Patents by Inventor Larry B. Tsai

Larry B. Tsai has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Enhanced secretion of polypeptides
    Patent number: 5608036
    Abstract: A signal peptide sequence and nucleic acids encoding this sequence which are useful for improving the secretion efficiency of NGF polypeptides are provided. Also provided is a method for preparing Met-less NGF polypeptides.
    Type: Grant
    Filed: March 20, 1995
    Date of Patent: March 4, 1997
    Assignee: Amgen Inc.
    Inventors: Shi-Yuan Meng, Charles F. Morris, Larry B. Tsai
  • Modified OmpA signal sequence for enhanced secretion of polypeptides
    Patent number: 5470719
    Abstract: A signal peptide sequence and nucleic acids encoding this sequence which are useful for improving the secretion efficiency of NGF polypeptides are provided. Also provided is a method for preparing Met-less NGF polypeptides.
    Type: Grant
    Filed: March 18, 1994
    Date of Patent: November 28, 1995
    Inventors: Shi-Yuan Meng, Charles F. Morris, Larry B. Tsai
  • Production of biologically active insulin-like growth factor I from high expression host cell systems
    Patent number: 5158875
    Abstract: A novel method for refolding reduced IGF-I is provided in accordance with the present invention. The method is carried out by providing additional positive charge at the amino terminal end of the IGF-I molecule by the presence of a short leader sequence. The additional positive charge on the met-end of the IGF-I molecule has been found to enable recombinant IGF-I to refold simply by stirring solubilized inclusion body protein for between 2-16 hours, or overnight. The present invention also provides a novel fusion protein intermediate of IGF-I attached to the positively charged leader sequence.
    Type: Grant
    Filed: August 25, 1989
    Date of Patent: October 27, 1992
    Assignee: Amgen Inc.
    Inventors: James A. Miller, Philip K. Hsieh, Larry B. Tsai
  • Treating manganese-containing ores with a metal sulfide
    Patent number: 4752332
    Abstract: A process for treating a first metal, manganese-containing ore comprising contacting the manganese-containing ore with an aqueous, acidic composition and a material containing at least one metal sulfide at conditions effective to (1) chemically reduce at least a portion of the manganese, (2) solubilize at least a portion of the metal from the metal sulfide, and (3) at least partially liberate the first metal from the ore; and recovering the first metal from the ore. The use of Thiobacillus ferrooxidans and/or added ferric ion in the contacting step are also disclosed.
    Type: Grant
    Filed: April 30, 1986
    Date of Patent: June 21, 1988
    Assignee: Ensci, Inc.
    Inventors: Rebekah Wu, Larry B. Tsai, Barbara A. Krebs-Yuill, David A. Milligan, Nestor J. Troncoso, John S. McBride, Albert T. Knecht
  • Metal value recovery from metal sulfide containing ores
    Patent number: 4740243
    Abstract: A process for recovering at least one first metal from a metal sulfide-containing ore comprising contacting the ore with an aqueous, acidic composition and at least one reducible manganese-containing material at conditions effective (1) to chemically reduce the manganese, (2) solubilize at least a portion of the metal from the sulfide, and (3) at least partially liberate the first metal from the ore; and recovering the first metal from the ore. The use of added ferric ion and/or Thiobacillus ferrooxidans bacteria in the above-noted contacting is also disclosed.
    Type: Grant
    Filed: April 30, 1986
    Date of Patent: April 26, 1988
    Assignee: Ensci, Inc.
    Inventors: Barbara A. Krebs-Yuill, Larry B. Tsai, Rebekah Wu, David A. Milligan, Nestor J. Troncoso