Patents by Inventor Lyle J. Arnold, Jr.

Lyle J. Arnold, Jr. has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Patent number: 10196674
    Abstract: Improved methods for use in nucleic acid amplification, including multiplex amplification, where the amplification is carried out in two or more distinct phases are disclosed. The first phase amplification reaction preferably lacks one or more components required for exponential amplification. The lacking component is subsequently provided in a second, third or further phase(s) of amplification, resulting in a rapid exponential amplification reaction. The multiphase protocol results in faster and more sensitive detection and lower variability at low analyte concentrations. Compositions for carrying out the claimed methods are also disclosed.
    Type: Grant
    Filed: September 16, 2015
    Date of Patent: February 5, 2019
    Assignee: GEN-PROBE INCORPORATED
    Inventors: Norman C. Nelson, Lyle J. Arnold, Jr., Lizhong Dai, Steven Phelps, Jijumon Chelliserry
  • Patent number: 10179931
    Abstract: The present invention provides methods for immobilizing target nucleic acids on a solid support utilizing combinatorial capture probe pairs. These pairs contain first and second capture oligonucleotides that each comprise a target binding region, a capture region and a stem region positioned between the target binding and capture regions. The target binding regions comprise nucleic acid sequences that allow them to hybridize to adjacent regions on the target nucleic acid. The stem regions have nucleic acid sequences that are complementary to each other and the capture regions each comprise a sequence that when positioned adjacent to one another produce a combined nucleic acid sequence that is complementary to a portion of an oligonucleotide bound to a solid support.
    Type: Grant
    Filed: July 18, 2017
    Date of Patent: January 15, 2019
    Inventor: Lyle J. Arnold, Jr.
  • Patent number: 10119163
    Abstract: Compositions, reaction mixtures, and methods for performing an amplification reaction, including multiplex amplification reaction, wherein the method comprises using one or more amplification oligomer complexes comprising linked first and second amplification oligomer members. In one aspect, the amplification oligomer complex is hybridized to a target nucleic acid, the target nucleic acid with hybridized amplification oligomer complex is then captured, and other components are washed away. Target sequences of the target nucleic acids are pre-amplified to generate a first amplification product. The first amplification product is amplified in one or more secondary amplification reactions to generate second amplification products.
    Type: Grant
    Filed: March 2, 2016
    Date of Patent: November 6, 2018
    Assignee: GEN-PROBE INCORPORATED
    Inventors: Steven T. Brentano, Dmitry Lyakhov, Norman C. Nelson, James D. Carlson, Michael M. Becker, Lyle J. Arnold, Jr.
  • Publication number: 20180208981
    Abstract: Compositions, reaction mixtures, and methods for performing an amplification reaction, including multiplex amplification reaction, wherein the method comprises using one or more amplification oligomer complexes comprising linked first and second amplification oligomer members. In one aspect, the amplification oligomer complex is hybridized to a target nucleic acid, the target nucleic acid with hybridized amplification oligomer complex is then captured, and other components are washed away. Target sequences of the target nucleic acids are pre-amplified to generate a first amplification product. The first amplification product is amplified in one or more secondary amplification reactions to generate second amplification products.
    Type: Application
    Filed: February 22, 2018
    Publication date: July 26, 2018
    Inventors: Steven T. BRENTANO, Dmitry LYAKHOV, Norman C. NELSON, James D. CARLSON, Michael M. BECKER, Lyle J. ARNOLD, JR.
  • Patent number: 9677135
    Abstract: Compositions that are used in nucleic acid amplification in vitro are disclosed, which include a target specific universal (TSU) promoter primer or promoter provider oligonucleotide that includes a target specific (TS) sequence that hybridizes specifically to a target sequence that is amplified and a universal (U) sequence that is introduced into the sequence that is amplified, by using a primer for the universal sequence. Methods of nucleic acid amplification in vitro are disclosed that use one or more TSU oligonucleotides to attached a U sequence to a target nucleic acid in a target capture step and then use a primer for a U sequence in subsequent amplification steps performed in substantially isothermal conditions to make amplification products that contain a U sequence that indicates the presence of the target nucleic acid in a sample.
    Type: Grant
    Filed: December 17, 2013
    Date of Patent: June 13, 2017
    Assignee: GEN-PROBE INCORPORATED
    Inventors: Steven T. Brentano, Dmitry Lyakhov, James D. Carlson, Norman C. Nelson, Lyle J. Arnold, Jr., Michael M. Becker
  • Publication number: 20160348162
    Abstract: Compositions, reaction mixtures, and methods for performing an amplification reaction, including multiplex amplification reaction, wherein the method comprises using one or more amplification oligomer complexes comprising linked first and second amplification oligomer members. In one aspect, the amplification oligomer complex is hybridized to a target nucleic acid, the target nucleic acid with hybridized amplification oligomer complex is then captured, and other components are washed away. Target sequences of the target nucleic acids are pre-amplified to generate a first amplification product. The first amplification product is amplified in one or more secondary amplification reactions to generate second amplification products.
    Type: Application
    Filed: March 2, 2016
    Publication date: December 1, 2016
    Inventors: Steven T. BRENTANO, Dmitry LYAKHOV, Norman C. NELSON, James D. CARLSON, Michael M. BECKER, Lyle J. ARNOLD, Jr.
  • Patent number: 9399796
    Abstract: Compositions, reaction mixtures, and methods for performing an amplification reaction, including multiplex amplification reaction, wherein the method comprises using one or more amplification oligomer complexes comprising linked first and second amplification oligomer members. In one aspect, the amplification oligomer complex is hybridized to a target nucleic acid, the target nucleic acid with hybridized amplification oligomer complex is then captured, and other components are washed away. Target sequences of the target nucleic acids are pre-amplified to generate a first amplification product. The first amplification product is amplified in one or more secondary amplification reactions to generate second amplification products.
    Type: Grant
    Filed: July 31, 2013
    Date of Patent: July 26, 2016
    Assignee: GEN-PROBE INCORPORATED
    Inventors: Steven T. Brentano, Dmitry Lyakhov, Norman C. Nelson, James D. Carlson, Michael M. Becker, Lyle J. Arnold, Jr.
  • Patent number: 9243286
    Abstract: This invention relates to oligonucleotides comprising a molecular switch which may exist in an “open” or “closed” position. The molecular switch portion of the probe is particularly sensitive to the identity of sequences complementary to the molecular switch. Oligonucleotides containing a molecular switch are applicable to all kinds of hybridization processes. Due to the sensitivity of the switch domain of the oligonucleotide, probes containing a molecular switch are particularly useful in the identification of single point mismatches. More specifically, a portion, but not all, of the oligonucleotide becomes unbound from a mismatched target. The invention further relates to methods of using said oligonucleotides for research reagents, and clinical diagnostics. An exemplary oligonucleotide comprises a first hybridizable domain, a second bridging block domain, and a third binding domain.
    Type: Grant
    Filed: January 13, 2014
    Date of Patent: January 26, 2016
    Assignee: GEN-PROBE INCORPORATED
    Inventors: Lyle J. Arnold, Jr., Bob D. Brown
  • Publication number: 20160002709
    Abstract: Improved methods for use in nucleic acid amplification, including multiplex amplification, where the amplification is carried out in two or more distinct phases are disclosed. The first phase amplification reaction preferably lacks one or more components required for exponential amplification. The lacking component is subsequently provided in a second, third or further phase(s) of amplification, resulting in a rapid exponential amplification reaction. The multiphase protocol results in faster and more sensitive detection and lower variability at low analyte concentrations. Compositions for carrying out the claimed methods are also disclosed.
    Type: Application
    Filed: September 16, 2015
    Publication date: January 7, 2016
    Inventors: Norman C. NELSON, Lyle J. ARNOLD, JR., Lizhong DAI, Steven PHELPS, Jijumon CHELLISERRY
  • Patent number: 9169512
    Abstract: Compositions, reaction mixtures, and methods for performing an amplification reaction, including multiplex amplification reaction, wherein the method comprises using one or more amplification oligomer complexes comprising linked first and second amplification oligomer members. In one aspect, the amplification oligomer complex is hybridized to a target nucleic acid, the target nucleic acid with hybridized amplification oligomer complex is then captured, and other components are washed away. Target sequences of the target nucleic acids are pre-amplified to generate a first amplification product. The first amplification product is amplified in one or more secondary amplification reactions to generate second amplification products.
    Type: Grant
    Filed: July 1, 2010
    Date of Patent: October 27, 2015
    Assignee: Gen-Probe Incorporated
    Inventors: Steven T. Brentano, Dmitry Lyakhov, James D. Carlson, Michael M. Becker, Norman C. Nelson, Lyle J. Arnold, Jr.
  • Patent number: 9139870
    Abstract: Improved methods for use in nucleic acid amplification, including multiplex amplification, where the amplification is carried out in two or more distinct phases are disclosed. The first phase amplification reaction preferably lacks one or more components required for exponential amplification. The lacking component is subsequently provided in a second, third or further phase(s) of amplification, resulting in a rapid exponential amplification reaction. The multiphase protocol results in faster and more sensitive detection and lower variability at low analyte concentrations. Compositions for carrying out the claimed methods are also disclosed.
    Type: Grant
    Filed: August 30, 2013
    Date of Patent: September 22, 2015
    Assignee: Gen-Probe Incorporated
    Inventors: Norman C. Nelson, Lyle J. Arnold, Jr., Lizhong Dai, Steven Phelps, Jijumon Chelliserry
  • Publication number: 20140193815
    Abstract: This invention relates to oligonucleotides comprising a molecular switch which may exist in an “open” or “closed” position. The molecular switch portion of the probe is particularly sensitive to the identity of sequences complementary to the molecular switch. Oligonucleotides containing a molecular switch are applicable to all kinds of hybridization processes. Due to the sensitivity of the switch domain of the oligonucleotide, probes containing a molecular switch are particularly useful in the identification of single point mismatches. More specifically, a portion, but not all, of the oligonucleotide becomes unbound from a mismatched target. The invention further relates to methods of using said oligonucleotides for research reagents, and clinical diagnostics. An exemplary oligonucleotide comprises a first hybridizable domain, a second bridging block domain, and a third binding domain.
    Type: Application
    Filed: January 13, 2014
    Publication date: July 10, 2014
    Applicant: Gen-Probe Incorporated
    Inventors: Lyle J. Arnold, JR., Bob D. Brown
  • Publication number: 20140127700
    Abstract: Compositions that are used in nucleic acid amplification in vitro are disclosed, which include a target specific universal (TSU) promoter primer or promoter provider oligonucleotide that includes a target specific (TS) sequence that hybridizes specifically to a target sequence that is amplified and a universal (U) sequence that is introduced into the sequence that is amplified, by using a primer for the universal sequence. Methods of nucleic acid amplification in vitro are disclosed that use one or more TSU oligonucleotides to attached a U sequence to a target nucleic acid in a target capture step and then use a primer for a U sequence in subsequent amplification steps performed in substantially isothermal conditions to make amplification products that contain a U sequence that indicates the presence of the target nucleic acid in a sample.
    Type: Application
    Filed: December 17, 2013
    Publication date: May 8, 2014
    Applicant: GEN-PROBE INCORPORATED
    Inventors: Steven T. BRENTANO, Dmitry LYAKHOV, James D. CARLSON, Norman C. NELSON, Lyle J. ARNOLD, JR., Michael M. BECKER
  • Patent number: 8679789
    Abstract: This invention relates to oligonucleotides comprising a molecular switch which may exist in an “open” or “closed” position. The molecular switch portion of the probe is particularly sensitive to the identity of sequences complementary to the molecular switch. Oligonucleotides containing a molecular switch are applicable to all kinds of hybridization processes. Due to the sensitivity of the switch domain of the oligonucleotide, probes containing a molecular switch are particularly useful in the identification of single point mismatches. More specifically, a portion, but not all, of the oligonucleotide becomes unbound from a mismatched target. The invention further relates to methods of using said oligonucleotides for research reagents, and clinical diagnostics. An exemplary oligonucleotide comprises a first hybridizable domain, a second bridging block domain, and a third binding domain.
    Type: Grant
    Filed: April 30, 2004
    Date of Patent: March 25, 2014
    Assignee: Gen-Probe Incorporated
    Inventors: Lyle J. Arnold, Jr., Bob D. Brown
  • Publication number: 20140066326
    Abstract: Improved methods for use in nucleic acid amplification, including multiplex amplification, where the amplification is carried out in two or more distinct phases are disclosed. The first phase amplification reaction preferably lacks one or more components required for exponential amplification. The lacking component is subsequently provided in a second, third or further phase(s) of amplification, resulting in a rapid exponential amplification reaction. The multiphase protocol results in faster and more sensitive detection and lower variability at low analyte concentrations. Compositions for carrying out the claimed methods are also disclosed.
    Type: Application
    Filed: August 30, 2013
    Publication date: March 6, 2014
    Applicant: GEN-PROBE INCORPORATED
    Inventors: Norman C. NELSON, Lyle J. ARNOLD, JR., Lizhong DAI, Steven PHELPS, Jijumon CHELLISERRY
  • Patent number: 8642268
    Abstract: Compositions that are used in nucleic acid amplification in vitro are disclosed, which include a target specific universal (TSU) promoter primer or promoter provider oligonucleotide that includes a target specific (TS) sequence that hybridizes specifically to a target sequence that is amplified and a universal (U) sequence that is introduced into the sequence that is amplified, by using a primer for the universal sequence. Methods of nucleic acid amplification in vitro are disclosed that use one or more TSU oligonucleotides to attached a U sequence to a target nucleic acid in a target capture step and then use a primer for a U sequence in subsequent amplification steps performed in substantially isothermal conditions to make amplification products that contain a U sequence that indicates the presence of the target nucleic acid in a sample.
    Type: Grant
    Filed: April 30, 2012
    Date of Patent: February 4, 2014
    Assignee: Gen-Probe Incorporated
    Inventors: Steven T. Brentano, Dmitry Lyakhov, James D. Carlson, Norman C. Nelson, Lyle J. Arnold, Jr., Michael M. Becker
  • Publication number: 20130309673
    Abstract: Compositions, reaction mixtures, and methods for performing an amplification reaction, including multiplex amplification reaction, wherein the method comprises using one or more amplification oligomer complexes comprising linked first and second amplification oligomer members. In one aspect, the amplification oligomer complex is hybridized to a target nucleic acid, the target nucleic acid with hybridized amplification oligomer complex is then captured, and other components are washed away. Target sequences of the target nucleic acids are pre-amplified to generate a first amplification product. The first amplification product is amplified in one or more secondary amplification reactions to generate second amplification products.
    Type: Application
    Filed: July 31, 2013
    Publication date: November 21, 2013
    Applicant: Gen-Probe Incorporated
    Inventors: Steven T. BRENTANO, Dmitry LYAKHOV, Norman C. NELSON, James D. CARLSON, Michael M. BECKER, Lyle J. ARNOLD, JR.
  • Patent number: 8512955
    Abstract: Compositions, reaction mixtures, and methods for performing an amplification reaction, including multiplex amplification reaction, wherein the method comprises using one or more amplification oligomer complexes comprising linked first and second amplification oligomer members. In one aspect, the amplification oligomer complex is hybridized to a target nucleic acid, the target nucleic acid with hybridized amplification oligomer complex is then captured, and other components are washed away. Target sequences of the target nucleic acids are pre-amplified to generate a first amplification product. The first amplification product is amplified in one or more secondary amplification reactions to generate second amplification products.
    Type: Grant
    Filed: July 1, 2010
    Date of Patent: August 20, 2013
    Assignee: Gen-Probe Incorporated
    Inventors: Steven T. Brentano, Dmitry Lyakhov, Norman C. Nelson, James D. Carlson, Michael M. Becker, Lyle J. Arnold, Jr.
  • Publication number: 20120264122
    Abstract: Compositions that are used in nucleic acid amplification in vitro are disclosed, which include a target specific universal (TSU) promoter primer or promoter provider oligonucleotide that includes a target specific (TS) sequence that hybridizes specifically to a target sequence that is amplified and a universal (U) sequence that is introduced into the sequence that is amplified, by using a primer for the universal sequence. Methods of nucleic acid amplification in vitro are disclosed that use one or more TSU oligonucleotides to attached a U sequence to a target nucleic acid in a target capture step and then use a primer for a U sequence in subsequent amplification steps performed in substantially isothermal conditions to make amplification products that contain a U sequence that indicates the presence of the target nucleic acid in a sample.
    Type: Application
    Filed: April 30, 2012
    Publication date: October 18, 2012
    Applicant: GEN-PROBE INCORPORATED
    Inventors: Steven T. BRENTANO, Dmitry LYAKHOV, James D. CARLSON, Norman C. NELSON, Lyle J. ARNOLD, JR.
  • Publication number: 20120178636
    Abstract: Compositions, reaction mixtures, and methods for performing an amplification reaction, including multiplex amplification reaction, wherein the method comprises using one or more amplification oligomer complexes comprising linked first and second amplification oligomer members. In one aspect, the amplification oligomer complex is hybridized to a target nucleic acid, the target nucleic acid with hybridized amplification oligomer complex is then captured, and other components are washed away. Target sequences of the target nucleic acids are pre-amplified to generate a first amplification product. The first amplification product is amplified in one or more secondary amplification reactions to generate second amplification products.
    Type: Application
    Filed: July 1, 2010
    Publication date: July 12, 2012
    Applicant: GEN-PROBE INCORPORATED
    Inventors: Steven T. Brentano, Dmitry Lyakhov, James D. Carlson, Michael M. Becker, Norman C. Nelson, Lyle J. Arnold, JR.