Patents by Inventor Manuel KAULICH

Manuel KAULICH has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Publication number: 20250043271
    Abstract: The present invention pertains to a novel method for the generation of highly diverse RNA expressing vectors and vector libraries for use in targeted gene knock out, knock down and genome modification approaches. The invention pertains to a method for generating such higher order libraries without the need of classical cloning technologies. This is particularly useful for libraries based on large vectors wherein a sequence cannot be easily mutated with classical mutagenesis methods. The vectors and libraries generated according to the methods of the invention are in particular for RNA assisted silencing technologies such as RNA interference, and for targeted genome editing using the clustered regularly interspaced short palindromic repeats (CRISPR)/Cas system or similar RNA/DNA-encoded gene perturbation systems which use small guide RNAs to target the CRISPR complex to a specific genomic sequence. The invention provides also kits comprising the materials for performing the methods of the invention.
    Type: Application
    Filed: August 14, 2024
    Publication date: February 6, 2025
    Applicant: Johann Wolfgang Goethe-Universität Frankfurt am Main
    Inventors: Manuel KAULICH, Andreas ERNST, Martin WEGNER, Valentina DIEHL, Rahel DE BRUYN, Svenja WIECHMANN
  • Patent number: 12091656
    Abstract: The present invention pertains to a novel method for the generation of highly diverse RNA expressing vectors and vector libraries for use in targeted gene knock out, knock down and genome modification approaches. The invention pertains to a method for generating such higher order libraries without the need of classical cloning technologies. This is particularly useful for libraries based on large vectors wherein a sequence cannot be easily mutated with classical mutagenesis methods. The vectors and libraries generated according to the methods of the invention are in particular for RNA assisted silencing technologies such as RNA interference, and for targeted genome editing using the clustered regularly interspaced short palindromic repeats (CRISPR)/Cas system or similar RNA/DNA-encoded gene perturbation systems which use small guide RNAs to target the CRISPR complex to a specific genomic sequence. The invention provides also kits comprising the materials for performing the methods of the invention.
    Type: Grant
    Filed: December 27, 2017
    Date of Patent: September 17, 2024
    Assignee: Johann Wolfgang Goethe-Universität Frankfur
    Inventors: Manuel Kaulich, Andreas Ernst, Martin Wegner, Valentina Diehl, Rahel De Bruyn, Svenja Wiechmann
  • Patent number: 12023353
    Abstract: The present invention pertains to methods and compounds useful in a therapy involving the administration of immune cells to a patient. The method of the invention involves the modification of cells of the immune system with agonists or antagonists of immune regulators such as Interleukin-10 (IL-10) or IL-6, in order to enhance and improve the immunological potential of the immune cells for therapy. Cells modified according to the method of the invention can be administered to a patient to support a treatment of proliferative diseases such as cancer or autoimmune disorders.
    Type: Grant
    Filed: October 18, 2018
    Date of Patent: July 2, 2024
    Assignee: Chemotherapeutisches Forschungsinstitut Georg-Speyer-Haus
    Inventors: Winfried Wels, Anja Waldmann, Congcong Zhang, Manuel Kaulich
  • Publication number: 20210261954
    Abstract: The present invention pertains to a novel method for the generation of a vector construct suitable for gene editing applications which comprises a fixed pair of predetermined expressible guide RNA (gRNA) sequences. The method of the invention allows for an easy construction of such vectors and provides in addition thereto vector libraries for the expression of fixed pairs of gRNAs. The vectors of the invention may be advantageously used to cut out larger genomic DNA sequences, or alternatively, to simultaneously introduce mutations in the genome without a loss or larger genomic sequences. Hence, the system of the invention provides for many molecular genetic approaches for genome alteration.
    Type: Application
    Filed: June 11, 2019
    Publication date: August 26, 2021
    Applicant: Johann Wolfgang Goethe-Universitat Frankfurt am Main
    Inventors: Manuel Kaulich, Ivan Dikic, Martin Wegner, Yves Matthess, Koraljka Husnjak
  • Publication number: 20210128615
    Abstract: The present invention pertains to methods and compounds useful in a therapy involving the administration of immune cells to a patient. The method of the invention involves the modification of cells of the immune system with agonists or antagonists of immune regulators such as Interleukin-10 (IL-10) or IL-6, in order to enhance and improve the immunological potential of the immune cells for therapy. Cells modified according to the method of the invention can be administered to a patient to support a treatment of proliferative diseases such as cancer or autoimmune disorders.
    Type: Application
    Filed: October 18, 2018
    Publication date: May 6, 2021
    Inventors: Winfried Wels, Anja Waldmann, Congcong Zhang, Manuel Kaulich
  • Publication number: 20190330616
    Abstract: The present invention pertains to a novel method for the generation of highly diverse RNA expressing vectors and vector libraries for use in targeted gene knock out, knock down and genome modification approaches. The invention pertains to a method for generating such higher order libraries without the need of classical cloning technologies. This is particularly useful for libraries based on large vectors wherein a sequence cannot be easily mutated with classical mutagenesis methods. The vectors and libraries generated according to the methods of the invention are in particular for RNA assisted silencing technologies such as RNA interference, and for targeted genome editing using the clustered regularly interspaced short palindromic repeats (CRISPR)/Cas system or similar RNA/DNA-encoded gene perturbation systems which use small guide RNAs to target the CRISPR complex to a specific genomic sequence. The invention provides also kits comprising the materials for performing the methods of the invention.
    Type: Application
    Filed: December 27, 2017
    Publication date: October 31, 2019
    Inventors: Manuel KAULICH, Andreas ERNST, Martin WEGNER, Valentina DIEHL, Rahel DE BRUYN, Svenja WIECHMANN