Abstract: Provided is a fluorescence immunoassay sensor chip and a fluorescence immunoassay method, which are capable of measuring, at the same time, a marker requiring high sensitivity due to its low content in a sample solution and a marker not requiring high sensitivity due to its high content in a sample solution. The fluorescence immunoassay sensor chip for use in fluorescence immunoassay for detecting and measuring markers contained in a sample solution includes: a dielectric member; a metal thin film formed on part of a main surface of the dielectric member; a first sensor part formed in a predetermined position on the metal thin film; and a second sensor part directly formed in a predetermined position on the dielectric member, wherein a ligand immobilized in the first sensor part and a ligand immobilized in the second sensor part capture different types of markers.

Abstract: A detection device is provided with a holder, light irradiation unit, signal detection unit, and processing unit. The holder holds a detection chip. The light irradiation unit irradiates light onto the detection chip held by the holder. When the light irradiation unit irradiates the detection chip with light, the signal detection unit detects, at least twice, a signal generated at a reaction site. The processing unit calculates a signal value variation rate on the basis of a first signal value detected by the signal detection unit and a second signal value detected after the first signal value.

Abstract: At least an embodiment addresses the problem of providing a SPR (surface plasmon resonance) or SPFS (surface plasmon-field enhanced fluorescence spectroscopy) immunoassay, which enables the measurement of whole blood, undergoes little fluctuations in measurement values, and can measure whole blood, serum and plasma in a single apparatus. At least an embodiment solves the above-mentioned problem by a SPR or SPFS immunoassay, which is an immunoassay utilizing SPR or SPFS, including an absorbance measurement step of measuring an absorbance of a sample, a mode setting step of setting a mode that corresponds to the result of the absorbance measured in the absorbance measurement step, and one or multiple step(s) for which treatment condition(s) has/have been set in accordance with the mode set in the mode setting step.

Abstract: Provided is an assay method utilizing surface plasmon-field enhanced fluorescence spectroscopy, which suppresses fluctuations in an absolute value of a background signal that is referred to as a resonance angle or an optical blank by improving the effect when a whole blood sample is used, specifically, the effect by the blood cell components remaining in a measurement liquid during a SPFS measurement, with regards to a photometric condition setting step, which is a characteristic step in assay methods utilizing SPFS. The above-mentioned problem is solved by an assay method utilizing surface plasmon-field enhanced fluorescence spectroscopy, including a primary reaction step of a sample, a first washing step, a labeling reaction step, and a measurement step, wherein the method includes a step for evaluating a blood cell component quantity between the primary reaction step and the labeling reaction step.

Abstract: With a blood-derived specimen on top of a metal film on a measurement chip, the intensity of reflected excitation light, the resonance angle of excitation light, the intensity of plasmon-scattered light, or the enhancement angle of excitation light is measured, and the acquired measurement is used to obtain a whole-blood hematocrit value. Using the obtained whole-blood hematocrit value, a first signal value that indicates how much of an analyte the specimen contains is converted to a second signal value that indicates how much of said analyte the liquid part of the specimen contains.

Abstract: With a blood-derived specimen on top of a metal film on a measurement chip, the intensity of reflected excitation light, the resonance angle of excitation light, the intensity of plasmon-scattered light, or the enhancement angle of excitation light is measured, and the acquired measurement is used to obtain a whole-blood hematocrit value. Using the obtained whole-blood hematocrit value, a first signal value that indicates how much of an analyte the specimen contains is converted to a second signal value that indicates how much of said analyte the liquid part of the specimen contains.

Abstract: Provided is a fluorescence immunoassay sensor chip and a fluorescence immunoassay method, which are capable of measuring, at the same time, a marker requiring high sensitivity due to its low content in a sample solution and a marker not requiring high sensitivity due to its high content in a sample solution. The fluorescence immunoassay sensor chip for use in fluorescence immunoassay for detecting and measuring markers contained in a sample solution includes: a dielectric member; a metal thin film formed on part of a main surface of the dielectric member; a first sensor part formed in a predetermined position on the metal thin film; and a second sensor part directly formed in a predetermined position on the dielectric member, wherein a ligand immobilized in the first sensor part and a ligand immobilized in the second sensor part capture different types of markers.

Abstract: Provided is an assay method utilizing surface plasmon-field enhanced fluorescence spectroscopy, which suppresses fluctuations in an absolute value of a background signal that is referred to as a resonance angle or an optical blank by improving the effect when a whole blood sample is used, specifically, the effect by the blood cell components remaining in a measurement liquid during a SPFS measurement, with regards to a photometric condition setting step, which is a characteristic step in assay methods utilizing SPFS. The above-mentioned problem is solved by an assay method utilizing surface plasmon-field enhanced fluorescence spectroscopy, including a primary reaction step of a sample, a first washing step, a labeling reaction step, and a measurement step, wherein the method includes a step for evaluating a blood cell component quantity between the primary reaction step and the labeling reaction step.

Abstract: At least an embodiment addresses the problem of providing a SPR (surface plasmon resonance) or SPFS (surface plasmon-field enhanced fluorescence spectroscopy) immunoassay, which enables the measurement of whole blood, undergoes little fluctuations in measurement values, and can measure whole blood, serum and plasma in a single apparatus. At least an embodiment solves the above-mentioned problem by a SPR or SPFS immunoassay, which is an immunoassay utilizing SPR or SPFS, including an absorbance measurement step of measuring an absorbance of a sample, a mode setting step of setting a mode that corresponds to the result of the absorbance measured in the absorbance measurement step, and one or multiple step(s) for which treatment condition(s) has/have been set in accordance with the mode set in the mode setting step.