Patents by Inventor Martin Gilar
Martin Gilar has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Patent number: 11918936Abstract: The present disclosure discusses a method of separating a sample including oligonucleotides including coating a flow path of a chromatographic system; injecting the sample comprising oligonucleotides into the chromatographic system; flowing the sample through the chromatographic system; and separating the oligonucleotides. In some examples, the coating of the flow path is non-binding with respect to the analyte, such as oligonucleotides. Consequently, the analyte does not bind to the coating of the flow path. The non-binding coating eliminates the need for passivation, which can eliminate the formerly needed time to passivate as well. In addition, analyte can be recovered with a first injection in a system, such as chromatographic system.Type: GrantFiled: January 15, 2021Date of Patent: March 5, 2024Assignee: Waters Technologies CorporationInventors: Michael Donegan, Martin Gilar, Armand Gatien Ngounou Wetie
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Patent number: 11912983Abstract: The present technology relates to a method of separating a sample comprising oligonucleotides. The method includes injecting a polyphosphonic acid at a concentration of between about 0.01 M to about 1 M into the sample comprising oligonucleotides. The method also includes flowing the sample and polyphosphonic acid through a liquid chromatography column and separating the oligonucleotides.Type: GrantFiled: December 4, 2020Date of Patent: February 27, 2024Assignee: Waters Technologies CorporationInventors: Michael Donegan, Martin Gilar, Matthew Lauber, Scott Mccall, Pamela Iraneta, Kerri Smith
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Publication number: 20240043907Abstract: The present disclosure describes methods, kits, and systems for digesting polyribonucleotides. The method involves selectively forming oligonucleotide (e.g., DNA:RNA or RNA:RNA) duplexes with single-stranded target RNA and then using sequence-specific nucleases that only act on RNA within duplexes to selectively cleave the target RNA into smaller fragments. Additional sequence-specific ribonucleases may be used to provide additional cuts of the target RNA at predetermined sites. By forming duplexes to increase the availability of nucleases that may be applied to cleave the single-stranded target RNA and selectively control where the target RNA is cleaved, the target RNA may be digested into fragments within controllable size ranges that are optimal for polynucletide analysis, such as by liquid chromatography and mass spectrometry.Type: ApplicationFiled: August 7, 2023Publication date: February 8, 2024Applicant: Waters Technologies CorporationInventors: Martin Gilar, Catalin Doneanu, Matthew A. Lauber, Mame Maissa Gaye
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Patent number: 11842891Abstract: An ion detector comprises a surface configured to receive one or more ions and a detector configured to detect one or more ions by detecting electromagnetic radiation scattered by one or more ions at the surface.Type: GrantFiled: April 8, 2021Date of Patent: December 12, 2023Assignee: Waters Technologies CorporationInventors: Joseph A. Jarrell, Martin Gilar
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Publication number: 20230364585Abstract: The present disclosure discusses a method of separating and/or purifying polynucleotides. The method includes injecting a sample into a chromatographic column that is packed with a porous sorbent having a pore size that substantially excludes the polynucleotides from the sorbent. This restricted access to the sorbent allows separation of large polynucleotides from each other and from smaller molecular weight impurities.Type: ApplicationFiled: May 8, 2023Publication date: November 16, 2023Applicant: Waters Technologies CorporationInventors: Matthew A. Lauber, Catalin Doneanu, Weibin Chen, Ying Qing Yu, Jennifer M. Nguyen, Fabrice Gritti, Martin Gilar
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Patent number: 11709085Abstract: Described is a method for determining a dwell volume of a liquid chromatography system and a liquid chromatography system that can determined the system dwell volume. The method includes mixing a flow of a first solvent with a flow of a second solvent to form a solvent mixture. The flows of the first and second solvents are decreased and increased, respectively, to generate a gradient composition. A system pressure of the liquid chromatography system is measured to determine a pressure trace defined as the measured system pressure as a function of time. The dwell volume of the system is determined from a time delay determined between the gradient composition at the mixing location and the pressure trace. The method can be performed with a liquid chromatography system having a chromatographic column or a flow restrictor used in place of the chromatographic column.Type: GrantFiled: August 19, 2020Date of Patent: July 25, 2023Assignee: Waters Technologies CorporationInventors: Jason F. Hill, Martin Gilar, Abhijit Tarafder, Christopher DesJardins
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Patent number: 11604174Abstract: Exemplary embodiments are directed to methods and systems for controlling fluid parameters within a detector. A first and second fluid pump manager control the flow of a first and second fluid to one or more heating/cooling devices, and a mixer receives and mixes the first and second fluids. An optical detector flow cell receives the fluid mixture from the mixer, and a pressure regulator controls the pressure at the optical detector flow cell. Thus, the composition, temperature, and pressure of a fluid mixture entering an optical detector flow cell can be controlled in real time.Type: GrantFiled: November 15, 2019Date of Patent: March 14, 2023Assignee: Waters Technologies CorporationInventors: Abhijit Tarafder, Sebastien Besner, Mark Basile, Martin Gilar
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Publication number: 20220017887Abstract: The present disclosure relates to compositions, kits and methods that may be used for removal of matrix components, including proteins and lipids, from one or more oligonucleotides.Type: ApplicationFiled: July 14, 2021Publication date: January 20, 2022Inventors: Michael Donegan, Martin Gilar, Darryl W. Brousmiche
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Publication number: 20210396722Abstract: Exemplary embodiments eliminate the need for column ovens in serial column chromatography arrangements and systems by using insulated sleeves. The insulated sleeves may encase individual chromatography columns or clusters of chromatography columns. The use of the insulated sleeves allows the chromatography columns to be positioned in close proximity to each other. This may decrease the overall size of a serial column chromatography arrangement and may reduce costs by not requiring the column ovens.Type: ApplicationFiled: June 22, 2021Publication date: December 23, 2021Inventors: Michael O. Fogwill, Martin Gilar, Joseph D. Michienzi, Wade P. Leveille, Geoff C. Gerhardt, Jeffrey Musacchio, Jason F. Hill, Sylvain G. Cormier
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Publication number: 20210396721Abstract: The exemplary embodiments may provide a collective insulating sleeve for a plurality of chromatography columns or may provide separate insulating sleeve for each of the chromatography columns in a plurality. As a result, column ovens are not needed, and pre-heaters may not be required for each chromatography column in some exemplary embodiments. Thus, parallel column arrangements in the exemplary embodiments may be more compact than conventional arrangements.Type: ApplicationFiled: June 22, 2021Publication date: December 23, 2021Inventors: Michael O. Fogwill, Martin Gilar, Joseph D. Michienzi, Wade P. Leveille, Geoff C. Gerhardt, Jeffrey Musacchio, Jason F. Hill, Sylvain G. Cormier
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Publication number: 20210319994Abstract: An ion detector comprises a surface configured to receive one or more ions and a detector configured to detect one or more ions by detecting electromagnetic radiation scattered by one or more ions at the surface.Type: ApplicationFiled: April 8, 2021Publication date: October 14, 2021Applicant: Waters Technologies CorporationInventors: Joseph A. Jarrell, Martin Gilar
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Patent number: 11105778Abstract: A method for injecting a diluted sample in a chromatography system includes merging a flow of a sample and a flow of a diluent to form a flow of a diluted sample. A dilution ratio of the diluted sample equals a sum of the volumetric flow rates of the sample and the diluent divided by the volumetric flow rate of the sample. The diluted sample is stored in a holding element before injection into a chromatographic system flow. Sample dilution occurs under low pressure relative to the chromatographic flow thereby allowing lower pressure sample and diluent syringes to be used. Other benefits include reduced compressibility and a reduction in leaks due to the lower pressure operation. The method avoids problems associated with manual techniques which can introduce errors due, for example, to loss of sample, sample precipitation and adsorption of sample to vials.Type: GrantFiled: May 4, 2017Date of Patent: August 31, 2021Assignee: WATERS TECHNOLOGIES CORPORATIONInventors: Martin Gilar, Richard W. Andrews, Peyton C. Beals, Thomas McDonald, Keith Fadgen, Jordan Kornfeld, Geoff Gerhardt, Sylvain Cormier
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Publication number: 20210220755Abstract: The present disclosure discusses a method of separating a sample including oligonucleotides including coating a flow path of a chromatographic system; injecting the sample comprising oligonucleotides into the chromatographic system; flowing the sample through the chromatographic system; and separating the oligonucleotides. In some examples, the coating of the flow path is non-binding with respect to the analyte, such as oligonucleotides. Consequently, the analyte does not bind to the coating of the flow path. The non-binding coating eliminates the need for passivation, which can eliminate the formerly needed time to passivate as well. In addition, analyte can be recovered with a first injection in a system, such as chromatographic system.Type: ApplicationFiled: January 15, 2021Publication date: July 22, 2021Applicant: Waters Technologies CorporationInventors: Michael Donegan, Martin Gilar, Armand Gatien Ngounou Wetie
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Patent number: 11061003Abstract: Described are a method and apparatus for diluting a chromatographic sample. The method includes repeating an alternating acquisition of sample fluidic plugs each having an incremental volume of sample and diluent fluidic plugs each having an incremental volume of diluent to obtain a stack of alternating sample and diluent fluidic plugs. The stack is inserted into a flow of a mobile phase in a chromatography system. Alternatively, the method includes repeating the steps of injecting an incremental volume of sample into a chromatographic system flow and providing an incremental volume of mobile phase into the chromatographic system flow. In either implementation, the dilution ratio of the sample equals the sum of the incremental volumes of the sample and the diluent or mobile phase divided by the sum of the incremental volumes of the sample.Type: GrantFiled: October 15, 2019Date of Patent: July 13, 2021Assignee: WATERS TECHNOLOGIES CORPORATIONInventors: Martin Gilar, Keith Fadgen, Peyton C. Beals, Sylvain Gilles Cormier, Richard Wayne Andrews, Geoff C. Gerhardt, Jordan Kornfeld, Thomas S. McDonald
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Publication number: 20210199630Abstract: Methods and devices for the washing, extraction, and separation of a sample in a disposable chromatography cartridge (201) comprising a barrel (204) and a column (205), and especially including reinforcement to the column permitting high-pressure separation.Type: ApplicationFiled: May 20, 2019Publication date: July 1, 2021Applicant: Waters Technologies CorporationInventors: Martin Gilar, Thomas S. McDonald, Joseph D. Michienzi, Jeffrey Musacchio, Rachel Mumma, Keith Fadgen, Geoff C. Gerhardt
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Publication number: 20210199624Abstract: Sample preparation and separation can be performed using a sample cartridge (201). The cartridge includes a barrel (204) with a first and second end, a column segment (209) connected to the second end of the barrel, and a column (205) containing a sorbent material. The sorbent material includes particles that have antibodies attached to them to selectively retain analytes, proteins attached to them to retain certain classes of antibodies, or enzymes attached to them to perform specific modifications to certain classes of molecules. The column segment can be in thermal communication with a temperature control device in order to control the temperature of the column.Type: ApplicationFiled: May 20, 2019Publication date: July 1, 2021Applicant: Waters Technologies CorporationInventors: Martin Gilar, Thomas S. McDonald, Joseph D. Michienzi, Jeffrey Musacchio, Rachel Mumma, Keith Fadgen, Geoff C. Gerhardt
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Publication number: 20210171932Abstract: The present technology relates to a method of separating a sample comprising oligonucleotides. The method includes injecting a polyphosphonic acid at a concentration of between about 0.01 M to about 1 M into the sample comprising oligonucleotides. The method also includes flowing the sample and polyphosphonic acid through a liquid chromatography column and separating the oligonucleotides.Type: ApplicationFiled: December 4, 2020Publication date: June 10, 2021Applicant: Waters Technologies CorporationInventors: Michael Donegan, Martin Gilar, Matthew Lauber, Scott Mccall, Pamela Iraneta, Kerri Smith
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Publication number: 20210156826Abstract: Described herein are a selectable flow restriction device and a fluidic network for chromatographic sample dilution during injection. A selectable flow restriction device is used in combination with a splitter to define an adjustable splitter for separating a high-pressure solvent flow into two separate solvent flows having the same solvent composition. The first solvent flow passes to a sample manager where a sample is injected as a discrete fluidic plug at an injection valve into a flow comprising at least one solvent. The second solvent flow is merged downstream from the location of injection with the first solvent carrying the sample fluidic plug. The second solvent flow dilutes the sample plug during the mixture of the two solvent flows.Type: ApplicationFiled: November 11, 2020Publication date: May 27, 2021Inventors: Jason F. Hill, Sylvain Gilles Cormier, Martin Gilar
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Publication number: 20210055149Abstract: Described is a method for determining a dwell volume of a liquid chromatography system and a liquid chromatography system that can determined the system dwell volume. The method includes mixing a flow of a first solvent with a flow of a second solvent to form a solvent mixture. The flows of the first and second solvents are decreased and increased, respectively, to generate a gradient composition. A system pressure of the liquid chromatography system is measured to determine a pressure trace defined as the measured system pressure as a function of time. The dwell volume of the system is determined from a time delay determined between the gradient composition at the mixing location and the pressure trace. The method can be performed with a liquid chromatography system having a chromatographic column or a flow restrictor used in place of the chromatographic column.Type: ApplicationFiled: August 19, 2020Publication date: February 25, 2021Inventors: Jason F. Hill, Martin Gilar, Abhijit Tarafder, Christopher DesJardins
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Publication number: 20200271629Abstract: The present disclosure relates to the determination of analytes in a sample using chromatography. The present disclosure provides methods of separating an analyte from a sample. The method includes introducing a sample comprising the analytes into a chromatographic system. The chromatographic system has a flow path disposed in an interior of the chromatographic system, at least a portion of the flow path having an active coating, and a chromatographic column having a stationary phase material in an interior of the chromatographic column that facilitates separation of the analytes in the sample through interaction with at least one analyte in the sample. The active coating is selected to interact with at least one analyte in the sample through (1) a repulsive force, (2) a secondary interaction, or (3) a retention mechanism distinct from the interaction with the stationary phase material.Type: ApplicationFiled: February 25, 2020Publication date: August 27, 2020Applicant: Waters Technologies CorporationInventors: Matthew A. Lauber, Martin Gilar, Mathew H. DeLano, Michael Donegan