Abstract: The present disclosure relates to a method and apparatus for a funnel assembly for use in a beverage brewing apparatus. The funnel assembly may include an insert that is retained in the funnel assembly to contain a powdered or other similar substance for mixing with liquid within the insert. The insert is configured to extend within a cavity of the funnel assembly and is configured to permit mixing of the substance with the liquid before dispensing the mixture into a portion of the funnel assembly. The insert may include a rim or liquid shield, with at least a portion of the rim or liquid shield engaging a portion of the funnel assembly to retain the insert within the funnel assembly. The insert may further include a flow guide adjacent a drainage aperture, the flow guide at least partially directing the flow of mixture from the insert into a portion of the funnel assembly.

Abstract: The present disclosure relates to a method and apparatus for a funnel assembly for use in a beverage brewing apparatus. The funnel assembly may include an insert that is retained in the funnel assembly to contain a powdered or other similar substance for mixing with liquid within the insert. The insert is configured to extend within a cavity of the funnel assembly and is configured to permit mixing of the substance with the liquid before dispensing the mixture into a portion of the funnel assembly. The insert may include a rim or liquid shield, with at least a portion of the rim or liquid shield engaging a portion of the funnel assembly to retain the insert within the funnel assembly. The insert may further include a flow guide adjacent a drainage aperture, the flow guide at least partially directing the flow of mixture from the insert into a portion of the funnel assembly.

Abstract: The present disclosure relates to a method and apparatus for a funnel assembly for use in a beverage brewing apparatus. The funnel assembly may include an insert that is retained in the funnel assembly to contain a powdered or other similar substance for mixing with liquid within the insert. The insert is configured to extend within a cavity of the funnel assembly and is configured to permit mixing of the substance with the liquid before dispensing the mixture into a portion of the funnel assembly. The insert may include a rim or liquid shield, with at least a portion of the rim or liquid shield engaging a portion of the funnel assembly to retain the insert within the funnel assembly. The insert may further include a flow guide adjacent a drainage aperture, the flow guide at least partially directing the flow of mixture from the insert into a portion of the funnel assembly.

Abstract: The present disclosure relates to a method and apparatus for a funnel assembly for use in a beverage brewing apparatus. The funnel assembly may include an insert that is retained in the funnel assembly to contain a powdered or other similar substance for mixing with liquid within the insert. The insert is configured to extend within a cavity of the funnel assembly and is configured to permit mixing of the substance with the liquid before dispensing the mixture into a portion of the funnel assembly. The insert may include a rim or liquid shield, with at least a portion of the rim or liquid shield engaging a portion of the funnel assembly to retain the insert within the funnel assembly. The insert may further include a flow guide adjacent a drainage aperture, the flow guide at least partially directing the flow of mixture from the insert into a portion of the funnel assembly.

Abstract: The present invention relates to the use of tumor suppressor genes in combination with a DNA damaging agent or factor for use in killing cells, and in particular cancerous cells. A tumor suppressor gene, p53, was delivered via a recombinant adenovirus-mediated gene transfer both in vitro and in vivo, in combination with a chemotherapeutic agent. Treated cells underwent apoptosis with specific DNA fragmentation. Direct injection of the p53-adenovirus construct into tumors subcutaneously, followed by intraperitoneal administration of a DNA damaging agent, cisplatin, induced massive apoptotic destruction of the tumors. The invention also provides for the clinical application of a regimen combining gene replacement using replication-deficient wild-type p53 adenovirus and DNA-damaging drugs for treatment of human cancer.

Abstract: The present invention relates to the use of tumor suppressor genes in combination with a DNA damaging agent or factor for use in killing cells, and in particular cancerous cells. A tumor suppressor gene, p53, was delivered via a recombinant adenovirus-mediated gene transfer both in vitro and in vivo, in combination with a chemotherapeutic agent. Treated cells underwent apoptosis with specific DNA fragmentation. Direct injection of the p53-adenovirus construct into tumors subcutaneously, followed by intraperitoneal administration of a DNA damaging agent, cisplatin, induced massive apoptotic destruction of the tumors. The invention also provides for the clinical application of a regimen combining gene replacement using replication-deficient wild-type p53 adenovirus and DNA-damaging drugs for treatment of human cancer.