Patents by Inventor Marlene VUILLEMIN
Marlene VUILLEMIN has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Patent number: 11345906Abstract: The present invention relates to novel proteases, more particularly to protease variants having improved activity compared to the protease of SEQ ID N° 1 and the uses thereof for degrading polyester containing material, such as plastic products. The proteases of the invention are particularly suited to degrade polylactic acid, and material containing polylactic acid.Type: GrantFiled: September 30, 2020Date of Patent: May 31, 2022Assignee: CARBIOSInventors: Alain Marty, Sophie Duquesne, Marie Guicherd, Marlene Vuillemin, Maher Ben Khaled
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Patent number: 11253537Abstract: Polypeptides having the ability to specifically form connections of glucosyl units in alpha 1,3 on an acceptor having at least one hydroxyl moiety are presented. The polypeptides include i) the pattern I of sequence SEQ ID NO: 1, ii) the pattern II of sequence SEQ ID NO: 2, iii) the pattern III of sequence SEQ ID NO: 3, and iv) the pattern IV of sequence SEQ ID NO: 4, or derivates from one or several of said patterns, wherein the polypeptide furthermore has an aspartic residue (D) at position 5 of the pattern II (SEQ ID NO: 2), a glutamic acid residue (E) at position 6 of the pattern III (SEQ ID NO: 3) an an aspartic acid residue (D) at position 6 of the pattern IV (SEQ ID NO: 4). Methods for producing acceptors connected to glucosyl units in alpha 1,3 using the polypeptides are also provided.Type: GrantFiled: June 29, 2020Date of Patent: February 22, 2022Assignees: INSTITUT NATIONAL DE RECHERCHE POUR L'AGRICULTURE, L'ALIMENTATION ET L'ENVIRONNEMENT, INSTITUT NATIONAL DES SCIENCES APPLIQUEES DE TOULOUSE, CENTRE NATIONAL DE LA RECHERCHE SCIENTIFIQUE (CNRS)Inventors: Magali Remaud-Simeon, Marlene Vuillemin, Claire Moulis, Pierre Monsan, Sandrine Morel
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Publication number: 20210009980Abstract: The present invention relates to novel proteases, more particularly to protease variants having improved activity compared to the protease of SEQ ID N° 1 and the uses thereof for degrading polyester containing material, such as plastic products. The proteases of the invention are particularly suited to degrade polylactic acid, and material containing polylactic acid.Type: ApplicationFiled: September 30, 2020Publication date: January 14, 2021Inventors: ALAIN MARTY, SOPHIE DUQUESNE, MARIE GUICHERD, MARLENE VUILLEMIN, MAHER BEN KHALED
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Patent number: 10842808Abstract: Polypeptides having the ability to specifically form connections of glucosyl units in alpha 1,3 on an acceptor having at least one hydroxyl moiety are presented. The polypeptides include i) the pattern I of sequence SEQ ID NO: 1, ii) the pattern II of sequence SEQ ID NO: 2, iii) the pattern III of sequence SEQ ID NO: 3, and iv) the pattern IV of sequence SEQ ID NO: 4, or derivates from one or several of said patterns, wherein the polypeptide furthermore has an aspartic residue (D) at position 5 of the pattern II (SEQ ID NO: 2), a glutamic acid residue (E) at position 6 of the pattern III (SEQ ID NO: 3) and an aspartic acid residue (D) at position 6 of the pattern IV (SEQ ID NO: 4). Methods for producing acceptors connected to glucosyl units in alpha 1,3 using the polypeptides are also provided.Type: GrantFiled: September 22, 2017Date of Patent: November 24, 2020Assignees: INSTITUT NATIONAL DE RECHERCHE POUR L'AGRICULTURE, L'ALIMENTATION ET L'ENVIRONNEMENT, INSTITUT NATIONAL DES SCIENCES APPLIQUEES DE TOULOUSE, CENTRE NATIONAL DE LA RECHERCHE SCIENTIFIQUE (CNRS)Inventors: Magali Remaud-Simeon, Marlene Vuillemin, Claire Moulis, Pierre Monsan, Sandrine Morel
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Patent number: 10829754Abstract: The present invention relates to novel proteases, more particularly to protease variants having improved activity compared to the protease of SEQ ID NO:1 and the uses thereof for degrading polyester containing material, such as plastic products. The proteases of the invention are particularly suited to degrade polylactic acid, and material containing polylactic acid.Type: GrantFiled: December 15, 2017Date of Patent: November 10, 2020Assignee: CARBIOSInventors: Alain Marty, Sophie Duquesne, Marie Guicherd, Marlene Vuillemin, Maher Ben Khaled
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Publication number: 20200323899Abstract: Polypeptides having the ability to specifically form connections of glucosyl units in alpha 1,3 on an acceptor having at least one hydroxyl moiety are presented. The polypeptides include i) the pattern I of sequence SEQ ID NO: 1, ii) the pattern II of sequence SEQ ID NO: 2, iii) the pattern III of sequence SEQ ID NO: 3, and iv) the pattern IV of sequence SEQ ID NO: 4, or derivates from one or several of said patterns, wherein the polypeptide furthermore has an aspartic residue (D) at position 5 of the pattern II (SEQ ID NO: 2), a glutamic acid residue (E) at position 6 of the pattern III (SEQ ID NO: 3) an an aspartic acid residue (D) at position 6 of the pattern IV (SEQ ID NO: 4). Methods for producing acceptors connected to glucosyl units in alpha 1,3 using the polypeptides are also provided.Type: ApplicationFiled: June 29, 2020Publication date: October 15, 2020Inventors: Magali REMAUD-SIMEON, Marlene VUILLEMIN, Claire MOULIS, Pierre MONSAN, Sandrine MOREL
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Patent number: 10696995Abstract: The present invention relates to a method for producing a glycosylated synthon or a monomer. Said method includes at least one step of placing at least one glycan-saccharase in the presence of at least one hydroxylated synthon and at least one saccharose. The invention also relates to a method for producing a glyco(co)polymer, including polymerizing at least two monomers separately obtained from the enzymatic glycosylation method according to the invention, and to a method for producing a glyco(co)polymer, preferably a block glyco(co)polymer, including coupling at least two monomers separately obtained from the enzymatic glycosylation method according to the invention.Type: GrantFiled: March 17, 2016Date of Patent: June 30, 2020Assignees: INSTITUT NATIONAL DE RECHERCHE POUR L'AGRICULTURE, L'ALIMENTATION ET L'ENVIRONNEMENT, INSTITUT NATIONAL DES SCIENCES APPIQUEES DE TOULOUSE (INSAT), CENTRE NATIONAL DE LA RECHERCHE SCIENTIFIQUE, UNIVERSITE DE BORDEAUX, INSTITUT POLYTECHNIQUE DE BORDEAUXInventors: Isabelle Andre, Stephane Grelier, David Guieysse, Alvaro Lafraya, Pierre Monsan, Claire Moulis, Frederic Peruch, Magali Remaud-Simeon, Marlene Vuillemin
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Publication number: 20190345472Abstract: The present invention relates to novel proteases, more particularly to protease variants having improved activity compared to the protease of SEQ ID NO:1 and the uses thereof for degrading polyester containing material, such as plastic products. The proteases of the invention are particularly suited to degrade polylactic acid, and material containing polylactic acid.Type: ApplicationFiled: December 15, 2017Publication date: November 14, 2019Inventors: ALAIN MARTY, SOPHIE DUQUESNE, MARIE GUICHERD, MARLENE VUILLEMIN, MAHER BEN KHALED
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Patent number: 10316300Abstract: The invention relates to: —a protein having dextran-saccharase activity and the sequence SEQ ID NO: 1 for an amino acid sequence; —a protein having dextran-saccharase activity and the sequence SEQ ID NO: 2 for an amino acid sequence; —a complex containing a substrate and a protein with dextran-saccharase activity —a method for synthesizing dextrans; and —dextrans. The invention also relates to a method for synthesizing gluco-oligosaccharides and gluco-conjugates and to the resulting products.Type: GrantFiled: July 21, 2015Date of Patent: June 11, 2019Assignees: INSTITUT NATIONAL DES SCIENCES APPLIQUEES TOULOUSE, INSTITUT NATIONAL DE LA RECHERCHE AGRONOMIQUE, CENTRE NATIONAL DE LA RECHERCHE SCIENTIFIQUE, UNIVERSITE PAUL SABATIER TOULOUSE IIIInventors: Marlène Vuillemin, Marion Claverie, Claire Moulis, Magali Remaud-Simeon, Pierre Monsan, Etienne Severac, Catherine Fontagne-Faucher
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Patent number: 10308724Abstract: The subject matter of the invention is dextrans which have between 95% and 99% of ?-1,6 glucosidic bonds, a weight-average molar mass Mw at least equal to 0.7×109 g.mol?1, and a dispersity index D of between 1.3 and 3. The invention also relates to a dextran saccharase which makes it possible to produce such dextrans, and to a method for producing said dextrans.Type: GrantFiled: June 19, 2015Date of Patent: June 4, 2019Assignees: INSTITUT NATIONAL DES SCIENCES APPLIQUEES TOULOUSE, INSTITUT NATIONAL DE LA RECHERCHE AGRONOMIQUE, CENTRE NATIONAL DE LA RECHERCHE SCIENTIFIQUE, UNIVERSITE DE BORDEAUX, INSTITUT POLYTECHNIQUE DE BORDEAUXInventors: Marlène Vuillemin, Marion Claverie, Claire Moulis, Magali Remaud-Simeon, Florent Grimaud, Pierre Monsan, Agnès Sabate, Catherine Garnier, Marguerite Dols-Lafargue, Patrick Lucas
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Publication number: 20180258456Abstract: The present invention relates to a method for producing a glycosylated synthon or a monomer. Said method includes at least one step of placing at least one glycan-saccharase in the presence of at least one hydroxylated synthon and at least one saccharose. The invention also relates to a method for producing a glyco(co)polymer, including polymerizing at least two monomers separately obtained from the enzymatic glycosylation method according to the invention, and to a method for producing a glyco(co)polymer, preferably a block glyco(co)polymer, including coupling at least two monomers separately obtained from the enzymatic glycosylation method according to the invention.Type: ApplicationFiled: March 17, 2016Publication date: September 13, 2018Inventors: Isabelle Andre, Stephane Grelier, David Guieysse, Alvaro Lafraya, Pierre Monsan, Claire Moulis, Frederic Peruch, Magali Remaud-Simeon, Marlene Vuillemin
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Publication number: 20180104274Abstract: Polypeptides having the ability to specifically form connections of glucosyl units in alpha 1,3 on an acceptor having at least one hydroxyl moiety are presented. The polypeptides include i) the pattern I of sequence SEQ ID NO: 1, ii) the pattern II of sequence SEQ ID NO: 2, iii) the pattern III of sequence SEQ ID NO: 3, and iv) the pattern IV of sequence SEQ ID NO: 4, or derivates from one or several of said patterns, wherein the polypeptide furthermore has an aspartic residue (D) at position 5 of the pattern II (SEQ ID NO: 2), a glutamic acid residue (E) at position 6 of the pattern III (SEQ ID NO: 3) and an aspartic acid residue (D) at position 6 of the pattern IV (SEQ ID NO: 4). Methods for producing acceptors connected to glucosyl units in alpha 1,3 using the polypeptides are also provided.Type: ApplicationFiled: September 22, 2017Publication date: April 19, 2018Inventors: Magali REMAUD-SIMEON, Marlene VUILLEMIN, Claire MOULIS, Pierre MONSAN, Sandrine MOREL
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Publication number: 20170247669Abstract: The invention relates to: a protein having dextran-saccharase activity and the sequence SEQ ID NO: 1 for an no acid sequence; a protein having dextran-saccharase activity and the sequence SEQ ID NO: 2 for an amino acid sequence; a complex containing a substrate and a protein with dextran-saccharase activity a method for synthesizing dextrans; and dextrans. The invention also relates to a method for synthesizing gluco-oligosaccharides and gluco-conjugates and to the resulting products.Type: ApplicationFiled: July 21, 2015Publication date: August 31, 2017Inventors: Marlène Vuillemin, Marion Claverie, Claire Moulis, Magali Remaud-Simeon, Pierre Monsan, Etienne Severac, Catherine Fontagne-Faucher
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Publication number: 20170145120Abstract: The subject matter of the invention is dextrans which have between 95% and 99% of ?-1,6 glucosidic bonds, a weight-average molar mass Mw at least equal to 0.7×109 g.mol?1, and a dispersity index D of between 1.3 and 3. The invention also relates to a dextran saccharase which makes it possible to produce such dextrans, and to a method for producing said dextrans.Type: ApplicationFiled: June 19, 2015Publication date: May 25, 2017Inventors: Marlène Vuillemin, Marion Claverie, Claire Moulis, Magali Remaud-Simeon, Florent Grimaud, Pierre Monsan, Agnès Sabate, Catherine Garnier, Marguerite Dols-Lafargue, Patrick Lucas
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Publication number: 20160136199Abstract: An isolated polypeptide having the ability to specifically form connections of glucosyl units in alpha 1,3 on an acceptor including at least one hydroxyl moiety. The polypeptide includes i) the pattern I of sequence SEQ ID NO: 1, ii) the pattern II of sequence SEQ ID NO: 2, iii) the pattern II of sequence SEQ ID NO: 3, iv) the pattern IV of sequence SEQ ID NO: 4 or derivates from one or several of said patterns. The polypeptide furthermore has the aspartic residue (D) in position 5 of the pattern II (SEQ ID NO: 2), the glutamic acid residue (E) at position 6 of the pattern III (SEQ ID NO: 3) and the aspartic acid residue (D) in position 6 of the pattern IV (SEQ ID NO: 4); and its uses.Type: ApplicationFiled: June 17, 2014Publication date: May 19, 2016Inventors: Magali REMAUD-SIMEON, Marlene VUILLEMIN, Claire MOULIS, Pierre MONSAN, Sandrine MOREL