Abstract: A method includes receiving, via at least one processor, data from a plurality of data sources disposed within an area, the data including area data, emergency event data, and emergency resource data; generating, via the at least one processor, based on the data, a visualization including a graphical representation of the area based on the area data, the emergency event data, the emergency resource data, or any combination thereof; determining, based on an interaction with the visualization, an emergency response plan for a vehicle, wherein the response plan includes an evacuation plan; sending the emergency response plan to a vehicle system associated with the vehicle, wherein the vehicle system is configured to: determine a location associated with the emergency response plan; determine a route to the location; and send instructions to one or more traffic assets along the route to the location, the instructions configured to cause the one or more traffic assets to adjust one or more signals.

Abstract: A method fabricating at least one universal substrate from a batch product. The method includes steps of: providing a preform having a predetermined profile; wrapping a plurality of conductors about an outer surface of the preform; injecting a nonconductive matrix between conductors of the plurality of conductors, wherein the nonconductive matrix permeates between interstitial spaces of the plurality of conductors to isolate some conductors of the plurality of conductors from one another; forming the batch product that includes the plurality of conductors and the nonconductive matrix; and wafering at least one section of the batch product to form the at least one universal substrate. The plurality of conductors of the at least one universal substrate defines a first connection surface, a second connection surface opposite to the first connection surface, and a plurality of conductive pathways defined between the first connection surface and the second connection surface.

Abstract: A transmission shift fork comprising: an arch-shaped portion, where a pair of lobes are radially and inwardly projecting from an inner surface of the arch-shaped portion directing oil to a sleeve engagement region of a clutch.

Abstract: An integrated circuit structure includes (i) a first layer including a first metal, (ii) a second layer above and in contact with the first layer, the second layer including a resistive material, and (iii) a third layer above and in contact with the second layer, the third layer including a second metal. In an example, the resistive material is different from one or both the first metal and the second metal. An interconnect component is above and in contact with the second layer. In an example, the interconnect component is a solder bump or a solder ball. In an example, a resistivity of the resistive material of the second layer is at least 20%, or at least 50% greater than a resistivity of each of the first and third layers. In an example, the resistive material includes a third metal different from the first and second metals and/or a metalloid.

Abstract: Described herein are methods for making genetically modified cells by introducing combinations of genetic variants (designed or random) or constructs (genes or otherwise arbitrary DNA) into a population of cells, and for tracking each variant combination by sequentially building an array of barcodes at a common locus (chromosomal or plasmid), termed the barcode locus. Also described are the cells made by such methods.

Abstract: The disclosure describes that both Cas9 and Cpf1 enzymes can exhibit potent nickase activities on an extensive class of mismatched dsDNA targets. These properties allow the production of efficient nickases for a chosen dsDNA target sequence, without modification of the nuclease protein, using guide RNAs with a variety of patterns of mismatch to the intended DNA target. In parallel to the nicking activities observed with purified Cas9 in vitro, sequence-dependent nicking for both perfectly matched and partially mismatched target sequences in a Saccharomyces cerevisae in vivo system was observed.

Abstract: The present disclosure relates to multiplex production and phenotyping of genetically engineered cells using RNA-guided nucleases and genomic barcoding. In particular, high-throughput multiplex genome editing is achieved utilizing a system that facilitates precise genome editing at desired target chromosomal loci by homology directed repair. Integration of guide RNA and donor DNA sequences as a genomic barcode at a separate chromosomal locus allows identification, isolation, and massively-parallel validation of individual variants from a pool of transformants. Strains can be arrayed according to their precise genetic modifications, as specified by donor DNA incorporation in heterologous or native genes.

Abstract: A jounce control damping system with an elongated housing containing a damping medium, a primary piston assembly, a moveable outer piston assembly, and a movable inner piston assembly disposed at least partially within the outer piston assembly. The primary piston assembly provides a first compression damping force. The inner piston engages the primary piston assembly and provides a second compression damping force when moved in a compression direction. After the inner piston assembly moves a given distance, the outer piston assembly is moved in the compressive direction and provides a third compression damping force.

Abstract: A jounce control damping system with an elongated housing containing a damping medium, a primary piston assembly, a moveable outer piston assembly, and a movable inner piston assembly disposed at least partially within the outer piston assembly. The primary piston assembly provides a first compression damping force. The inner piston engages the primary piston assembly and provides a second compression damping force when moved in a compression direction. After the inner piston assembly moves a given distance, the outer piston assembly is moved in the compressive direction and provides a third compression damping force.

Abstract: A jounce control damping system with an elongated housing containing a damping medium, a primary piston assembly, a moveable outer piston assembly, and a movable inner piston assembly disposed at least partially within the outer piston assembly. The primary piston assembly provides a first compression damping force. The inner piston engages the primary piston assembly and provides a second compression damping force when moved in a compression direction. After the inner piston assembly moves a given distance, the outer piston assembly is moved in the compressive direction and provides a third compression damping force.

Abstract: A jounce control damping system with an elongated housing containing a damping medium, a primary piston assembly, a moveable outer piston assembly, and a movable inner piston assembly disposed at least partially within the outer piston assembly. The primary piston assembly provides a first compression damping force. The inner piston engages the primary piston assembly and provides a second compression damping force when moved in a compression direction. After the inner piston assembly moves a given distance, the outer piston assembly is moved in the compressive direction and provides a third compression damping force.