Patents by Inventor Kiyozo Asada
Kiyozo Asada has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Patent number: 9670478Abstract: The present invention pertains to a method for modifying a nucleic acid contained in a sample comprising a step for bringing the sample into contact with a nucleic acid-modifying agent in the presence of an acidic polysaccharide and/or a nucleotide; and a method for selectively detecting a nucleic acid derived from living cells contained in the sample, comprising the following steps: (a) a step for modifying a nucleic acid contained in a sample according to the method for modifying a nucleic acid contained in a sample, which includes a step for bringing the sample into contact with a nucleic acid-modifying agent in the presence of an acidic polysaccharide and/or a nucleotide; and (b) a step for selectively detecting an unmodified nucleic acid from the sample after step (a). The present invention further pertains to a kit and composition for use in these methods.Type: GrantFiled: August 31, 2016Date of Patent: June 6, 2017Assignee: TAKARA BIO INC.Inventors: Junko Yamamoto, Keiko Kubo, Takashi Uemori, Hiroyuki Mukai, Kiyozo Asada
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Publication number: 20160362676Abstract: The present invention pertains to: a method for modifying a nucleic acid contained in a sample, said method including a step for bringing the sample into contact with a nucleic acid-modifying agent in the presence of an acidic polysaccharide and/or a nucleotide; and a method for selectively detecting a nucleic acid derived from living cells contained in the sample, said method including the following steps: (a) a step for modifying a nucleic acid contained in a sample according to the method for modifying a nucleic acid contained in a sample, which includes a step for bringing the sample into contact with a nucleic acid-modifying agent in the presence of an acidic polysaccharide and/or a nucleotide; and (b) a step for selectively detecting an unmodified nucleic acid from the sample after step (a). The present invention further pertains to a kit and composition for use in these methods.Type: ApplicationFiled: August 31, 2016Publication date: December 15, 2016Inventors: Junko YAMAMOTO, Keiko KUBO, Takashi UEMORI, Hiroyuki MUKAI, Kiyozo ASADA
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Patent number: 9458498Abstract: The present invention pertains to: a method for modifying a nucleic acid contained in a sample, the method including a step for bringing the sample into contact with a nucleic acid-modifying agent in the presence of an acidic polysaccharide and/or a nucleotide; and a method for selectively detecting a nucleic acid derived from living cells contained in the sample, the method including the following steps: (a) a step for modifying a nucleic acid contained in a sample according to the method for modifying a nucleic acid contained in a sample, which includes a step for bringing the sample into contact with a nucleic acid-modifying agent in the presence of an acidic polysaccharide and/or a nucleotide; and (b) a step for selectively detecting an unmodified nucleic acid from the sample after step (a). The present invention further pertains to a kit and composition for use in these methods.Type: GrantFiled: January 20, 2012Date of Patent: October 4, 2016Assignee: TAKARA BIO INC.Inventors: Junko Yamamoto, Keiko Kubo, Takashi Uemori, Hiroyuki Mukai, Kiyozo Asada
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Patent number: 9336349Abstract: An object of the present invention is to provide a simple and useful method for producing an RNA-containing probe for detecting a target nucleotide, a simple and useful method, device, and system for processing nucleotide sequence information, and a simple and useful method for detecting a target nucleotide. The present invention provides a method for processing nucleotide sequence information, the method comprising the step of generating partial nucleotide sequences which has 7 to 14 nucleotides and a Tm value of 25 to 40° C. and in which a target nucleotide or a nucleotide adjacent to the target nucleotide is located at a position between 3 and 5 nucleotides from the 3? or 5? end. The method according to the present invention is useful for simply and efficiently producing an RNA-containing probe for detecting a target nucleic acid, without the basis of researchers' experiences or guess, and are extremely useful not only in the field of genetic engineering, but also in the field of medical research.Type: GrantFiled: July 28, 2011Date of Patent: May 10, 2016Assignee: Takara Bio Inc.Inventors: Junko Yamamoto, Tomoo Inden, Toshiharu Ohba, Tooru Suzuki, Hiroyuki Mukai, Kiyozo Asada
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Patent number: 9169482Abstract: A method for synthesizing cDNA characterized by preparing a reaction solution that does not allow an endodeoxyribonuclease to show its activity, without thermal deactivation of the endodeoxyribonuclease or removal of the endodeoxyribonuclease, and carrying out a reverse transcription reaction, wherein the reaction solution contains a treated sample and a reverse transcriptase, the treated sample being formed by treating a sample comprising RNA and DNA with the endodeoxyribonuclease to degrade DNA in the sample. The method and the kit for synthesizing cDNA of the present invention are widely useful in genetic engineering fields.Type: GrantFiled: May 9, 2011Date of Patent: October 27, 2015Assignee: TAKARA BIO INC.Inventors: Yuko Nakabayashi, Takashi Uemori, Hiroyuki Mukai, Kiyozo Asada
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Patent number: 8852915Abstract: A method of retrovirus storage, characterized in that a retrovirus is sustained in the presence of a substance with retrovirus binding activity immobilized on a solid phase. Further, there is provided a retrovirus composition characterized in that a retrovirus in the form of binding to a substance with retrovirus binding activity is sealed in a container holding a solid phase having the substance with retrovirus binding activity immobilized thereon.Type: GrantFiled: October 12, 2012Date of Patent: October 7, 2014Assignee: Takara Bio Inc.Inventors: Hideto Chono, Yasushi Katayama, Hiromi Okuyama, Junichi Mineno, Kiyozo Asada, Ikunoshin Kato
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Publication number: 20130295576Abstract: The present invention pertains to: a method for modifying a nucleic acid contained in a sample, said method including a step for bringing the sample into contact with a nucleic acid-modifying agent in the presence of an acidic polysaccharide and/or a nucleotide; and a method for selectively detecting a nucleic acid derived from living cells contained in the sample, said method including the following steps: (a) a step for modifying a nucleic acid contained in a sample according to the method for modifying a nucleic acid contained in a sample, which includes a step for bringing the sample into contact with a nucleic acid-modifying agent in the presence of an acidic polysaccharide and/or a nucleotide; and (b) a step for selectively detecting an unmodified nucleic acid from the sample after step (a). The present invention further pertains to a kit and composition for use in these methods.Type: ApplicationFiled: January 20, 2012Publication date: November 7, 2013Inventors: Junko Yamamoto, Keiko Kubo, Takashi Uemori, Hiroyuki Mukai, Kiyozo Asada
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Publication number: 20130122503Abstract: An object of the present invention is to provide a simple and useful method for producing an RNA-containing probe for detecting a target nucleotide, a simple and useful method, device, and system for processing nucleotide sequence information, and a simple and useful method for detecting a target nucleotide. The present invention provides a method for processing nucleotide sequence information, the method comprising the step of generating partial nucleotide sequences which has 7 to 14 nucleotides and a Tm value of 25 to 40° C. and in which a target nucleotide or a nucleotide adjacent to the target nucleotide is located at a position between 3 and 5 nucleotides from the 3? or 5? end. The method according to the present invention is useful for simply and efficiently producing an RNA-containing probe for detecting a target nucleic acid, without the basis of researchers' experiences or guess, and are extremely useful not only in the field of genetic engineering, but also in the field of medical research.Type: ApplicationFiled: July 28, 2011Publication date: May 16, 2013Inventors: Junko Yamamoto, Tomoo Inden, Toshiharu Ohba, Tooru Suzuki, Hiroyuki Mukai, Kiyozo Asada
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Publication number: 20130065281Abstract: A method for synthesizing cDNA characterized by preparing a reaction solution that does not allow an endodeoxyribonuclease to show its activity, without thermal deactivation of the endodeoxyribonuclease or removal of the endodeoxyribonuclease, and carrying out a reverse transcription reaction, wherein the reaction solution contains a treated sample and a reverse transcriptase, the treated sample being formed by treating a sample comprising RNA and DNA with the endodeoxyribonuclease to degrade DNA in the sample. The method and the kit for synthesizing cDNA of the present invention are widely useful in genetic engineering fields.Type: ApplicationFiled: May 9, 2011Publication date: March 14, 2013Applicant: TAKARA BIO INC.Inventors: Yuko Nakabayashi, Takashi Uemori, Hiroyuki Mukai, Kiyozo Asada
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Patent number: 8367328Abstract: A DNA synthesis reaction-enhancer comprising at least one kind selected from the group consisting of acidic substances and cationic complexes; a DNA synthesis method in which during a DNA synthesis reaction a reaction is carried out in the presence of the above enhancer by using DNA polymerase; a DNA synthesis reaction composition comprising the above enhancer; a DNA synthesis reaction composition comprising two or more kinds of DNA polymerases each having 3??5? exonuclease activity; a DNA synthesis method in which during a DNA synthesis reaction two or more kinds of DNA polymerases each having 3??5? exonuclease activity are used; a kit for use in in vitro DNA synthesis, comprising two or more kinds of DNA polymerases each having 3??5? exonuclease activity; and a kit for use in in vitro DNA synthesis, wherein the kit comprises the DNA synthesis reaction-enhancer and DNA polymerase.Type: GrantFiled: October 15, 2008Date of Patent: February 5, 2013Assignee: Takara Bio Inc.Inventors: Kiyozo Asada, Takashi Uemori, Yoshimi Sato, Tomoko Fujita, Kazue Miyake, Osamu Takeda, Hiroyuki Mukai, Ikunoshin Kato
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Patent number: 8017356Abstract: A polypeptide having a endoribonuclease activity; a nucleic acid encoding the polypeptide; recombinant DNA having the nucleic acid therein; a transformant transformed with the recombinant DNA; a process for producing the polypeptide comprising the steps of cultivating the transformant and collecting the polypeptide from the culture; a process for producing a digest of single-stranded RNA comprising the step of reacting the polypeptide with the single-stranded RNA; and a method for the digestion of single-stranded RNA.Type: GrantFiled: July 4, 2006Date of Patent: September 13, 2011Assignee: Takara Bio Inc.Inventors: Masamitsu Shimada, Masanori Takayama, Kiyozo Asada, Ikunoshin Kato
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Patent number: 7989184Abstract: A polypeptide having a novel endoribonuclease activity; a nucleic acid encoding the polypeptide; recombinant DNA having the nucleic acid therein; a transformant transformed with the recombinant DNA; a process for producing the polypeptide comprising the steps of cultivating the transformant and collecting the polypeptide from the culture; a process for producing a digest of single-stranded RNA comprising the step of reacting the polypeptide with the single-stranded RNA; and a method for the digestion of single-stranded RNA.Type: GrantFiled: July 4, 2006Date of Patent: August 2, 2011Assignee: Takara Bio Inc.Inventors: Masamitsu Shimada, Masanori Takayama, Kiyozo Asada, Ikunoshin Kato
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Patent number: 7790849Abstract: An isolated polypeptide includes the amino acid sequence shown in SEQ. ID No. 13 and an isolated nucleic acid encoding the polypeptide, such as an isolated nucleic acid including the nucleic acid sequence shown in SEQ. ID No. 17. The isolated polypeptide includes two heparin binding polypeptides connected in tandem and the isolated nucleic acid encodes these. The polypeptide and nucleic acid sequences can improve retroviral vector mediated gene transfer efficiency into target cells.Type: GrantFiled: September 30, 2008Date of Patent: September 7, 2010Assignee: Takara Bio Inc.Inventors: Kiyozo Asada, Takashi Uemori, Takashi Ueno, Nobuto Koyama, Kimikazu Hashino, Ikunoshin Kato
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Publication number: 20100093026Abstract: A polypeptide having a novel endoribonuclease activity; a nucleic acid encoding the polypeptide; recombinant DNA having the nucleic acid therein; a transformant transformed with the recombinant DNA; a process for producing the polypeptide comprising the steps of cultivating the transformant and collecting the polypeptide from the culture; a process for producing a digest of single-stranded RNA comprising the step of reacting the polypeptide with the single-stranded RNA; and a method for the digestion of single-stranded RNA.Type: ApplicationFiled: July 4, 2006Publication date: April 15, 2010Inventors: Masamitsu Shimada, Masanori Takayama, Kiyozo Asada, Ikunoshin Kato
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Publication number: 20090209730Abstract: A polypeptide represented by SEQ. ID No. 13 and a gene encoding the polypeptide. A gene is also represented by SEQ. ID No. 17, or a gene hybridizable thereto under stringent conditions and encoding a polypeptide which improves the efficiency of gene transfer into target cells with a retrovirus.Type: ApplicationFiled: September 30, 2008Publication date: August 20, 2009Inventors: Kiyozo Asada, Takashi Uemori, Takashi Ueno, Nobuto Koyama, Kimikazu Hashino, Ikunoshin Kato
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Patent number: 7553946Abstract: Isolated DNAs which are DNAs having a base sequence represented by any of SEQ ID NOS:1 to 6 in Sequence Listing or fragments thereof and showing a stationary phase-specific promoter activity in gram positive bacteria.Type: GrantFiled: August 8, 2006Date of Patent: June 30, 2009Assignee: Takara Bio Inc.Inventors: Tomoko Shimojo, Hikaru Takakura, Kazuyori Ochiai, Kiyozo Asada, Ikunoshin Kato
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Publication number: 20090156420Abstract: To provide a method for selecting a marker gene useful for cancer classification; a method for classifying cancer using the gene; a method for detecting cancer; a kit usable for the classification method or detection method; and a DNA array carrying the gene. According to the present invention, there can be obtained a gene, wherein expression of the above gene is altered independently from genes each of which expression is altered specifically during cell proliferation and expression level of the above gene is specifically altered depending on every type of cancer samples to be tested, whereby the classification or detection of cancer can be carried out conveniently and quickly without giving surgical treatment. Therefore, the present invention is useful for the diagnosis, the treatment, and the like of cancer.Type: ApplicationFiled: August 25, 2008Publication date: June 18, 2009Inventors: Yasuhiro Tsubosa, Kazuhiko Aoyagi, Hiroki Sasaki, Masaaki Terada, Junichi Mineno, Kiyozo Asada, Ikunoshin Kato
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Patent number: 7521178Abstract: A DNA synthesis reaction-enhancer comprising at least one kind selected from the group consisting of acidic substances and cationic complexes; a DNA synthesis method in which during a DNA synthesis reaction a reaction is carried out in the presence of the above enhancer by using DNA polymerase; a DNA synthesis reaction composition comprising the above enhancer; a DNA synthesis reaction composition comprising two or more kinds of DNA polymerases each having 3??5? exonuclease activity; a DNA synthesis method in which during a DNA synthesis reaction two or more kinds of DNA polymerases each having 3??5? exonuclease activity are used; a kit for use in in vitro DNA synthesis, comprising two or more kinds of DNA polymerases each having 3??5? exonuclease activity; and a kit for use in in vitro DNA synthesis, wherein the kit comprises the DNA synthesis reaction-enhancer and DNA polymerase.Type: GrantFiled: April 21, 1999Date of Patent: April 21, 2009Assignee: Takara Bio Inc.Inventors: Kiyozo Asada, Takashi Uemori, Yoshimi Sato, Tomoko Fujita, Kazue Miyake, Osamu Takeda, Hiroyuki Mukai, Ikunoshin Kato
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Publication number: 20090098613Abstract: A DNA synthesis reaction-enhancer comprising at least one kind selected from the group consisting of acidic substances and cationic complexes; a DNA synthesis method in which during a DNA synthesis reaction a reaction is carried out in the presence of the above enhancer by using DNA polymerase; a DNA synthesis reaction composition comprising the above enhancer; a DNA synthesis reaction composition comprising two or more kinds of DNA polymerases each having 3??5? exonuclease activity; a DNA synthesis method in which during a DNA synthesis reaction two or more kinds of DNA polymerases each having 3??5? exonuclease activity are used; a kit for use in in vitro DNA synthesis, comprising two or more kinds of DNA polymerases each having 3??5? exonuclease activity; and a kit for use in in vitro DNA synthesis, wherein the kit comprises the DNA synthesis reaction-enhancer and DNA polymerase.Type: ApplicationFiled: October 15, 2008Publication date: April 16, 2009Inventors: Kiyozo Asada, Takashi Uemori, Yoshimi Sato, Tomoko Fujita, Kazue Miyake, Osamu Takeda, Hiroyuki Mukai, Ikunoshin Kato
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Publication number: 20090047709Abstract: A novel endoribonuclease activity exhibiting polypeptide; a nucleic acid coding for the polypeptide; a recombinant DNA comprising the nucleic acid; a transformant obtained by transformation using the recombinant DNA; a process for producing the polypeptide, characterized by including the steps of culturing the transformant and collecting the polypeptide from the culture; a process for producing single-strand RNA fragments, characterized by including the step of causing the polypeptide to act on a single-strand RNA; and a method of fragmenting a single-strand RNA.Type: ApplicationFiled: September 8, 2006Publication date: February 19, 2009Applicant: TAKARA BIO INC.Inventors: Masamitsu Shimada, Masanori Takayama, Kiyozo Asada, Ikunoshin Kato