Patents by Inventor Kimiyasu Isobe
Kimiyasu Isobe has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Publication number: 20180105840Abstract: The present invention provides a process for industrial production of chiral-1,1-difluoro-2-propanol. More specifically, a microorganism having the activity to cause asymmetric reduction of 1,1-difluoroacetone or an enzyme having the same activity is allowed to act on 1,1-difluoroacetone, whereby chiral-1,1-difluoro-2-propanol can be produced with high optical purity and in good yield. The process for production of the present invention is easy for industrial implementation.Type: ApplicationFiled: March 2, 2016Publication date: April 19, 2018Applicants: PUBLIC UNIVERSITY CORPORATION TOYAMA PREFECTURAL UNIVERSITY, CENTRAL GLASS COMPANY, LIMITEDInventors: Yasuhisa ASANO, Kimiyasu ISOBE, Ryoko UEDA, Tetsuro NISHII, Akihiro ISHII
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Publication number: 20120149071Abstract: A D-aminoacylase having a high substrate specificity is provided. This D-aminoacylase can produce D-amino acids from N-acetyl-D,L-amino acids conveniently and efficiently at a low cost. A D-aminoacylase produced by a microorganism of genus Defluvibacter; which acts on a N-acetyl-D-amino acid; which has a molecular weight (as determined by electrophoresis) of about 55,000 daltons, and an isoelectric point (as determined by two-dimensional electrophoresis for denatured system) of 5.3; which acts on N-acetyl-D-valine, N-acetyl-D-leucine, and the like, but not on N-acetyl-L-valine, N-acetyl-L-leucine, and the like; which has an optimal temperature of 37° C. (pH 8) and an optimal pH value of 8 to 8.5 at 37° C.; and whose activity is inhibited by Mn2+, Co2+, Ni2+, and Zn2+ each at 1 mmol/L, and by dithiothreitol, 2-mercaptoethanol, o-phenanthroline, and L-cysteine each at 5 mmol/L.Type: ApplicationFiled: January 6, 2012Publication date: June 14, 2012Applicants: ISOBE Kimiyasu, DAIICHI PURE CHEMICALS CO., LTD.Inventors: Kimiyasu Isobe, Seiki Yamaguchi
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Publication number: 20090215118Abstract: A D-aminoacylase having a high substrate specificity is provided. This D-aminoacylase can produce D-amino acids from N-acetyl-D,L-amino acids conveniently and efficiently at a low cost. A D-aminoacylase produced by a microorganism of genus Defluvibacter; which acts on a N-acetyl-D-amino acid; which has a molecular weight (as determined by electrophoresis) of about 55,000 daltons, and an isoelectric point (as determined by two-dimensional electrophoresis for denatured system) of 5.3; which acts on N-acetyl-D-valine, N-acetyl-D-leucine, and the like, but not on N-acetyl-L-valine, N-acetyl-L-leucine, and the like; which has an optimal temperature of 37° C. (pH 8) and an optimal pH value of 8 to 8.5 at 37° C.; and whose activity is inhibited by Mn2+, Co2+, Ni2+, and Zn2+ each at 1 mmol/L, and by dithiothreitol, 2-mercaptoethanol, o-phenanthroline, and L-cysteine each at 5 mmol/L.Type: ApplicationFiled: March 4, 2009Publication date: August 27, 2009Applicants: DAIICHI PURE CHEMICALS CO., LTD., ISOBE KimiyasuInventors: Kimiyasu ISOBE, Seiki Yamaguchi, Masayuki Kobayashi, Shinya Kumagai, Takami Sarashina
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Publication number: 20080096244Abstract: A D-aminoacylase having a high substrate specificity is provided. This D-aminoacylase can produce D-amino acids from N-acetyl-D,L-amino acids conveniently and efficiently at a low cost. A D-aminoacylase produced by a microorganism of genus Defluvibacter; which acts on a N-acetyl-D-amino acid; which has a molecular weight (as determined by electrophoresis) of about 55,000 daltons, and an isoelectric point (as determined by two-dimensional electrophoresis for denatured system) of 5.3; which acts on N-acetyl-D-valine, N-acetyl-D-leucine, and the like, but not on N-acetyl-L-valine, N-acetyl-L-leucine, and the like; which has an optimal temperature of 37° C. (pH 8) and an optimal pH value of 8 to 8.5 at 37° C.; and whose activity is inhibited by Mn2+, Co2+, Ni2+, and Zn2+ each at 1 mmol/L, and by dithiothreitol, 2-mercaptoethanol, o-phenanthroline, and L-cysteine each at 5 mmol/L.Type: ApplicationFiled: January 25, 2007Publication date: April 24, 2008Applicants: DAIICHI PURE CHEMICALS CO., LTD., ISOBE KimiyasuInventors: Kimiyasu Isobe, Seiki Yamaguchi, Masayuki Kobayashi, Shinya Kumagai, Takami Sarashina
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Publication number: 20060172375Abstract: A D-aminoacylase having a high substrate specificity is provided. This D-aminoacylase can produce D-amino acids from N-acetyl-D,L-amino acids conveniently and efficiently at a low cost. A D-aminoacylase produced by a microorganism of genus Defluvibacter; which acts on a N-acetyl-D-amino acid; which has a molecular weight (as determined by electrophoresis) of about 55,000 daltons, and an isoelectric point (as determined by two-dimensional electrophoresis for denatured system) of 5.3; which acts on N-acetyl-D-valine, N-acetyl-D-leucine, and the like, but not on N-acetyl-L-valine, N-acetyl-L-leucine, and the like; which has an optimal temperature of 37° C. (pH 8) and an optimal pH value of 8 to 8.5 at 37° C.; and whose activity is inhibited by Mn2+, Co2+, Ni2+, and Zn2+ each at 1 mmol/L, and by dithiothreitol, 2-mercaptoethanol, o-phenanthroline, and L-cysteine each at 5 mmol/L.Type: ApplicationFiled: December 17, 2003Publication date: August 3, 2006Applicants: DAIICHI PURE CHEMICALS CO., LTD, ISOBE KIMIYASUInventors: Kimiyasu Isobe, Seiki Yamaguchi, Masayuki Kobayashi, Shinya Kumagai, Takami Sarashina
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Patent number: 6943004Abstract: A transformed microorganism prepared by inserting into a host microorganism with zinc tolerance a D-aminoacylase-producing gene which selectively produces D-aminoacylase alone between D-aminoacylase and L-aminoacylase. A process comprising culturing the transformed microorganism in a culture medium containing zinc ion and obtaining D-aminoacylase from the culture at a high efficiency.Type: GrantFiled: June 15, 2000Date of Patent: September 13, 2005Assignee: Amano Enzyme Inc.Inventors: Ken-ichi Takeuchi, Yoshinao Koide, Yoshihiko Hirose, Mitsuaki Moriguchi, Kimiyasu Isobe
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Patent number: 4999289Abstract: Lipase isolated from Penicillium characterized by the following properties:(1) Efficiently hydrolyzes triglycerides of fatty acids having 4-18 carbon atoms;(2) Hydrolysis of such triglycerides with such lipase produces at least 5 moles of glycerol per 100 moles of fatty acid;(3) Optimal pH range for said hydrolysis 5-7;(4) Stable in the pH range of 4.5-6;(5) Optimal temperature range for activity 35.degree.-40.degree. C.;(6) thermal stability up to about 35.degree. C.;(7) Activated by surface-active agents, but not substantially inhibited by such surface-active agents in concentration up to 5%;(8) Molecular weight about 1000,000 to 120,000;(9) Isoelectric point about pH 3.84.Type: GrantFiled: April 6, 1987Date of Patent: March 12, 1991Assignee: Amano Pharmaceutical Co. Ltd.,Inventors: Tetsunori Akiba, Shotaro Yamaguchi, Satoru Suzuki, Kimiyasu Isobe, Kuniyoshi Matsunaga
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Patent number: 4617263Abstract: A rapid and reliable method is proposed for the quantitative determination of the individual free and acetylated polyamines, i.e. spermine, spermidine, putrescine, acetylspermine, N.sup.1 - and N.sup.8 -acetylspermidines and acetylputrescine in the body fluid of a patient suffering cancer or other diseases to facilitate diagnosis and therapeutic treatment. The method comprises subjecting the aqueous fluid to several enzymatic oxidative decomposition reactions of the polyamines in sequence or in combination by use of the 4 enzymes, i.e. polyamine oxidase AT-1, polyamine oxidase PC-3, putrescine oxidase and acetylputrescine oxidase, and determining, preferably photometrically, the amount of hydrogen peroxide formed as a byproduct in each of the reaction steps followed by setting up a set of simultaneous equations to give the respective amounts of the individual polyamines as a function of the amounts of the hydrogen peroxide formed in the reaction steps and solving the simultaneous equations.Type: GrantFiled: September 13, 1985Date of Patent: October 14, 1986Assignee: Amano Seiyaku Kabushiki KaishaInventors: Hideaki Yamada, Shogo Otsuji, Kimiyasu Isobe, Kuniyoshi Matsunaga, Tadahiko Inukai
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Patent number: 4550078Abstract: A rapid and reliable method is proposed for the quantitative determination of the individual free and acetylated polyamines, i.e. spermine, spermidine, putrescine, acetylspermine, N.sup.1 - and N.sup.8 -acetylspermidines and acetylputrescine in the body fluid of a patient suffering cancer or other deseases to facilitate diagnosis and therapeutic treatment. The method comprises subjecting the aqueous fluid to several enzymatic oxidative decomposition reactions of the polyamines in sequence or in combination by use of the 4 enzymes, i.e. polyamine oxidase AT-1, polyamine oxidase PC-3, putrescine oxidase and acetylputrescine oxidase, and determining, preferably photometrically, the amount of hydrogen peroxide formed as a byproduct in each of the reaction steps followed by setting up a set of simultaneous equations to give the respective amounts of the individual polyamines as a function of the amounts of the hydrogen peroxide formed in the reaction steps and solving the simultaneous equations.Type: GrantFiled: May 18, 1983Date of Patent: October 29, 1985Assignee: Amano Seiyaku Kabushiki KaishaInventors: Hideaki Yamada, Shogo Otsuji, Kimiyasu Isobe, Kuniyoshi Matsunaga, Tadahiko Inukai
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Patent number: 4373026Abstract: A novel polyamine oxidase, polyamine oxidase PC-3, takes an important role participating in decomposition and metabolism and polyamines such as spermidine and spermine.Type: GrantFiled: December 12, 1980Date of Patent: February 8, 1983Assignee: Amano Pharmaceutical Co., Ltd.Inventors: Hideaki Yamada, Yoshiki Tani, Kimiyasu Isobe
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Patent number: 4371621Abstract: A novel polyamine oxidase, polyamine oxidase AT-1, takes an important role participating in decomposition and metabolism of polyamines such as spermidine and spermine.Type: GrantFiled: December 12, 1980Date of Patent: February 1, 1983Assignee: Amano Pharmaceutical Co., Ltd.Inventors: Hideaki Yamada, Yoshiki Tani, Kimiyasu Isobe