Patents by Inventor Marcel P. Bruchez
Marcel P. Bruchez has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Publication number: 20210187262Abstract: Provided herein are devices and methods used to produce tattoo biosensors that are based on spatially controlled intracutaneous gene delivery of optical reporters driven by specific transcription factor pathways for a given cytokine or other analyte. The biosensors can be specific to a given analyte, or more generically represent the convergence of several cytokines into commonly shared intracellular transcription factor pathways. These biosensors can be delivered as an array in order to monitor multiple cytokines. Biosensor redeployment can enable chronic monitoring from months to years. The tattooed biosensor array of the present invention includes endogenous reporter cells, naturally tuned to each patient's own biology and can be used to reliably measure the state of a patient in real-time.Type: ApplicationFiled: December 23, 2020Publication date: June 24, 2021Inventors: O. Burak Ozdoganlar, Marcel P. Bruchez, Phil G. Campbell, Jonathan W. Jarvik, Louis Falo, Geza Erdos
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Patent number: 10946098Abstract: Provided herein is a two-component photosensitizer, which demonstrated robust and selective killing effects for transfected HEK cells and affibody targeted A431 cancer cells when exposed to near infrared light excitation. Free MG2I is a pure and stable fluorogen; it is easy to synthesize and modify, and has no toxicity to cells. Unlike conventional photosensitizers, the dye and FAP itself has no photosensitizing effect until they are bound. Also unlike other activation methods, the activation step is achieved by adding the fluorogen, not the presence of the targeted molecule, requiring an ‘active’ activation instead of a ‘passive’ activation. This method offers the ability to locally switch-on and selective generation of singlet oxygen at the target site and can be used for a wide variety of molecular targets.Type: GrantFiled: September 12, 2019Date of Patent: March 16, 2021Assignee: Carnegie Mellon UniversityInventors: Marcel P. Bruchez, Jianjun He, Yi Wang
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Patent number: 10894151Abstract: Provided herein are devices and methods used to produce tattoo biosensors that are based on spatially controlled intracutaneous gene delivery of optical reporters driven by specific transcription factor pathways for a given cytokine or other analyte. The biosensors can be specific to a given analyte, or more generically represent the convergence of several cytokines into commonly shared intracellular transcription factor pathways. These biosensors can be delivered as an array in order to monitor multiple cytokines. Biosensor redeployment can enable chronic monitoring from months to years. The tattooed biosensor array of the present invention includes endogenous reporter cells, naturally tuned to each patient's own biology and can be used to reliably measure the state of a patient in real-time.Type: GrantFiled: April 22, 2016Date of Patent: January 19, 2021Assignees: Carnegie Mellon University, University of Pittsburgh—Of the Commonwealth System of Higher EducationInventors: O. Burak Ozdoganlar, Marcel P. Bruchez, Phil G. Campbell, Jonathan W. Jarvik, Louis Falo, Geza Erdos
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Publication number: 20200078460Abstract: Provided herein is a two-component photosensitizer, which demonstrated robust and selective killing effects for transfected HEK cells and affibody targeted A431 cancer cells when exposed to near infrared light excitation. Free MG2I is a pure and stable fluorogen; it is easy to synthesize and modify, and has no toxicity to cells. Unlike conventional photosensitizers, the dye and FAP itself has no photosensitizing effect until they are bound. Also unlike other activation methods, the activation step is achieved by adding the fluorogen, not the presence of the targeted molecule, requiring an ‘active’ activation instead of a ‘passive’ activation. This method offers the ability to locally switch-on and selective generation of singlet oxygen at the target site and can be used for a wide variety of molecular targets.Type: ApplicationFiled: September 12, 2019Publication date: March 12, 2020Inventors: Marcel P. Bruchez, Jianjun He, Yi Wang
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Patent number: 10434177Abstract: Provided herein is a two-component photosensitizer, which demonstrated robust and selective killing effects for transfected HEK cells and affibody targeted A431 cancer cells when exposed to near infrared light excitation. Free MG2I is a pure and stable fluorogen; it is easy to synthesize and modify, and has no toxicity to cells. Unlike conventional photosensitizers, the dye and FAP itself has no photosensitizing effect until they are bound. Also unlike other activation methods, the activation step is achieved by adding the fluorogen, not the presence of the targeted molecule, requiring an ‘active’ activation instead of a ‘passive’ activation. This method offers the ability to locally switch-on and selective generation of singlet oxygen at the target site and can be used for a wide variety of molecular targets.Type: GrantFiled: November 17, 2015Date of Patent: October 8, 2019Assignee: Carnegie Mellon UniversityInventors: Marcel P. Bruchez, Jianjun He, Yi Wang
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Publication number: 20180280510Abstract: Provided herein is a two-component photosensitizer, which demonstrated robust and selective killing effects for transfected HEK cells and affibody targeted A431 cancer cells when exposed to near infrared light excitation. Free MG2I is a pure and stable fluorogen; it is easy to synthesize and modify, and has no toxicity to cells. Unlike conventional photosensitizers, the dye and FAP itself has no photosensitizing effect until they are bound. Also unlike other activation methods, the activation step is achieved by adding the fluorogen, not the presence of the targeted molecule, requiring an ‘active’ activation instead of a ‘passive’ activation. This method offers the ability to locally switch-on and selective generation of singlet oxygen at the target site and can be used for a wide variety of molecular targets.Type: ApplicationFiled: November 17, 2015Publication date: October 4, 2018Inventors: Marcel P. Bruchez, Jianjun He, Yi Wang
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Patent number: 9995679Abstract: Biosensor comprising an activatable acceptor fluorogen linked via a linker to a donor which transfers energy to the fluorogen on detecting an analyte wherein the fluorogen component reacts and a 100 fold increase in intensity results when the fluorogen interacts non-covalently with an activator e.g. fluorogen activator peptide.Type: GrantFiled: May 25, 2011Date of Patent: June 12, 2018Assignee: Carnegie Mellon UniversityInventors: Alan Waggoner, Marcel P. Bruchez, Brigitte F. Schmidt, Subhasish K. Chakraborty
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Publication number: 20180119077Abstract: Provided herein are devices and methods used to produce tattoo biosensors that are based on spatially controlled intracutaneous gene delivery of optical reporters driven by specific transcription factor pathways for a given cytokine or other analyte. The biosensors can be specific to a given analyte, or more generically represent the convergence of several cytokines into commonly shared intracellular transcription factor pathways. These biosensors can be delivered as an array in order to monitor multiple cytokines. Biosensor redeployment can enable chronic monitoring from months to years. The tattooed biosensor array of the present invention includes endogenous reporter cells, naturally tuned to each patient's own biology and can be used to reliably measure the state of a patient in real-time.Type: ApplicationFiled: April 22, 2016Publication date: May 3, 2018Inventors: O. Burak Ozdoganlar, Marcel P. Bruchez, Phil G. Campbell, Jonathan W. Jarvik, Louis Falo, Geza Erdos
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Patent number: 9688743Abstract: Provided are biosensors, compositions comprising biosensors, and methods of using biosensors in living cells and organisms. The biosensors are able to be selectively targeted to certain regions or structures within a cell. The biosensors may provide a signal when the biosensor is targeted and/or in response to a property of the cell or organism such as membrane potential, ion concentration or enzyme activity.Type: GrantFiled: January 2, 2014Date of Patent: June 27, 2017Assignee: CARNEGIE MELLON UNIVERSITYInventors: Brigitte F. Schmidt, Christopher S. Szent-Gyorgyi, Alan S. Waggoner, Peter B. Berget, Marcel P. Bruchez, Jonathan W. Jarvik
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Patent number: 9249306Abstract: The present invention presents designs for high extinction quenched “dyedrons” that can be activated by conversion of a single acceptor/quencher in the molecular assembly to a fluorescent state. The quencher is activated by noncovalent binding to a unique complementary expressible fluorogen activating peptide (FAP). In this way, the quencher serves as the homogeneous switch, receiving energy efficiently from each of the donor molecules of the dendronic antenna, and releasing it as fluorescence only when activated by binding. The sum of the extinction of the multiple dyes on the antenna will provide dramatic enhancements in the effective brightness of the probe in standard imaging systems. This approach provides a set of probes with exceptional brightness, specifically targeted to an expressed tag that activates the fluorescence of the dyedron.Type: GrantFiled: February 16, 2010Date of Patent: February 2, 2016Assignee: Carnegie Mellon UniversityInventors: Marcel P. Bruchez, Lauren A. Ernst, James Fitzpatrick, Chris Szent-Gyorgyi, Brigitte F. Schmidt, Alan Waggoner
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Patent number: 8993349Abstract: Semiconductor nanoparticle complexes comprising semiconductor nanoparticles in association with cationic polymers are described. Also described are methods for enhancing the transport of semiconductor nanoparticles across biological membranes to provide encoded cells. The methods are particularly useful in multiplex settings where a plurality of encoded cells are to be assayed. Kits comprising reagents for performing such methods are also provided.Type: GrantFiled: October 15, 2010Date of Patent: March 31, 2015Assignee: Life Technologies CorporationInventors: Marcel P. Bruchez, R. Hugh Daniels, Jennifer Dias, Larry C. Mattheakis, Hongjian Liu, Aquanette M. Burt, Berndt Christoffer Lagerholm, Danith H. Ly
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Publication number: 20140193830Abstract: Provided are biosensors, compositions comprising biosensors, and methods of using biosensors in living cells and organisms. The biosensors are able to be selectively targeted to certain regions or structures within a cell. The biosensors may provide a signal when the biosensor is targeted and/or in response to a property of the cell or organism such as membrane potential, ion concentration or enzyme activity.Type: ApplicationFiled: January 2, 2014Publication date: July 10, 2014Inventors: Brigitte F. Schmidt, Christopher S. Szent-Gyorgyi, Alan S. Waggoner, Peter B. Berget, Marcel P. Bruchez, Jonathan W. Jarvik
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Patent number: 8664364Abstract: Provided are biosensors, compositions comprising biosensors, and methods of using biosensors in living cells and organisms. The biosensors are able to be selectively targeted to certain regions or structures within a cell. The biosensors may provide a signal when the biosensor is targeted and/or in response to a property of the cell or organism such as membrane potential, ion concentration or enzyme activity.Type: GrantFiled: January 24, 2008Date of Patent: March 4, 2014Assignee: Carnegie Mellon UniversityInventors: Brigitte F. Schmidt, Christopher S. Szent-Gyorgyi, Alan S. Waggoner, Peter B. Berget, Marcel P. Bruchez, Jonathan W. Jarvik
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Publication number: 20130244891Abstract: Biosensor comprising an activatable acceptor fluorogen linked via a linker to a donor which transfers energy to the fluorogen on detecting an analyte wherein the fluorogen component reacts and a 100 fold increase in intensity results when the fluorogen interacts non-covalently with an activator e.g. fluorogen activator peptide.Type: ApplicationFiled: May 25, 2011Publication date: September 19, 2013Applicant: CARNEGIE MELLON UNIVERSITYInventors: Alan Waggoner, Marcel P. Bruchez, Brigitte F. Schmidt, Subhasish K. Chakraborty
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Publication number: 20120252018Abstract: The use of semiconductor nanocrystals as detectable labels in various chemical and biological applications is disclosed. The methods find use for detecting a single analyte, as well as multiple analytes by using more than one semiconductor nanocrystal as a detectable label, each of which emits at a distinct wavelength.Type: ApplicationFiled: April 9, 2012Publication date: October 4, 2012Applicant: Life Technologies CorporationInventors: Marcel P. Bruchez, R. Hugh Daniels, Stephen A. Empedocles, Vince E. Phillips, Edith Y. Wong, Donald A. Zehnder
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Publication number: 20120058494Abstract: The present invention presents designs for high extinction quenched “dyedrons” that can be activated by conversion of a single acceptor/quencher in the molecular assembly to a fluorescent state. The quencher is activated by noncovalent binding to a unique complementary expressible fluorogen activating peptide (FAP). In this way, the quencher serves as the homogeneous switch, receiving energy efficiently from each of the donor molecules of the dendronic antenna, and releasing it as fluorescence only when activated by binding. The sum of the extinction of the multiple dyes on the antenna will provide dramatic enhancements in the effective brightness of the probe in standard imaging systems. This approach provides a set of probes with exceptional brightness, specifically targeted to an expressed tag that activates the fluorescence of the dyedron.Type: ApplicationFiled: February 16, 2010Publication date: March 8, 2012Applicant: CARNEGIE MELLON UNIVERSITYInventors: Marcel P. Bruchez, Lauren A. Ernst, James Fitzpatrick, Chris Szent-Gyorgyi, Brigitte F. Schmidt, Alan Waggoner
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Publication number: 20110159519Abstract: Provided are biosensors, compositions comprising biosensors, and methods of using biosensors in living cells and organisms. The biosensors are able to be selectively targeted to certain regions or structures within a cell. The biosensors may provide a signal when the biosensor is targeted and/or in response to a property of the cell or organism such as membrane potential, ion concentration or enzyme activity.Type: ApplicationFiled: January 24, 2008Publication date: June 30, 2011Applicant: Carnegie Mellon UniversityInventors: Brigitte F. Schmidt, Christopher S. Szent-Gyorgyi, Alan S. Waggoner, Peter B. Berget, Marcel P. Bruchez, Johathan W. Jarvik
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Publication number: 20110136139Abstract: Semiconductor nanoparticle complexes comprising semiconductor nanoparticles in association with cationic polymers are described. Also described are methods for enhancing the transport of semiconductor nanoparticles across biological membranes to provide encoded cells. The methods are particularly useful in multiplex settings where a plurality of encoded cells are to be assayed. Kits comprising reagents for performing such methods are also provided.Type: ApplicationFiled: October 15, 2010Publication date: June 9, 2011Applicant: LIFE TECHNOLOGIES CORPORATIONInventors: MARCEL P. BRUCHEZ, R. HUGH DANIELS, JENNIFER DIAS, LARRY C. MATTHEAKIS, HONGJIAN LIU, AQUANETTE M. BURT, BERNDT CHRISTOFFER LAGERHOLM, DANITH H. LY
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Publication number: 20100190657Abstract: Methods for assaying a sample for a probe polynucleotide are provided. The methods comprise forming a complex between a target on a substrate, the probe polynucleotide that binds to the target, and a conjugate comprising a semiconductor nanocrystal that binds to the probe polynucleotide by way of a tag sequence on the probe polynucleotide. The complex is formed when the probe polynucleotide is present in the sample. The methods are useful in any technique in which the detection of a target that can bind to a probe polynucleotide is desired, for example in fluorescence in situ hybridization. The methods are particularly useful in multiplex settings such as hybridization to microarrays where a plurality of targets are present. Assay complexes produced by such methods and kits useful for performing such methods are also provided.Type: ApplicationFiled: October 22, 2009Publication date: July 29, 2010Applicant: LIFE TECHNOLOGIES CORPORATIONInventors: Michael BITTNER, Edith Y. Wong, Marcel P. Bruchez, JR.
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Publication number: 20090176221Abstract: The use of semiconductor nanocrystals as detectable labels in various chemical and biological applications is disclosed. The methods find use for detecting a single analyte, as well as multiple analytes by using more than one semiconductor nanocrystal as a detectable label, each of which emits at a distinct wavelength.Type: ApplicationFiled: May 19, 2008Publication date: July 9, 2009Applicant: INVITROGEN CORPORATIONInventors: Marcel P. Bruchez, R. Hugh Daniels, Stephen A. Empedocles, Vince E. Phillips, Edith Y. Wong, Donald A. Zehnder