Patents by Inventor Mamiko Tsuchiya
Mamiko Tsuchiya has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Patent number: 8323917Abstract: The present invention enables screening for compounds that inhibit the transport of GPI-anchored proteins to fungal cell walls, using a simple assay for transacylation to GlcN-PI using membrane fraction expressing GWT1 protein. New antifungal agents can be created that inhibit the synthesis of fungal cell walls and also inhibit adhesion to host cells by inhibiting the transport of GPI-anchored proteins to fungal cell walls.Type: GrantFiled: November 21, 2003Date of Patent: December 4, 2012Assignees: National Institute of Advanced Industrial Science and Technology, Eisai R&D Management Co., Ltd.Inventors: Kappei Tsukahara, Mamiko Tsuchiya, Yoshifumi Jigami, Kenichi Nakayama, Mariko Umemura, Michiyo Okamoto
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Patent number: 7999090Abstract: The present invention provides isolated DNA encoding a GWT1 protein having activity to confer resistance of a fungus against a compound of formula Ia, and wherein a defect of a function of the GWT1 protein leads to a decrease in the amount of a glycosylphosphatidylinositol (GPI)-anchored protein in the cell wall of a fungus.Type: GrantFiled: April 15, 2008Date of Patent: August 16, 2011Assignee: Eisai Co., LtdInventors: Kappei Tsukahara, Katsura Hata, Koji Sagane, Kazutaka Nakamoto, Mamiko Tsuchiya, Naoaki Watanabe, Fuminori Ohba, Itaru Tsukada, Norihiro Ueda, Keigo Tanaka, Junko Kai
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Patent number: 7928209Abstract: A reporter system reflecting the transport process that transports GPI-anchored proteins to the cell wall was constructed and compounds inhibiting this process were discovered. Further, fungal genes conferring resistance to the above compounds were identified and methods of screening for compounds that inhibit the activity of the proteins encoded by these genes were developed. These genes encode proteins participating in fungal cell wall synthesis. Therefore, through the novel compounds, the present invention showed that antifungal agents having a novel mechanism, i.e. inhibiting the process that transports GPI-anchored proteins to the cell wall, could be achieved.Type: GrantFiled: December 17, 2007Date of Patent: April 19, 2011Assignee: Eisai R & D Management Co. Ltd.Inventors: Kappei Tsukahara, Katsura Hata, Koji Sagane, Kazutaka Nakamoto, Mamiko Tsuchiya, Naoaki Watanabe, Fuminori Ohba, Itaru Tsukada, Norihiro Ueda, Keigo Tanaka, Junko Kai
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Patent number: 7910712Abstract: The present invention provides isolated DNA encoding a GWT1 protein having activity to confer resistance of a fungus against a compound of formula Ia, and wherein a defect of a function of the GWT1 protein leads to a decrease in the amount of a glycosylphosphatidylinositol (GPI)-anchored protein in the cell wall of a fungus.Type: GrantFiled: April 15, 2008Date of Patent: March 22, 2011Assignee: Eisai R&D Management Co., LtdInventors: Kappei Tsukahara, Katsura Hata, Koji Sagane, Kazutaka Nakamoto, Mamiko Tsuchiya, Naoaki Watanabe, Fuminori Ohba, Itaru Tsukada, Norihiro Ueda, Keigo Tanaka, Junko Kai
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Patent number: 7897387Abstract: A reporter system reflecting the transport process that transports GPI-anchored proteins to the cell wall was constructed and compounds inhibiting this process were discovered. Further, genes conferring resistance to the above compounds were identified and methods of screening for compounds that inhibit the activity of the proteins encoded by these genes were developed. Therefore, through the novel compounds, the present invention showed that antifungal agents having a novel mechanism, i.e. inhibiting the process that transports GPI-anchored proteins to the cell wall, could be achieved.Type: GrantFiled: April 1, 2008Date of Patent: March 1, 2011Assignee: Eisai R&D Management Co., Ltd.Inventors: Kappei Tsukahara, Katsura Hata, Koji Sagane, Kazutaka Nakamoto, Mamiko Tsuchiya, Naoaki Watanabe, Fuminori Ohba, Itaru Tsukada, Norihiro Ueda, Keigo Tanaka, Junko Kai
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Publication number: 20090325228Abstract: A reporter system reflecting the transport process that transports GPI-anchored proteins to the cell wall was constructed and compounds inhibiting this process were discovered. Further, genes conferring resistance to the above compounds were identified and methods of screening for compounds that inhibit the activity of the proteins encoded by these genes were developed. Therefore, through the novel compounds, the present invention showed that antifungal agents having a novel mechanism, i.e. inhibiting the process that transports GPI-anchored proteins to the cell wall, could be achieved.Type: ApplicationFiled: April 15, 2008Publication date: December 31, 2009Applicant: EISAI R&D MANAGEMENT CO., LTD.Inventors: Kappei Tsukahara, Katsura Hata, Koji Sagane, Kazutaka Nakamoto, Mamiko Tsuchiya, Naoaki Watanabe, Fuminori Oba, Itaru Tsukada, Norihiro Ueda, Keigo Tanaka, Junko Kai
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Patent number: 7541332Abstract: A reporter system reflecting the transport process that transports GPI-anchored proteins to the cell wall was constructed and compounds inhibiting this process were discovered. Further, genes conferring resistance to the above compounds were identified and methods of screening for compounds that inhibit the activity of the proteins encoded by these genes were developed. Therefore, through the novel compounds, the present invention showed that antifungal agents having a novel mechanism, i.e. inhibiting the process that transports GPI-anchored proteins to the cell wall, could be achieved.Type: GrantFiled: April 21, 2006Date of Patent: June 2, 2009Assignee: Eisai R&D Management Co., Ltd.Inventors: Kappei Tsukahara, Katsura Hata, Koji Sagane, Kazutaka Nakamoto, Mamiko Tsuchiya, Naoaki Watanabe, Fuminori Ohba, Itaru Tsukada, Norihiro Ueda, Keigo Tanaka, Junko Kai
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Publication number: 20090117586Abstract: A reporter system reflecting the transport process that transports GPI-anchored proteins to the cell wall was constructed and compounds inhibiting this process were discovered. Further, genes conferring resistance to the above compounds were identified and methods of screening for compounds that inhibit the activity of the proteins encoded by these genes were developed. Therefore, through the novel compounds, the present invention showed that antifungal agents having a novel mechanism, i.e. inhibiting the process that transports GPI-anchored proteins to the cell wall, could be achieved.Type: ApplicationFiled: April 1, 2008Publication date: May 7, 2009Applicant: EISAI R&D MANAGEMENT CO., LTD.Inventors: KAPPEI TSUKAHARA, KATSURA HATA, KOJI SAGANE, KAZUTAKA NAKAMOTO, MAMIKO TSUCHIYA, NAOAKI WATANABE, FUMINORI OHBA, ITARU TSUKADA, NORIHIRO UEDA, KEIGO TANAKA, JUNKO KAI
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Publication number: 20090098636Abstract: A reporter system reflecting the transport process that transports GPI-anchored proteins to the cell wall was constructed and compounds inhibiting this process were discovered. Further, genes conferring resistance to the above compounds were identified and methods of screening for compounds that inhibit the activity of the proteins encoded by these genes were developed. Therefore, through the novel compounds, the present invention showed that antifungal agents having a novel mechanism, i.e. inhibiting the process that transports GPI-anchored proteins to the cell wall, could be achieved.Type: ApplicationFiled: April 15, 2008Publication date: April 16, 2009Applicant: EISAI R&D MANAGEMENT CO., LTDInventors: Kappei Tsukahara, Katsura Hata, Koji Sagane, Kazutaka Nakamoto, Mamiko Tsuchiya, Naoaki Watanabe, Fuminori Ohba, Itaru Tsukada, Norihiro Ueda, Keigo Tanaka, Junko Kai
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Publication number: 20080261272Abstract: A reporter system reflecting the transport process that transports GPI-anchored proteins to the cell wall was constructed and compounds inhibiting this process were discovered. Further, genes conferring resistance to the above compounds were identified and methods of screening for compounds that inhibit the activity of the proteins encoded by these genes were developed. Therefore, through the novel compounds, the present invention showed that antifungal agents having a novel mechanism, i.e. inhibiting the process that transports GPI-anchored proteins to the cell wall, could be achieved.Type: ApplicationFiled: April 15, 2008Publication date: October 23, 2008Applicant: EISAI R&D MANAGEMENT CO., LTDInventors: Kappei Tsukahara, Katsura Hata, Koji Sagane, Kazutaka Nakamoto, Mamiko Tsuchiya, Naoaki Watanabe, Fuminori Ohba, Itaru Tsukada, Norihiro Ueda, Keigo Tanaka, Junko Kai
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Publication number: 20080166765Abstract: A reporter system reflecting the transport process that transports GPI-anchored proteins to the cell wall was constructed and compounds inhibiting this process were discovered. Further, fungal genes conferring resistance to the above compounds were identified and methods of screening for compounds that inhibit the activity of the proteins encoded by these genes were developed. These genes encode proteins participating in fungal cell wall synthesis. Therefore, through the novel compounds, the present invention showed that antifungal agents having a novel mechanism, i.e. inhibiting the process that transports GPI-anchored proteins to the cell wall, could be achieved.Type: ApplicationFiled: December 17, 2007Publication date: July 10, 2008Applicant: Eisai R&D Management, Ltd.Inventors: Kappei Tsukahara, Katsura Hata, Koji Sagane, Kazutaka Nakamoto, Mamiko Tsuchiya, Naoaki Watanabe, Fuminori Oba, Itaru Tsukada, Norihiro Ueda, Keigo Tanaka, Junko Kai
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Patent number: 7375204Abstract: A reporter system reflecting the transport process that transports GPI-anchored proteins to the cell wall was constructed and compounds inhibiting this process were discovered. Further, fungal genes conferring resistance to the above compounds were identified and methods of screening for compounds that inhibit the activity of the proteins encoded by these genes were developed. These genes encode proteins participating in fungal cell wall synthesis. Therefore, through the novel compounds, the present invention showed that antifungal agents having a novel mechanism, i.e. inhibiting the process that transports GPI-anchored proteins to the cell wall, could be achieved.Type: GrantFiled: April 21, 2006Date of Patent: May 20, 2008Assignee: Eisai R&D Management Co., Ltd.Inventors: Kappei Tsukahara, Katsura Hata, Koji Sagane, Kazutaka Nakamoto, Mamiko Tsuchiya, Naoaki Watanabe, Fuminori Ohba, Itaru Tsukada, Norihiro Ueda, Keigo Tanaka, Junko Kai
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Publication number: 20060240429Abstract: The present invention enables screening for compounds that inhibit the transport of GPI-anchored proteins to fungal cell walls, using a simple assay for transacylation to GlcN-PI using membrane fraction expressing GWT1 protein. New antifungal agents can be created that inhibit the synthesis of fungal cell walls and also inhibit adhesion to host cells by inhibiting the transport of GPI-anchored proteins to fungal cell walls.Type: ApplicationFiled: November 21, 2003Publication date: October 26, 2006Inventors: Kappei Tsukahara, Mamiko Tsuchiya, Yoshifumi Jigami, Kenichi Nakayama, Mariko Umemura, Michiyo Okamoto
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Publication number: 20060234283Abstract: A reporter system reflecting the transport process that transports GPI-anchored proteins to the cell wall was constructed and compounds inhibiting this process were discovered. Further, genes conferring resistance to the above compounds were identified and methods of screening for compounds that inhibit the activity of the proteins encoded by these genes were developed. Therefore, through the novel compounds, the present invention showed that antifungal agents having a novel mechanism, i.e. inhibiting the process that transports GPI-anchored proteins to the cell wall, could be achieved.Type: ApplicationFiled: April 21, 2006Publication date: October 19, 2006Applicant: Eisai Co., Ltd.Inventors: Kappei Tsukahara, Katsura Hata, Koji Sagane, Kazutaka Nakamoto, Mamiko Tsuchiya, Naoaki Watanabe, Fuminori Ohba, Itaru Tsukada, Norihiro Ueda, Keigo Tanaka, Junko Kai
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Publication number: 20060234349Abstract: A reporter system reflecting the transport process that transports GPI-anchored proteins to the cell wall was constructed and compounds inhibiting this process were discovered. Further, genes conferring resistance to the above compounds were identified and methods of screening for compounds that inhibit the activity of the proteins encoded by these genes were developed. Therefore, through the novel compounds, the present invention showed that antifungal agents having a novel mechanism, i.e. inhibiting the process that transports GPI-anchored proteins to the cell wall, could be achieved.Type: ApplicationFiled: April 21, 2006Publication date: October 19, 2006Applicant: Eisai Co., Ltd.Inventors: Kappei Tsukahara, Katsura Hata, Koji Sagane, Kazutaka Nakamoto, Mamiko Tsuchiya, Naoaki Watanabe, Fuminori Ohba, Itaru Tsukada, Norihiro Ueda, Keigo Tanaka, Junko Kai
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Publication number: 20040038239Abstract: A reporter system reflecting the transport process that transports GPI-anchored proteins to the cell wall was constructed and compounds inhibiting this process were discovered. Further, genes conferring resistance to the above compounds were identified and methods of screening for compounds that inhibit the activity of the proteins encoded by these genes were developed.Type: ApplicationFiled: May 14, 2003Publication date: February 26, 2004Inventors: Kappei Tsukahara, Katsura Hata, Koji Sagane, Kazutaka Nakamoto, Mamiko Tsuchiya, Naoaki Watanabe, Fuminori Oba, Itaru Tsukada, Norihiro Ueda, Keigo Tanaka, Junko Kai