Patents by Inventor Mark T. Reed

Mark T. Reed has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Publication number: 20130344540
    Abstract: Provided herein are methods of nucleic acid amplification. An exemplary method includes the steps of (a) providing a surface comprising a plurality of patches of primers, (b) providing a plurality of different nucleic acid molecules, (c) contacting the plurality of different nucleic acid molecules with the surface under conditions wherein the nucleic acid molecules bind to primers at only a subset of the patches, (d) amplifying the nucleic acid molecules under conditions to saturate the subset of patches with copies of the nucleic acid molecules, and repeating steps (c) and (d), thereby increasing the number of patches that are saturated with copies of the nucleic acid molecules.
    Type: Application
    Filed: January 10, 2012
    Publication date: December 26, 2013
    Inventors: Mark T. Reed, Mark Pratt
  • Publication number: 20120270305
    Abstract: A fluidic device holder configured to orient a fluidic device. The device holder includes a support structure configured to receive a fluidic device. The support structure includes a base surface that faces in a direction along the Z-axis and is configured to have the fluidic device positioned thereon. The device holder also includes a plurality of reference surfaces facing in respective directions along an XY-plane. The device holder also includes an alignment assembly having an actuator and a movable locator arm that is operatively coupled to the actuator. The locator arm has an engagement end. The actuator moves the locator arm between retracted and biased positions to move the engagement end away from and toward the reference surfaces. The locator arm is configured to hold the fluidic device against the reference surfaces when the locator arm is in the biased position.
    Type: Application
    Filed: October 14, 2011
    Publication date: October 25, 2012
    Applicant: ILLUMINA INC.
    Inventors: MARK T. REED, ERIC WILLIAMSON, BRYAN CRANE, PATRICK LEUNG, DALE BUERMANN, ALEXANDER P. KINDWALL, Frederick ERIE, MARK PRATT, JASON HARRIS, ANDREW JAMES CARSON, Stanley S. HONG, JASON BRYANT, MARK WANG, DREW VERKADE
  • Publication number: 20120195794
    Abstract: A flow cell for use in a microfluidic detection system is provided. The flow cell includes a flow cell body having a channel that is configured to convey a solution through the flow cell body. The flow cell also includes a bottom surface and a top surface. The bottom surface is configured to be removably held by the detection system, and the top surface is transparent and permits light to pass therethrough. The flow cell body also includes fluidic inlet and outlet ports that are in fluid communication with the channel. A pump cavity is also provided in the flow cell body. The pump cavity fluidly communicates with, and is interposed between, an end of the channel and one of the fluidic inlet and outlet ports. An electroosmotic (EO) pump is held in the pump cavity. The EO pump induces flow of the solution through the EO pump and channel between the fluidic inlet and outlet ports.
    Type: Application
    Filed: April 9, 2012
    Publication date: August 2, 2012
    Applicant: Illumina, Inc
    Inventors: Michal Lebl, Dale Buermann, Mark T. Reed, David L. Heiner, Alexander Triener
  • Patent number: 8173080
    Abstract: A flows cell for use in a microfluidic detection system is provided. The flow cell includes a flows cell body having a channel that is configured to convey a solution through the flows cell body. The flow cell also includes a bottom surface and a top surface. The bottom surface is configured to be removably held by the detection system, and the top surface is transparent and permits light to pass therethrough. The flow cell body also includes fluidic inlet and outlet ports that are in fluid communication with the channel. A pump cavity is also provided in the flow cell body. The pump cavity fluidly communicates with, and is interposed between, an end of the channel and one of the fluidic inlet and outlet ports. An electroosmotic (EO) pump is held in the pump cavity. The EO pump induces flow of the solution through the EO pump and channel between the fluidic inlet and outlet ports.
    Type: Grant
    Filed: February 14, 2008
    Date of Patent: May 8, 2012
    Assignee: Illumina, Inc.
    Inventors: Michal Lebl, Dale Buermann, Mark T. Reed, David L. Heiner, Alexander Triener
  • Publication number: 20110220239
    Abstract: A filling apparatus for filling a microplate. The microplate can comprise a plurality of wells each sized to receive an assay. A substrate can comprise a first surface and an opposing second surface, a first assay input port for receiving the assay disposed on the first surface, a plurality of staging capillaries extending through the substrate, and a first plurality of microfluidic channels fluidly coupling the first assay input port with at least one of the plurality of staging capillaries. Each of the plurality of staging capillaries can comprise an inlet and an outlet and be sized to receive the assay.
    Type: Application
    Filed: March 16, 2011
    Publication date: September 15, 2011
    Applicant: LIFE TECHNOLOGIES CORPORATION
    Inventors: Mark T. REED, Albert L. Carrillo, Ian A. Harding
  • Patent number: 7998435
    Abstract: A filling apparatus for filling a microplate. The microplate having a plurality of wells each sized to receive an assay. The filling apparatus can comprise an output layer having a plurality of capillaries, wherein a first grouping of the capillaries is separated from a second grouping by a hydrophobic feature. Each of the plurality of capillaries can comprise an inlet and an outlet. A funnel assembly can comprise a funnel member sized to receive the assay. The funnel member can comprise an outlet for delivering a fluid bead of the assay along a top surface of the output layer and in fluid communication with each of the plurality of capillaries such that a portion of the fluid bead can be drawn within at least some of the plurality of capillaries in response to capillary force. The funnel assembly and the output layer can be moveable relative to each other between a first position and a second position to draw the fluid bead across the top surface.
    Type: Grant
    Filed: March 30, 2006
    Date of Patent: August 16, 2011
    Assignee: Life Technologies Corporation
    Inventor: Mark T. Reed
  • Publication number: 20110072914
    Abstract: A flows cell for use in a microfluidic detection system is provided. The flow cell includes a flows cell body having a channel that is configured to convey a solution through the flows cell body. The flow cell also includes a bottom surface and a top surface. The bottom surface is configured to be removably held by the detection system and the top surface is transparent and permits light to pass therethrough. The flow cell body also includes fluidic inlet and outlet ports that are in fluid communication with the channel. A pump cavity is also provided in the flow cell body. The pump cavity fluidly communicates with, and is interposed between, an end of the channel and one of the fluidic inlet and outlet ports. An electroosmotic (EO) pump is held in the pump cavity. The EO pump induces flow of the solution through the EO pump and channel between the fluidic inlet and outlet ports.
    Type: Application
    Filed: February 14, 2008
    Publication date: March 31, 2011
    Applicant: ILLUMINA, INC.
    Inventors: Michal Lebl, Dale Buermann, Mark T. Reed, David L. Heiner, Alexander Triener
  • Publication number: 20100221149
    Abstract: A filling apparatus for filling a microplate. The microplate having a plurality of wells each sized to receive an assay. The filling apparatus can comprise an output layer having a plurality of capillaries. Each of the plurality of capillaries can comprises an inlet and an outlet. A funnel assembly can comprise a funnel member sized to receive the assay. The funnel member can comprise an outlet for delivering a fluid bead of the assay along a top surface of the output layer and in fluid communication with each of the plurality of capillaries such that a portion of the fluid bead can be drawn within at least some of the plurality of capillaries in response to capillary force. The funnel assembly and the output layer can be moveable relative to each other between a first position and a second position to draw the fluid bead across the top surface.
    Type: Application
    Filed: November 25, 2009
    Publication date: September 2, 2010
    Applicant: LIFE TECHNOLOGIES CORPORATION
    Inventors: Mark T. Reed, Albert L. Carrillo, Ian A. Harding
  • Publication number: 20100196212
    Abstract: A filling apparatus for filling a microplate. The microplate can comprise a plurality of wells each sized to receive an assay. A substrate can comprise a first surface and an opposing second surface, a first assay input port for receiving the assay disposed on the first surface, a plurality of staging capillaries extending through the substrate, and a first plurality of microfluidic channels fluidly coupling the first assay input port with at least one of the plurality of staging capillaries. Each of the plurality of staging capillaries can comprise an inlet and an outlet and be sized to receive the assay.
    Type: Application
    Filed: November 30, 2009
    Publication date: August 5, 2010
    Applicant: LIFE TECHNOLOGIES CORPORATION
    Inventors: Mark T. Reed, Albert L. Carrillo, Ian A. Harding
  • Patent number: 7695688
    Abstract: A filling apparatus for filling a microplate. The microplate can comprise a plurality of wells each sized to receive an assay. A substrate can comprise a first surface and an opposing second surface, a first assay input port for receiving the assay disposed on the first surface, a plurality of staging capillaries extending through the substrate, and a first plurality of microfluidic channels fluidly coupling the first assay input port with at least one of the plurality of staging capillaries. Each of the plurality of staging capillaries can comprise an inlet and an outlet and be sized to receive the assay.
    Type: Grant
    Filed: March 22, 2005
    Date of Patent: April 13, 2010
    Assignee: Applied Biosystems, LLC
    Inventors: Mark T. Reed, Albert L. Carrillo, Ian A. Harding
  • Patent number: 7670834
    Abstract: The present application relates to an apparatus and method for thermal cycling using a source of cooling gas.
    Type: Grant
    Filed: June 23, 2005
    Date of Patent: March 2, 2010
    Assignee: Applied Biosystems, LLC
    Inventors: Adrian Fawcett, Mark T. Reed
  • Patent number: 7604937
    Abstract: The present invention is directed to methods, reagents, and kits for detecting the presence or absence of (or quantifying) target polynucleotide sequences in at least one sample using encoding and decoding reactions. When a particular target polynucleotide is present in a sample, a reaction product is formed in the encoding reaction that includes addressable primer portions. At least one label and at least one address primer is employed in the decoding amplification reaction thereby providing a detectable signal value depending upon whether a sequence is present or absent. In some embodiments universal bases and reduced libraries of probes can be employed for highly multiplexed analysis of target polynucleotides.
    Type: Grant
    Filed: March 24, 2005
    Date of Patent: October 20, 2009
    Assignee: Applied Biosystems, LLC
    Inventors: Kai Qin Lao, Mark T. Reed
  • Publication number: 20090197277
    Abstract: A filling apparatus for filling a microplate. The microplate having a plurality of wells each sized to receive an assay. The filling apparatus can comprise an assay input layer having a first surface and an opposing second surface. The assay input layer can comprise an assay input port extending from the first surface to the second surface and at least one pressure nodule extending from the second surface. An output layer can comprise a plurality of staging capillaries each having an inlet and an outlet. A intermediate layer can be disposed between the assay input layer and the output layer. The intermediate layer can be flexible and comprise a plurality of microfluidic channels and a flow aperture being in fluid communication with the assay input port and the plurality of microfluidic channels.
    Type: Application
    Filed: April 2, 2009
    Publication date: August 6, 2009
    Applicant: Life Technologies Corporation
    Inventors: Nigel P. Beard, Albert L. Carrillo, Ian A. Harding, Mark T. Reed
  • Publication number: 20090191098
    Abstract: A filling apparatus for filling a microplate. The microplate having a plurality of wells each sized to receive an assay. The filling apparatus can comprise an assay input layer having a first surface and an opposing second surface. The assay input layer can comprise an assay input port extending from the first surface to the second surface and at least one pressure nodule extending from the second surface. An output layer can comprise a plurality of staging capillaries each having an inlet and an outlet. The output layer can further comprise a capillary plane disposed above the plurality of staging capillaries in fluid communication with the assay input port. The capillary plane can be sized to draw the assay from the assay input port to generally flood fill the plurality of staging capillaries.
    Type: Application
    Filed: April 6, 2009
    Publication date: July 30, 2009
    Applicant: Life Technologies Corporation
    Inventors: Nigel P. Beard, Mark T. Reed
  • Publication number: 20080274511
    Abstract: The invention relates to a device for carrying out of chemical or biological reactions with a reaction vessel receiving element for receiving a microtiter plate with several reaction vessels, wherein the reaction vessel receiving element has several recesses arranged in a regular pattern to receive the respective reaction vessels, a heating device for heating the reaction vessel receiving element, and a cooling device for cooling the reaction vessel. The invention is characterized by the fact that the reaction vessel receiving element is divided into several segments. The individual segments are thermally decoupled from one another, and each segment is assigned a heating device which may be actuated independently of the others. By means of the segmentation of the reaction vessel receiving element, it is possible for zones to be set and held at different temperatures.
    Type: Application
    Filed: September 5, 2007
    Publication date: November 6, 2008
    Inventors: Lim Hi TAN, Jew Kween Ngui, Hon Siu Shin, Ui Leng Soh, Yang Hooi Kee, Hock Lai Khoo, Mark T. Reed, Ching Ong Lau, Wolfgang Heimberg
  • Patent number: 7407630
    Abstract: A filling apparatus for filling a microplate. The microplate having a plurality of wells each sized to receive an assay. The filling apparatus can comprise an assay input layer having a first surface and an opposing second surface. The assay input layer can comprise an assay input port extending from the first surface to the second surface, a venting manifold formed in the second surface, and a vent hole in fluid communication with the venting manifold and atmosphere. An output layer can comprise a plurality of staging capillaries each having an inlet and an outlet. A venting layer can be disposed between the assay input layer and the output layer. The venting layer can comprise a plurality of microfluidic channels, a flow aperture being in fluid communication with the assay input port and the plurality of microfluidic channels, and a plurality of vent apertures each in fluid communication with at least one of the plurality of staging capillaries.
    Type: Grant
    Filed: March 22, 2005
    Date of Patent: August 5, 2008
    Assignee: Applera Corporation
    Inventors: Mark T. Reed, Albert L. Carrillo, Ian A. Harding
  • Patent number: 7323705
    Abstract: The present application relates to an apparatus and method for measuring liquid levels in small-volume wells.
    Type: Grant
    Filed: May 16, 2005
    Date of Patent: January 29, 2008
    Assignee: Applera Corporation
    Inventors: Douwe D. Haga, Michael R. Flatbush, Michael Mosseau, Tony S. Yan, Willy Wiyatno, Mark T. Reed
  • Patent number: 6896849
    Abstract: A multi-well microfiltration apparatus and method are provided and feature a manual touch-off system for transferring pendent drops hanging from discharge-conduits of a discharge-conduit array to respective receiving wells or receiving holes of a corresponding receiving array, with minimum or no cross-contamination between the discharge conduits, or the receiving wells or receiving holes. The manual touch-off is achieved by manually shifting a carriage that supports one of the arrays, into a position whereat pendent drops of fluid hanging from the distal ends of the discharge conduits contact the inner sidewalls of the corresponding receiving wells or receiving holes of the receiving array.
    Type: Grant
    Filed: March 22, 2002
    Date of Patent: May 24, 2005
    Assignee: Applera Corporation
    Inventors: Mark T. Reed, Jon Hoshizaki
  • Publication number: 20030215956
    Abstract: The present invention provides multi-well plates and column arrays in which samples (e.g., cell lysates containing nucleic acids of interest, such as RNA) can be analyzed and/or processed. In one embodiment, the microfiltration arrangement is a multilayer structure, including (i) a column plate having an array of minicolumns into which samples can be placed, (ii) a discrete filter element disposed in each minicolumn, (iii) a drip-director plate having a corresponding array of drip directors through which filtrate may egress, and (iv) a receiving-well plate having a corresponding array of receiving wells into which filtrate can flow. The invention provides multi-well microfiltration arrangements that are relatively simple to manufacture and that overcome many of the problems associated with the prior arrangements relating to (i) cross-contamination due to wicking across a common filter sheet or (ii) individual filter elements entrapping sample constituents within substantial dead volumes.
    Type: Application
    Filed: June 12, 2003
    Publication date: November 20, 2003
    Inventor: Mark T. Reed
  • Publication number: 20020150505
    Abstract: A multi-well microfiltration apparatus and method are provided and feature a manual touch-off system for transferring pendent drops hanging from discharge-conduits of a discharge-conduit array to respective receiving wells or receiving holes of a corresponding receiving array, with minimum or no cross-contamination between the discharge conduits, or the receiving wells or receiving holes. The manual touch-off is achieved by manually shifting a carriage that supports one of the arrays, into a position whereat pendent drops of fluid hanging from the distal ends of the discharge conduits contact the inner sidewalls of the corresponding receiving wells or receiving holes of the receiving array.
    Type: Application
    Filed: March 22, 2002
    Publication date: October 17, 2002
    Inventors: Mark T. Reed, Jon Hoshizaki