Patents by Inventor Kim J. Stutzman-Engwall

Kim J. Stutzman-Engwall has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • gene directing the ratio of B2:B1 avermectins
    Patent number: 8008078
    Abstract: The present invention relates to polynucleotide molecules comprising nucleotide sequences encoding an aveC gene product, which polynucleotide molecules can be used to alter the ratio or amount of class 2:1 avermectins produced in fermentation cultures of S. avermitilis. The present invention further relates to vectors, host cells, and mutant strains of S. avermitilis in which the aveC gene has been inactivated, or mutated so as to change the ratio or amount of class 2:1 avermectins produced.
    Type: Grant
    Filed: April 23, 2008
    Date of Patent: August 30, 2011
    Assignees: Pfizer Inc., Pfizer Products Inc.
    Inventors: Kim J. Stutzman-Engwall, Anke Krebber, Claes Gustafsson, Jeremy S. Minshull, Sun Ai Raillard, Seran Kim, Yan Chen
  • Polynucleotides and methods of directing the ratio of B2:B1 avermectins in host cells
    Patent number: 7858340
    Abstract: The present invention relates to polynucleotide molecules comprising nucleotide sequences encoding an aveC gene product, which polynucleotide molecules can be used to alter the ratio or amount of class 2:1 avermectins produced in fermentation cultures of S. avermitilis. The present invention further relates to vectors, host cells, and mutant strains of S. avermitilis in which the aveC gene has been inactivated, or mutated so as to change the ratio or amount of class 2:1 avermectins produced.
    Type: Grant
    Filed: June 11, 2003
    Date of Patent: December 28, 2010
    Assignees: Pfizer Inc., Pfizer Products Inc.
    Inventors: Kim J. Stutzman-Engwall, Yan Chen, Claes Gustafsson, Anke Krebber, Jeremy Minshull, Sun Ai Raillard
  • Streptomyces Avermitilis Gene Directing The Ratio of B2:B1 Avermectins
    Publication number: 20090017508
    Abstract: The present invention relates to polynucleotide molecules comprising nucleotide sequences encoding an aveC gene product, which polynucleotide molecules can be used to alter the ratio or amount of class 2:1 avermectins produced in fermentation cultures of S. avermitilis. The present invention further relates to vectors, host cells, and mutant strains of S. avermitilis in which the aveC gene has been inactivated, or mutated so as to change the ratio or amount of class 2:1 avermectins produced.
    Type: Application
    Filed: April 23, 2008
    Publication date: January 15, 2009
    Inventors: Kim J. Stutzman-Engwall, Anke Krebber, Claes Gustafsson, Jeremy S. Minshull, Sun Ai Raillard, Seran Kim, Yan Chen
  • gene directing the ratio of B2:B1 avermectins
    Patent number: 7388085
    Abstract: The present invention relates to polynucleotide molecules comprising nucleotide sequences encoding an aveC gene product, which polynucleotide molecules can be used to alter the ratio or amount of class 2:1 avermectins produced in fermentation cultures of S. avermitilis. The present invention further relates to vectors, host cells, and mutant strains of S. avermitilis in which the aveC gene has been inactivated, or mutated so as to change the ratio or amount of class 2:1 avermectins produced.
    Type: Grant
    Filed: February 10, 2003
    Date of Patent: June 17, 2008
    Assignee: Pfizer Inc
    Inventors: Kim J. Stutzman-Engwall, Anke Krebber, Claes Gustafsson, Jeremy S. Minshull, Sun Ai Raillard, Seran Kim, Yan Chen
  • gene directing the ratio of B2:B1 avermectins
    Patent number: 7259241
    Abstract: The present invention relates to polynucleotide molecules comprising nucleotide sequences encoding an aveC gene product, which polynucleotide molecules can be used to alter the ratio or amount of class 2:1 avermectins produced in fermentation cultures of S. avermitilis. The present invention further relates to vectors, host cells, and mutant strains of S. avermitilis in which the aveC gene has been inactivated, or mutated so as to change the ratio or amount of class 2:1 avermectins produced.
    Type: Grant
    Filed: December 14, 2004
    Date of Patent: August 21, 2007
    Assignees: Pfizer Inc., Pfizer Products Inc.
    Inventors: Kim J. Stutzman-Engwall, Hamish McArthur
  • Methods related to gene directing the ratio of B2:B1 avermectins
    Patent number: 7029887
    Abstract: The present invention relates to polynucleotide molecules comprising sequences encoding avec gene products, which polynucleotide molecules can be used to alter the ratio or amount of class 2:1 avermectins produced in fermentation cultures of Streptomyces avermitilis. AveC genes, homologs and partial homologs thereof are described for S. avermitilis, S. hygroscopicus, and S. griseochromogenes, respectively. The present invention further relates to vectors, host cells, and mutant strains of Streptomyces avermitilis in which the avec gene has been inactivated, or mutated so as to change the ratio or amount of class 2:1 avermectins produced.
    Type: Grant
    Filed: November 27, 2002
    Date of Patent: April 18, 2006
    Assignee: Pfizer Inc.
    Inventors: Kim J. Stutzman-Engwall, Hamish McArthur, Yoshihiro Katoh
  • AveC gene product from Streptomyces hygroscopicus
    Patent number: 6833263
    Abstract: The present invention relates to polynucleotide molecules comprising sequences encoding avec gene products, which polynucleotide molecules can be used to alter the ratio or amount of class 2:1 avermectins produced in fermentation cultures of Streptomyces avermitilis. AveC genes, homologs and partial homologs thereof are described for S. avermitilis , S. hygroscopicus, and S. griseochromogenes, respectively. The present invention further relates to vectors, host cells, and mutant strains of Streptomyces avermitilis in which the avec gene has been inactivated, or mutated so as to change the ratio or amount of class 2:1 avermectins produced.
    Type: Grant
    Filed: November 27, 2002
    Date of Patent: December 21, 2004
    Assignee: Pfizer Inc.
    Inventors: Kim J. Stutzman-Engwall, Hamish McArthur, Yoshihiro Katoh
  • Modified Streptomyces host cells for increased avermectin production and methods of making the same
    Patent number: 6689611
    Abstract: The present invention is directed to compositions and methods for producing avermectins, and is primarily in the field of animal health. The present invention relates to the identification and characterization of two novel genes, herein referred to as the aveR1 and aveR2 genes, that are involved in regulating avermectin polyketide synthase (PKS) expression and avermectin biosynthesis in Streptomyces avermitilis. The present invention is based on the discovery that inactivation of these genes results in an increase in the amount of avermectin produced by S. avermitilis.
    Type: Grant
    Filed: November 16, 2000
    Date of Patent: February 10, 2004
    Assignee: Pfizer Inc.
    Inventors: Kim J. Stutzman-Engwall, Brenda S. Price
  • Directing the ration of B2:B1 avermectins in Streptomyces avermitilis host cells
    Publication number: 20040009560
    Abstract: The present invention relates to polynucleotide molecules comprising nucleotide sequences encoding an aveC gene product, which polynucleotide molecules can be used to alter the ratio or amount of class 2:1 avermectins produced in fermentation cultures of S. avermitilis. The present invention further relates to vectors, host cells, and mutant strains of S. avermitilis in which the aveC gene has been inactivated, or mutated so as to change the ratio or amount of class 2:1 avermectins produced.
    Type: Application
    Filed: June 11, 2003
    Publication date: January 15, 2004
    Applicant: Pfizer Inc.
    Inventors: Kim J. Stutzman-Engwall, Chen Yan, Claes Gustafsson, Anke Krebber, Jeremy Minshull, Sun Ai Raillard
  • Directing the ration of B2:B1 avermectins in Streptomyces avermitilis host cells
    Publication number: 20030232415
    Abstract: The present invention relates to polynucleotide molecules comprising nucleotide sequences encoding an aveC gene product, which polynucleotide molecules can be used to alter the ratio or amount of class 2:1 avermectins produced in fermentation cultures of S. avermitilis. The present invention further relates to vectors, host cells, and mutant strains of S. avermitilis in which the aveC gene has been inactivated, or mutated so as to change the ratio or amount of class 2:1 avermectins produced.
    Type: Application
    Filed: June 11, 2003
    Publication date: December 18, 2003
    Applicant: Pfizer Inc.
    Inventors: Kim J. Stutzman-Engwall, Yan Chen, Claes Gustafsson, Anke Krebber, Jeremy Minshull, Sun Ai Raillard
  • Directing the ratio of B2:B1 avermectins in Streptomyces avermitilis host cells
    Patent number: 6632673
    Abstract: The present invention relates to polynucleotide molecules comprising nucleotide sequences encoding an aveC gene product, which polynucleotide molecules can be used to alter the ratio or amount of class 2:1 avermectins produced in fermentation cultures of S. avermitilis. The present invention further relates to vectors, host cells, and mutant strains of S. avermitilis in which the aveC gene has been inactivated, or mutated so as to change the ratio or amount of class 2:1 avermectins produced.
    Type: Grant
    Filed: August 10, 2000
    Date of Patent: October 14, 2003
    Assignee: Pfizer, Inc.
    Inventors: Kim J. Stutzman-Engwall, Yan Chen, Claes Gustafsson, Anke Krebber, Jeremy Minshull, Sun Ai Raillard
  • Streptomyces avermitilis gene directing the ratio of B2:B1 avermectins
    Publication number: 20030166165
    Abstract: The present invention relates to polynucleotide molecules comprising nucleotide sequences encoding an aveC gene product, which polynucleotide molecules can be used to alter the ratio or amount of class 2:1 avermectins produced in fermentation cultures of S. avermitilis. The present invention further relates to vectors, host cells, and mutant strains of S. avermitilis in which the aveC gene has been inactivated, or mutated so as to change the ratio or amount of class 2:1 avermectins produced.
    Type: Application
    Filed: February 10, 2003
    Publication date: September 4, 2003
    Inventors: Kim J. Stutzman-Engwall, Anke Krebber, Claes Gustafsson, Jeremy S. Minshull, Sun Ai Raillard, Seran Kim, Yan Chen
  • Streptomyces avermitilis gene directing the ratio of B2:B1 avermectins
    Publication number: 20030134313
    Abstract: The present invention relates to polynucleotide molecules comprising nucleotide sequences encoding an aveC gene product, which polynucleotide molecules can be used to alter the ratio or amount of class 2:1 avermectins produced in fermentation cultures of S. avermitilis. The present invention further relates to vectors, host cells, and mutant strains of S. avermitilis in which the aveC gene has been inactivated, or mutated so as to change the ratio or amount of class 2:1 avermectins produced.
    Type: Application
    Filed: November 27, 2002
    Publication date: July 17, 2003
    Inventors: Kim J. Stutzman-Engwall, Hamish McArthur, Yoshihiro Katoh
  • Streptomyces avermitilis gene directing the ratio of B2:B1 avermectins
    Publication number: 20030129636
    Abstract: The present invention relates to polynucleotide molecules comprising nucleotide sequences encoding an aveC gene product, which polynucleotide molecules can be used to alter the ratio or amount of class 2:1 avermectins produced in fermentation cultures of S. avermitilis. The present invention further relates to vectors, host cells, and mutant strains of S. avermitilis in which the aveC gene has been inactivated, or mutated so as to change the ratio or amount of class 2:1 avermectins produced.
    Type: Application
    Filed: November 27, 2002
    Publication date: July 10, 2003
    Inventors: Kim J. Stutzman-Engwall, Hamish McArthur, Yoshihiro Katoh
  • Compositions related to Streptomyces avermitilis gene directing the ratio of B2:B1 avermectins
    Publication number: 20030129635
    Abstract: The present invention relates to polynucleotide molecules comprising nucleotide sequences encoding an aveC gene product, which polynucleotide molecules can be used to alter the ratio or amount of class 2:1 avermectins produced in fermentation cultures of S. avermitilis. The present invention further relates to vectors, host cells, and mutant strains of S. avermitilis in which the aveC gene has been inactivated, or mutated so as to change the ratio or amount of class 2:1 avermectins produced.
    Type: Application
    Filed: November 27, 2002
    Publication date: July 10, 2003
    Inventors: Kim J. Stutzman-Engwall, Hamish McArthur, Yoshihiro Katoh
  • Methods related to streptomyces avermitilis gene directing the ratio of B2:B1 avermectins
    Publication number: 20030124607
    Abstract: The present invention relates to polynucleotide molecules comprising nucleotide sequences encoding an aveC gene product, which polynucleotide molecules can be used to alter the ratio or amount of class 2:1 avermectins produced in fermentation cultures of S. avermitilis. The present invention further relates to vectors, host cells, and mutant strains of S. avermitilis in which the aveC gene has been inactivated, or mutated so as to change the ratio or amount of class 2:1 avermectins produced.
    Type: Application
    Filed: November 27, 2002
    Publication date: July 3, 2003
    Inventors: Kim J. Stutzman-Engwall, Hamish McArthur, Yoshihiro Katoh
  • Streptomyces avermitilis gene directing the ratio of B2:B1 avermectins
    Patent number: 6511841
    Abstract: The present invention relates to polynucleotide molecules comprising nucleotide sequences encoding an aveC gene product, which polynucleotide molecules can be used to alter the ratio or amount of class 2:1 avermectins produced in fermentation cultures of S. avermitilis. The present invention further relates to vectors, host cells, and mutant strains of S. avermitilis in which the aveC gene has been inactivated, or mutated so as to change the ratio or amount of class 2:1 avermectins produced.
    Type: Grant
    Filed: January 22, 2001
    Date of Patent: January 28, 2003
    Assignees: Pfizer, Inc., Pfizer Products, Inc.
    Inventors: Kim J. Stutzman-Engwall, Hamish McArthur, Yoshihiro Katoh
  • Methods related to Streptomyces avermitilis gene directing the ratio of B2:B1 avermectins
    Patent number: 6509159
    Abstract: The present invention relates to polynucleotide molecules comprising nucleotide sequences encoding the aveC gene product, which polynucleotide molecules can be used to alter the ratio or amount of class 2:1 avermectins produced in fermentation cultures of Streptomyces avermitilis. The present invention further relates to vectors, host cells, and mutant strains of Streptomyces avermitilis in which the aveC gene has been inactivated, or mutated so as to change the ratio or amount of class 2:1 avermectins produced.
    Type: Grant
    Filed: January 22, 2001
    Date of Patent: January 21, 2003
    Assignees: Pfizer Inc., Pfizer Products Inc.
    Inventors: Kim J. Stutzman-Engwall, Hamish McArthur, Yoshihiro Katoh
  • Genes encoding branched-chain alpha-ketoacid dehydrogenase complex from Streptomyces avermitilis
    Patent number: 6399324
    Abstract: The present invention relates to novel DNA sequences that encode for the branched-chain alpha-ketoacid dehydrogenase complex of an organism belonging to the genus Streptomyces and to novel polypeptides produced by the expression of such sequences. It also relates to novel methods of enhancing the production of natural avermectin, of producing a Streptomyces avermitilis bkd mutant and of producing novel avermectins through fermentation.
    Type: Grant
    Filed: June 7, 1995
    Date of Patent: June 4, 2002
    Assignee: Pfizer Inc.
    Inventors: Claudio D. Denoya, Kim J. Stutzman-Engwall
  • Streptomyces avermitilis gene directing the ratio of B2:B1 avermectins
    Publication number: 20010024818
    Abstract: The present invention relates to polynucleotide molecules comprising nucleotide sequences encoding an aveC gene product, which polynucleotide molecules can be used to alter the ratio or amount of class 2:1 avermectins produced in fermentation cultures of S. avermitilis. The present invention further relates to vectors, host cells, and mutant strains of S. avermitilis in which the aveC gene has been inactivated, or mutated so as to change the ratio or amount of class 2:1 avermectins produced.
    Type: Application
    Filed: January 22, 2001
    Publication date: September 27, 2001
    Inventors: Kim J. Stutzman-Engwall, Hamish McArthur, Yoshihiro Katoh