Patents by Inventor Marlene M. King

Marlene M. King has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Patent number: 5674717
    Abstract: Nucleic acids can be amplified and detected using a very rapid polymerase chain reaction procedure in which two different nucleic acid sequences are present. This method allows one to preferentially modulate (for example, suppress) the degree of amplification of one or more nucleic acid sequences relative to other nucleic acid sequences. This modulation is achieved by exploiting differences in the relative primer melt temperatures, or by using certain ratios of primers. Each PCR cycle is very fast, that is less than about 90 seconds. This method is particularly useful for amplification and detection of DNA associated with infectious agents that may be present in a specimen in very small quantities compared to other nontargeted nucleic acids.
    Type: Grant
    Filed: October 25, 1995
    Date of Patent: October 7, 1997
    Assignee: Johnson & Johnson Clinical Diagnostics, Inc.
    Inventors: John W. Backus, William Harold Donish, John Bruce Findlay, John William H. Sutherland, Marlene M. King
  • Patent number: 5514550
    Abstract: A nucleic acid test article can be used to detect a targeted nucleic acid found in a specimen. The test article includes a substrate having two opposing surfaces and a water-insoluble nucleic acid probe attached in a distinct zone of one of the surfaces. The probe is prepared from a water-insoluble particle to which is covalently attached an oligonucleotide which is complementary to the targeted nucleic acid. Substantially none of the probe is embedded within the surface of the substrate. Particularly useful test articles have a multiplicity of water-insoluble probes located in distinct zones on one of the substrate surfaces. These probes are useful for the detection of a multiplicity of targeted nucleic acids, particularly after amplification by polymerase chain reaction.
    Type: Grant
    Filed: September 2, 1994
    Date of Patent: May 7, 1996
    Assignee: Johnson & Johnson Clinical Diagnostics, Inc.
    Inventors: John B. Findlay, Janice M. Mayer, Marlene M. King, Fred T. Oakes, Chu-an Chang, Corey H. Levenson
  • Patent number: 5328825
    Abstract: An oligonucleotide is linked to a particle through a protein or carbohydrate to form a water-insoluble nucleic acid probe. The protein or carbohydrate has a pI of about 6 or less, and has been chemically modified with an acylating, alkylating or sulfonylating agent. The particle surface is substantially free of other proteins or carbohydrates. The probe is useful in various diagnostic and purification methods wherein hybridization of the oligonucleotide with a target nucleic acid is possible. In one instance, the probe can be used to capture a DNA strand which has been amplified using polymerase chain reaction techniques.
    Type: Grant
    Filed: March 6, 1992
    Date of Patent: July 12, 1994
    Assignee: Eastman Kodak Company
    Inventors: Harold C. Warren, III, John B. Findlay, Marlene M. King