Patents by Inventor Mark Carle Connelly

Mark Carle Connelly has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Publication number: 20200191793
    Abstract: Methods for the detection, enumeration and analysis of circulating tumor cells expressing insulin-like growth factor-1 receptors (IGF-1R) are disclosed. These methods are useful for cancer screening and staging, development of treatment regimens, and for monitoring for treatment responses, cancer recurrence or the like. Test kits that facilitate the detection, enumeration and analysis of such circulating tumor cells are also provided.
    Type: Application
    Filed: July 8, 2019
    Publication date: June 18, 2020
    Applicants: Janssen Diagnostics, LLC, Pfizer Inc.
    Inventors: Antonio GUALBERTO, Maria Luisa PACCAGNELLA, Carrie L. MELVIN, Madeline I. REPOLLET, David CHIANESE, Mark Carle CONNELLY, Leonardus Wendelinus Mathias Marie TERSTAPPEN
  • Publication number: 20180208998
    Abstract: The invention relates generally to the field of the identification of DNA sequences, genes or chromosomes. Methods and composition to obtain Unique Sequence DNA probes are provided. Compositions comprised of and double stranded DNA containing Unique Sequences from which the repetitive sequences are eliminated according to the method described in this invention. The invention also relates to the preservation of cells that have been identified after immunomagnetic selection and fluorescent labeling in order to further interrogate the cells of interest. Furthermore the invention relates to genetic analysis of cells that have been identified after immunomagnetic selection and fluorescent labeling.
    Type: Application
    Filed: March 23, 2018
    Publication date: July 26, 2018
    Applicant: Menarini Silicon Biosystems, Inc.
    Inventors: John Verrant, Mark Carle CONNELLY, Brad FOULK, Michael T. KAGAN, Joost F. SWENNENHUIS, Leon W.M.M. TERSTAPPEN, Arjan G.J. TIBBE
  • Patent number: 9957571
    Abstract: The invention relates generally to the field of the identification of DNA sequences, genes or chromosomes. Methods and composition to obtain Unique Sequence DNA probes are provided. Compositions comprises of and double stranded DNA containing Unique Sequences from which the repetitive sequences are eliminated according to the method described in this invention. The invention also relates to the preservation of cells that have been identified after immunomagnetic selection and fluorescent labeling in order to further interrogate the cells of interest. Furthermore the invention relates to genetic analysis of cells that have been identified after immunomagnetic selection and fluorescent labeling.
    Type: Grant
    Filed: June 23, 2015
    Date of Patent: May 1, 2018
    Assignee: Menarini Silicon Biosystems, Inc.
    Inventors: John Verrant, Mark Carle Connelly, Brad Foulk, Michael T Kagan, Joost F Swennenhuis, Leon W. M. M. Terstappen, Arjan G. J. Tibbe
  • Publication number: 20180011103
    Abstract: Methods for the detection, enumeration and analysis of circulating tumor cells expressing insulin-like growth factor-1 receptors (IGF-1R) are disclosed. These methods are useful for cancer screening and staging, development of treatment regimens, and for monitoring for treatment responses, cancer recurrence or the like. Test kits that facilitate the detection, enumeration and analysis of such circulating tumor cells are also provided.
    Type: Application
    Filed: July 27, 2017
    Publication date: January 11, 2018
    Applicants: Janssen Diagnostics, LLC, Pfizer Inc.
    Inventors: Antonio GUALBERTO, Maria Luisa PACCAGNELLA, Carrie L. MELVIN, Madeline I. REPOLLET, David CHIANESE, Mark Carle CONNELLY, Leonardus Wendelinus Mathias Marie TERSTAPPEN
  • Patent number: 9618515
    Abstract: The invention includes methods for isolating circulating multiple myeloma cells as well as method of treating patients suspected of having diseases of abnormal plasma cells.
    Type: Grant
    Filed: July 20, 2012
    Date of Patent: April 11, 2017
    Assignee: Janssen Diagnostics, LLC
    Inventors: Steven Gross, Mark Carle Connelly, Galla Chandra Rao, Marielena Mata, Carrie Morano
  • Publication number: 20160046998
    Abstract: The invention relates generally to the field of the identification of DNA sequences, genes or chromosomes. Methods and composition to obtain Unique Sequence DNA probes are provided. Compositions comprises of and double stranded DNA containing Unique Sequences from which the repetitive sequences are eliminated according to the method described in this invention. The invention also relates to the preservation of cells that have been identified after immunomagnetic selection and fluorescent labeling in order to further interrogate the cells of interest. Furthermore the invention relates to genetic analysis of cells that have been identified after immunomagnetic selection and fluorescent labeling.
    Type: Application
    Filed: June 23, 2015
    Publication date: February 18, 2016
    Inventors: John Verrant, Mark Carle Connelly, Brad Foulk, Michael T Kagan, Joost F Swennenhuis, Leon W.M.M. Terstappen, Arjan G.J. Tibbe
  • Patent number: 9134237
    Abstract: A sample of blood containing CTCs, or other cells of interest, is stained with fluorescent markers for image analysis and scanned to identify the presence and location within the cartridge of target cells or subcellular elements. A sample containing desired target cells or subcellular elements is then further processed, in part by photobleaching the sample, so that those same targets may be re-analyzed with additional biomarkers conjugated to the same or different fluorochromes using the same imaging criteria that were used for the initial analysis. The present invention has applications with targets such as circulating epithelial, cells, circulating tumor cells, circulating endothelial cells, leukocytes, lymphocyte subsets, cells containing an organelle or receptor of interest, cellular debris, disrupted cells and their debris, or any other formed element that might be captured and imaged.
    Type: Grant
    Filed: July 29, 2008
    Date of Patent: September 15, 2015
    Assignee: Janssen Diagnotics, LLC
    Inventors: Mark Carle Connelly, Frank Coumans, Steve Gross, Mike Kelly
  • Patent number: 9127302
    Abstract: The invention relates generally to the field of identification of DNA sequences, genes or chromosomes. Methods and composition to obtain Unique Sequence DNA probes are provided. Composition comprises of any double stranded DNA containing Unique Sequences from which the repetitive sequences are eliminated according to the method described in this invention. The invention also relates to the preservation of cells that have been identified after immunomagnetic selection and fluorescent labeling in order to further interrogate the cells of interest. Furthermore the invention relates to genetic analysis of cells that have been identified after immunomagnetic selection and fluorescent labeling.
    Type: Grant
    Filed: September 20, 2006
    Date of Patent: September 8, 2015
    Assignee: Janssen Diagnostics, LLC
    Inventors: John A Verrant, Arjan G. J. Tibbe, Brad Foulk, Joost F. Swennenhuis, Leon W. M. M. Terstappen, Mark Carle Connelly, Michael T. Kagan
  • Publication number: 20150177252
    Abstract: Methods for the detection, enumeration and analysis of circulating tumor cells expressing insulin-like growth factor-1 receptors (IGF-1R) are disclosed. These methods are useful for cancer screening and staging, development of treatment regimens, and for monitoring for treatment responses, cancer recurrence or the like. Test kits that facilitate the detection, enumeration and analysis of such circulating tumor cells are also provided.
    Type: Application
    Filed: December 22, 2014
    Publication date: June 25, 2015
    Inventors: Antonio Gualberto, Maria Luisa Paccagnella, Carrie L. Melvin, Madeline I. Repollet, David Chianese, Mark Carle Connelly, Leonardus Wendelinus Mathias Marie Terstappen
  • Patent number: 8940493
    Abstract: Methods for the detection, enumeration and analysis of circulating tumor cells expressing insulin-like growth factor-1 receptors (IGF-1R) are disclosed. These methods are useful for cancer screening and staging, development of treatment regimens, and for monitoring for treatment responses, cancer recurrence or the like. Test kits that facilitate the detection, enumeration and analysis of such circulating tumor cells are also provided.
    Type: Grant
    Filed: May 29, 2007
    Date of Patent: January 27, 2015
    Assignees: Veridex LLC, Pfizer Inc.
    Inventors: Antonio Gualberto, Maria Luisa Paccagnella, Carrie L. Melvin, Madeline I. Repollet, David Chianese, Mark Carle Connelly, Leonardus Wendelinus Mathias Marie Terstappen
  • Publication number: 20130189675
    Abstract: The invention includes methods for isolating circulating multiple myeloma cells as well as method of treating patients suspected of having diseases of abnormal plasma cells.
    Type: Application
    Filed: July 20, 2012
    Publication date: July 25, 2013
    Inventors: Steven Gross, Mark Carle Connelly, Galla Chandra Rao, Marielena Mata, Carrie Morano
  • Publication number: 20120202189
    Abstract: A novel cytometer system, methods and algorithms which provide a rugged, affordable and easy-to-use device in remote locations. All cells in a biological sample are fluorescently labeled, with target cells also magnetically labeled. Non-magnetically labeled cells are imaged for viability. Labeled sample is placed between two wedge-shaped magnets to selectively move the magnetically labeled cells for observation. A LED illuminates the cells and a CCO camera captures the images of the fluorescent light emitted by the target cells. Image analysis performed with a novel algorithm provides a cell count that can be related to the target cell concentration of the original sample.
    Type: Application
    Filed: April 16, 2008
    Publication date: August 9, 2012
    Inventors: Mark Carle Connelly, Frank A.W. Coumans, Jimmy Page, Galla Chandra Rao, Joseph Jollick, JR.
  • Publication number: 20120094275
    Abstract: A highly sensitive assay is disclosed which combines immunomagnetic enrichment with multiparameter flow cytometric or image cytometry to detect, enumerate and characterize carcinoma cells in the blood. The present invention incorporates the conjugation of different antibodies to the same ferrofluid. This has the effect of making the ferrofluid polyspecific with respect to the antigens that the ferrofluid will bind. The multiple antibodies present on the same ferrofluid do not appear to block or otherwise interfere with each other. Such ferrofluids have the highly desirable effect of being able to bind specifically to more than one type of cell. The assay is especially useful to enable the capture of CTCs that have low EpCAM expression, but high expression of other tumor markers; Accordingly, the assay facilitates the biological characterization and staging of carcinoma cells.
    Type: Application
    Filed: October 13, 2011
    Publication date: April 19, 2012
    Inventors: Galla Chandra Rao, Mark Carle Connelly
  • Patent number: 7901950
    Abstract: Elevated number of Circulating Endothelial Cells (CEC) have been implicated in disease conditions associated with the formation or destruction of blood vessels such as acute coronary syndrome, thrombocytopenic purpura, sickle cell disease, sepsis, lupus, nephrotic syndromes, rejection of organ transplants, surgical trauma and cancer. This invention provides a method for assessing the levels of CEC which vary between different studies using a sensitive enrichment, imaging, and enumberation analysis. CD146 is one of the most specific endothelium-associated cell-surface antigens which can be used in image cytometry. CEC analysis provides an essential tool in prognostic/diagnostic evaluation in the clinic.
    Type: Grant
    Filed: February 2, 2007
    Date of Patent: March 8, 2011
    Assignee: Veridex, LLC
    Inventors: Mark Carle Connelly, Gerald V. Doyle, Galla Chandra Rao, Leon W. M. M. Terstappen
  • Patent number: 7674632
    Abstract: A method and composition for detecting and measuring analytes, such as antibodies, which are capable of binding with certain binding partners such as antigens. A homogenous assay is performed in the presence of free unbound antibodies. Such a homogeneous assay testing for specific antibodies is herein possible by defining of test subsets of microparticles having specific antigens thereon which are capable of binding with specific target antibodies. The microparticle suspension also includes at least two calibration subsets of microparticles having a binding partner thereon with at least two known levels of concentration which is capable of binding with human antibodies for the purpose of assay calibration. A verification subset of microparticles is included with another binding partner thereon at a known concentration, capable of binding with anti-human antibodies. This suspension is incubated with a human sample and then is incubated with a tagging component.
    Type: Grant
    Filed: November 15, 2002
    Date of Patent: March 9, 2010
    Assignee: Zeus Scientific, Inc
    Inventors: Mark Carle Connelly, Mark Kopnitsky
  • Publication number: 20100028915
    Abstract: Methods for the detection, enumeration and analysis of circulating tumor cells expressing insulin-like growth factor-1 receptors (IGF-1R) are disclosed. These methods are useful for cancer screening and staging, development of treatment regimens, and for monitoring for treatment responses, cancer recurrence or the like. Test kits that facilitate the detection, enumeration and analysis of such circulating tumor cells are also provided.
    Type: Application
    Filed: May 29, 2007
    Publication date: February 4, 2010
    Inventors: Antonio Gualberto, Maria Luisa Paccagnella, Carrie L. Melvin, Madeline I. Repollet, David Chianese, Mark Carle Connelly, Leonardus Wendelinus Mathias Marie Terstappen
  • Publication number: 20090191535
    Abstract: A method for assessing cancer in test subjects is described based upon enumeration of circulating endothelial cells and/or disseminated tumor cells in a test subject. This method is used to quantify disseminated tumor cells. Correlations with circulating tumor cells provides prognostic information with high accuracy in assessing the risk of recurrence in patients with primary breast cancer.
    Type: Application
    Filed: December 22, 2007
    Publication date: July 30, 2009
    Inventors: Mark Carle Connelly, Gerald V. Doyle, Galla Chandra Rao, Leon W.M.M. Terstappen
  • Publication number: 20090136946
    Abstract: The CellTracksĀ® System provides a system to enumerate circulating melanoma cells in blood. The system immunomagnetically concentrates epithelial cells, fluorescently labels the cells and identifies and quantifies circulating melanoma cells. The absolute number of circulating melanoma cells detected in the peripheral blood tumor load is, in part, a factor in prediction of survival, time to progression, and response to therapy. Diagnosis and monitoring of melanoma has been limited by the inability to monitor circulating melanoma cells. The present invention provides a method to enumerate circulating melanoma cells in blood samples. Accordingly, this technology provides a means and device for monitoring disease progression in patients with melanoma.
    Type: Application
    Filed: October 20, 2008
    Publication date: May 28, 2009
    Inventors: Mark Carle CONNELLY, Galla Chandra Rao, Leon W.M.M. Terstappen
  • Publication number: 20090029378
    Abstract: A sample of blood containing CTCs, or other cells of interest, is stained with fluorescent markers for image analysis and scanned to identify the presence and location within the cartridge of target cells or subcellular elements. A sample containing desired target cells or subcellular elements is then further processed, in part by photobleaching the sample, so that those same targets may be re-analyzed with additional biomarkers conjugated to the same or different fluorochromes using the same imaging criteria that were used for the initial analysis. The present invention has applications with targets such as circulating epithelial, cells, circulating tumor cells, circulating endothelial cells, leukocytes, lymphocyte subsets, cells containing an organelle or receptor of interest, cellular debris, disrupted cells and their debris, or any other formed element that might be captured and imaged.
    Type: Application
    Filed: July 29, 2008
    Publication date: January 29, 2009
    Inventors: Mark Carle Connelly, Frank Coumans, Steve Gross, Mike Kelly