Patents by Inventor Masaru Honjo

Masaru Honjo has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Publication number: 20200149079
    Abstract: Provided are a recombinant microorganism comprising: a gene encoding a pyridoxine oxidase; and a gene encoding a pyridoxamine synthetase having an enzymatic activity of synthesizing pyridoxamine from pyridoxal, in which each of the gene encoding a pyridoxine oxidase and the gene encoding a pyridoxamine synthetase is introduced from outside of a bacterial cell, or is endogenous to the bacterial cell and has an enhanced expression, and a method of producing pyridoxamine or a salt thereof from pyridoxine or a salt thereof using the recombinant microorganism.
    Type: Application
    Filed: May 11, 2018
    Publication date: May 14, 2020
    Applicants: Mitsui Chemicals, Inc., RENASCIENCE CO., LTD
    Inventors: Toshihiro TATENO, Tomonori HIDESAKI, Tadashi ARAKI, Atsunori SHINDO, Yoshiko MATSUMOTO, Toshio MIYATA, Masaru HONJO
  • Publication number: 20200123581
    Abstract: A recombinant microorganism includes a gene encoding a pyridoxine dehydrogenase, a gene encoding a pyridoxamine synthetase having an enzymatic activity of synthesizing pyridoxamine from pyridoxal, and a gene encoding an amino acid regeneration enzyme having an enzymatic activity of regenerating an amino acid consumed by the pyridoxamine synthetase, in which at least two of the gene encoding the pyridoxine dehydrogenase, the gene encoding the pyridoxamine synthetase, or the gene encoding the amino acid regeneration enzyme, are introduced from outside of a bacterial cell, or are endogenous to the bacterial cell and have an enhanced expression. In addition, a recombinant microorganism into which a gene encoding a pyridoxine dehydrogenase is introduced is provided.
    Type: Application
    Filed: May 11, 2018
    Publication date: April 23, 2020
    Applicants: Mitsui Chemicals, Inc., RENASCIENCE CO., LTD
    Inventors: Toshihiro TATENO, Motoi YOKOTA, Tomonori HIDESAKI, Tadashi ARAKI, Atsunori SHINDO, Yoshiko MATSUMOTO, Toshio MIYATA, Masaru HONJO
  • Patent number: 6436674
    Abstract: A DNA encoding 20K hGH is connected directly to a gene encoding Escherichia coli OppA protein secretion signal, or a modified form thereof, and a DNA encoding signal peptidase 1 to construct a recombinant plasmid, E. coli is transformed by the plasmid and cells of the resulting E. coli transformant strain are cultured for secretory production of the 20K hGH in the E. coli periplasm. This method enables efficient secretory production of 20K hGH and easy isolation and purification of 20K hGH from the periplasm fraction because the level of impure proteins in the E. coli periplasm is low.
    Type: Grant
    Filed: October 31, 2000
    Date of Patent: August 20, 2002
    Assignee: Mitsui Chemicals, Inc.
    Inventors: Masaru Honjo, Naokazu Naitoh, Hiroshi Uchida, Daisuke Mochizuki, Kazuya Matsumoto
  • Patent number: 6399565
    Abstract: Administration of an administrative amount of an authentic human growth hormone having a molecular weight of 20,000 daltons to human adults can improve body composition, stimulate lipolysis, increase serum insulin-like growth factor I or be used in replacement therapy for human growth hormone-deficient patients.
    Type: Grant
    Filed: December 15, 1997
    Date of Patent: June 4, 2002
    Assignee: Schering Aktiengesellschaft
    Inventors: Noriaki Asada, Miwa Ikeda, Masaru Honjo, Kazutoshi Horikomi, Takeshi Kamioka
  • Patent number: 6160089
    Abstract: A DNA encoding 20K hGH is connected directly to a gene encoding Escherichia coli OppA protein secretion signal, or a modified form thereof, and a DNA encoding signal peptidase 1 to construct a recombinant plasmid, E. coli is transformed by said plasmid and cells of the resulting E. coli transformant strain are cultured for secretory production of the 20K hGH in the E. coli periplasm. This method enables efficient secretory production of 20K hGH and easy isolation and purification of 20K hGH from the periplasm fraction because the level of impure proteins in the E. coli periplasm is low.
    Type: Grant
    Filed: July 7, 1999
    Date of Patent: December 12, 2000
    Assignee: Mitsui Chemicals, Inc.
    Inventors: Masaru Honjo, Naokazu Naitoh, Hiroshi Uchida, Daisuke Mochizuki, Kazuya Matsumoto
  • Patent number: 5824502
    Abstract: Efficient secretory production of a recombinant protein in the periplasm can be attained by artificially regulating co-expression of a gene coding for the target recombinant protein and a gene coding for Sec protein involved in protein transport in E. coli cells under conditions in which excessive expression of the Sec protein is suppressed.
    Type: Grant
    Filed: March 27, 1997
    Date of Patent: October 20, 1998
    Assignee: Mitsui Chemicals, Inc.
    Inventors: Masaru Honjo, Naokazu Naito, Hiroshi Uchida
  • Patent number: 5759810
    Abstract: In producing a recombinant protein in the periplasm of Escherichia coli, the productivity of the target ecombinant protein can be promoted by coexpressing glutathione reductase artificially with the target recombinant protein.
    Type: Grant
    Filed: March 28, 1996
    Date of Patent: June 2, 1998
    Assignee: Mitsui Toatsu Chemicals, Inc.
    Inventors: Masaru Honjo, Naokazu Naito, Hiroshi Uchida
  • Patent number: 5612192
    Abstract: A neutral protease gene of Bacillus amyloliquefaciens is cloned, which gene comprises the promoter region, the ribosome binding region, the region involved in the secretion of the neutral protease, the region consisting of the structural gene for the neutral protease, and the terminator region. Each of the regions is useful as a material for construction of a recombinant DNA used for the production of proteins by culturing a host bacterium transformed with the recombinant DNA. For example, the extracellular production of neutral protease in a large amount can be accomplished by culturing B. subtilis transformed with a recombinant DNA comprising pUB110 and the neutral protease gene.
    Type: Grant
    Filed: December 20, 1994
    Date of Patent: March 18, 1997
    Assignees: Agency of Industrial Science and Technology, Extra-Ministerial Bureau of Ministry of International Trade and Industry
    Inventors: Yoshio Furutani, Hiroaki Shimada, Izumi Mita, Kazuaki Manabe, Masaru Honjo, Noboru Tomioka
  • Patent number: 5496713
    Abstract: A human growth hormone having a molecular weight of 20,000 daltons can be effectively secreted and produced in the periplasm of Escherichia coli by constructing a human growth hormone secretion plasmid comprising a vector DNA replicable in E. coli and a DNA fragment including a promoter, a ribosome binding site, a secretion signal coding region, which are all those of the neutral protease gene of B. amyloliquefaciens and a gene encoding 20 kD hGH placed just downstream from the secretion signal coding region; introducing the said plasmid into E. coli; and culturing the resultant transformed cells.
    Type: Grant
    Filed: September 9, 1993
    Date of Patent: March 5, 1996
    Assignee: Mitsui Toatsu Chemicals, Inc.
    Inventors: Masaru Honjo, Setsuo Yoshino, Akira Nakayama, Naokazu Naito
  • Patent number: 5166318
    Abstract: Modified HV1-type hirudinin which valine at the N-terminal end of the HV1-type hirudin and aspartic acid at the 5th residue of the N-terminal end were replaced with alanine and glutamic acid, respectively. A secretion plasmid into which a DNA sequence coding for a precursor with an addition of a secretion signal of neutral protease of Bacillus amyloliquefaciens at the N-terminal end of this modified HV1-type hirudin is integrated is introduced into bacteria of the genus Bacillus and the precursor is expressed intracellularly. The modified HV1-type hirudin can be efficiently secreted extracellularly while maintaining its high thrombin inhibiting activity.
    Type: Grant
    Filed: June 4, 1990
    Date of Patent: November 24, 1992
    Assignee: Mitsui Toatsu Chemicals, Incorporated
    Inventors: Yoshio Furutani, Masaru Honjo, Akira Nakayama, Koichi Kawamura, Kazunori Ando, Michiko Hori, Keiko Fukazawa
  • Patent number: 5084383
    Abstract: Residual extracellular protease activities of a Bacillus subtilis strain can be further reduced by introducing a gene for the stimulation of extracellular protease levels into the genomic DNA of the strain.The resultant strain whose extracellular protease activities are further reduced is transformed with a recombinant plasmid for secretion of a desired protein and the desired protein can be efficiently produced and accumulated in the culture medium in a large amount. The accumulated desired protein can be easily recovered from the culture medium.For example, the accumulation level of human growth hormone secreted by a B. subtilis MT-430 strain carrying recombinant DNA phGH427 for the expression and secretion of human growth hormone can be increased five to tenfold as a result of the above treatment for reduction of the residual extracellular protease activities of the extracellular protease-activity-deficient strain.
    Type: Grant
    Filed: July 18, 1990
    Date of Patent: January 28, 1992
    Assignees: Agency of Industrial Science and Technology, Ministry of International Trade and Industry
    Inventors: Yoshio Furutani, Masaru Honjo, Akira Nakayama, Koichi Kawamura, Hiroaki Shimada, Izumi Mita, Akiko Akaoka
  • Patent number: 5015574
    Abstract: A expression-secretion vector is constructed by using a DNA sequence comprising a DNA fragment obtained by subjecting a DNA sequence consisting of a region involved in the expression of a gene coding for an extracellular enzyme of a bacterium of the genus Bacillus and a region involved in the secretion of the enzyme thus expressed to a deletion in the region involved in the secretion which can enhance the extracellular production of a protein dependent on the DNA fragment. By transforming a host bacterium with a recombinant DNA molecule formed by inserting a DNA fragment comprising a gene coding for a desired protein into the expression-secretion vector and then culturing the transformed host, the extracellular production of the desired protein in a large amount can be accomplished.
    Type: Grant
    Filed: November 20, 1986
    Date of Patent: May 14, 1991
    Assignees: Agency of Industrial Science and Technology, Extra-Ministerial Bureau of Ministry of International Trade and Industry
    Inventors: Yoshio Furutani, Akira Nakayama, Masaru Honjo, Hiroaki Shimada, Kouichi Kawamura, Izumi Mita, Akiko Akaoka
  • Patent number: 4824782
    Abstract: This invention relates to a DNA base sequence, capable of increasing the amount of protein secreted by microorganisms, and its derivative sequences; a recombinant plasmid including the whole or a part of said DNA base sequence; a method for preparing the recombinant plasmid in which, when microorganisms having introduced thereinto a recombinant DNA including the desired DNA base sequence are to be separated in the process of cloning, suitable transformants can be efficiently selected by taking the amount of protein secreted out of the cells and particularly the activity of an enzyme protein as an index; and a method of microbial breeding which comprises introducing the recombinant plasmid into a microorganism to increase the amount of protein secreted by the microorganism.
    Type: Grant
    Filed: April 8, 1988
    Date of Patent: April 25, 1989
    Assignees: Agency of Industrial Science and Technology, Ministry of International Trade and Industry
    Inventors: Yoshio Furutani, Noboru Tomioka, Masaru Honjo, Kazuaki Manabe, Hiroaki Shimada
  • Patent number: 4731327
    Abstract: A recombinant DNA molecule, a portion of which includes a vector whose replication is initiated independently of the temperature-sensitive mutant gene of a temperature-sensitive mutant strain of the genus Bacillus as host is introduced into the host. The host is then cultured at a temperature which does not completely inhibit chromosomal DNA replication. By this process, the recombinant DNA molecule can be stabilized in the host and its expression can be enhanced.
    Type: Grant
    Filed: August 18, 1986
    Date of Patent: March 15, 1988
    Assignees: Agency of Industrial Science and Technology, Ministry of International Trade and Industry
    Inventors: Yoshio Furutani, Masaru Honjo, Kazuaki Manabe, Hiroaki Shimada, Noboru Tomioka
  • Patent number: 4327183
    Abstract: This invention provides a method for purifying crude fatty acid esters of saccharide containing at least fatty acid glycerides as impurity, and which method comprises decomposing the fatty acid glycerides by the treatment thereof with a lipid splitting enzyme or with a combination of a lipid splitting enzyme and a reducing agent in the presence of water.
    Type: Grant
    Filed: December 5, 1980
    Date of Patent: April 27, 1982
    Assignee: Mitsui Toatsu Chemicals, Inc.
    Inventors: Takayoshi Masuda, Masaru Honjo, Tsutomu Takase, Yoshimoto Watanabe
  • Patent number: RE45499
    Abstract: The crystalline Polymorph B of N-(2-aminophenyl)-4-[N-(pyridine-3-yl)methoxy-carbonyaminomethyl]benzamide (MS-275) of formula I is described, as well as the process for the production of said compound, and its use as a medicament for the treatment of selected diseases.
    Type: Grant
    Filed: March 7, 2014
    Date of Patent: April 28, 2015
    Assignee: Bayer Intellectual Property GmbH
    Inventors: Matthias Schneider, Michael Gottfried, Jens Geisler, Gabriele Winter, Joji Suzuki, Tomoyuki Ando, Masaru Honjo