Patents by Inventor Marcus Landthaler

Marcus Landthaler has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Method for Identifying RNA Segments Bound by RNA-Binding Proteins or Ribonucleoprotein Complexes
    Publication number: 20150353923
    Abstract: The present invention relates to a method for identifying a binding site on an RNA transcript, wherein the binding site binds to one or more binding moieties. The method includes, among other things, introducing a photoreactive nucleoside into living cells wherein the living cells incorporate the photoreactive nucleoside into RNA transcripts during transcription thereby producing modified RNA transcripts; reverse transcribing the RNA of isolated cross-linked segments thereby generating cDNA transcripts with one mutation wherein the photoreactive nucleoside is transcribed to a mismatched deoxynucleoside; amplifying the cDNA transcripts thereby generating amplicons; and analyzing the sequences of the amplicons aligned against the reference sequence so as to identify the binding site, wherein the sequences of each amplicon having a mutation resulting from the introduction of the photoreactive nucleoside is considered to be a valid amplicon comprising at least a portion of a binding site on the RNA transcript.
    Type: Application
    Filed: August 22, 2014
    Publication date: December 10, 2015
    Inventors: Marcus Landthaler, Manuel Ascano, JR., Markus Hafner, Thomas Tuschl, Mohsen Khorshid, Lukas Burger, Mihaela Zavolan
  • Methods for Identifying RNA Segments Bound by RNA-Binding Proteins or Ribonucleoprotein Complexes
    Publication number: 20110287412
    Abstract: The present invention relates to a method for identifying a binding site on an RNA transcript, wherein the binding site binds to one or more binding moieties. The method includes, among other things, introducing a photoreactive nucleoside into living cells wherein the living cells incorporate the photoreactive nucleoside into RNA transcripts during transcription thereby producing modified RNA transcripts; reverse transcribing the RNA of isolated cross-linked segments thereby generating cDNA transcripts with one mutation wherein the photoreactive nucleoside is transcribed to a mismatched deoxynucleoside; amplifying the cDNA transcripts thereby generating amplicons; and analyzing the sequences of the amplicons aligned against the reference sequence so as to identify the binding site, wherein the sequences of each amplicon having a mutation resulting from the introduction of the photoreactive nucleoside is considered to be a valid amplicon comprising at least a portion of a binding site on the RNA transcript.
    Type: Application
    Filed: July 28, 2009
    Publication date: November 24, 2011
    Applicant: ROCKEFELLER UNIVERSITY
    Inventors: Marcus Landthaler, Manuel Ascano, JR., Markus Hafner, Thomas Tuschl, Mohsen Khorshid, Lukas Burger, Mihaela Zavolan