the culture vessel that has been produced in step (a) and incubating the culture vessel. The gene introduction method is simple and highly efficient, and is useful particularly in the fields of medicine, cell technology, gene technology, embryologic technology and the like.Type: ApplicationFiled: June 29, 2011Publication date: May 2, 2013Inventors: Katsuyuki Dodo, Naoki Saito, Hideto Chono, Junichi Mineno
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Publication number: 20120142109Abstract: Disclosed is a method for producing a cell population containing memory-like T cells, said method being characterized by including a step for in vitro culturing of a cell population containing T cells or T cell precursor cells using a retinoic acid and a CD3 ligand. Further disclosed is a method for producing a cell population wherein the proportion of memory-like T cells is increased and wherein a desired gene is introduced with a high efficiency and is highly expressed.Type: ApplicationFiled: August 24, 2010Publication date: June 7, 2012Inventors: Yoshinori Katayama, Kensuke Sakai, Hirofumi Yoshioka, Hikaru Takakura, Hideto Chono, Naoki Saito, Junichi Mineno
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Publication number: 20120009162Abstract: Disclosed are: polypeptides for TCR ?-chain and ?-chain which are restricted to HLA-A*0201 for Aur-A and are derived from a CTL; and nucleic acids encoding the polypeptides. The nucleic acids can impart a cytotoxic activity against a cell capable of presenting an HLA-A*0201 molecule and an Aur-A207-215 peptide thereon to a T cell, and are therefore useful for the treatment of cancer in which Aur-A is expressed.Type: ApplicationFiled: April 2, 2010Publication date: January 12, 2012Inventors: Masaki Yasukawa, Hiroshi Fujiwara, Toshiki Ochi, Junichi Mineno, Ikunoshin Kato
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Publication number: 20110318839Abstract: Disclosed is a method for enhancing the function of a T cell, which is characterized by inhibiting the expression of programmed death-1 ligand 1 (PD-L1) and/or programmed death-1 ligand 2 (PD-L2) in the T cell. Also disclosed is a function-enhanced T cell which is produced by the function enhancement method. Further disclosed is a therapeutic agent comprising the function-enhanced T cell. The T cell can enhance an immune response to cancer, and is useful in an immunotherapy effective for cancer and the treatment or prevention of infectious diseases and autoimmune diseases.Type: ApplicationFiled: March 5, 2010Publication date: December 29, 2011Inventors: Hiroshi Shiku, Hiroaki Ikeda, Koichi Iwamura, Junichi Mineno, Ikunoshin Kato
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Publication number: 20110250230Abstract: Disclosed are: a cell capable of expressing an exogenous GITRL or an exogenous GITRL derivative; a method for producing the cell; a therapeutic or prophylactic agent comprising the cell as an active ingredient; use of the cell in the manufacture of a therapeutic or prophylactic agent; a method comprising a step of administering the cell to a subject; a viral vector carrying a gene encoding a GITRL or a GITRL derivative; a therapeutic or prophylactic agent comprising the viral vector as an active ingredient; use of the viral vector in the manufacture of a therapeutic or prophylactic agent; and a method comprising a step of administering the viral vector to a subject.Type: ApplicationFiled: September 11, 2009Publication date: October 13, 2011Inventors: Hiroshi Shiku, Hiroaki Ikeda, Jun Mitsui, Yuki Takenaka, Junichi Mineno, Ikunoshin Kato
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Publication number: 20110027242Abstract: Disclosed is a method for producing a cell population into which a desired gene is introduced, which is characterized by comprising the following steps (1) and (2): (1) culturing a cell population containing a T-cell and/or a progenitor cell of a T-cell in a vessel containing fibronectin or a fragment thereof and a CD3 ligand; and (2) adding a vector carrying the desired gene to the vessel of the step (1). By carrying out both the steps (1) and (2) in the same vessel, the gene transfer can be achieved with high efficiency.Type: ApplicationFiled: March 27, 2009Publication date: February 3, 2011Applicant: TAKARA BIO INC.Inventors: Ikuei Nukaya, Yumi Goto, Yasuhiro Tosaka, Junichi Mineno, Ikunoshin Kato
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Publication number: 20100273213Abstract: Disclosed is a cell which can express a non-natural oligomeric protein, which has, introduced therein, a gene encoding an exogenous polypeptide corresponding to at least one endogenous polypeptide constituting a natural oligomeric protein, and in which the expression of the endogenous polypeptide is inhibited.Type: ApplicationFiled: June 6, 2008Publication date: October 28, 2010Inventors: Junichi Mineno, Sachiko Okamoto, Risa Sumioka, Masanari Kitagawa, Hiroshi Shiku, Ikunoshin Kato
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Publication number: 20100137415Abstract: The present invention provides a retroviral vector containing a transcription unit comprising a transcription regulatory sequence and a gene encoding a polypeptide having single-stranded RNA-specific endoribonuclease activity which is placed so that its expression can be controlled by the regulatory sequence, wherein the unit is placed so that the direction of the transcription of mRNA from the unit is opposite to the direction of transcription of the RNA genome of the retroviral vector. By using the vector constructed as described above, viral supernatant showing high gene transfer efficiency can be prepared. The retroviral vector of the present invention is useful for the treatment and/or prevention of cancers and virus infections.Type: ApplicationFiled: April 16, 2008Publication date: June 3, 2010Applicant: TAKARA BIO INC.Inventors: Hideto Chono, Kazuya Matsumoto, Hajima Matsumura, Junichi Mineno, Ikunoshin Kato
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Publication number: 20100136680Abstract: Baglike container (1) that is fitted in a centrifuge to thereby allow centrifugation of any dispersion liquid placed therein. The baglike container for centrifugation is one comprising a main frame constituted of first container wall (3) with recess portion (6) formed so as to allow fitting in a centrifuge and with flange portion (7) provided on an open face border of the recess portion (6) and constituted of flexible second container wall (4) having its peripheral portion sealed mutually with the flange portion (7) of the first container wall (3) so as to define storing space (6?) for dispersion liquid in cooperation with the recess portion (6) and further comprising port (5) fitted to the main frame in a fashion allowing communication with the storing space (6?), wherein the recess portion (6) defining the storing space (6?) can be set in the centrifuge so that centrifugal force perpendicular to the bottom plane of the recess portion (6) applies.Type: ApplicationFiled: August 23, 2006Publication date: June 3, 2010Inventors: Hideto Chono, Junichi Mineno, Kazutoh Takesako, Takao Yoshida, Takashi Morimura, Kenji Sakai, Shin-ichi Yamada, Noritsugu Yabe, Yuko Taguchi
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Publication number: 20100113566Abstract: A nucleic acid comprising a transcription regulation sequence whose transcription is induced by a trans-acting factor of a human immunodeficiency virus and a gene encoding a polypeptide having an endoribonuclease activity specific to single-stranded RNA, wherein the gene is located in such a position that the expression of the gene can be regulated by the transcription regulation sequence; a method for production of a cell showing an inhibited replication of a human immunodeficiency virus therein, the method comprising the step of introducing the nucleic acid into a cell; and a method for treatment or prevention of a human immunodeficiency virus infection.Type: ApplicationFiled: August 10, 2006Publication date: May 6, 2010Inventors: Hideto Chono, Kazuya Matsumoto, Junichi Mineno, Ikunoshin Kato
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Publication number: 20090156420Abstract: To provide a method for selecting a marker gene useful for cancer classification; a method for classifying cancer using the gene; a method for detecting cancer; a kit usable for the classification method or detection method; and a DNA array carrying the gene. According to the present invention, there can be obtained a gene, wherein expression of the above gene is altered independently from genes each of which expression is altered specifically during cell proliferation and expression level of the above gene is specifically altered depending on every type of cancer samples to be tested, whereby the classification or detection of cancer can be carried out conveniently and quickly without giving surgical treatment. Therefore, the present invention is useful for the diagnosis, the treatment, and the like of cancer.Type: ApplicationFiled: August 25, 2008Publication date: June 18, 2009Inventors: Yasuhiro Tsubosa, Kazuhiko Aoyagi, Hiroki Sasaki, Masaaki Terada, Junichi Mineno, Kiyozo Asada, Ikunoshin Kato
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Patent number: 7485448Abstract: A method for obtaining a virus vector free of a serum, and a serum-free medium for cultivation of a virus producer cell which can be used for the method are provided. By cultivating a virus producer cell using a serum-free medium containing serum albumin, it is possible to cultivate the cell in a state equivalent to that in a serum-containing medium to produce a virus vector at a sufficient titer. The virus vector prepared from the virus producer cell cultivated in the medium exhibits gene transfer efficiency comparable to that of a conventional vector. The medium is useful for gene therapy and studies thereof.Type: GrantFiled: September 13, 2006Date of Patent: February 3, 2009Assignee: Takara Bio Inc.Inventors: Hirofumi Yoshioka, Yasushi Katayama, Daisuke Tomura, Masao Funakoshi, Kazuya Matsumoto, Junichi Mineno, Kazutoh Takesako, Ikunoshin Kato