Patents by Inventor Louis-Philippe Vezina

Louis-Philippe Vezina has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Publication number: 20240000107
    Abstract: The present disclosure relates to processes for recovering valuable products from Fabaceae family plant fractions, in particular from Medicago sativa ssp. The processes disclosed herein include processes for obtaining macrofibers, microfibers, a saponin precursor, chloroplast liquid and dry compositions and a Rubisco precursor. There is also disclosed herein processes for extracting from Fabaceae family plants valuable compounds such as proteins, enzymes, peptides, amino acids, fatty acids, fatty alcohols, terpenes, phenols and pigments. The processes may comprise at least one of separating plant fibers while attenuating shear forces, maintaining the temperature at or below 45° C., maintaining the pH above 4 and adding antioxidant and/or antimicrobial agents. Compositions comprising these recovered Fabaceae family plant products and uses thereof are also disclosed.
    Type: Application
    Filed: October 21, 2021
    Publication date: January 4, 2024
    Applicant: VIRENTIA INNOVATION INC.
    Inventors: Pierre LAVALLÉE, Réjean DESGAGNÉS, Laurence CAMBRON-FORTIN, Louis-Philippe VÉZINA, Pierre TALBOT
  • Publication number: 20230413853
    Abstract: The present disclosure relates to processes for recovering valuable products from Fabaceae family plant fractions, in particular from Medicago sativa ssp. The processes disclosed herein include processes for obtaining macrofibers, microfibers, a saponin precursor, chloroplast liquid and dry compositions and a Rubisco precursor. There is also disclosed herein processes for extracting from Fabaceae family plants valuable compounds such as proteins, enzymes, peptides, amino acids, fatty acids, fatty alcohols, terpenes, phenols and pigments. The processes may comprise at least one of separating plant fibers while attenuating shear forces, maintaining the temperature at or below 45° C., maintaining the pH above 4 and adding antioxidant and/or antimicrobial agents. Compositions comprising these recovered Fabaceae family plant products and uses thereof are also disclosed.
    Type: Application
    Filed: May 16, 2023
    Publication date: December 28, 2023
    Applicant: VIRENTIA INNOVATION INC.
    Inventors: Pierre LAVALLÉE, Réjean DESGAGNÉS, Laurence CAMBRON-FORTIN, Louis-Philippe VÉZINA, Pierre TALBOT
  • Patent number: 11833200
    Abstract: Methods of preparing plant-derived proteins or suprastructure proteins, are provided. The method may comprise obtaining a plant, or plant matter comprising apoplast-localized proteins, or suprastructure proteins, producing a protoplast/spheroplast fraction and apoplast fraction from the plant or plant matter, and recovering the apoplast fraction. The apoplast fraction comprises plant-derived proteins or suprastructure proteins. Alternatively, the proteins, or suprastructure proteins, may be obtained from plant or plant matter comprising plant-derived proteins or suprastructure proteins, by digesting the plant matter using a cell wall degrading enzyme composition to produced a digested fraction. The digested fraction is filtered to produced a filtered fraction, and the plant-derived proteins or suprastructure proteins, are recovered from the filtered fraction.
    Type: Grant
    Filed: September 21, 2010
    Date of Patent: December 5, 2023
    Assignee: MEDICAGO INC.
    Inventors: Louis-Philippe Vezina, Manon Couture, Dany Paquet, Michele Dargis, Marc-Andre D'Aoust
  • Patent number: 11826419
    Abstract: Methods of preparing plant-derived virus like particles (VLPs) are provided. The method may comprise obtaining a plant, or plant matter comprising apoplast-localized VLPs, producing a protoplast/spheroplast fraction and apoplast fraction from the plant or plant matter, and recovering the apoplast fraction. The apoplast fraction comprises plant-derived VLPs. Alternatively, VLPs may be obtained from plant or plant matter comprising plant-derived VLPs by digesting the plant matter using a cell wall degrading enzyme composition to produced a digested fraction. The digested fraction is filtered to produced a filtered fraction, and the plant-derived VLPs are recovered from the filtered fraction.
    Type: Grant
    Filed: September 21, 2010
    Date of Patent: November 28, 2023
    Assignee: MEDICAGO INC.
    Inventors: Louis-Philippe Vezina, Manon Couture, Dany Paquet, Michele Dargis, Marc-Andre D'Aoust
  • Patent number: 11672261
    Abstract: The present disclosure relates to processes for recovering valuable products from Fabaceae family plant fractions, in particular from Medicago sativa ssp. The processes disclosed herein include processes for obtaining macrofibers, microfibers, a saponin precursor, chloroplast liquid and dry compositions and a Rubisco precursor. There is also disclosed herein processes for extracting from Fabaceae family plants valuable compounds such as proteins, enzymes, peptides, amino acids, fatty acids, fatty alcohols, terpenes, phenols and pigments. The processes may comprise at least one of separating plant fibers while attenuating shear forces, maintaining the temperature at or below 45° C., maintaining the pH above 4 and adding antioxidant and/or antimicrobial agents. Compositions comprising these recovered Fabaceae family plant products and uses thereof are also disclosed.
    Type: Grant
    Filed: April 16, 2020
    Date of Patent: June 13, 2023
    Assignee: VIRENTIA INNOVATION INC.
    Inventors: Pierre Lavallée, Réjean Desgagnés, Laurence Cambron-Fortin, Louis-Philippe Vézina, Pierre Talbot
  • Publication number: 20230044454
    Abstract: A method for synthesizing influenza virus-like particles (VLPs) within a plant or a portion of a plant is provided. The method involves expression of influenza HA in plants and the purification by size exclusion chromatography. The invention is also directed towards a VLP comprising influenza HA protein and plant lipids. The invention is also directed to a nucleic acid encoding influenza HA as well as vectors. The VLPs may be used to formulate influenza vaccines, or may be used to enrich existing vaccines.
    Type: Application
    Filed: July 27, 2022
    Publication date: February 9, 2023
    Inventors: Marc-Andre D'AOUST, Manon COUTURE, Frederic ORS, Sonia TREPANIER, Pierre-Olivier LAVOIE, Michele DARGIS, Louis-Philippe VEZINA, Nathalie LANDRY
  • Patent number: 11434497
    Abstract: A method for synthesizing influenza virus-like particles (VLPs) within a plant or a portion of a plant is provided. The method involves expression of influenza HA in plants and the purification by size exclusion chromatography. The invention is also directed towards a VLP comprising influenza HA protein and plant lipids. The invention is also directed to a nucleic acid encoding influenza HA as well as vectors. The VLPs may be used to formulate influenza vaccines, or may be used to enrich existing vaccines.
    Type: Grant
    Filed: December 13, 2018
    Date of Patent: September 6, 2022
    Assignee: MEDICAGO INC.
    Inventors: Marc-Andre D'Aoust, Manon Couture, Frederic Ors, Sonia Trepanier, Pierre-Olivier Lavoie, Michele Dargis, Louis-Philippe Vezina, Nathalie Landry
  • Patent number: 11390878
    Abstract: A method of increasing the yield, stability, or both of an acid sensitive protein in a plant is provided. The method comprises introducing a first nucleic acid and a second nucleic acid into the plant, or portion of the plant. The first nucleic acid comprises a first regulatory region active in the plant and operatively linked to a nucleotide sequence encoding the acid sensitive protein. The second nucleic acid comprises a second regulatory region active in the plant and operatively linked to a nucleotide sequence encoding a channel protein, for example but not limited to a proton channel protein. The plant or portion of the plant is incubated under conditions that permit the expression of the nucleic acids, thereby increasing the yield of the acid sensitive protein when compared to the yield of the acid sensitive protein produced in the plant or portion of the plant produced under the same conditions, and in the absence of the proton channel protein.
    Type: Grant
    Filed: January 26, 2015
    Date of Patent: July 19, 2022
    Assignees: MEDICAGO INC., UNIVERSITE LAVAL
    Inventors: Marc-Andre D'Aoust, Manon Couture, Louis-Philippe Vezina, Dominique Michaud, Philippe Varennes-Jutras, Frank Sainsbury
  • Publication number: 20220211073
    Abstract: The present disclosure relates to processes for recovering valuable products from Fabaceae family plant fractions, in particular from Medicago sativa ssp. The processes disclosed herein include processes for obtaining macrofibers, microfibers, a saponin precursor, chloroplast liquid and dry compositions and a Rubisco precursor. There is also disclosed herein processes for extracting from Fabaceae family plants valuable compounds such as proteins, enzymes, peptides, amino acids, fatty acids, fatty alcohols, terpenes, phenols and pigments. The processes may comprise at least one of separating plant fibers while attenuating shear forces, maintaining the temperature at or below 45° C., maintaining the pH above 4 and adding antioxidant and/or antimicrobial agents. Compositions comprising these recovered Fabaceae family plant products and uses thereof are also disclosed.
    Type: Application
    Filed: April 16, 2020
    Publication date: July 7, 2022
    Applicant: VIRENTIA INNOVATION INC.
    Inventors: Pierre LAVALLÉE, Réjean DESGAGNÉS, Laurence CAMBRON-FORTIN, Louis-Philippe VÉZINA, Pierre TALBOT
  • Publication number: 20220145317
    Abstract: A method of producing a virus like particle (VLP) in a plant comprising modified H3 hemagglutinin is provided. The method comprises introducing a nucleic acid comprising a regulatory region active in the plant and operatively linked to a nucleotide sequence encoding a modified influenza hemagglutinin (HA) protein into the plant, or portion of the plant, the modified HA protein comprises a modified proteolytic loop. Followed by incubating the plant or portion of the plant under conditions that permit the expression of the nucleic acids, thereby producing the VLP. The modified proteolytic loop may comprise one or more protease cleavage sites exhibiting reduced or abolished cleavage by a protease. Also described is a virus like particle (VLP) produced by the method, and plants expressing the VLP. The virus like particle (VLP) may comprise plant-specific N-glycans, or modified N-glycans.
    Type: Application
    Filed: August 6, 2021
    Publication date: May 12, 2022
    Applicant: MEDICAGO INC.
    Inventors: Manon COUTURE, Marc-Andre D'AOUST, Louis-Philippe VEZINA
  • Patent number: 11155581
    Abstract: A method of producing a virus like particle (VLP) in a plant is provided. The method comprises introducing a first nucleic acid and a second nucleic acid into the plant, or portion of the plant. The first nucleic acid comprises a first regulatory region active in the plant and operatively linked to a nucleotide sequence encoding a structural virus protein. The second nucleic acid comprises a second regulatory region active in the plant and operatively linked to a nucleotide sequence encoding a channel protein, for example but not limited to a proton channel protein. The plant or portion of the plant is incubated under conditions that permit the expression of the nucleic acids, thereby producing the VLP.
    Type: Grant
    Filed: September 28, 2012
    Date of Patent: October 26, 2021
    Assignee: MEDICAGO INC.
    Inventors: Marc-Andre D'aoust, Manon Couture, Louis-Philippe Vezina
  • Patent number: 11085049
    Abstract: A method of producing a virus like particle (VLP) in a plant comprising modified hemagglutinin is provided. The method comprises introducing a nucleic acid comprising a regulatory region active in the plant and operatively linked to a nucleotide sequence encoding a modified influenza hemagglutinin (HA) protein into the plant, or portion of the plant, the modified HA protein comprises a modified proteolytic loop. Followed by incubating the plant or portion of the plant under conditions that permit the expression of the nucleic acids, thereby producing the VLP. The modified proteolytic loop may comprise one or more protease cleavage sites exhibiting reduced or abolished cleavage by a protease. The nucleotide sequence encoding the HA may be selected from the group consisting of B HA, C, H1, H2, H3, H4, H5, H6, H7, H8, H9, H10, H11, H12, H13, H14, H15, and H16. Also described is a virus like particle (VLP) produced by the method, and plants expressing the VLP.
    Type: Grant
    Filed: July 19, 2019
    Date of Patent: August 10, 2021
    Assignee: MEDICAGO INC.
    Inventors: Manon Couture, Marc-Andre D'Aoust, Louis-Philippe Vezina
  • Publication number: 20210023205
    Abstract: A method of producing a virus like particle (VLP) in a plant, and compositions comprising VLPs, are provided. The method involves introducing a nucleic acid comprising a regulatory region active in the plant and operatively linked to a chimeric nucleotide sequence encoding, in series, an ectodomain from a virus trimeric surface protein or fragment thereof, fused to an influenza transmembrane domain and cytoplasmic tail, into the plant, or portion of the plant, the ectodomain is from a non-influenza virus trimeric surface protein and heterologous with respect to the influenza transmembrane domain, and the cytoplasmic tail. The plant or portion of the plant are incubated under conditions that permit the expression of the nucleic acid, thereby producing the VLP. A VLP produced by this method are also provided.
    Type: Application
    Filed: October 9, 2020
    Publication date: January 28, 2021
    Applicant: MEDICAGO INC.
    Inventors: Marc-Andre D'Aoust, Manon Couture, Pierre-Olivier Lavoie, Louis-Philippe Vezina
  • Publication number: 20200353071
    Abstract: A method of producing a virus like particle (VLP) in a plant, and compositions comprising VLPs, are provided. The method involves introducing a nucleic acid comprising a regulatory region active in the plant and operatively linked to a chimeric nucleotide sequence encoding, in series, an ectodomain from a virus trimeric surface protein or fragment thereof, fused to an influenza transmembrane domain and cytoplasmic tail, into the plant, or portion of the plant, the ectodomain is from a non-influenza virus trimeric surface protein and heterologous with respect to the influenza transmembrane domain, and the cytoplasmic tail. The plant or portion of the plant are incubated under conditions that permit the expression of the nucleic acid, thereby producing the VLP. A VLP produced by this method are also provided.
    Type: Application
    Filed: March 26, 2020
    Publication date: November 12, 2020
    Applicant: MEDICAGO INC.
    Inventors: Marc-Andre D'Aoust, Manon Couture, Pierre-Olivier Lavoie, Louis-Philippe Vezina
  • Publication number: 20200255849
    Abstract: A method of producing a virus like particle (VLP) in a plant comprising modified hemagglutinin is provided. The method comprises introducing a nucleic acid comprising a regulatory region active in the plant and operatively linked to a nucleotide sequence encoding a modified influenza hemagglutinin (HA) protein into the plant, or portion of the plant, the modified HA protein comprises a modified proteolytic loop. Followed by incubating the plant or portion of the plant under conditions that permit the expression of the nucleic acids, thereby producing the VLP. The modified proteolytic loop may comprise one or more protease cleavage sites exhibiting reduced or abolished cleavage by a protease. The nucleotide sequence encoding the HA may be selected from the group consisting of B HA, C, H1, H2, H3, H4, H5, H6, H7, H8, H9, H10, H11, H12, H13, H14, H15, and H16. Also described is a virus like particle (VLP) produced by the method, and plants expressing the VLP.
    Type: Application
    Filed: July 19, 2019
    Publication date: August 13, 2020
    Applicant: MEDICAGO INC.
    Inventors: Manon COUTURE, Marc-Andre D'AOUST, Louis-Philippe VEZINA
  • Patent number: 10590173
    Abstract: A method of producing a picornavirus-like particle (PVLP) in a plant is provided. The method comprises introducing a first nucleic acid and a second nucleic acid into the plant, portion of the plant, or a plant cell. The first nucleic acid comprising a first regulatory region active in the plant operatively linked to a nucleotide sequence encoding a polyprotein. The second nucleic acid comprises a second regulatory region active in the plant and operatively linked to a nucleotide sequence encoding one or more protease. The plant, portion of the plant, or plant cell is incubated under conditions that permit the expression of the nucleic acids, thereby producing the PVLP. A PVLP comprising the polyprotein is also provided.
    Type: Grant
    Filed: December 20, 2018
    Date of Patent: March 17, 2020
    Assignee: Medicago Inc.
    Inventors: Marc-Andre D'Aoust, Pierre-Olivier Lavoie, Manon Couture, Lucie Poulin, Louis-Philippe Vezina
  • Patent number: 10477778
    Abstract: A plant tray for receiving and retaining one or more plant pots includes a plant-receiving tray for use in a high-throughput plant infiltration process used to produce recombinant proteins. The plant tray described has a base retaining tray with an upper surface defining cavities for receiving the plant pots, and a top plate displaceably mounted on the base retaining tray and having apertures, the apertures defining a channel with the corresponding cavities of the bottom tray when aligned. The top plate is displaceable between a locked operative position wherein the apertures and the corresponding cavities are partially misaligned, thereby locking the plant pots in between the top plate and the base retaining tray.
    Type: Grant
    Filed: May 28, 2013
    Date of Patent: November 19, 2019
    Assignee: MEDICAGO INC.
    Inventors: Louis-Philippe Vezina, Nicole Bechtold, Luc Laurin
  • Patent number: 10358652
    Abstract: A method of producing a virus like particle (VLP) in a plant comprising modified hemagglutinin is provided. The method comprises introducing a nucleic acid comprising a regulatory region active in the plant and operatively linked to a nucleotide sequence encoding a modified influenza hemagglutinin (HA) protein into the plant, or portion of the plant, the modified HA protein comprises a modified proteolytic loop. Followed by incubating the plant or portion of the plant under conditions that permit the expression of the nucleic acids, thereby producing the VLP. The modified proteolytic loop may comprise one or more protease cleavage sites exhibiting reduced or abolished cleavage by a protease. The nucleotide sequence encoding the HA may be selected from the group consisting of B HA, C, H1, H2, H3, H4, H5, H6, H7, H8, H9, H10, H11, H12, H13, H14, H15, and H16. Also described is a virus like particle (VLP) produced by the method, and plants expressing the VLP.
    Type: Grant
    Filed: March 28, 2014
    Date of Patent: July 23, 2019
    Assignee: MEDICAGO INC.
    Inventors: Manon Couture, Marc-Andre D'Aoust, Louis-Philippe Vezina
  • Publication number: 20190177739
    Abstract: A method for synthesizing influenza virus-like particles (VLPs) within a plant or a portion of a plant is provided. The method involves expression of influenza HA in plants and the purification by size exclusion chromatography. The invention is also directed towards a VLP comprising influenza HA protein and plant lipids. The invention is also directed to a nucleic acid encoding influenza HA as well as vectors. The VLPs may be used to formulate influenza vaccines, or may be used to enrich existing vaccines.
    Type: Application
    Filed: December 13, 2018
    Publication date: June 13, 2019
    Inventors: Marc-Andre D'AOUST, Manon COUTURE, Frederic ORS, Sonia TREPANIER, Pierre-Olivier LAVOIE, Michele DARGIS, Louis-Philippe VEZINA, Nathalie LANDRY
  • Publication number: 20190160167
    Abstract: The present invention relates to a type I or II transmembrane fusion protein comprising, successively: a) optionally, a signal peptide; b) a protein or a peptide of interest; c) a coiled-coil domain; and d) a domain for anchoring in the plasma membrane, consisting of a transmembrane segment and a cytosolic segment. It also relates to the virus-like particles obtained with this fusion protein.
    Type: Application
    Filed: July 28, 2017
    Publication date: May 30, 2019
    Inventors: Véronique GOMORD, Louis-Philippe VEZINA, Loïc FAYE, Virginie CATALA, Anne-Catherine FITCHETTE