Abstract: The hammerhead ribozyme according to the present invention produces a hammerhead ribozyme—target RNA construct by base-pairing with an edited target RNA, and an editing recognition site of the hammerhead ribozyme cleaves the modification site by forming a base pair with the target RNA modification site. The cleavage of the modification site is expected to be applicable to the research and development of new drugs that can be used to prevent or treat diseases caused by the edited target RNA.

Abstract: The hammerhead ribozyme according to the present invention produces a hammerhead ribozyme—target RNA construct by base-pairing with an edited target RNA, and an editing recognition site of the hammerhead ribozyme cleaves the modification site by forming a base pair with the target RNA modification site. The cleavage of the modification site is expected to be applicable to the research and development of new drugs that can be used to prevent or treat diseases caused by the edited target RNA.

Abstract: Disclosed is a method for introducing a mutation into a gene, which comprises the following steps: a homologous recombination step of carrying out the homologous recombination between a target gene into which the mutation is to be introduced and a target recombinant vector, thereby substituting an exon in the target gene into which the mutation is to be introduced by a target DNA sequence in the target recombinant vector; and a mutation introduction step of carrying out the specific recombination between the target DNA sequence in the resulting target recombinant gene and a mutation introduction cassette of a mutation introduction vector carrying a mutated DNA sequence containing a mutant exon by the intervening action of Cre recombinase to substitute the target DNA sequence by the mutated DNA in the mutation introduction cassette, thereby producing a mutation-introduced gene into which the mutant DNA sequence has been introduced.