Abstract: The exosome analysis method of the present invention comprises (a) bringing an exosome-containing sample into contact with a substrate that is modified with a compound having a hydrophobic chain and a hydrophilic chain to bind the exosome to the compound; (b) bringing the exosome into contact with a first molecule that specifically binds to a biomolecule existing on the surface of the exosome to form a first molecule-exosome complex on the substrate; and (c) detecting the first molecule-exosome complex on the substrate.

Abstract: A valve is provided with a tubular structure having an outer tube and an inner tube, and a diaphragm member having a thin film disposed to cover one end of the inner tube and an anchor part which encircles a peripheral edge of the thin film and comes into close contact with an inner wall of the outer tube and an outer wall of the inner tube.

Abstract: A microparticle detection system includes a stage unit including a mounting surface on which a fluid device having a flow path through which a sample containing microparticles is movable is capable of being mounted, an emission unit configured to emit illumination light to the flow path, an imaging unit configured to image scattered light generated from microparticles in the sample when illumination light is emitted, an identification unit configured to identify the microparticles included in the image for each of the microparticles on the basis of the image captured by the imaging unit, a particle size determination unit configured to determine a particle size of the microparticle for each of the microparticles identified by the identification unit, a zeta potential determination unit configured to determine a zeta potential of the microparticle for each of the microparticles identified by the identification unit, and a correlation unit configured to associate the particle size for each of the microparticles

Abstract: A particle detection method in which particles in a sample are detected includes: a mounting step of mounting, on a stage portion, a fluid device including a channel through which the particles can move; an irradiation step of irradiating the channel with illumination light; and a detection step of detecting scattered light generated from the particles by irradiation with the illumination light. In the irradiation step, the illumination light is converged such as to enter the channel by passing through, among side surfaces of the channel, only the first side surface that faces an illumination light incident direction.

Abstract: A valve disposed at a flow path includes: a substrate having a first surface in which a hole having an opening section is formed; and a diaphragm member fixed to at least part of a wall surface of the hole and in which at least a central portion has a thin film shape, wherein a flow of a fluid in the flow path is controlled by deforming the diaphragm member.

Abstract: A particle analysis apparatus includes: an acquisition unit that acquires a plurality of images each captured at a different time in each of which a particle moving in a predetermined direction in a medium is imaged; and a determination unit that determines, based on a movement amount of a particle due to Brownian motion in the medium, whether or not an image of a first particle included in an image captured at a first time of the plurality of images acquired by the acquisition unit and an image of a second particle included in an image captured at a second time which is different from the first time of the plurality of images acquired by the acquisition unit are images indicating the same particle.

Abstract: A microparticle detection system includes a stage unit including a mounting surface on which a fluid device having a flow path through which a sample containing microparticles is movable is capable of being mounted, an emission unit configured to emit illumination light to the flow path, an imaging unit configured to image scattered light generated from microparticles in the sample when illumination light is emitted, an identification unit configured to identify the microparticles included in the image for each of the microparticles on the basis of the image captured by the imaging unit, a particle size determination unit configured to determine a particle size of the microparticle for each of the microparticles identified by the identification unit, a zeta potential determination unit configured to determine a zeta potential of the microparticle for each of the microparticles identified by the identification unit, and a correlation unit configured to associate the particle size for each of the microparticles

Abstract: A particle detection method in which particles in a sample are detected includes: a mounting step of mounting, on a stage portion, a fluid device including a channel through which the particles can move; an irradiation step of irradiating the channel with illumination light; and a detection step of detecting scattered light generated from the particles by irradiation with the illumination light. In the irradiation step, the illumination light is converged such as to enter the channel by passing through, among side surfaces of the channel, only the first side surface that faces an illumination light incident direction.

Abstract: A valve is provided with a tubular structure having an outer tube and an inner tube, and a diaphragm member having a thin film disposed to cover one end of the inner tube and an anchor part which encircles a peripheral edge of the thin film and comes into close contact with an inner wall of the outer tube and an outer wall of the inner tube.

Abstract: A method for detecting a nucleic acid includes (a) bringing a solution including a nucleic acid and a detection probe labeled with a labeling substance into contact with a solid phase on which a capture probe is immobilized, the capture probe having a first portion including a sequence capable of hybridizing with the nucleic acid, a second portion including a sequence capable of hybridizing with the detection probe, and a stem portion for forming a double strand; (b) hybridizing the nucleic acid with the first portion, hybridizing the detection probe with the second portion, and forming a nucleic acid-detection probe-capture probe complex on the solid phase; (c) ligating the nucleic acid and the detection probe; (d) eliminating binding between the capture probe and the detection probe that has not been ligated with the nucleic acid; and (e) detecting the labeling substance in the complex.

Abstract: A particle analysis apparatus includes: an acquisition unit that acquires a plurality of images each captured at a different time in each of which a particle moving in a predetermined direction in a medium is imaged; and a determination unit that determines, based on a movement amount of a particle due to Brownian motion in the medium, whether or not an image of a first particle included in an image captured at a first time of the plurality of images acquired by the acquisition unit and an image of a second particle included in an image captured at a second time which is different from the first time of the plurality of images acquired by the acquisition unit are images indicating the same particle.

Abstract: A valve disposed at a flow path includes: a substrate having a first surface in which a hole having an opening section is formed; and a diaphragm member fixed to at least part of a wall surface of the hole and in which at least a central portion has a thin film shape, wherein a flow of a fluid in the flow path is controlled by deforming the diaphragm member.

Abstract: The present invention provides a separation unit, a separation method, a fluid device, a composite fluid device and kit which are able to obtain a target analyte from a sample including the analyte. The separation unit of the present invention is characterized in being provided with a filter for selectively filtering analyte from a sample, and a structural material arranged to be movable to the secondary side of the filter so as to promote filtration while maintaining contact with the analyte filtrated by the filter.

Abstract: The exosome analysis method of the present invention comprises (a) bringing an exosome-containing sample into contact with a substrate that is modified with a compound having a hydrophobic chain and a hydrophilic chain to bind the exosome to the compound; (b) bringing the exosome into contact with a first molecule that specifically binds to a biomolecule existing on the surface of the exosome to form a first molecule-exosome complex on the substrate; and (c) detecting the first molecule-exosome complex on the substrate.