Patents by Inventor Maria-Regina Kula

Maria-Regina Kula has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Patent number: 8034562
    Abstract: The invention relates to a process for isolation of plasmid DNA from biomass by means of an aqueous 2-phase system having a polymer component and a salt component, characterized in that the resuspension of the biomass employed, the alkaline lysis of the biomass, the neutralization of the alkaline lysis batch and the separation of the plasmid DNA from the contaminants are carried out in a single reaction vessel (one-pot process) rendered possible in that the neutralization of the alkaline lysis batch is carried out in one and the same container by addition of potassium phosphate and one component of the aqueous 2-phase system is therefore already present. The second component of the aqueous 2-phase system is a PEG having a molecular weight of the mathematical average of about 600 g/mol to 1,000 g/mol.
    Type: Grant
    Filed: May 28, 2004
    Date of Patent: October 11, 2011
    Assignee: QIAGEN GmbH
    Inventors: Markus Müller, Jürgen Hubbuch, Andreas Frerix, Maria-Regina Kula
  • Publication number: 20100009434
    Abstract: The invention relates to a method for stripping undesired nucleic acid components from double-stranded DNA, in particular, super-coiled plasmid DNA. The method according to the invention is characterised by the steps: (a) provision of a mixture containing completely and/or partly double-stranded nucleic acids and optionally single-stranded nucleic acids; (b) resuspension of the mixture from step (a) in an aqueous, low-molarity buffer system with low ionic strength and low buffer effect; (c) adjusting conditions in the mixture from step (b), under which the completely and/or partly double-stranded nucleic acids are denatured; (d) further addition of buffer and a polymer component to the mixture from step (c); (e) incubation of the mixture from step (d) for a time which is sufficient for the formation of an aqueous two-phase system with an upper and lower phase; and (f) removal of the upper phase containing the single-strand nucleic acid and collection of the double-strand nucleic acid from the lower phase.
    Type: Application
    Filed: September 13, 2006
    Publication date: January 14, 2010
    Inventors: Markus Müller, Maria-Regina Kula, Jürgen Hubbuch, Andreas Frerix
  • Patent number: 7491521
    Abstract: The invention relates to a Rhodococcus polynucleotide cluster which contains nucleotide sequences which encode polypeptides having the activity of a nitrile hydratase, of an auxiliary protein P15K which activates this enzyme and of a cobalt transporter, to transformed microorganisms in which the nucleotide sequences encoding these proteins are present in increased quantity, and to the use of the transformed microorganisms for preparing amides from nitriles.
    Type: Grant
    Filed: August 20, 2007
    Date of Patent: February 17, 2009
    Assignee: Evonik Degussa GmbH
    Inventors: Steffen Osswald, Stefan Verseck, Uta Deiting, Christoph Weckbecker, Klaus Huthmacher, Michael Binder, Maria-Regina Kula, Konrad Odendahl
  • Publication number: 20080057549
    Abstract: The invention relates to a Rhodococcus polynucleotide cluster which contains nucleotide sequences which encode polypeptides having the activity of a nitrile hydratase, of an auxiliary protein P15K which activates this enzyme and of a cobalt transporter, to transformed microorganisms in which the nucleotide sequences encoding these proteins are present in increased quantity, and to the use of the transformed microorganisms for preparing amides from nitriles.
    Type: Application
    Filed: August 20, 2007
    Publication date: March 6, 2008
    Applicant: Degussa AG
    Inventors: Steffen Osswald, Stefan Verseck, Uta Deiting, Christoph Weckbecker, Klaus Huthmacher, Michael Binder, Maria-Regina Kula, Konard Odendahl
  • Patent number: 7335492
    Abstract: The present invention relates to isolation and purification of protein in aqueous two-phase systems (ATPS). Specifically, the invention provides processes for partitioning of proteins of interest in ATPS by fusing said proteins to targeting proteins which have the ability of carrying said protein into one of the phases.
    Type: Grant
    Filed: October 19, 2005
    Date of Patent: February 26, 2008
    Assignee: Valtion Teknillinen Tutkimuskeskus
    Inventors: Merja Penttilä, Tiina Nakari-Setälä, Richard Fagerström, Klaus Selber, Maria-Regina Kula, Markus Linder, Folke Tjerneld
  • Patent number: 7288402
    Abstract: The invention relates to a Rhodococcus polynucleotide cluster which contains nucleotide sequences which encode polypeptides having the activity of a nitrile hydratase, of an auxiliary protein P15K which activates this enzyme and of a cobalt transporter, to transformed microorganisms in which the nucleotide sequences encoding these proteins are present in increased quantity, and to the use of the transformed microorganisms for preparing amides from nitriles.
    Type: Grant
    Filed: March 18, 2005
    Date of Patent: October 30, 2007
    Assignee: Degussa AG
    Inventors: Steffen Osswald, Stefan Verseck, Uta Deiting, Christoph Weckbecker, Klaus Huthmacher, Michael Binder, Maria-Regina Kula, Konrad Odendahl
  • Patent number: 7217550
    Abstract: The present invention relates to nucleotide-sugar-synthesizing enzymes (enzymes with nucleotidyltransferase or pyrophosphorylase activity) from nonparasitic protists, to a process for the preparation thereof and to the use thereof for preparing nucleotide-sugars. The enzymes according to the invention make possible or greatly simplify the enzymatic preparation of various nucleotide-sugars on the industrial scale from low-cost precursors. It is possible with the aid of the discovered enzymes to prepare, for example, GDP-fucose, GDP-mannose, UDP-glucose, UDP-glucosamine, UDP-galactose, UDP-galactosamine, UDP-N-acetylglucosamine and UDP-N-acetylgalactosamine in economic quantities.
    Type: Grant
    Filed: May 12, 2003
    Date of Patent: May 15, 2007
    Assignee: Nutrinova, Nutrition Specialties & Food Ingredients, GmbH
    Inventors: Thomas Kiy, Lothar Elling, Maria Regina Kula
  • Publication number: 20060286080
    Abstract: The invention relates to a process for isolation of plasmid DNA from biomass by means of an aqueous 2-phase system having a polymer component and a salt component, characterized in that the resuspension of the biomass employed, the alkaline lysis of the biomass, the neutralization of the alkaline lysis batch and the separation of the plasmid DNA from the contaminants (such as e.g. cell debris, RNA and gDNA) are carried out in a single reaction vessel (one-pot process). According to the invention, this is rendered possible in that the neutralization of the alkaline lysis batch is carried out in one and the same container by addition of potassium phosphate and one component of the aqueous 2-phase system is therefore already present, and in that the second component of the aqueous 2-phase system is a PEG having a molecular weight of the mathematical average of about 600 g/mol to 1,000 g/mol, but is preferably formed from a mixture of PEG 600 and PEG 1000.
    Type: Application
    Filed: May 28, 2004
    Publication date: December 21, 2006
    Inventors: Markus Muller, Jurgen Hubbuch, Andreas Frerix, Maria-Regina Kula
  • Patent number: 7070963
    Abstract: The present invention relates to an amidase enzyme, nucleic acids encoding the amidase, as well as methods of employing the nucleic acids and/or amidase to produce, for example, enantiomericallyenriched compounds such as D-amino acids.
    Type: Grant
    Filed: December 4, 2002
    Date of Patent: July 4, 2006
    Assignee: Degussa AG
    Inventors: Stefan Verseck, Karlheinz Drauz, Andreas Bommarius, Maria-Regina Kula, Lutz Krieg, Heike Slusarczyk, Marion Ansorge-Schumacher
  • Patent number: 7060669
    Abstract: The present invention relates to isolation and purification of proteins in aqueous two-phase systems (ATPS). Specifically the invention provides processes for partitioning of proteins of interest in ATPS by fusing said proteins to targeting proteins which have the ability of carrying said protein into one of the phases.
    Type: Grant
    Filed: March 24, 2000
    Date of Patent: June 13, 2006
    Assignee: Valtion Teknillinen Tutkimuskeskus
    Inventors: Merja Penttilä, Tiina Nakari-Setälä, Richard Fagerström, Klaus Selber, Maria-Regina Kula, Markus Linder, Folke Tjerneld
  • Publication number: 20060084164
    Abstract: The present invention relates to isolation and purification of protein in aqueous two-phase systems (ATPS). Specifically, the invention provides processes for partitioning of proteins of interest in ATPS by fusing said proteins to targeting proteins which have the ability of carrying said protein into one of the phases.
    Type: Application
    Filed: October 19, 2005
    Publication date: April 20, 2006
    Inventors: Merja Penttila, Tiina Nakari-Setala, Richard Fagerstrom, Klaus Selber, Maria-Regina Kula, Markus Linder, Folke Tjerneld
  • Publication number: 20060068467
    Abstract: The invention relates to a Rhodococcus polynucleotide cluster which contains nucleotide sequences which encode polypeptides having the activity of a nitrile hydratase, of an auxiliary protein P15K which activates this enzyme and of a cobalt transporter, to transformed microorganisms in which the nucleotide sequences encoding these proteins are present in increased quantity, and to the use of the transformed microorganisms for preparing amides from nitriles.
    Type: Application
    Filed: March 18, 2005
    Publication date: March 30, 2006
    Inventors: Steffen Osswald, Stefan Verseck, Uta Deiting, Christoph Weckbecker, Klaus Huthmacher, Michael Binder, Maria-Regina Kula, Konrad Odendahl
  • Patent number: 6767725
    Abstract: The invention relates to the use of the N-acetyl amino acid racemase from Amycolatopsis orientalis subspecies lurida for the racemiszation of N-carbamoyl amino acids. This use permits the 100% preparation of optically pure amino acids starting from racemic hydantoins in an enzymatic overall process.
    Type: Grant
    Filed: October 11, 2001
    Date of Patent: July 27, 2004
    Assignee: Degussa AG
    Inventors: Andreas Bommarius, Karlheinz Drauz, Stefan Verseck, Maria-Regina Kula
  • Publication number: 20030207433
    Abstract: The present invention relates to nucleotide-sugar-synthesizing enzymes (enzymes with nucleotidyltransferase or pyrophosphorylase activity) from nonparasitic protists, to a process for the preparation thereof and to the use thereof for preparing nucleotide-sugars. The enzymes according to the invention make possible or greatly simplify the enzymatic preparation of various nucleotide-sugars on the industrial scale from low-cost precursors. It is possible with the aid of the discovered enzymes to prepare, for example, GDP-fucose, GDP-mannose, UDP-glucose, UDP-glucosamine, UDP-galactose, UDP-galactosamine, UDP-N-acetylglucosamine and UDP-N-acetylgalactosamine in economic quantities.
    Type: Application
    Filed: May 12, 2003
    Publication date: November 6, 2003
    Inventors: Thomas Kiy, Lothar Elling, Maria Regina Kula
  • Publication number: 20030186423
    Abstract: The present invention relates to an amidase enzyme, nucleic acids encoding the amidase, as well as methods of employing the nucleic acids and/or amidase to produce, for example, enantiomericallyenriched compounds such as D-amino acids.
    Type: Application
    Filed: December 4, 2002
    Publication date: October 2, 2003
    Applicant: Degussa AG
    Inventors: Stefan Verseck, Karlheinz Drauz, Andreas Bommarius, Maria-Regina Kula, Lutz Krieg, Heike Slusarczyk, Marion Ansorge-Schumacher
  • Publication number: 20030157664
    Abstract: Novel improved enzymes prepared by recombination, especially to rec-FDHs. Directed evolution has made it possible to generate catalytically more active and more stable muteins which can preferably be used in an industrial process for the preparation of e.g. amino acids. The invention further relates to the nucleic acids coding for these enzymes, to vehicles containing these nucleic acids and to advantageous primers for the preparation of the nucleic acids by means of PCR. The invention additionally relates to a process for the preparation of further improved rec-FDHs, and a method of screening more stable and/or more active dehydrogenases is claimed.
    Type: Application
    Filed: September 23, 2002
    Publication date: August 21, 2003
    Applicant: Degussa AG
    Inventors: Heike Slusarczyk, Stephan Felber, Maria-Regina Kula, Martina Pohl
  • Publication number: 20030082752
    Abstract: The invention provides a GDP-mannose-pyrophosphorylase, which is suitable for use in continuous multiple stage processes. The mannose- or mannose-derivative-specific GDP-mannose-prophosphorylase, which can be isolated from microorganisms, has a specific activeity of≧2U/mg, is prepared.
    Type: Application
    Filed: March 19, 2001
    Publication date: May 1, 2003
    Inventors: Jorg Eberhard Ritter, Lothar Elling, Maria-Regina Kula, Stefen Verseck
  • Publication number: 20020106752
    Abstract: The invention relates to the use of the N-acetyl amino acid racemase from Amycolatopsis orientalis subspecies lurida for the racemiszation of N-carbamoyl amino acids. This use permits the 100% preparation of optically pure amino acids starting from racemic hydantoins in an enzymatic overall process.
    Type: Application
    Filed: October 11, 2001
    Publication date: August 8, 2002
    Applicant: DEGUSSA AG
    Inventors: Andreas Bommarius, Karlheinz Drauz, Stefan Verseck, Maria-Regina Kula
  • Patent number: 6372459
    Abstract: The present invention is directed to an N-acetyl amino acid racemase (AAR) as well as the gene coding for it, to a plasmid, vector and microroganism containing the gene. A method of producing enantiomer-enriched amino acids, and derivatives thereof, is also provided.
    Type: Grant
    Filed: July 27, 2000
    Date of Patent: April 16, 2002
    Assignee: Degussa AG
    Inventors: Stefan Verseck, Maria-Regina Kula, Andreas Bommarius, Karlheinz Drauz
  • Patent number: 6333176
    Abstract: A plant peptide amidase from the flavedo of oranges is known; this can be obtained only in small quantities and is seasonal. The proposed new enzymes are microbial peptide amidases which can be obtained from micro-organisms recovered from soil samples in a “double screening” and grown. These microbial peptide amidases are particularly useful for (a) the production of peptides and N-terminal-protected amino acids, (b) racemate splitting of N-protected amino acid amides, (c) obtaining non-proteinogenous D-amino acids, and (d) obtaining new N&agr;-protected D-amino acid amides.
    Type: Grant
    Filed: August 25, 1999
    Date of Patent: December 25, 2001
    Assignee: Degussa Aktiengesellschaft
    Inventors: Ursula Stelkes-Ritter, Maria-Regina Kula, Klaudia Wyzgol, Andreas Bommarius, Michael Schwarm, Karlheinz Drauz