Patents by Inventor Mattias Karlsson

Mattias Karlsson has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Publication number: 20110183407
    Abstract: Apparatuses and methods of aligning at least one tip of a tip manifold with a plurality of wells of a multiwell plate. The tip manifold includes a plate, at least one tip depending from the plate, a first tip alignment pin depending from the plate, and a second tip alignment pin depending from the plate. The second tip alignment pin opposes the first tip alignment pin. The multiwell plate includes a body defining a plurality of non-porous wells for holding biological material, a first alignment hole, and a second alignment hole. The second alignment hole opposes the first alignment hole.
    Type: Application
    Filed: March 10, 2009
    Publication date: July 28, 2011
    Applicant: CELLECTRICON AB
    Inventors: Johan Pihl, Mattias Karlsson
  • Publication number: 20110091864
    Abstract: Disclosed herein is a device comprising at least one supporting solid surface comprising at least one membraneophilic region; a covering layer that is at least partially immobilized to the membraneophilic region, said covering layer consisting of (i) a surfactant membrane, (ii) a lipid mimicking polymer, (iii) a surfactant or emulsion system or (iv) a liquid crystal; and a substance included in or bound to, connected to or associated with the covering layer. Also disclosed are methods wherein the device is used and use of the device.
    Type: Application
    Filed: December 23, 2005
    Publication date: April 21, 2011
    Inventors: Anders Karlsson, Roger Karlsson, Johan Pihl, Mattias Karlsson, Owe Orwar, Tatiana Lobovkina, Max Davidson, Aldo Jesorka
  • Publication number: 20100159439
    Abstract: Disclosed is a method for sequential delivery of agents to and/or into a cell structure, wherein an electrolyte-filled tube is provided together with a counter electrode, said tube is connected to a voltage or current generator, at least two agents are introduced in a discrete mode into the electrolyte solution contained in the tube, which is placed close to the cell structure, one agent at the time being transported through the tube to and/or into said cell structure in which a pore has been formed by application of an electric field focused on the cell structure, resulting in electroporation of the cell structure. Also different applications of the method is disclosed, e.g. us of the method in order to transfer cell-impermeant solutes, such as drugs or genes, into the cell structure or out of the cell structure.
    Type: Application
    Filed: January 21, 2009
    Publication date: June 24, 2010
    Applicant: Cellectricon AB
    Inventors: Owe Orwar, Mattias Karlsson, Cecilia Farre, Kerstin Nolkrantz
  • Publication number: 20100143944
    Abstract: The invention provides microfluidic systems for altering the solution environment around a nanoscopic or microscopic object, such as a sensor, and methods for using the same. The invention can be applied in any sensor technology in which the sensing element needs to be exposed rapidly, sequentially, and controllably, to a large number of different solution environments whose characteristics may be known or unknown.
    Type: Application
    Filed: February 25, 2009
    Publication date: June 10, 2010
    Applicant: Cellectricon AB
    Inventors: Owe Orwar, Daniel Chiu, Johan Pihl, Jon Sinclair, Jessica Olofsson, Mattias Karlsson, Kent Jardemark
  • Publication number: 20090286297
    Abstract: Apparatuses and methods of aligning at least one tip of a tip manifold with a plurality of wells of a multiwell plate. The tip manifold includes a plate, at least one tip depending from the plate, a first tip alignment pin depending from the plate, and a second tip alignment pin depending from the plate. The second tip alignment pin opposes the first tip alignment pin. The multiwell plate includes a body defining a plurality of non-porous wells for holding biological material, a first alignment hole, and a second alignment hole. The second alignment hole opposes the first alignment hole.
    Type: Application
    Filed: March 10, 2009
    Publication date: November 19, 2009
    Applicant: CELLECTRICON AB
    Inventors: Johan Pihl, Mattias Karlsson
  • Patent number: 7563614
    Abstract: The invention provides microfluidic systems for altering the solution environment around a nanoscopic or microscopic object, such as a sensor, and methods for using the same. The invention can be applied in any sensor technology in which the sensing element needs to be exposed rapidly, sequentially, and controllably, to a large number of different solution environments whose characteristics may be known or unknown.
    Type: Grant
    Filed: January 15, 2003
    Date of Patent: July 21, 2009
    Assignee: Cellectricon AB
    Inventors: Owe Orwar, Daniel Chiu, Johan Pihl, Jon Sinclair, Jessica Olofsson, Mattias Karlsson, Kent Jardemark
  • Publication number: 20090092963
    Abstract: Disclosed is a method for parallel delivery of agents to and/or into a cell structure, wherein at least two electrolyte-filled tubes are provided together with a counter electrode, the tubes being connected to a voltage or current generator, said agents being introduced into the electrolyte solution contained in the tubes, which are placed close to the cell structure, whereupon the agents are transported through the tubes to said cell structure and into the said structure through pores which have been formed by application of an electric field focused on the cell structure, resulting in electroporation of the cell structure. Also different applications of the method is disclosed, e.g. use of the method in order to transfer cell-impermeant solutes, such as drugs or genes, into the cell structure or out of the cell structure.
    Type: Application
    Filed: June 9, 2008
    Publication date: April 9, 2009
    Applicant: Cellectricon AB
    Inventors: Owe Orwar, Mattias Karlsson, Kerstein Nolkrantz, Cecilia Farre
  • Publication number: 20090047676
    Abstract: The invention provides a system, system components, and a method for rapidly obtaining and stably maintaining a cell in optimal contact with the cell-contacting surface of a sensor in a cell-based biosensor. In one aspect, the system maximizes the seal between a whole cell and the cell-contact surface of a patch clamp micropipette, maximizing the efficiency of a whole cell patch clamp recording.
    Type: Application
    Filed: June 6, 2008
    Publication date: February 19, 2009
    Applicant: Cellectricon AB
    Inventors: Mattias Karlsson, Owe Orwar, Daniel T. Chiu, Jon Sinclair, Kent Jardemark, Jessica Olofsson, Johan Pihl, Cecilia Farre
  • Patent number: 7470518
    Abstract: The invention provides microfluidic systems for altering the solution environment around a nanoscopic or microscopic object, such as a sensor, and methods for using the same. The invention also provides a system and methods for modulating, controlling, preparing, and studying receptors.
    Type: Grant
    Filed: January 6, 2005
    Date of Patent: December 30, 2008
    Assignee: Cellectricon AB
    Inventors: Daniel Chiu, Owe Orwar, Kent Jardemark, Mattias Karlsson, Jessica Olofsson, Johan Pihl, Jon Sinclair
  • Patent number: 7393680
    Abstract: Disclosed is a method for penetration of lipid bilayer membranes in order to insert at the tip of a hollow needle-shaped object, such as a micropipet-, into a container formed of a lipid bilayer membrane, wherein said container is placed between said needle-shaped object, with the tip of said needle-shaped object placed in contact with said conainer in such a way that it applies a mechanical force to the lipid membrane of said container, thus mechanically straining it, and a second electrode, whereupon a transient electric pulse of 1-to-103 V/cm is applied between the electrodes, resulting in a focused electrical field over said container C which induces a dielectric breakdown of the lipid bilayer causing the needle-shaped object to penetrate the container. Disclosed is also an electroinjection method based on the above method, wherein substances are introduced through the needle-shaped object and into the container after penetration of the needle-shaped object.
    Type: Grant
    Filed: October 19, 2001
    Date of Patent: July 1, 2008
    Assignee: Cellectricon AB
    Inventors: Owe Orwar, Mattias Karlsson, Daniel Chiu
  • Patent number: 7390650
    Abstract: The invention provides a system, system components, and a method for rapidly obtaining and stably maintaining a cell in optimal contact with the cell-contacting surface of a sensor in a cell-based biosensor. In one aspect, the system maximizes the seal between a whole cell and the cell-contact surface of a patch clamp micropipette, maximizing the efficiency of a whole cell patch clamp recording.
    Type: Grant
    Filed: August 20, 2003
    Date of Patent: June 24, 2008
    Assignee: Cellectricon AB
    Inventors: Mattias Karlsson, Owe Orwar, Daniel T. Chiu, Jon Sinclair, Kent Jardemark, Jessica Olofsson, Johan Pihl, Cecilia Farre
  • Publication number: 20060234298
    Abstract: The invention provides microfluidic systems for altering the solution environment around a nanoscopic or microscopic object, such as a sensor, and methods for using the same. The invention also provides a system and methods for modulating, controlling, preparing, and studying receptors.
    Type: Application
    Filed: January 6, 2005
    Publication date: October 19, 2006
    Inventors: Daniel Chiu, Owe Orwar, Kent Jardemark, Mattias Karlsson, Jessica Olofsson, Johan Pihl, Jon Sinclair
  • Publication number: 20060223164
    Abstract: The invention provides microfluidic systems for altering the solution environment around a nanoscopic or microscopic object, such as a sensor, and methods for using the same. The invention can be applied in any sensor technology in which the sensing element needs to be exposed rapidly, sequentially, and controllably, to a large number of different solution environments whose characteristics may be known or unknown.
    Type: Application
    Filed: January 15, 2003
    Publication date: October 5, 2006
    Inventors: Owe Orwar, Daniel Chiu, Johan Pihl, Jon Sinclair, Jessica Olofsson, Mattias Karlsson, Kent Jardemark
  • Publication number: 20060078961
    Abstract: The invention provides microfluidic systems for altering the solution environment around a nanoscopic or microscopic object, such as a sensor, and methods for using the same. The invention can be applied in any sensor technology in which the sensing element needs to be exposed rapidly, sequentially, and controllably, to a large number of different solution environments whose characteristics may be known or unknown.
    Type: Application
    Filed: January 14, 2003
    Publication date: April 13, 2006
    Inventors: Daniel Chiu, Owe Orwar, Kent Jardemark, Mattias Karlsson, Jessica Olofsson, Johan Pihl, Jon Sinclair
  • Patent number: 7018819
    Abstract: An apparatus and method are disclosed for electromanipulation of at least one cell or cell-like structure having cell-like membranes, the method comprising the steps: (a) at least one cell or cell-like structure is transported from one or more sample containers located on a chip through microchannel(s) located on said chip into a chamber located on said chip, wherein said chamber contains electrode(s) connected to a voltage generator, wherein said microchannel provides a fluid contact between the sample containers, (b) said cell or cell-like structure(s) is placed close to said at least one electrode, and (c) an electrical field is applied and focused on said cell or cell-like structure(s), said electrical field being of a strength sufficient to obtain pore-formation or fusion of said at least one cell or cell-like structure with another cell or cell-like structure(s) present in said chamber.
    Type: Grant
    Filed: November 30, 2001
    Date of Patent: March 28, 2006
    Assignee: Cellectricon AB
    Inventors: Owe Orwar, Mattias Karlsson, Daniel Chiu, Anette Stromberg, Anders Karlsson
  • Publication number: 20050026283
    Abstract: Disclosed is a method for parallel delivery of agents to and/or into a cell structure, wherein at least two electrolyte-filled tubes are provided together with a counter electrode, the tubes being connected to a voltage or current generator, said agents being introduced into the electrolyte solution contained in the tubes, which are placed close to the cell structure, whereupon the agents are transported through the tubes to said cell structure and into the said structure through pores which have been formed by application of an electric field focused on the cell structure, resulting in electroporation of the cell structure. Also different applications of the method is disclosed, e.g. use of the method in order to transfer cell-impermeant solutes, such as drugs or genes, into the cell structure or out of the cell structure.
    Type: Application
    Filed: November 27, 2002
    Publication date: February 3, 2005
    Inventors: Owe Ormar, Mattias Karlsson, Kerstin Nolkrantz, Cecilia Farre
  • Publication number: 20050019921
    Abstract: Disclosed is a method for sequential delivery of agents to and/or into a cell structure, wherein an electrolyte-filled tube is provided together with a counter electrode, said tube is connected to a voltage or current generator, at least two agents are introduced in a discrete mode into the electrolyte solution contained in the tube, which is placed close to the cell structure, one agent at the time being transported through the tube to and/or into said cell structure in which a pore has been formed by application of an electric field focused on the cell structure, resulting in electroporation of the cell structure. Also different applications of the method is disclosed, e.g. us of the method in order to transfer cell-impermeant solutes, such as drugs or genes, into the cell structure or out of the cell structure.
    Type: Application
    Filed: November 27, 2002
    Publication date: January 27, 2005
    Inventors: Owe Orwar, Mattias Karlsson, Kerstin Nolkrantz, Cecilia Farre
  • Publication number: 20040182707
    Abstract: The present invention relates to methods of measuring electrical properties of a cell using electrode devices comprising tapered nanotips having submicrometer dimensions (“nanoelectrodes”) for insertion into a cell. The devices are used to measure electrical properties of the cell and, optionally, may be used to electroporate, the cell or subcellular structures within the cell. The invention also provides arrays of electrode devices having nanotips for simultaneously or sequentially measuring the electrical properties of cells (e.g., such as surface immobilized cells). The electrodes can be used to measure properties of ion channels and in HTS assays to identify drugs which affect the properties of ion channels. The invention additionally provides microfluidic systems adapted for use with the electrode devices having nanotips.
    Type: Application
    Filed: October 15, 2003
    Publication date: September 23, 2004
    Applicant: Cellectricon AB
    Inventors: Kent Jardemark, Aldo Jesorka, Mattias Karlsson, Jessica Olofsson, Owe Orwar, Johan Pihl, Eskil Sahlin
  • Publication number: 20040112529
    Abstract: The invention provides integrated systems comprising macroscale devices interfaced with microscale devices and methods for making these systems.
    Type: Application
    Filed: October 8, 2003
    Publication date: June 17, 2004
    Applicant: CELLECTRICON AB
    Inventors: Mattias Karlsson, Owe Orwar, Daniel T. Chiu
  • Patent number: D598128
    Type: Grant
    Filed: March 12, 2008
    Date of Patent: August 11, 2009
    Assignee: Cellectricon AB
    Inventors: Johan Pihl, Mattias Karlsson