Patents by Inventor Karsten Zengler

Karsten Zengler has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Publication number: 20210062171
    Abstract: The present disclosure relates to novel sialidase enzymes having a preference for cleaving N-glycolylneuraminic acid (Neu5Gc) from glycoconjugates over cleaving N-acetylneuraminic acid (Neu5Ac). The present disclosure further provides the polypeptide of such novel sialidase and its catalytic pockets; the recombinant host cells expressing the same, as well as the enzymatic, pharmaceutical, and consumable compositions comprising such novel sialidases. Probiotic compositions comprising a bacterium that expresses the sialidase having a preference for Neu5Gc over Neu5Ac, and methods for removing Neu5Gc from consumable products are also provided. Also disclosed are methods for treating or preventing various diseases, disorders, and inflammatory conditions in a subject using the novel sialidases.
    Type: Application
    Filed: August 28, 2020
    Publication date: March 4, 2021
    Applicant: The Regents of the University of California
    Inventors: Karsten Zengler, Ajit Varki, Frederico Da Silva, Sandra Diaz, Cameron Martino, Geoffrey Chang, Steven Rees, Livia Soares Zaramela
  • Patent number: 10900073
    Abstract: A microfabricated device defining a high density array of microwells is described for cultivating cells from a sample. A series of unique tags may be disposed in the microwells to identify one or more species of cells and locate the particular microwells in which each species was cultivated. A unique tag may be a nucleic acid molecule including a target-specific nucleotide sequence for annealing to a target nucleic acid fragment and a location-specific nucleotide sequence predetermined to identify one or more microwells. The device may be incubated to grow a plurality of cells, which may be split into an analysis portion and a reserve portion. High throughput methods are described for cultivating, screening, and determining a relative and/or absolute abundance of cells from a sample.
    Type: Grant
    Filed: April 21, 2016
    Date of Patent: January 26, 2021
    Assignee: GENERAL AUTOMATION LAB TECHNOLOGIES INC.
    Inventors: Paul C. Blainey, Michael W. Seely, Roman Stocker, Karsten Zengler, Scott Conradson, Peter Christey, Alexander Hallock
  • Publication number: 20190194736
    Abstract: A kit includes a microfabricated device having a top surface defining an array of microwells for receiving a sample comprising at least one cell, and a membrane for applying on the top surface of the microfabricated device to retain the at least one cell in at least one microwell of the array of microwells after the sample is loaded on the microfabricated device.
    Type: Application
    Filed: December 27, 2018
    Publication date: June 27, 2019
    Inventors: Paul C. Blainey, Michael W. Seely, Roman Stocker, Karsten Zengler, Scott Conradson, Peter Christey, Alexander Hallock
  • Publication number: 20190194735
    Abstract: A method of screening for at least one biological entity of interest using a microfabricated device which has a top surface defining an array of microwells. A sample is loaded onto the microfabricated device such that at least one microwell of the array of microwells includes at least one cell and an amount of a nutrient. A membrane is applied to the microfabricated device to retain the at least one cell and the nutrient. A plurality of cells is cultured from the at least one cell in the at least one microwell of the array of microwells. The plurality of cells in the at least one microwell of the array of microwells are split into a first portion of the plurality of cells and a second portion of the plurality of cells. The plurality of cells is analyzed to determine a presence or absence of a biological entity of interest.
    Type: Application
    Filed: December 27, 2018
    Publication date: June 27, 2019
    Inventors: Paul C. Blainey, Michael W. Seely, Roman Stocker, Karsten Zengler, Scott Conradson, Peter Christey, Alexander Hallock
  • Publication number: 20190194734
    Abstract: A method of screening for at least one biological entity of interest using a microfabricated device which has a top surface defining an array of microwells. A sample is loaded onto the microfabricated device such that at least one microwell of the array of microwells includes at least one cell and an amount of a nutrient. A membrane is applied to the microfabricated device to retain the at least one cell and the nutrient. A plurality of cells are cultured from the at least one cell in the at least one microwell of the array of microwells. The plurality of cells is then analyzed to determine a presence or absence of a biological entity of interest.
    Type: Application
    Filed: December 27, 2018
    Publication date: June 27, 2019
    Inventors: Paul C. Blainey, Michael W. Seely, Roman Stocker, Karsten Zengler, Scott Conradson, Peter Christey, Alexander Hallock
  • Patent number: 10227640
    Abstract: A method of screening for at least one biological entity of interest using a microfabricated device which has a top surface defining an array of microwells. A sample is loaded onto the microfabricated device such that at least one microwell of the array of microwells includes at least one cell and an amount of a nutrient. A membrane is applied to the microfabricated device to retain the at least one cell and the nutrient. Without furnishing additional nutrient to the microwells, the microfabricated device is incubated to grow a plurality of cells from the at least one cell in the at least one microwell of the array of microwells. The plurality of cells is then analyzed to determine a presence or absence of a biological entity of interest.
    Type: Grant
    Filed: September 30, 2017
    Date of Patent: March 12, 2019
    Assignee: GENERAL AUTOMATION LAB TECHNOLOGIES, INC.
    Inventors: Paul C. Blainey, Michael W. Seely, Roman Stocker, Karsten Zengler, Scott Conradson, Peter Christey, Alexander Hallock
  • Publication number: 20180051327
    Abstract: A method of screening for at least one biological entity of interest using a microfabricated device which has a top surface defining an array of microwells. A sample is loaded onto the microfabricated device such that at least one microwell of the array of microwells includes at least one cell and an amount of a nutrient. A membrane is applied to the microfabricated device to retain the at least one cell and the nutrient. Without furnishing additional nutrient to the microwells, the microfabricated device is incubated to grow a plurality of cells from the at least one cell in the at least one microwell of the array of microwells. The plurality of cells is then analyzed to determine a presence or absence of a biological entity of interest.
    Type: Application
    Filed: September 30, 2017
    Publication date: February 22, 2018
    Inventors: Paul C. Blainey, Michael W. Seely, Roman Stocker, Karsten Zengler, Scott Conradson, Peter Christey, Alexander Hallock
  • Publication number: 20180023045
    Abstract: A method for selecting a medium is provided. The method includes obtaining a microfabricated device including a plurality of microwells; loading a plurality of different media into the plurality of microwells such that each microwell of the plurality comprises a medium and the plurality of microwells comprises a plurality of different media; loading at least one cell from a sample into each microwell of the plurality microwells; incubating the microfabricated device at a predetermined condition for a predetermined duration of time; comparing the contents of the plurality of microwells across the plurality of microwells; and based on the comparison, determining at least one medium out of the plurality of different media.
    Type: Application
    Filed: June 27, 2017
    Publication date: January 25, 2018
    Inventors: Alexander Hallock, Karsten Zengler, Peter Christey
  • Publication number: 20170342457
    Abstract: Methods, apparatuses, and systems for screening, analyzing and selecting microorganisms from complex heterogeneous communities, predicting and identifying functional relationships and interactions thereof, and synthesizing microbial ensembles based thereon are disclosed. Methods for identifying and determining the absolute cell count of microorganism types and strains, along with identifying the network relationships between active microorganisms and environmental parameters, are also disclosed.
    Type: Application
    Filed: November 11, 2016
    Publication date: November 30, 2017
    Applicant: Ascus Biosciences, Inc.
    Inventors: Mallory EMBREE, Karsten ZENGLER
  • Publication number: 20170107557
    Abstract: Methods, apparatuses, and systems for microorganism strain analysis of complex heterogeneous communities, predicting and identifying functional relationships and interactions thereof, and selecting and synthesizing microbial ensembles based thereon are disclosed. Methods for identifying and determining the absolute cell count of microorganism types and strains, along with identifying the network relationships between active microorganisms and metadata, are also disclosed.
    Type: Application
    Filed: December 28, 2016
    Publication date: April 20, 2017
    Applicant: Ascus Biosciences, Inc.
    Inventors: Mallory EMBREE, Karsten ZENGLER
  • Patent number: 9540676
    Abstract: Methods, apparatuses, and systems for screening, analyzing and selecting microorganisms from complex heterogeneous communities, predicting and identifying functional relationships and interactions thereof, and synthesizing microbial ensembles based thereon are disclosed. Methods for identifying and determining the absolute cell count of microorganism types and strains, along with identifying the network relationships between active microorganisms and environmental parameters, are also disclosed.
    Type: Grant
    Filed: July 22, 2016
    Date of Patent: January 10, 2017
    Assignee: Ascus Biosciences, Inc.
    Inventors: Karsten Zengler, Mallory Embree
  • Publication number: 20160376627
    Abstract: Methods, apparatuses, and systems for screening, analyzing and selecting microorganisms from complex heterogeneous communities, predicting and identifying functional relationships and interactions thereof, and synthesizing microbial ensembles based thereon are disclosed. Methods for identifying and determining the absolute cell count of microorganism types and strains, along with identifying the network relationships between active microorganisms and environmental parameters, are also disclosed.
    Type: Application
    Filed: July 22, 2016
    Publication date: December 29, 2016
    Inventors: Karsten ZENGLER, Mallory EMBREE
  • Publication number: 20160312275
    Abstract: A microfabricated device defining a high density array of microwells is described for cultivating cells from a sample. A series of unique tags may be disposed in the microwells to identify one or more species of cells and locate the particular microwells in which each species was cultivated. A unique tag may be a nucleic acid molecule including a target-specific nucleotide sequence for annealing to a target nucleic acid fragment and a location-specific nucleotide sequence predetermined to identify one or more microwells. The device may be incubated to grow a plurality of cells, which may be split into an analysis portion and a reserve portion. High throughput methods are described for cultivating, screening, and determining a relative and/or absolute abundance of cells from a sample.
    Type: Application
    Filed: April 21, 2016
    Publication date: October 27, 2016
    Inventors: Paul C. Blainey, Michael W. Seely, Roman Stocker, Karsten Zengler, Scott Conradson, Peter Christey, Alexander Hallock