Abstract: An injection molded article with which a sheet material is integrated is manufactured without causing damage to the sheet material. A molten resin injected into a mold cavity, passes smoothly a peripheral edge of the mold cavity, and flows into a groove portion without accumulating in the vicinity of the peripheral edge. When cooling the molten resin, the temperature of the molten resin in the groove decreases more quickly than the temperature of the molten resin in the mold cavity, because the volume of the groove portion is small. The viscosity of the molten resin is increased rapidly, preventing further molten resin from flowing into the groove portion. The sheet material in the vicinity of the peripheral edge is covered and protected against exposure to heat and/or pressure of the molten resin.

Abstract: An injection molded article with which a sheet-like material, such as a decorative sheet, is integrated is manufactured without causing damage to the sheet-like material. A molten resin injected into a mold cavity, passes smoothly a peripheral edge of the mold cavity, and flows into a groove portion without accumulating in the vicinity of the peripheral edge. When cooling the molten resin, the temperature of the molten resin in the groove decreases more quickly than the temperature of the molten resin in the mold cavity, because the volume of the groove portion is smaller. As a result, the viscosity of the molten resin in the groove portion and in the vicinity of the peripheral edge of the mold cavity is increased rapidly, preventing a further volume of the molten resin from flowing into the groove portion. The sheet-like material in the vicinity of the peripheral edge is covered by the resinous material of diminished fluidity and protected against exposure to heat and/or pressure of the molten resin.

Abstract: An injection molded article with which a sheet-like material, such as a decorative sheet, is integrated is manufactured without causing damage to the sheet-like material. A molten resin injected into a mold cavity, passes smoothly a peripheral edge of the mold cavity, and flows into a groove portion without accumulating in the vicinity of the peripheral edge. When cooling the molten resin, the temperature of the molten resin in the groove decreases more quickly than the temperature of the molten resin in the mold cavity, because the volume of the groove portion is smaller. As a result, the viscosity of the molten resin in the groove portion and in the vicinity of the peripheral edge of the mold cavity is increased rapidly, preventing a further volume of the molten resin from flowing into the groove portion. The sheet-like material in the vicinity of the peripheral edge is covered by the resinous material of diminished fluidity and protected against exposure to heat and/or pressure of the molten resin.

Abstract: According to the present invention, a method to protect a transgene from silencing, wherein an insulator from sea urchin arylsulfatase gene is introduced concurrently with the transgene so as to provide a novel method for stabilization of a viral vector expression, is provided. Furthermore, according to this invention, a method for introducing a gene, wherein an insulator from sea urchin arylsulfatase gene is introduced concurrently with a transgene so as to protect the transgene from silencing, is provided. Furthermore, according to this invention, a method for production of a vector, wherein an insulator from sea urchin arylsulfatase gene is introduced into the vector so as to protect the vector from silencing, is provided. Because the viral vector containing an insulator from sea urchin arylsulfatase gene can protect a transgene from silencing, a novel useful method for gene therapy is provided.

Abstract: A method for the stable expression of an introduced exogenous gene in a plant or plant cell is provided. Stable expression of an exogenous gene that was introduced was achieved by operably linking an upstream sequence of sea urchin arylsulfatase gene as an insulator.

Abstract: There is provided a method of introducing exogenous genes into cultured cells or fertilized eggs, characterized in that each exogenous gene is introduced into chromosomal DNA in a cultured cell or fertilized egg under a condition where the exogenous gene is placed between insulators so as to ensure an insulated environment, such that the introduced exogenous gene will not be affected by any influence from adjacent genes in the cultured cell or fertilized egg.