Patents by Inventor Junzo Hasegawa
Junzo Hasegawa has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Patent number: 7329518Abstract: The present invention relates to a method of producing an optically active pyridineethanol derivative. More particularly, it relates to a method of producing an optically active polycyclic pyridineethanol derivative by causing an enzyme or enzyme source to act on polycyclic acetylpyridine derivatives. The present invention also relates to a novel enzyme which can be used in the production method mentioned above, a DNA coding for said enzyme, a recombinant vector having said DNA, and a transformant having said recombinant vector. The invention further relates to a method of producing an optically active polycyclic pyridineethanol derivative by causing the above novel enzyme or the above transformant to act on optically inactive polycyclic pyridineethanol derivatives.Type: GrantFiled: September 25, 2003Date of Patent: February 12, 2008Assignee: Kaneka CorporationInventors: Shigeru Kawano, Miho Horikawa, Yoshihiko Yasohara, Junzo Hasegawa
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Patent number: 7169592Abstract: To provide a method for preparing optically active compounds, which mainly contain (R)-amino compounds, by microbial enzymes efficiently and inexpensively; a polypeptide having stereoselective transaminase activity which can be suitably used for the above preparation method; and a DNA encoding the polypeptide. A method for preparing an optically active amino compound, characterized in stereoselectively transaminating by acting a transaminase on an amino group acceptor, a ketone compound in the presence of an amino group donor, a primary amine; a DNA comprising a nucleotide sequence encoding a polypeptide having stereoselective transaminase activity; and a polypeptide having stereoselective transaminase activity obtainable from a culture of a microorganism belonging to the genus Arthrobacter.Type: GrantFiled: April 2, 2002Date of Patent: January 30, 2007Assignee: Kaneka CorporationInventors: Yukio Yamada, Akira Iwasaki, Noriyuki Kizaki, Keiji Matsumoto, Yasuhiro Ikenaka, Masahiro Ogura, Junzo Hasegawa
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Patent number: 7033808Abstract: The present invention provides a novel polypeptide producing (S)-N-benzyl-3-pyrrolidinol, a DNA coding for it and a method of using them. A polypeptide having the following physicochemical properties (1) to (5): (1) Action: It asymmetrically reduces N-benzyl-3-pyrrolidinone to produce (S)-N-benzyl-3-pyrrolidinol with NADPH as a coenzyme; (2) Optimum action pH: 4.5 to 5.5; (3) Optimum action temperature: 40° C. to 45° C.; (4) Molecular weight: About 29,000 as determined by gel filtration analysis, about 35,000 as determined by SDS-polyacrylamide gel electrophoresis analysis; (5) Inhibitor: It is inhibited by the divalent copper ion.Type: GrantFiled: August 1, 2001Date of Patent: April 25, 2006Assignee: Kaneka CorporationInventors: Noriyuki Kizaki, Yoshihiko Yasohara, Junzo Hasegawa
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Patent number: 6762037Abstract: The present invention has for its object to isolate a gene coding for the enzyme synthesizing the coenzyme Q10 side chain from a fungal strain of the genus Saitoella and exploit it to advantage for the efficient microbial production of coenzyme Q10. The present invention provides; a DNA having the nucleotide sequence shown under SEQ ID NO:1 a DNA having a nucleotide sequence derived from the nucleotide sequence of SEQ ID NO:1 by the deletion, addition, insertion and/or substitution of one or a plurality of nucleotides and coding for a protein having decaprenyl diphosphate synthase activity a DNA which hybridizes with the DNA having the nucleotide sequence of SEQ ID NO:1 under a stringent condition and codes for a protein having decaprenyl diphosphate synthase activity.Type: GrantFiled: July 23, 2001Date of Patent: July 13, 2004Assignee: Kaneka CorporationInventors: Hideyuki Matsuda, Makoto Kawamukai, Kazuyoshi Yajima, Yasuhiro Ikenaka, Junzo Hasegawa, Satomi Takahashi
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Publication number: 20040043460Abstract: The present invention relates to a method of producing an optically active pyridineethanol derivative. More particularly, it relates to a method of producing an optically active polycyclic pyridineethanol derivative by causing an enzyme or enzyme source to act on polycyclic acetylpyridine derivatives.Type: ApplicationFiled: September 25, 2003Publication date: March 4, 2004Applicant: Kaneka CorporationInventors: Shigeru Kawano, Miho Horikawa, Yoshihiko Yasohara, Junzo Hasegawa
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Patent number: 6645746Abstract: A novel polypeptide for producing tert-butyl (3R,5S)-6-chloro-3,5-dihydroxyhexanoate, a gene encoding the polypeptide, and a method of using the polypeptide, are provided. The polypeptide has an enzyme activity to asymmetrically reduce tert-butyl (S)-6-chloro-5-hydroxy-3-oxohexanoate to tert-butyl (3R,5S)-6-chloro-3,5-dihydroxyhexanoate. The polypeptide comprises the amino acid sequence represented by SEQ ID NO. 1 in the Sequence Listing, or the amino acid sequence having at least one amino acid substitution, insertion, deletion, or addition thereof.Type: GrantFiled: November 19, 2001Date of Patent: November 11, 2003Assignee: Kaneka CorporationInventors: Noriyuki Kizaki, Yukio Yamada, Yoshihiko Yasohara, Junzo Hasegawa
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Publication number: 20030186412Abstract: The present invention provides a novel polypeptide producing (S)-N-benzyl-3-pyrrolidinol, a DNA coding for it and a method of using them.Type: ApplicationFiled: June 3, 2002Publication date: October 2, 2003Inventors: Noriyuki Kizaki, Yoshihiko Yasohara, Junzo Hasegawa
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Publication number: 20020192786Abstract: To provide a method for preparing optically active compounds, which mainly contain (R)-amino compounds, by microbial enzymes efficiently and inexpensively; a polypeptide having stereoselective transaminase activity which can be suitably used for the above preparation method; and a DNA encoding the polypeptide. A method for preparing an optically active amino compound, characterized in stereoselectively transaminating by acting a transaminase on an amino group acceptor, a ketone compound in the presence of an amino group donor, a primary amine; a DNA comprising a nucleotide sequence encoding a polypeptide having stereoselective transaminase activity; and a polypeptide having stereoselective transaminase activity obtainable from a culture of a microorganism belonging to the genus Arthrobacter.Type: ApplicationFiled: April 2, 2002Publication date: December 19, 2002Applicant: Kaneka CorporationInventors: Yukio Yamada, Akira Iwasaki, Noriyuki Kizaki, Keiji Matsumoto, Yasuhiro Ikenaka, Masahiro Ogura, Junzo Hasegawa
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Patent number: 6461842Abstract: A gene of decaprenyl diphosphate synthase, which is the key gene participating in the biosynthesis of coenzyme Q10 was isolated from a bacterium belonging to the family Rhizobiaceae. By transferring this gene into a microorganism such as Escherichia coli and expressing therein, coenzyme Q10 can be effectively produced.Type: GrantFiled: October 10, 2000Date of Patent: October 8, 2002Assignee: Kaneka CorporationInventors: Hideyuki Matsuda, Makoto Kawamukai, Kazuyoshi Yajima, Yasuhiro Ikenaka, Kenichi Nishi, Junzo Hasegawa, Satomi Takahashi
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Patent number: 6448052Abstract: An enzyme having carbonyl reduction activity of reducing a carbonyl compound asymmetrically to produce an optically active alcohol, a DNA coding the enzyme, a plasmid having the DNA, a transformant which is a cell transformed with the plasmid, and a production method of an optically active alcohol using the enzyme and/or the transformed cell are provided.Type: GrantFiled: February 5, 2001Date of Patent: September 10, 2002Assignee: Kaneka CorporationInventors: Yoshihiko Yasohara, Noriyuki Kizaki, Junzo Hasegawa, Masaru Wada, Sakayu Shimizu, Michihiko Kataoka, Kazuhiko Yamamoto, Hiroshi Kawabata, Keiko Kita
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Patent number: 6410773Abstract: Novel and useful optical active 2-aralkyl-3-sulfonyloxy-1-propanol and 2-aralkyl-3-sulfonyloxypropionic acid are provided by using an optical active 2-aralkyl-3-acyloxy-1-propanol as a starting material. Furthermore, an optical active 2-aralkyl-3-thiopropionic acid, which is an important intermediate of enkephalinase inhibitor, is provided. According to the present invention, industrially useful optical active sulfonic acid ester derivatives can be provided.Type: GrantFiled: February 24, 2000Date of Patent: June 25, 2002Assignee: Kaneka CorporationInventors: Yoshihiko Yasohara, Kenji Miyamoto, Shigeru Kawano, Junzo Hasegawa
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Patent number: 6346402Abstract: Relating to an enzyme capable of efficiently converting a ketone compound to an optically active amino compound by transamination, and a process for preparing an optically active amino compound using the enzyme. An (S)-&agr;-phenethylamine:pyruvate transaminase, which acts on (S)-&agr;-phenethylamine and a ketone compound, thereby catalyzing transamination for forming acetophenone and an amino compound corresponding to the ketone compound; a process for preparing an optically active amino compound using the transaminase; and a method for culturing a microorganism for producing the above transaminase, comprising adding to a medium one or more compounds selected from the group consisting of propylamine, 1-butylamine, 2-butylamine, 2-pentylamine, isopropylamine and isobutylamine as an inducer for the enzyme when a microorganism for producing (S)-&agr;-phenethylamine:pyruvate transaminase is cultured.Type: GrantFiled: June 30, 2000Date of Patent: February 12, 2002Assignee: Kaneka CorporationInventors: Akira Iwasaki, Yukio Yamada, Yasuhiro Ikenaka, Yoshihiko Yasohara, Junzo Hasegawa
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Patent number: 6344351Abstract: The method for preparing an optically active (R)-amino compound characterized by the method comprising stereoselectively carrying out amino group transfer by action of an (R)-form-specific transaminase in the co-presence of a ketone compound (amino acceptor), and an amino compound (amino donor) of a racemic form or an (R)-form, to give an optically active (R)-amino compound. According to the present invention, it is made possible to easily prepare at a high yield the optically active (R)-amino compounds and the like having an aryl group and the like at their 1-position, which have been conventionally difficult to prepare.Type: GrantFiled: February 28, 2001Date of Patent: February 5, 2002Assignee: Kaneka CorporationInventors: Yukio Yamada, Akira Iwasaki, Noriyuki Kizaki, Yasuhiro Ikenaka, Masahiro Ogura, Junzo Hasegawa
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Publication number: 20020006651Abstract: An enzyme having carbonyl reduction activity of reducing a carbonyl compound asymmetrically to produce an optically active alcohol, a DNA coding the enzyme, a plasmid having the DNA, a transformant which is a cell transformed with the plasmid, and a production method of an optically active alcohol using the enzyme and/or the transformed cell are provided.Type: ApplicationFiled: February 5, 2001Publication date: January 17, 2002Applicant: KANEKA CORPORATIONInventors: Yoshihiko Yasohara, Noriyuki Kizaki, Junzo Hasegawa, Masaru Wada, Sakayu Shimizu, Michihiko Kataoka, Kazuhiko Yamamoto, Hiroshi Kawabata, Keiko Kita
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Publication number: 20010036660Abstract: A method of producing an optically active N-methylamino acid of the general formula (2): 1Type: ApplicationFiled: January 16, 2001Publication date: November 1, 2001Applicant: Kaneka CorporationInventors: Satoru Tsuda, Takahisa Kato, Yoshihiko Yasohara, Junzo Hasegawa
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Publication number: 20010031487Abstract: The method for preparing an optically active (R)-amino compound characterized by the method comprising stereoselectively carrying out amino group transfer by action of an (R)-form-specific transaminase in the co-presence of a ketone compound (amino acceptor), and an amino compound (amino donor) of a racemic form or an (R)-form, to give an optically active (R)-amino compound. According to the present invention, it is made possible to easily prepare at a high yield the optically active (R)-amino compounds and the like having an aryl group and the like at their 1-position, which have been conventionally difficult to prepare.Type: ApplicationFiled: February 28, 2001Publication date: October 18, 2001Applicant: Kaneka CorporationInventors: Yukio Yamada, Akira Iwasaki, Noriyuki Kizaki, Yasuhiro Ikenaka, Masahiro Ogura, Junzo Hasegawa
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Patent number: 6221638Abstract: The method for preparing an optically active (R)-amino compound characterized by the method comprising stereoselectively carrying out amino group transfer by action of an (R)-form-specific transaminase in the co-presence of a ketone compound (amino acceptor), and an amino compound (amino donor) of a racemic form or an (R)-form, to give an optically active (R)-amino compound. According to the present invention, it is made possible to easily prepare at a high yield the optically active (R)-amino compounds and the like having an aryl group and the like at their 1-position, which have been conventionally difficult to prepare.Type: GrantFiled: April 23, 1998Date of Patent: April 24, 2001Assignee: Kaneka CorporationInventors: Yukio Yamada, Akira Iwasaki, Noriyuki Kizaki, Yasuhiro Ikenaka, Masahiro Ogura, Junzo Hasegawa
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Patent number: 6218156Abstract: An enzyme having carbonyl reduction activity of reducing a carbonyl compound asymmetrically to produce an optically active alcohol, a DNA coding the enzyme, a plasmid having the DNA, a transformant which is a cell transformed with the plasmid, and a production method of an optically active alcohol using the enzyme and/or the transformed cell are provided.Type: GrantFiled: November 24, 1999Date of Patent: April 17, 2001Assignee: Kaneka CorporationInventors: Yoshihiko Yasohara, Noriyuki Kizaki, Junzo Hasegawa, Masaru Wada, Sakayu Shimizu, Michihiko Kataoka, Kazuhiko Yamamoto, Hiroshi Kawabata, Keiko Kita
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Patent number: 6214610Abstract: The invention provides an efficient method of producing optically active N-benzyl-3-pyrrolidinol by an enzymatic reaction stereoselectively reducing N-benzyl-3-pyrrolidinone. The invention consists in a method of producing optically active N-benzyl-3-pyrrolidinol which comprises a step of obtaining a reaction mixture by treating N-benzyl-3-pyrrolidinone with cells or a culture of a microorganism, or a material derived therefrom, and a step of recovering optically active N-benzyl-3-pyrrolidinol from said reaction mixture, in which method said microorganism mentioned above is a microorganism belonging to the genus Dipodascus, Debaryomyces, Cryptococcus, Pichia, Rhodosporidium, Trichosporon, Micrococcus, Komagataella, Ogataea or Zygosaccharomyces.Type: GrantFiled: June 28, 1999Date of Patent: April 10, 2001Assignee: Kaneka CorporationInventors: Yoshihiko Yasohara, Junzo Hasegawa
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Patent number: 6121477Abstract: Optically active 2-aralkyl-3-sulfonyloxy-1-propanol and 2-aralkyl-3-sulfonyloxypropionic acid are provided by using an optically active 2-aralkyl-3-acyloxy-1-propanol as a starting material. Furthermore, an optically active 2-aralkyl-3-thiopropionic acid, which is an important intermediate for synthesis of enkephalinase inhibitors, is provided. According to the present invention, industrially useful optically active sulfonic acid ester derivatives can be provided.Type: GrantFiled: January 29, 1999Date of Patent: September 19, 2000Assignee: Kaneka CorporationInventors: Yoshihiko Yasohara, Kenji Miyamoto, Shigeru Kawano, Junzo Hasegawa