Abstract: A composite article is provided having covalently reactive particles incorporated in a continuous, porous matrix. The reactive particles have surfaces of covalently reactive functional groups capable of directly forming covalent chemical bonds with ligands without need for an intermediate activation step. An adduct composite article is also provided comprising a continuous, porous matrix and derivatized particles dispersed therein. The derivatized particles comprise a direct, covalent reaction product of ligand with the covalently reactive particles. Methods of making and using the composite articles and adduct composite articles are also provided. Preferred covalently reactive functional groups are azlactone-functional groups of the formula: ##STR1## wherein: R.sup.1 and R.sup.

Abstract: Azlactone-functional polymer supports are useful reactive supports for the attachment of functional materials to provide novel adduct beads. The adduct beads are useful as complexing agents, catalysts, polymeric reagents, chromatographic supports, and as enzyme- or other biologically active supports. Novel carboxylate-functional polymer beads, are intermediates in the preparation of the azlactone-functional beads.Azlactone-functional supports have units of the formula: ##STR1## wherein R.sup.1 is H or CH.sub.3,R.sup.2 and R.sup.3 independently can be an alkyl group having 1 to 14 carbon atoms, a cycloalkyl group having 3 to 14 carbon atoms, an aryl group having 5 to 12 ring atoms, an arenyl group having 6 to 26 carbon and 0 to 3 S, N, and nonperoxidic O heteroatoms, or R.sup.2 and R.sup.3 taken together with the carbon to which they are joined can form a carbocyclic ring containing 4 to 12 ring atoms, andn is an integer 0 or 1, the azlactone functional supports having 0.

Abstract: Azlactone-functional polymer supports are useful reactive supports for the attachment of functional materials to provide novel adduct beads. The adduct beads are useful as complexing agents, catalysts, polymeric reagents, chromatographic supports, and as enzyme- or other biologically active supports. Novel carboxylate-functional polymer beads, are intermediates in the preparation of the azlactone-functional beads.Azlactone-functional supports have units of the formula: ##STR1## wherein R.sup.1 is H or CH.sub.3,R.sup.2 and R.sup.3 independently can be an alkyl group having 1 to 14 carbon atoms, a cycloalkyl group having 3 to 14 carbon atoms, an aryl group having 5 to 12 ring atoms, an arenyl group having 6 to 26 carbon and 0 to 3 S, N, and nonperoxidic O heteroatoms, or R.sup.2 and R.sup.3 taken together with the carbon to which they are joined can form a carbocyclic ring containing 4 to 12 ring atoms, andn is an integer 0 or 1,the azlactone functional supports having 0.

Abstract: A biologically active adduct support, prepared from an azlactone-functional polymeric support and biomolecules covalently immobilized thereon, is disclosed. The biomolecules are covalently immobilized in the presence of polyanionic salt using a method which achieves both high density and optimized bound specific biological activity. Preferably, the immobilization occurs in the presence of an azlactone quencher.