Abstract: A method for detecting HER2-positive cancer cells, comprising steps of: (a) fixing and then permeabilizing cells; (b) contacting a primary antibody that recognizes an intracellular epitope of HER2 with the cells and then contacting a secondary antibody that recognizes the primary antibody and is labeled with a fluorescent dye; and (c) irradiating the cells with excitation light for the fluorescent dye and detecting fluorescence emitted from the cells is provided. According to such a method, HER2-positive cancer cells can be detected with high sensitivity.

Abstract: The present invention provides a method for detecting circulating tumor cells, comprising steps of: capturing cells in a blood sample on a filter; contacting an antibody that is labeled with a first fluorescent dye and recognizes a leukocyte marker protein with the cell-captured filter; contacting an animal serum, a surfactant and an antibody that is labeled with a second fluorescent dye and recognizes an epithelial cell marker protein with the cell-captured filter; contacting a third fluorescent dye that stains nucleic acids with the cell-captured filter; and detecting fluorescences emitted from each cell captured on the filter.

Abstract: The present invention provides a method for capturing rare cells, comprising a step of adding beads to a fluid containing rare cells and contaminating cells; and a step of filtering, with a filter, the fluid to which the beads have been added. According to this method, rare cells can be enriched with the rare cells selectively captured from a fluid containing the rare cells and contaminating cells.

Abstract: Provided is a strip-shaped detector that detects an analyte in a liquid sample. The detector includes a collecting member that directly collects a liquid sample from a living organism, a holding member that holds a labeling reagent binding specifically to the analyte in a state where the labeling reagent can move along with the movement of the liquid sample, a detecting member to which a detection reagent is immobilized which captures a complex of the analyte and the labeling reagent by binding specifically to the analyte, an absorbing member that can absorb the liquid sample, and a liquid-impermeable supporting member, wherein the respective members are arranged on the supporting member in the longitudinal direction of the detector so that the liquid sample moves through the inside of these members, and the collecting member includes a protruding portion sticking out of the supporting member at the upstream side in the movement direction of the liquid sample.

Abstract: The present invention provides a method for analyzing agonist-activity to a cytokinin receptor, which comprises (1) bringing an examinee substance into contact with a transformed cell into which DNA coding the cytokinin receptor is introduced and (2) measuring the existence or the quantity of intracellular signal transduction from the cytokinin receptor expressed in the transformed cell, and, a method for analyzing antagonist-activity to a cytokinin receptor, which comprises (1) bringing an examinee substance and a substance having agonist-activity to the cytokinin receptor into contact with a transformed cell into which DNA coding the cytokinin receptor is introduced.

Abstract: Provided is a strip-shaped detector that detects an analyte in a liquid sample. The detector includes a collecting member that directly collects a liquid sample from a living organism, a holding member that holds a labeling reagent binding specifically to the analyte in a state where the labeling reagent can move along with the movement of the liquid sample, a detecting member to which a detection reagent is immobilized which captures a complex of the analyte and the labeling reagent by binding specifically to the analyte, an absorbing member that can absorb the liquid sample, and a liquid-impermeable supporting member, wherein the respective members are arranged on the supporting member in the longitudinal direction of the detector so that the liquid sample moves through the inside of these members, and the collecting member includes a protruding portion sticking out of the supporting member at the upstream side in the movement direction of the liquid sample.

Abstract: The present invention provides a method for analyzing agonist-activity to a cytokinin receptor, which comprises (1) bringing an examinee substance into contact with a transformed cell into which DNA coding the cytokinin receptor is introduced and (2) measuring the existence or the quantity of intracellular signal transduction from the cytokinin receptor expressed in the transformed cell, and, a method for analyzing antagonist activity to a cytokinin receptor, which comprises (1) bringing an examinee substance and a substance having agonist-activity to the cytokinin receptor into contact with a transformed cell into which DNA coding the cytokinin receptor is introduced and (2) measuring the existence or the quantity of intracellular signal transduction from the cytokinin receptor expressed in the transformed cell, and the like.

Abstract: The present invention provides a method for analyzing agonist-activity to a cytokinin receptor, which comprises (1) bringing an examinee substance into contact with a transformed cell into which DNA coding the cytokinin receptor is introduced and (2) measuring the existence or the quantity of intracellular signal transduction from the cytokinin receptor expressed in the transformed cell, and, a method for analyzing antagonist activity to a cytokinin receptor, which comprises (1) bringing an examinee substance and a substance having agonist-activity to the cytokinin receptor into contact with a transformed cell into which DNA coding the cytokinin receptor is introduced and (2) measuring the existence or the quantity of intracellular signal transduction from the cytokinin receptor expressed in the transformed cell, and the like.

Abstract: The present invention provides a method for analyzing agonist-activity to a cytokinin receptor, which comprises (1) bringing an examinee substance into contact with a transformed cell into which DNA coding the cytokinin receptor is introduced and (2) measuring the existence or the quantity of intracellular signal transduction from the cytokinin receptor expressed in the transformed cell, and, a method for analyzing antagonist-activity to a cytokinin receptor, which comprises (1) bringing an examinee substance and a substance having agonist-activity to the cytokinin receptor into contact with a transformed cell into which DNA coding the cytokinin receptor is introduced and (2) measuring the existence or the quantity of intracellular signal transduction from the cytokinin receptor expressed in the transformed cell, and the like.

Abstract: An AMLCD having high fineness and high contrast is realized. First, an interlayer film is provided on an element electrode, and an opening portion is formed in the interlayer film. Next, after a first metal layer is formed, an embedded insulating layer is formed. The embedded insulating layer is retreated by a means, such as an etch back method, to realize a state in which only the opening portion is filled with the embedded insulating layer. By this, electric connection between the element electrode and a second metal layer becomes possible while keeping the flatness.

Abstract: The present invention provides a transformed cell co-expressing a cytokinin receptor and a cytokinin biosynthesis enzyme and a method for analyzing the activity of controlling biosynthesis of cytokinins using such a transformed cell.

Abstract: An AMLCD having high fineness and high contrast is realized. First, an interlayer film is provided on an element electrode, and an opening portion is formed in the interlayer film. Next, after a first metal layer is formed, an embedded insulating layer is formed. The embedded insulating layer is retreated by a means, such as an etch back method, to realize a state in which only the opening portion is filled with the embedded insulating layer. By this, electric connection between the element electrode and a second metal layer becomes possible while keeping the flatness.

Abstract: An AMLCD having high fineness and high contrast is realized. First, an interlayer film is provided on an element electrode, and an opening portion is formed in the interlayer film. Next, after a first metal layer is formed, an embedded insulating layer is formed. The embedded insulating layer is retreated by a means, such as an etch back method, to realize a state in which only the opening portion is filled with the embedded insulating layer. By this, electric connection between the element electrode and a second metal layer becomes possible while keeping the flatness.

Abstract: The present invention provides a method for analyzing agonist-activity to a cytokinin receptor, which comprises

Abstract: A remotely programmable radiotelephone and a remote programming unit. The radiotelephone (10) includes a receiver front end and demodulator (23), receiver signal processing circuits (30), a dual-tone, multiple-frequency converter (31), a controller (50), and a memory (51). The converter (31) converts DTMF tones into digital signals. The controller (50) monitors the output of the converter (31). If the output corresponds to a valid command then the controller (50) executes the command. Certain commands must be preceded by the user entering an access code using a control keypad (56) and by the reception of DTMF tones corresponding to a password or security code stored in the memory (51). Some commands cause telephone numbers to be stored in the memory (51) for later recall by the user. Other commands cause a number to be stored and an outgoing call to be automatically initiated.

Abstract: An electronic apparatus comprises a random access memory, a central processing unit which supplies a write/read signal to the random access memory to control the input/output of data of the random access memory, a replaceable power source for feeding the central processing unit, and a switch for isolating the random access memory from the write or read signal output from the central processing unit when the power source is replaced.

Abstract: There is disclosed an electronic assembly for use in calculators, watches and so forth. The assembly includes an integrated circuit element having a plurality of positioning apertures at the periphery thereof and a circuit board (preferably, a flexible circuit film) having a plurality of terminals to be electrically connected to the respective terminals of the integrated circuit element and also having a plurality of positioning apertures. The assembly further includes an upper casing and a lower casing one of which has a plurality of positioning projections to be received within the positioning apertures in the circuit element and a plurality of positioning projections to be received within the positioning apertures in the circuit board, for determining relative position of the integrated circuit element and the circuit board with respect to the casing.

Abstract: There is disclosed an electronic assembly for use in calculators, watches and so forth. The assembly includes an integrated circuit element having a plurality of positioning apertures at the periphery thereof and a circuit board (preferably, a flexible circuit film) having a plurality of terminals to be electrically connected to the respective terminals of the integrated circuit element and also having a plurality of positioning apertures. The assembly further includes an upper casing and a lower casing one of which has a plurality of positioning projections to be received within the positioning apertures in the circuit element and a plurality of positioning projections to be received within the positioning apertures in the circuit board, for determining relative position of the integrated circuit element and the circuit board with respect to the casing.

Abstract: There is disclosed an electronic assembly for use in calculators, watches and so forth. The assembly includes an integrated circuit element having a plurality of positioning apertures at the periphery thereof and a circuit board (preferably, a flexible circuit film) having a plurality of terminals to be electrically connected to the respective terminals of the integrated circuit element and also having a plurality of positioning apertures. The assembly further includes an upper casing and a lower casing one of which has a plurality of positioning projections to be received within the positioning apertures in the circuit element and a plurality of positioning projections to be received within the positioning apertures in the circuit board, for determining relative position of the integrated circuit element and the circuit board with respect to the casing.