Abstract: Embodied is a flow cell that is for analysis of nucleic acid and that is capable of inhibiting complication of data processing without a reduction in throughput. The flow cell for analysis of nucleic acid comprises: a flow path formation body that has a flow path into which a nucleic acid sample flows; and a flow path formation body support member that has a region in which nucleic acid in a nucleic acid sample flowing into the flow path is to be adsorbed. The flow path formation body support member has the nucleic acid adsorption region in which nucleic acid is to be adsorbed, and a fluorescent particle adsorption region which is separated from the nucleic acid adsorption region, and in which fluorescent particles are to be adsorbed. In the fluorescent particle adsorption region, a blocking substance that can be specifically adsorbed to the fluorescent particle adsorption region is adsorbed.

Abstract: At the positions of spots which are arranged on a substrate, image aligning is made difficult by the occurrence of a recognition error of the positions of spots, said spots being adjacent to each other in a patterned form, or a displacement caused by the expansion or deformation of the substrate due to device operation, temperature control, etc. The present invention provides: a substrate for nucleic acid analysis, on the surface of which a patterned spot area provided with spots to which a biopolymer is adhered and a randomly distributed spot area are formed; and an analysis method.

Abstract: In order to reduce the cost of producing a spot array substrate and reduce the cost of nucleic acid polymer analysis, a spot array substrate is used which is produced by preparing a resin substrate 402 having a surface on which an uneven pattern is formed and a plurality of bead sitting positions set in a two-dimensional array within the uneven pattern, and loading surface-modified beads onto the bead sitting positions of the resin substrate.

Abstract: The substrate 100 for use in the analysis of a nucleic acid according to the present invention has multiple analysis areas 12 which are partitioned on a substrate 10, and enables the measurement of the analysis areas 12 while interchanging the analysis areas 12 in turn, said substrate 100 being characterized in that each of the analysis areas 12 consists of an adsorption part 13 onto which a DNA fragment or a carrier having the DNA fragment carried thereon can be adsorbed and a non-adsorption part 14 which is a part outside of the adsorption part 13, and the non-adsorption part 14 has, formed on at least a part thereof, a marker part 15 that has a specified shape and helps to identify the positions of the analysis areas 12.

Abstract: Provided is a flow cell for nucleic acid analysis used for a sequence reaction with photocleavable nucleotides where an efficiency of the photocleaving reaction can be enhanced and noise upon florescence detection can be mitigated, thereby improving the accuracy of sequencing, shortening a runtime, and extending a read length. The flow cell for nucleic acid analysis includes a first substrate 101 provided with an optical filter 102 reflecting first light for changing a chemical structure of a substance in a flow passage, a hollow sheet 103 having a hollow portion for forming the flow passage, and a second substrate 105 transmitting the first light, in which the first substrate, the hollow sheet, and the second substrate are attached to each other.

Abstract: The substrate 100 for use in the analysis of a nucleic acid according to the present invention has multiple analysis areas 12 which are partitioned on a substrate 10, and enables the measurement of the analysis areas 12 while interchanging the analysis areas 12 in turn, said substrate 100 being characterized in that each of the analysis areas 12 consists of an adsorption part 13 onto which a DNA fragment or a carrier having the DNA fragment carried thereon can be adsorbed and a non-adsorption part 14 which is a part outside of the adsorption part 13, and the non-adsorption part 14 has, formed on at least a part thereof, a marker part 15 that has a specified shape and helps to identify the positions of the analysis areas 12.

Abstract: In order to reduce the cost of producing a spot array substrate and reduce the cost of nucleic acid polymer analysis, a spot array substrate is used which is produced by preparing a resin substrate 402 having a surface on which an uneven pattern is formed and a plurality of bead sitting positions set in a two-dimensional array within the uneven pattern, and loading surface-modified beads onto the bead sitting positions of the resin substrate.

Abstract: Sample nucleic acids are prevented from removing from a sample-immobilizing layer during a sequencing reaction. A reaction device for nucleic acid analysis is provided that enables high throughput analysis by increasing the number of nucleic acid samples that can be analyzed. The reaction device for nucleic acid analysis comprises a substrate, a sample-immobilizing layer on the substrate, nucleic acid samples or carriers having nucleic acid samples on their surfaces, which are immobilized on the sample-immobilizing layer, and a blocking layer that covers areas other than areas where the nucleic acid samples or the carriers are bound to the sample-immobilizing layer, wherein the immobilizing layer is formed of an inorganic oxide.

Abstract: Provided is a flow cell for nucleic acid analysis used for a sequence reaction with photocleavable nucleotides where an efficiency of the photocleaving reaction can be enhanced and noise upon florescence detection can be mitigated, thereby improving the accuracy of sequencing, shortening a runtime, and extending a read length. The flow cell for nucleic acid analysis includes a first substrate 101 provided with an optical filter 102 reflecting first light for changing a chemical structure of a substance in a flow passage, a hollow sheet 103 having a hollow portion for forming the flow passage, and a second substrate 105 transmitting the first light, in which the first substrate, the hollow sheet, and the second substrate are attached to each other.

Abstract: Provided is a reaction device for nucleic acid analysis wherein microparticles, which carry a nucleic acid to be detected having been immobilized thereon, are aligned in a lattice form on a substrate according to the pixel size of a two-dimensional sensor. By this reaction device for nucleic acid analysis which is provided with a channel-forming reaction chamber on the substrate (101), the nucleic acid having been immobilized on the microparticles (103) on the substrate (101) is detected. The microparticles (103), which carry the nucleic acid to be detected having been immobilized thereon, are arranged by microstructures (102) aligned on the substrate (101).

Abstract: A nucleic acid analysis reaction cell and a nucleic acid analyzer are provided, in which a uniform flow rate is realized, so that a portion where a flow rate is low is removed and washing time for reagent removal is shortened. A flow path (103) is provided which includes a detection area (108) for detecting sequence information of a nucleic acid fragment and detection outer areas (107) disposed at both ends of the detection area (108). An inflow port (105) is provided in one of the detection outer areas (107) and a discharge port (106) is provided in the other of the detection outer areas (107). The detection outer areas (107) disposed at both the ends of the detection area (108) are areas whose widths become narrow toward ends, and guides (104) for branching a liquid are provided in at least the detection outer area (107) in which the inflow port (105) is provided.

Abstract: This invention provides a biomolecule modifying substrate comprising biomolecules selectively fixed to given regions thereon. The biomolecule modifying substrate comprises: a substrate at least comprising a first surface and a second surface; a first linker molecule comprising a hydrocarbon chain and a functional group capable of selectively binding to the first surface at one end of the hydrocarbon chain, which is bound to the first surface via such functional group; a second linker molecule comprising a reactive group capable of binding to the hydrocarbon chain of the first linker molecule, which is bound to the first linker molecule via a bond between the reactive group and the hydrocarbon chain; and a biomolecule bound thereto via the second linker molecule.

Abstract: The present invention relates to a nucleic acid analysis device for analysis of nucleic acid in a sample through fluorometry, in which a localized surface plasmon by light irradiation, and in which a nucleic acid probe or a nucleic acid synthase for the analysis of the nucleic acid in the sample is disposed in a region of generation of the surface plasmon. The present invention allows the fluorescence intensifying effect of the surface plasmon to be produced efficiently and also enables the immobilization of a DNA probe or the nucleic acid synthase in a region on which the fluorescence intensifying effect is exerted, thus making it possible to carry out a measurement on the base elongation reaction without having to remove the unreacted substrate with the fluorescent molecule.

Abstract: An object of the present invention relates to distinguishing, from a fluorophore of an unreacted substrate, a single fluorophore attached to a nucleotide that is incorporated into a probe by a nucleic acid synthesis. The present invention relates to a nucleic acid analyzing device that analyzes a nucleic acid in sample by fluorescence, wherein a localized surface plasmon is generated by illumination, and a probe for analyzing the nucleic acid in the sample is on the site where the surface plasmon is generated. According to the present invention, since it is possible to efficiently produce fluorescence intensifying effects due to the surface plasmon and to immobilize the probe to a region within the reach of the fluorescence intensifying effects, it becomes possible to measure a nucleic acid synthesis without removing unreacted nucleotide to which fluorophores are attached.

Abstract: Sample nucleic acids are prevented from removing from a sample-immobilizing layer during a sequencing reaction. A reaction device for nucleic acid analysis is provided that enables high throughput analysis by increasing the number of nucleic acid samples that can be analyzed. The reaction device for nucleic acid analysis comprises a substrate, a sample-immobilizing layer on the substrate, nucleic acid samples or carriers having nucleic acid samples on their surfaces, which are immobilized on the sample-immobilizing layer, and a blocking layer that covers areas other than areas where the nucleic acid samples or the carriers are bound to the sample-immobilizing layer, wherein the immobilizing layer is formed of an inorganic oxide.

Abstract: A nucleic acid analysis reaction cell and a nucleic acid analyzer are provided, in which a uniform flow rate is realised, so that a portion where a flow rate is low is removed and washing time for reagent removal is shortened. A flow path (103) is provided which includes a detection area (108) for detecting sequence information of a nucleic acid fragment and detection outer areas (107) disposed at both ends of the detection area (108). An inflow port (105) is provided in one of the detection outer areas (107) and a discharge port (106) is provided in the other of the detection outer areas (107). The detection outer areas (107) disposed at both the ends of the detection area (108) are areas whose widths become narrow toward ends, and guides (104) for branching a liquid are provided in at least the detection outer area (107) in which the inflow port (105) is provided.

Abstract: Provided is a reaction device for nucleic acid analysis wherein microparticles, which carry a nucleic acid to be detected having been immobilized thereon, are aligned in a lattice form on a substrate according to the pixel size of a two-dimensional sensor. By this reaction device for nucleic acid analysis which is provided with a channel-forming reaction chamber on the substrate (101), the nucleic acid having been immobilized on the microparticles (103) on the substrate (101) is detected. The microparticles (103), which carry the nucleic acid to be detected having been immobilized thereon, are arranged by microstructures (102) aligned on the substrate (101).

Abstract: An object of the present invention relates to detecting a target substance with high contrast. The invention relates to analysis of a target substance using a light-transmitting substrate and a metal for inducing plasmon resonance, and further using a low refractive index layer with an opening portion, which forms an interface with the substrate, and which has a lower refractive index than the substrate. Light emitted from a substrate side is totally reflected at the interface to irradiate the metal arranged in the opening portion with evanescent light. Light generated from the target substance by plasmon resonance of the evanescent light is detected. According to the invention, the radiation of evanescent light to a material other than the target substance can be reduced, and thereby light emission from the martial other than the target substance, e.g., a molecule floating around the target substance, can be reduced.

Abstract: This invention provides a biomolecule modifying substrate comprising biomolecules selectively fixed to given regions thereon. The biomolecule modifying substrate comprises: a substrate at least comprising a first surface and a second surface; a first linker molecule comprising a hydrocarbon chain and a functional group capable of selectively binding to the first surface at one end of the hydrocarbon chain, which is bound to the first surface via such functional group; a second linker molecule comprising a reactive group capable of binding to the hydrocarbon chain of the first linker molecule, which is bound to the first linker molecule via a bond between the reactive group and the hydrocarbon chain; and a biomolecule bound thereto via the second linker molecule.

Abstract: An object of the present invention relates to distinguishing, from a fluorophore of an unreacted substrate, a single fluorophore attached to a nucleotide that is incorporated into a probe by a nucleic acid synthesis. The present invention relates to a nucleic acid analyzing device that analyzes a nucleic acid in sample by fluorescence, wherein a localized surface plasmon is generated by illumination, and a probe for analyzing the nucleic acid in the sample is on the site where the surface plasmon is generated. According to the present invention, since it is possible to efficiently produce fluorescence intensifying effects due to the surface plasmon and to immobilize the probe to a region within the reach of the fluorescence intensifying effects, it becomes possible to measure a nucleic acid synthesis without removing unreacted nucleotide to which fluorophores are attached.