Patents by Inventor Kiwamu Imagawa
Kiwamu Imagawa has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Publication number: 20230086254Abstract: To provide a pharmaceutical composition having a novel use comprising a dental pulp-derived multipotent stem cell preparation that can be administered to humans. A pharmaceutical composition comprising dental pulp-derived stem cells as an active ingredient for suppressing infiltration into a tissue of at lease neutrophils, monocytes, or lymphocytes.Type: ApplicationFiled: January 29, 2021Publication date: March 23, 2023Inventors: Kiwamu IMAGAWA, Kohtaro MINAMI, Kenichi MAEDA, Yuki HOSODA, Shunsuke WATANABE, Kazutoshi SATO, Yasuna HIGASHIGUCHI, Takashi KUSHIDA, Ayumi ISHIWARI, Yu TSUSHIMA, Yoshiteru MIYAUCHI
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Publication number: 20210387119Abstract: A filtration filter is configured from a first weld frame, a second weld frame, and a filter sandwiched and welded between the first weld frame and the second weld frame. The first weld frame and the second weld frame are configured by a flexible film having a film thickness of at least 120 ?m. The filter is configured by a substance having a higher melting point than the first weld frame and the second weld frame and having an opening area ratio of from 10% to 80%. The first weld frame is configured by a polymer that includes either a high density polyethylene having a melting point of from 120° C. to 140° C., a linear low density polyethylene having a melting point of from 105° C. to 125° C., or a mixture including at least one of the high density polyethylene or the linear low density polyethylene.Type: ApplicationFiled: October 21, 2019Publication date: December 16, 2021Applicants: TEIJIN LIMITED, JCR PHARMACEUTICALS CO., LTD.Inventors: Azusa KOHNO, Takashi KUSHIDA, Ayumi ISHIWARI, Kiwamu IMAGAWA, Yuki HOSODA
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Publication number: 20210301258Abstract: The present disclosure relates to a method for producing dental pulp-derived cells enriched with pluripotent stem cells including: (a) digesting dental pulp with a protease to prepare a dental pulp suspension; (b) culturing the suspension to proliferate pluripotent stem cells contained in the suspension; (c) freezing the proliferated pluripotent stem cells in a state in which the pluripotent stem cells are suspended in a first cryopreservation liquid; (d) thawing the frozen pluripotent stem cells; (e) culturing the thawed pluripotent stem cells in a state in which the pluripotent stem cells are adhered to surfaces of particles to proliferate the pluripotent stem cells on the surfaces of the particles; and (f) bringing the particles into contact with a protease to separate the pluripotent stem cells adhered to the surfaces of the particles from the particles.Type: ApplicationFiled: July 30, 2019Publication date: September 30, 2021Applicants: JCR Pharmaceuticals Co., Ltd., Teijin LimitedInventors: Kiwamu Imagawa, Kotaro Minami, Kenichi Maeda, Yuki Hosoda, Shunsuke Watanabe, Kazutoshi Sato, Yasuna Higashiguchi, Takashi Kushida, Ayumi Ishiwari
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Patent number: 10335439Abstract: [Problem] To provide a method for producing human corneal epithelial sheet, wherein the human corneal epithelial-derived cells obtained by culturing human corneal epithelial cells are cultured on an amnion substrate. [Solution] A method for culturing human corneal epithelial cells using mesenchymal stem cells as the feeder cells; and a method for culturing human corneal epithelial cells using a medium containing a ROCK inhibitor, a phosphodiesterase inhibitor, a MAP kinase inhibitor and a TGF-? receptor inhibitor in various combinations.Type: GrantFiled: March 9, 2014Date of Patent: July 2, 2019Assignees: JCR Pharmaceuticals Co., Ltd., Kyoto Prefectural Public University CorporationInventors: Kiwamu Imagawa, Kenichi Maeda, Yuki Hosoda, Shuichi Yokoyama, Naoki Okumura, Noriko Koizumi, Shigeru Kinoshita
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Patent number: 10174284Abstract: [Problem] To provide, in order to manufacture cells to transplant into patients with corneal endothelial failure, a medium used to culture corneal endothelial cells obtained from human corneal tissue and grow said cells while maintaining the morphology thereof as corneal endothelial cells. [Solution] A medium containing a conditioned medium from mesenchymal stem cells; and a method in which said medium is used to culture corneal endothelial cells.Type: GrantFiled: September 5, 2013Date of Patent: January 8, 2019Assignees: JCR PHARMACEUTICALS CO., LTD., THE DOSHISHA, KYOTO PREFECTURAL PUBLIC UNIVERSITY CORPORATIONInventors: Michio Hagiya, Kiwamu Imagawa, Yuuki Hosoda, Noriko Koizumi, Naoki Okumura, Makiko Nakahara, Shigeru Kinoshita
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Patent number: 10041039Abstract: Disclosed is a method for producing pluripotent stem cell-enriched human dental pulp-derived cells. The method is characterized in that it includes (a) culturing a dental pulp suspension in a feeder cells-culture vessel containing feeder cells whose proliferative ability is suppressed, on a membrane having micropores that can block passage of the feeder cells and supported in the feeder cells-culture vessel in a manner to prevent the lower side face thereof from contacting with the feeder cells, thereby preventing direct contact with the feeder cells, and, (b) a step for recovering the cells proliferating on the membrane.Type: GrantFiled: March 28, 2013Date of Patent: August 7, 2018Assignee: JCR PHARMACEUTICALS CO., LTD.Inventor: Kiwamu Imagawa
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Publication number: 20170327791Abstract: Disclosed are a therapeutic agent for muscular dystrophy employing pluripotent stem cells obtained from dental pulp and a method for preparation thereof. The therapeutic agent for muscular dystrophy comprises pluripotent stem cell-enriched human dental pulp-derived cells as the active ingredient, and is prepared by a method of preparation comprising the steps of: (a) adding dental pulp-derived cells contained in a dental pulp suspension, in a culture vessel containing feeder cells whose proliferative ability is suppressed, onto a membrane having micropores that can block feeder cells from passing therethrough and supported within the vessel in a manner that avoids direct contact of the lower side face thereof with the feeder cells, and culturing the dental pulp-derived cells on the membrane while preventing direct contact with the feeder cells, and (b) recovering the cells having grown on the membrane as the pluripotent stem cell-enriched human dental pulp-derived cells.Type: ApplicationFiled: November 13, 2015Publication date: November 16, 2017Inventors: Takashi OKADA, Yuko KASAHARA, Kiwamu IMAGAWA
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Publication number: 20160008408Abstract: [Problem] To provide a method for producing human corneal epithelial sheet, wherein the human corneal epithelial-derived cells obtained by culturing human corneal epithelial cells are cultured on an amnion substrate. [Solution] A method for culturing human corneal epithelial cells using mesenchymal stem cells as the feeder cells; and a method for culturing human corneal epithelial cells using a medium containing a ROCK inhibitor, a phosphodiesterase inhibitor, a MAP kinase inhibitor and a TGF-? receptor inhibitor in various combinations.Type: ApplicationFiled: March 9, 2014Publication date: January 14, 2016Applicants: JCR PHARMACEUTICALS CO., LTD., KYOTO PREFECTURAL PUBLIC UNIVERSITY CORPORATIONInventors: Kiwamu IMAGAWA, Kenichi MAEDA, Yuki HOSODA, Shuichi YOKOYAMA, Naoki OKUMURA, Noriko KOIZUMI, Shigeru KINOSHITA
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Publication number: 20150210981Abstract: [Problem] To provide, in order to manufacture cells to transplant into patients with corneal endothelial failure, a medium used to culture corneal endothelial cells obtained from human corneal tissue and grow said cells while maintaining the morphology thereof as corneal endothelial cells. [Solution] A medium containing a conditioned medium from mesenchymal stem cells; and a method in which said medium is used to culture corneal endothelial cells.Type: ApplicationFiled: September 5, 2013Publication date: July 30, 2015Inventors: Michio Hagiya, Kiwamu Imagawa, Yuuki Hosoda, Noriko Koizumi, Naoki Okumura, Makiko Nakahara, Shigeru kinoshita
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Publication number: 20150050731Abstract: Disclosed is a method for producing pluripotent stem cell-enriched human dental pulp-derived cells. The method is characterized in that it includes (a) culturing a dental pulp suspension in a feeder cells-culture vessel containing feeder cells whose proliferative ability is suppressed, on a membrane having micropores that can block passage of the feeder cells and supported in the feeder cells-culture vessel in a manner to prevent the lower side face thereof from contacting with the feeder cells, thereby preventing direct contact with the feeder cells, and, (b) a step for recovering the cells proliferating on the membrane.Type: ApplicationFiled: March 28, 2013Publication date: February 19, 2015Inventor: Kiwamu Imagawa
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Patent number: 8465733Abstract: Disclosed are a method for producing a frozen pharmaceutical composition prepared from cultured human mesenchymal stem cells and a pharmaceutical composition containing human mesenchymal stem cells. This method is a method for producing a frozen pharmaceutical composition containing human mesenchymal stem cells, which comprises the following steps in this order: (a) adding trypsin to human mesenchymal stem cells in a culture vessel to detach the cells from the surface of the culture vessel; (b) adding a bicarbonate Ringer's solution containing human serum albumin to the detached cells to terminate the reaction with trypsin, and washing the cells with the bicarbonate Ringer's solution containing human serum albumin; (c) suspending the cells in a bicarbonate Ringer's solution containing human serum albumin and dimethyl sulfoxide; (d) putting the resulting suspension in a container which allows freezing of what is contained therein, and sealing the container; and (e) freezing the suspension put in the container.Type: GrantFiled: October 27, 2008Date of Patent: June 18, 2013Assignee: JCR Pharmaceuticals Co., Ltd.Inventors: Hiroyuki Shirono, Youngjin Byun, Shoichiro Kamei, Kiwamu Imagawa
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Publication number: 20110274663Abstract: Disclosed are a method for producing a frozen pharmaceutical composition prepared from cultured human mesenchymal stem cells and a pharmaceutical composition containing human mesenchymal stem cells. This method is a method for producing a frozen pharmaceutical composition containing human mesenchymal stem cells, which comprises the following steps in this order: (a) adding trypsin to human mesenchymal stem cells in a culture vessel to detach the cells from the surface of the culture vessel; (b) adding a bicarbonate Ringer's solution containing human serum albumin to the detached cells to terminate the reaction with trypsin, and washing the cells with the bicarbonate Ringer's solution containing human serum albumin; (c) suspending the cells in a bicarbonate Ringer's solution containing human serum albumin and dimethyl sulfoxide; (d) putting the resulting suspension in a container which allows freezing of what is contained therein, and sealing the container; and (e) freezing the suspension put in the container.Type: ApplicationFiled: October 27, 2008Publication date: November 10, 2011Applicant: JCR Pharmaceuticals Co., Ltd.Inventors: Hiroyuki Shirono, Youngjin Byun, Shoichiro Kamei, Kiwamu Imagawa