Patents by Inventor Makiko Fukushima

Makiko Fukushima has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Cultivation of transformed microorganisms
    Patent number: 5279951
    Abstract: A process for cultivating a microorganism transformed with a recombinant plasmid at least containing (a) a DNA fragment containing a promoter and a regulator gene tnaC located downstream of the promoter in a tryptophanase operon (tna), and (b) a DNA fragment containing a desired structural gene which can be expressed by the promoter, which comprises cultivating the transformed microorganism in a culture medium while adding glucose as a carbon source continuously or intermittently so that the concentration of glucose is maintained within the range of 0.01 to 0.3%, and thereby allowing the desired structural gene to be expressed in the microorganism.
    Type: Grant
    Filed: May 7, 1990
    Date of Patent: January 18, 1994
    Assignee: Research Association for Utilization of Light Oil
    Inventors: Masato Terasawa, Hisashi Yamagata, Hideaki Yukawa, Yasurou Kurusu, Makiko Fukushima
  • DNA fragment containing gene which encodes the function of stabilizing plasmid in host microorganism
    Patent number: 5185262
    Abstract: A DNA fragment derived from plasmid pBY503 obtained from Brevibacterium stationis IF012144, said DNA fragment containing a gene which encodes for the function of maintaining a plasmid, capable of replicating and proliferating at least in a Coryneform bacteria of the genus Brevibacterium, stably in said bacteria; and a vector DNA capable of replicating and proliferating in a Coryneform bacteria into which the above DNA fragment is introduced.
    Type: Grant
    Filed: February 1, 1990
    Date of Patent: February 9, 1993
    Assignee: Mitsubishi Petrochemical Co., Ltd.
    Inventors: Keiko Kohama, Miki Kobayashi, Yasurou Kurusu, Hideaki Yukawa, Makiko Fukushima
  • Method for producing L-threonine, and plasmid and microorganism employed in the same
    Patent number: 5153123
    Abstract: A method for producing L-threonine, which comprises subjecting at least L- or DL-aspartic acid or a salt thereof to enzymatic reaction according to the reaction system not accompanied with growth of microorganism cells in an aqueous solution in the presence of a microorganism and collecting L-threonine formed, wherein the microorganism is a biotin-requiring microorganism for the growth belonging to coryneform bacterium; a plasmid comprising a DNA fragment containing at least a gene encoding biosynthesis of threonine which can be expressed within a biotin-requiring microorganism cell for the growth belonging to coryneform bacterium and a DNA fragment containing a gene encoding autonomous replication within coryneform bacterium cell; and a biotin-requiring microorganism for the growth belonging to coryneform bacterium which has been transformed with the plasmid described above, both of which are employed in the present method.
    Type: Grant
    Filed: October 14, 1988
    Date of Patent: October 6, 1992
    Assignee: Mitsubishi Petrochemical Co., Ltd.
    Inventors: Masato Terasawa, Terukazu Nara, Makiko Fukushima, Yukie Satoo, Mitsunobu Shimazu, Hideaki Yukawa, Yasurou Kurusu, Keiko Kohama