Abstract: The present invention provides a set of oligonucleotides to screen for the presence of targeted Salmonella serotypes in enrichment or to characterize presumptive colonies. The set of oligonucleotides includes at least one set of primers and probe for the detection of Salmonella serotype selected from Newport, Heidelberg, Infantis, and Hadar and to discriminate between. These new markers can be used after the initial screening assay described herein as a discrimination assay to differentiate S. Heidelberg from S. Infantis and S. Hadar from S. Newport.

Abstract: The present invention provides a set of oligonucleotides to screen for the presence of targeted Salmonella serotypes in an enrichment or to characterize presumptive colonies. The set of oligonucleotides includes at least one set of primers and probe for the detection of Salmonella serotype selected from typhimurium, enteritidis, newport, heidelberg, infantis, virchow and Hadar. The set of oligonucleotides may include up to 5 different primer sets and the corresponding probes.

Abstract: The present invention provides a set of oligonucleotides to screen for the presence of targeted Salmonella serotypes in enrichment or to characterize presumptive colonies. The set of oligonucleotides includes at least one set of primers and probe for the detection of Salmonella serotype selected from Newport, Heidelberg, Infantis, and Hadar and to discriminate between. These new markers can be used after the initial screening assay described herein as a discrimination assay to differentiate S. Heidelberg from S. Infantis and S. Hadar from S. Newport.

Abstract: The present invention provides a set of oligonucleotides to screen for the presence of targeted Salmonella serotypes in an enrichment or to characterize presumptive colonies. The set of oligonucleotides includes at least one set of primers and probe for the detection of Salmonella serotype selected from typhimurium, enteritidis, newport, heidelberg, infantis, virchow and Hadar. The set of oligonucleotides may include up to 5 different primer sets and the corresponding probes.

Abstract: The present invention relates to a process to perform a molecular risk assessment (MRA) upon a sample suspected to contain an enterohemorrhagic Escherichia coli (EHEC), comprising the steps: contacting said sample or DNA isolated therefrom with a pair of primers derived from the following target genes stx1, stx2, eae and/or espk; wherein the process is characterized in that if amplification products are detected for each of the target genes in the first step, in a second step said sample or DNA isolated therefrom is contacted with a pair of primers derived from the following target genes nleB, nleH1-2, nleE, ent/espL2, eae subtypes ?, ?, ? and ? and the target genes rfbE (0157), wbdl (0111), wzx (026); ihp1 (0145), wzx (0103); and detecting the presence or the absence of an amplification product for each of the target genes.

Abstract: The present invention relates to a process to perform a molecular risk assessment (MRA) upon a sample suspected to contain an enterohemorrhagic Escherichia coli (EHEC), comprising the steps: contacting said sample or DNA isolated therefrom with a pair of primers derived from the following target genes stx1, stx2, eae and/or espk; wherein the process is characterised in that if amplification products are detected for each of the target genes in the first step, in a second step said sample or DNA isolated therefrom is contacted with a pair of primers derived from the following target genes nleB, nleH1-2, nleE, ent/espL2, eae subtypes ?, ?, ? and ? and the target genes rfbE (0157), wbdl (0111), wzx (026); ihp1 (0145), wzx (0103); and detecting the presence or the absence of an amplification product for each of the target genes.