Patents by Inventor Mats Gullberg
Mats Gullberg has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Patent number: 11788119Abstract: Multiplexed methods of detecting an analyte in a sample using two or more padlock probes each specific to a different target sequence are described. The target sequence is either part of an analyte or indicative of the presence of an analyte in the sample. Each padlock probe includes an analyte-specific reporter sequence, and either a restriction cleavage site located 3? of the analyte-specific reporter sequence, and/or a first amplification primer binding site for an amplification reaction. Where the padlock probe includes a restriction cleavage site, cleavage at the restriction cleavage site occurs 3? of the analyte-specific reporter sequence. Where the padlock probe includes a first amplification primer binding site for the further amplification reaction, it does not contain a second amplification primer binding site 5? of the analyte-specific reporter sequence. Panels of probes and kits for the same are also described.Type: GrantFiled: December 15, 2017Date of Patent: October 17, 2023Assignee: Q-Linea ABInventors: Camilla Russel, Jenny Goransson, Mats Gullberg
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Publication number: 20220195490Abstract: The present invention provides a method of recovering viable microbial cells from a complex sample, said method comprising: a) providing a sample having a volume of at least 1 ml; b) contacting said sample with a buffer solution and one or more proteases, wherein said buffer solution has a pH of at least pH 6 and less than pH 11, wherein said buffer solution and said one more proteases do not comprise a detergent or a chaotrope, and wherein the buffer solution/protease/sample mixture is non-hypotonic; c) filtering the mixture obtained in step (b) through a filter suitable for retaining microbial cells; and d) recovering the microbial cells retained by the filter in step (c), wherein the recovered microbial cells are viable, and a microbial recovery device for the same.Type: ApplicationFiled: January 19, 2022Publication date: June 23, 2022Applicant: Q-linea ABInventors: Markus Klintstedt, Harer Osman, Mats Gullberg
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Patent number: 11261476Abstract: The present invention provides a method of recovering viable microbial cells from a complex sample, said method comprising: a) providing a sample having a volume of at least 1 ml; b) contacting said sample with a buffer solution and one or more proteases, wherein said buffer solution has a pH of at least pH 6 and less than pH 11, wherein said buffer solution and said one more proteases do not comprise a detergent or a chaotrope, and wherein the buffer solution/protease/sample mixture is non-hypotonic; c) filtering the mixture obtained in step (b) through a filter suitable for retaining microbial cells; and d) recovering the microbial cells retained by the filter in step (c), wherein the recovered microbial cells are viable, and a microbial recovery device for the same.Type: GrantFiled: October 19, 2017Date of Patent: March 1, 2022Assignee: Q-LINEA ABInventors: Markus Klintstedt, Harer Osman, Mats Gullberg
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Publication number: 20210017568Abstract: The present invention provides methods for determining the antimicrobial susceptibility of a microorganism in a clinical sample said method comprising removing a test aliquot from a clinical sample culture before the culture reaches 0.5 McFarland units, isolating the microbial cells and transferring the cells into a suitable culture medium for microbial growth, and performing an AST assay using the isolated microbes, wherein the concentration of microbial cells in the microbial cells used to set up the AST assay is measured before the degree of microbial growth in the different growth conditions of the AST assay is measured. Devices for determining the antimicrobial susceptibility of a microorganism in a clinical sample are also provided.Type: ApplicationFiled: October 1, 2020Publication date: January 21, 2021Applicant: Q-linea ABInventors: Jonas Jarvius, Jan Grawe, Ylva Molin, Markus Klintstedt, Mats Gullberg
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Patent number: 10829797Abstract: A method for determining the antimicrobial susceptibility of a microorganism in a clinical sample includes removing a test aliquot from a clinical sample culture before the culture reaches 0.5 McFarland units and isolating the microbial cells. The cells are transferred into a suitable culture medium for microbial growth and an AST assay is performed using the isolated microbes. The concentration of microbial cells in the microbial cells used to set up the AST assay is measured before measuring the degree of microbial growth in different conditions of the AST assay. Devices for determining the anti-microbial susceptibility of a microorganism in a clinical sample are also disclosed.Type: GrantFiled: June 16, 2016Date of Patent: November 10, 2020Assignee: Q-linea ABInventors: Jonas Jarvius, Jan Grawe, Ylva Molin, Markus Klintstedt, Mats Gullberg
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Patent number: 10669569Abstract: Methods for detecting and quantifying an analyte employ a pair of proximity probes, each comprising a proteinaceous target-binding domain coupled to a nucleic acid domain (NAD), which NADs interact when the proximity probes have bound in proximity to their respective target; and a set of markers, wherein each marker is a nucleic acid molecule comprising a binding domain and a reporter domain giving a detectable signal, can interact with said NADs to form a nucleic acid molecule from which a detectable signal is generated, or with a nucleic acid molecule generated by interaction of said NADs, cannot interact with said NADs simultaneously with another marker in the set, generates a signal that is distinguishable from another marker signal, and is present in an amount capable of detecting analyte at a range of concentrations differing from the range of concentrations detectable by other markers.Type: GrantFiled: October 14, 2011Date of Patent: June 2, 2020Assignee: Navinci Diagnostics ABInventors: Mats Gullberg, Irene Weibrecht, Carl-Magnus Clausson, Ola Söderberg
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Publication number: 20200040376Abstract: Multiplexed methods of detecting an analyte in a sample using two or more padlock probes each specific to a different target sequence are described. The target sequence is either part of an analyte or indicative of the presence of an analyte in the sample. Each padlock probe includes an analyte-specific reporter sequence, and either a restriction cleavage site located 3? of the analyte-specific reporter sequence, and/or a first amplification primer binding site for an amplification reaction. Where the padlock probe includes a restriction cleavage site, cleavage at the restriction cleavage site occurs 3? of the analyte-specific reporter sequence. Where the padlock probe includes a first amplification primer binding site for the further amplification reaction, it does not contain a second amplification primer binding site 5? of the analyte-specific reporter sequence. Panels of probes and kits for the same are also described.Type: ApplicationFiled: December 15, 2017Publication date: February 6, 2020Applicant: Q-linea ABInventors: Camilla Russel, Jenny Goransson, Mats Gullberg
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Patent number: 10465235Abstract: The present invention provides a method for detecting interactions between or with any two of at least three target substrates, or any two of at least three features of a target substrate, or a combination of interactions and features of target substrates, by a multiplexed proximity ligation assay, said method comprising: a) for each of the at least three target substrates or features, providing a proximity probe comprising a binding moiety with affinity for the feature or binding site on said substrate, and a proximity probe oligonucleotide coupled on the binding moiety; wherein each of the proximity probe oligonucleotide carries a unique tag sequence; b) mixing the proximity probes with a sample, under a condition to allow binding of each proximity probe to its respective binding site or feature on each of said substrates through the binding moiety, c) simultaneous with, or following step b), forming circularized DNA molecules where any two proximity probes bind sufficiently close to each other on the substType: GrantFiled: May 23, 2012Date of Patent: November 5, 2019Assignee: NAVINCI DIAGNOSTICS ABInventors: Mats Gullberg, Ola Söderberg, Ulf Landegren, Yanling Liu
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Publication number: 20190316173Abstract: The present invention provides a method of recovering viable microbial cells from a complex sample, said method comprising: a) providing a sample having a volume of at least 1 ml; b) contacting said sample with a buffer solution and one or more proteases, wherein said buffer solution has a pH of at least pH 6 and less than pH 11, wherein said buffer solution and said one more proteases do not comprise a detergent or a chaotrope, and wherein the buffer solution/protease/sample mixture is non-hypotonic; c) filtering the mixture obtained in step (b) through a filter suitable for retaining microbial cells; and d) recovering the microbial cells retained by the filter in step (c), wherein the recovered microbial cells are viable, and a microbial recovery device for the same.Type: ApplicationFiled: October 19, 2017Publication date: October 17, 2019Applicant: Q-linea ABInventors: Markus Klintstedt, Harer Osman, Mats Gullberg
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Publication number: 20180223327Abstract: The present invention provides methods for determining the antimicrobial susceptibility of a microorganism in a clinical sample said method comprising removing a test aliquot from a clinical sample culture before the culture reaches 0.5 McFarland units, isolating the microbial cells and transferring the cells into a suitable culture medium for microbial growth, and performing an AST assay using the isolated microbes, wherein the concentration of microbial cells in the microbial cells used to set up the AST assay is measured before the degree of microbial growth in the different growth conditions of the AST assay is measured. Devices for determining the antimicrobial susceptibility of a microorganism in a clinical sample are also provided.Type: ApplicationFiled: June 16, 2016Publication date: August 9, 2018Applicant: Q-linea ABInventors: Jonas Jarvius, Jan Grawe, Ylva Molin, Markus Klintstedt, Mats Gullberg
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Publication number: 20140194311Abstract: The present invention provides a method for detecting interactions between or with any two of at least three target substrates, or any two of at least three features of a target substrate, or a combination of interactions and features of target substrates, by a multiplexed proximity ligation assay, said method comprising: a) for each of the at least three target substrates or features, providing a proximity probe comprising a binding moiety with affinity for the feature or binding site on said substrate, and a proximity probe oligonucleotide coupled on the binding moiety; wherein each of the proximity probe oligonucleotide carries a unique tag sequence; b) mixing the proximity probes with a sample, under a condition to allow binding of each proximity probe to its respective binding site or feature on each of said substrates through the binding moiety, c) simultaneous with, or following step b), forming circularized DNA molecules where any two proximity probes bind sufficiently close to each other on the substType: ApplicationFiled: May 23, 2012Publication date: July 10, 2014Applicant: OLINK ABInventors: Mats Gullberg, Ola Söderberg, Ulf Landegren, Yangling Liu
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Publication number: 20130288249Abstract: The present invention relates to methods for detecting and quantifying an analyte in a sample, principally in proximity probe assays, and in particular to an improvement in such methods to extend the dynamic range of detection, which is particularly advantageous for the detection and quantification of an analyte where the concentration range of the analyte in said sample is unknown and/or the range is likely to be broad, said method comprising: (i) contacting said sample with at least a pair of proximity probes each comprising a proteinaceous target-binding domain coupled to a nucleic acid domain such that said nucleic acid domains may be allowed to interact directly or indirectly when the proximity probes have bound in proximity to their respective target, said target being either the analyte or a binding partner for the analyte; (ii) further contacting said sample with at least one set of markers which function to extend the dynamic range of detection of the method, wherein said set comprises at least two mType: ApplicationFiled: October 14, 2011Publication date: October 31, 2013Applicant: OLINK ABInventors: Mats Gullberg, Irene Weibrecht, Carl-Magnus Clausson, Ola Söderberg
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Patent number: 8053188Abstract: This invention relates to methods, reagents and kits for enriching nucleic acid sequences. More particularly, the present invention relates to methods, reagents and kits for sample preparation including sample modification, sample enrichment and amplification.Type: GrantFiled: November 20, 2002Date of Patent: November 8, 2011Assignee: Olink ABInventors: Mats Gullberg, Ulf Landegren
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Publication number: 20110223585Abstract: The present invention relates to a method for detecting an analyte in a sample, said method comprising: (a) contacting said sample with at least one set of at least first and second proximity probes, wherein said probes each comprise an analyte-binding moiety and can simultaneously bind to the analyte, and wherein (i) said first proximity probe comprises a nucleic acid moiety attached at one end to the analyte-binding moiety, wherein a circular or circularizable oligonucleotide is hybridized to said nucleic acid moiety before, during or after said contacting step; and (ii) said second proximity probe comprises an enzyme moiety, attached to the analyte-binding moiety, capable of directly or indirectly enabling rolling circle amplification (RCA) of the circular or, when it is circularized, of the circularizable oligonucleotide hybridized to the nucleic acid moiety of the first proximity probe, wherein said RCA is primed by said nucleic acid moiety of said first proximity probe; (b) if necessary, circularizing sType: ApplicationFiled: March 10, 2011Publication date: September 15, 2011Applicant: OLINK ABInventors: MATS GULLBERG, SIMON FREDRIKSSON
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Patent number: 7790388Abstract: A nucleic acid amplification method, and probes for use within the method are described.Type: GrantFiled: November 28, 2007Date of Patent: September 7, 2010Assignee: Olink ABInventors: Ulf Landegren, Mats Gullberg, Mats Nilsson
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Publication number: 20080131899Abstract: A nucleic acid amplification method, and probes for use within the method are described.Type: ApplicationFiled: November 28, 2007Publication date: June 5, 2008Applicant: OLINK A.B.Inventors: Ulf Landegren, Mats Gullberg, Mats Nilsson
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Patent number: 7320860Abstract: A nucleic acid amplification method, and probes for use within the method are described.Type: GrantFiled: July 12, 2002Date of Patent: January 22, 2008Assignee: Olink A.B.Inventors: Ulf Landegren, Mats Gullberg, Mats Nilsson
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Publication number: 20050287526Abstract: A nucleic acid amplification method, and probes for use within the method are described.Type: ApplicationFiled: July 12, 2002Publication date: December 29, 2005Applicants: Biocyclica AB, Landegren Gene Technology ABInventors: Ulf Landegren, Mats Gullberg, Mats Nilsson
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Patent number: RE44265Abstract: A nucleic acid amplification method, and probes for use within the method are described.Type: GrantFiled: December 19, 2011Date of Patent: June 4, 2013Assignee: Olink ABInventors: Ulf Landegren, Mats Gullberg
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Patent number: D1016317Type: GrantFiled: November 19, 2020Date of Patent: February 27, 2024Assignee: Q-LINEA ABInventors: Jonas Jarvius, Jan Grawé, Mats Gullberg, Jerker Persson, Karin Granström