Abstract: The present invention provides matched antibody pairs for the specific detection of one or more of the four dengue virus serotypes in a biological sample that may contain one or more of such dengue virus serotypes. Each matched antibody pair is capable of detecting not more than one serotype of dengue virus NS1 protein that may be present in the sample and will not cross react with other serotypes that may be present in the sample. Multiple matched pairs may be used to detect one or more dengue virus serotypes that may be present in a sample. Such matched pair antibodies, facilitate the development of confirmatory in vitro diagnostic tests such as sandwich immunoassays, that detect and distinguish the presence of one or more dengue virus serotypes in a biological sample, preferably a sample derived from human subject.

Abstract: The present invention provides matched antibody pairs for the specific detection of one or more of the four dengue virus serotypes in a biological sample that may contain one or more of such dengue virus serotypes. Each matched antibody pair is capable of detecting not more than one serotype of dengue virus NS1 protein that may be present in the sample and will not cross react with other serotypes that may be present in the sample. Multiple matched pairs may be used to detect one or more dengue virus serotypes that may be present in a sample. Such matched pair antibodies, facilitate the development of confirmatory in vitro diagnostic tests such as sandwich immunoassays, that detect and distinguish the presence of one or more dengue virus serotypes in a biological sample, preferably a sample derived from human subject.

Abstract: The present invention provides matched antibody pairs for the specific detection of one or more of the four dengue virus serotypes in a biological sample that may contain one or more of such dengue virus serotypes. Each matched antibody pair is capable of detecting not more than one serotype of dengue virus NS1 protein that may be present in the sample and will not cross react with other serotypes that may be present in the sample. Multiple matched pairs may be used to detect one or more dengue virus serotypes that may be present in a sample. Such matched pair antibodies, facilitate the development of confirmatory in vitro diagnostic tests such as sandwich immunoassays, that detect and distinguish the presence of one or more dengue virus serotypes in a biological sample, preferably a sample derived from human subject.

Abstract: In one example, a fluid system is provided that includes an air-operated fluid pump, a control air system operably connected with the fluid pump, and a pump controller operably connected with one or more components of the control air system. The pump controller is configured to automatically control the operation of the air-operated fluid pump by way of the control air system. The fluid pump parameters automatically controlled by the pump controller can include a discharge flow rate of the fluid pump, a discharge pressure of the fluid pump, and/or a pulsation value associated with the discharge of the fluid pump.

Abstract: In one example, a pump controller includes a power connection, one or more control signal connections, a feedback signal connection, one or more programmable elements, and one or more processors connected with the programmable elements and the feedback signal connection. When the programmable elements are programmed for automatic control of a fluid pump, the pump controller is operable to automatically control operation of the fluid pump through a closed loop system, and wherein the pump controller is further operable to control a pulse value associated with operation of the fluid pump.

Abstract: The present invention provides a multiplexed immunoassay which leverages stockpiled antibodies to detect whether a patient has been infected with an emerging disease which does not have specific antibodies raised against it (FIG. 1). The assay is preferably designed as a paper-based assay, which allows diagnosis at point of care (POC) and readout by eye or mobile phone. Paper-based rapid diagnostic tests (RDTs) are convenient, robust, and can be read out within minutes. The immunoassay of the invention combines the strategic use of nanoparticles of assorted colors with readily available stockpiled antibodies to one or more biomarkers of disease, particularly viral diseases.

Abstract: In one example, a valve includes a body defining a chamber, and a spool disposed in the chamber and configured to move within the chamber, such that a first operating position of the valve is defined when the spool is in a first position in the chamber, and a second operating position of the valve is defined when the spool is in a second position in the chamber. The valve also includes a magnetic element connected to the spool and responsive to a magnetic field such that the spool is biased in a first direction when the spool is in the first position and the spool is biased in a second direction when the spool is in the second position, and the second direction is different from the first direction.

Abstract: The present invention provides a lateral flow multiplex assay strip, and lateral flow assay systems and kits comprising the assay strips of the invention and methods of using the assay strip and systems and kits to detect the levels of two or more target analytes that may be present in a liquid sample, wherein the two more target analytes are detectable at a single test area of the assay.

Abstract: The present invention provides matched antibody pairs for the specific detection of one or more of the four dengue virus serotypes in a biological sample that may contain one or more of such dengue virus serotypes. Each matched antibody pair is capable of detecting not more than one serotype of dengue virus NS1 protein that may be present in the sample and will not cross react with other serotypes that may be present in the sample. Multiple matched pairs may be used to detect one or more dengue virus serotypes that may be present in a sample. Such matched pair antibodies, facilitate the development of confirmatory in vitro diagnostic tests such as sandwich immunoassays, that detect and distinguish the presence of one or more dengue virus serotypes in a biological sample, preferably a sample derived from human subject.

Abstract: Methods, devices, apparatus, assemblies, and kits for performing a vascular anastomosis are disclosed. A device for a vascular anastomosis includes tissue engaging portions that can move between at least two configurations. In some embodiments, the tissue engaging portions move without the aid of moving parts, while in other embodiments the tissue engaging portions extend from one or more movable wings. The tissue engaging portions may be separated by a first distance when in a pre-deployment configuration and by a second distance when in a deployed configuration. A method includes engaging a plurality of tissue engaging members of a coupling device against first end tissue. After selectively engaging the tissue engaging members and first end tissue, the first end tissue is stretched by at least moving the tissue engaging members. The stretched first end tissue is coupled to the second end tissue by mating the coupling device to a mating anastomosis device.

Abstract: In one example, a displacement pump has a suction side including a suction check valve, and a discharge side including a discharge check valve. The discharge side and suction side are each configured for selective fluid communication with each other, and the suction check valve defines a flow area that is relatively larger than a flow area defined by the discharge check valve.

Abstract: In one example, a fluid system is provided that includes an air-operated fluid pump, a control air system operably connected with the fluid pump, and a pump controller operably connected with one or more components of the control air system. The pump controller is configured to automatically control the operation of the air-operated fluid pump by way of the control air system. The fluid pump parameters automatically controlled by the pump controller can include a discharge flow rate of the fluid pump, a discharge pressure of the fluid pump, and/or a pulsation value associated with the discharge of the fluid pump.

Abstract: Exemplary embodiments provide diagnostic devices, systems and methods for determining the presence or absence of one or more markers or characteristics in one or more samples. An exemplary diagnostic device may display a first two-dimensional machine-readable output to indicate the presence or absence of a first characteristic in a sample. Similarly, the exemplary diagnostic device may display a second two-dimensional machine-readable output to indicate the presence or absence of a second characteristic in a sample. An image capture device may be used to automatically detect the two-dimensional machine-readable output appearing in the diagnostic device. A computational device may be used to automatically determine whether the presence or absence of the first characteristic and/or the second characteristic based on the two-dimensional machine-readable output displayed in the diagnostic device.

Abstract: Exemplary embodiments provide diagnostic devices, systems and methods for determining the presence or absence of one or more markers or characteristics in one or more samples. An exemplary diagnostic device may display a first two-dimensional machine-readable output to indicate the presence or absence of a first characteristic in a sample. Similarly, the exemplary diagnostic device may display a second two-dimensional machine-readable output to indicate the presence or absence of a second characteristic in a sample. An image capture device may be used to automatically detect the two-dimensional machine-readable output appearing in the diagnostic device. A computational device may be used to automatically determine whether the presence or absence of the first characteristic and/or the second characteristic based on the two-dimensional machine-readable output displayed in the diagnostic device.

Abstract: Methods, devices, apparatus, assemblies, and kits for performing a vascular anastomosis are disclosed. A device for a vascular anastomosis includes tissue engaging portions that can move between at least two configurations. In some embodiments, the tissue engaging portions move without the aid of moving parts, while in other embodiments the tissue engaging portions extend from one or more movable wings. The tissue engaging portions may be separated by a first distance when in a pre-deployment configuration and by a second distance when in a deployed configuration. A method includes engaging a plurality of tissue engaging members of a coupling device against first end tissue. After selectively engaging the tissue engaging members and first end tissue, the first end tissue is stretched by at least moving the tissue engaging members. The stretched first end tissue is coupled to the second end tissue by mating the coupling device to a mating anastomosis device.

Abstract: Disclosed are novel muteins of IL-1 compounds which can be used to regulate excess IL-1 produced in various diseases in humans and animals. The IL-1 muteins can be prepared by site-directed mutagenesis whereby a positively charged residue is replaced with a negatively charged or neutral residue at a designated position in the molecule. The resulting IL-1 muteins have reduced biological activity but retain receptor binding affinity.

Abstract: The subject invention concerns truncated human Il-1 cDNA sequences which encode biologically-active novel human IL-1 proteins. These truncated human IL-1 cDNA sequences can be obtained by genetic engineering procedures using a clone of human IL-1 cDNA, having the accession number NRRL B-15770, as a starting material. The truncated human IL-1 cDNA sequences of the subject invention are contained in specified plasmids whose constructions are described in detail. Biologically-active human IL-1 proteins are useful to induce the production of IL-2 by activated T-cells. They also act on B-cells and NK-cells.

Abstract: The subject invention concerns truncated human IL-1 cDNA sequences which encode biologically-active novel human IL-1 proteins. These truncated human IL-1 cDNA sequences can be obtained by genetic engineering procedures using a clone of human IL-1 cDNA, having the accession number NRRL B-15770, as a starting material. The truncated human IL-1 cDNA sequences of the subject invention are contained in specified plasmids whose constructions are described in detail. Biologically-active human IL-1 proteins are useful to induce the production of IL-2 by activated T-cells. They also act on B-cells and NK-cells.

Abstract: A recombinant plasmid which may be used for propagation of cloned cDNAs and also for the in vitro synthesis of RNA which is an exact copy of the natural sequence, wherein the transcript is devoid of vector-derived sequence. The novel vector was generated de novo by genetic engineering procedures using a synthetic double strand oligodeoxyribonucleotide fragment and a larger DNA fragment derived from plasmid pSP64. The vector, plasmid pHST-O, carried by Escherichia coli HB101, deposited with the ATCC on Dec.

Abstract: A process and means for increasing the production of protein translated from eukaryotic messenger ribonucleic acid (mRNA) comprising transferring a regulatory nucleotide sequence from a viral coat protein mRNA to the 5' terminus of a gene or complementary deoxyribonucleic acid (cDNA) encoding the protein to be produced to form a chimeric DNA sequence. The regulatory DNA sequence are generated de novo using genetic engineering procedures to produce synthetic double-stranded oligonucleotides representing the regulatory viral sequence. Nucleotide sequences which encode a regulatory sequence or structure conferring enhanced competitive activity and increased rate of translation upon the chimeric DNA sequences include the nucleotide sequence preceding the initiator AUG codon at the 5' terminus of coat protein messenger RNA from alfalfa mosaic virus, brome mosaic virus, black beetle virus, turnip yellow mosaic virus, and satellite tobacco necrosis virus.