Patents by Inventor Meng H. Heng
Meng H. Heng has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Publication number: 20160264956Abstract: Methods are provided for recovery and formulation of insoluble enzymes from a microbial fermentation broth, without removal of microbial cells or cell debris. Granular and liquid formulations comprising insoluble enzymes are also provided.Type: ApplicationFiled: March 21, 2016Publication date: September 15, 2016Applicant: Danisco US Inc.Inventors: Michael Bodo, Robert I. Christensen, Rajdeep S. Dhaliwal, Meng H. Heng
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Patent number: 8993294Abstract: The present methods relate to the isolation and concentration of proteins using cross-flow membrane filtration, and to proteins produced by such methods. A feature of the method is that the protein of interest is retained by a cross-flow membrane under certain conditions that promote retention, while under other condition the protein passes through the membrane.Type: GrantFiled: August 10, 2010Date of Patent: March 31, 2015Assignee: Danisco US Inc.Inventors: Michael Bodo, Meng H. Heng
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Publication number: 20140166555Abstract: The invention relates to apparatuses, machines, systems and methods for the recovery and purification of proteins, peptides, nucleic acids, biologically produced polymers and other compounds from aqueous fluids. The aqueous fluids can comprise enzyme concentrates and or a fermentation broth with or without cells or other starting material. The fermentation broth can be produced by fermentations of fungal, yeast, bacterial, mammalian, insect or plant cells.Type: ApplicationFiled: August 8, 2013Publication date: June 19, 2014Applicant: Danisco US Inc.Inventors: Kevin R. DIBEL, Robin FONG, Meng H. HENG, Glenn ROZEBOOM
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Publication number: 20130337531Abstract: Methods are provided for recovery and formulation of insoluble enzymes from a microbial fermentation broth, without removal of microbial cells or cell debris. Granular and liquid formulations comprising insoluble enzymes are also provided.Type: ApplicationFiled: June 10, 2013Publication date: December 19, 2013Inventors: Michael BODO, Robert I. CHRISTENSEN, Rajdeep S. DHALIWAL, Meng H. HENG
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Publication number: 20120252066Abstract: The invention relates to a method for decreasing foam formation as well as maximizing expression of a biosurfactant in a microorganism. The methods encompasses precipitating a biosurfactant from the microorganism which results in decreased form formation.Type: ApplicationFiled: March 28, 2012Publication date: October 4, 2012Inventors: Meng H. Heng, Michael Bodo
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Publication number: 20120220009Abstract: The present methods relate to the isolation and concentration of proteins using cross-flow membrane filtration, and to proteins produced by such methods. A feature of the method is that the protein of interest is retained by a cross-flow membrane under certain conditions that promote retention, while under other condition the protein passes through the membrane.Type: ApplicationFiled: August 10, 2010Publication date: August 30, 2012Applicant: Danisco US Inc.Inventors: Michael Bodo, Meng H. Heng
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Publication number: 20110189344Abstract: Methods are provided for recovery and formulation of insoluble enzymes from a microbial fermentation broth, without removal of microbial cells or cell debris. Granular and liquid formulations comprising insoluble enzymes are also provided.Type: ApplicationFiled: June 9, 2009Publication date: August 4, 2011Inventors: Michael Bodo, Robert I. Christensen, Rajdeep S. Dhaliwal, Meng H. Heng
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Publication number: 20110008866Abstract: The invention relates to apparatuses, machines, systems and methods for the recovery and purification of proteins, peptides, nucleic acids, biologically produced polymers and other compounds from aqueous fluids. The aqueous fluids can comprise enzyme concentrates and or a fermentation broth with or without cells or other starting material. The fermentation broth can be produced by fermentations of fungal, yeast, bacterial, mammalian, insect or plant cells.Type: ApplicationFiled: September 17, 2010Publication date: January 13, 2011Inventors: Kevin R. Dibel, Robin Fong, Meng H. Heng, Glenn Rozeboom
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Patent number: 7850012Abstract: The present invention provides methods for separating one or more components of interest from a sample containing particulates and soluble materials. The method comprises the steps of: (a) filtering a sample through silica filter media whose surface silanol groups have reacted with one or more silanes, and (b) simultaneously capturing particulates and binding a soluble component to the silica filter media. The bound soluble component of interest is subsequently eluted from the silica filter media. In one embodiment of the invention, unwanted soluble materials are captured by the treated silica filter media and desired component of interest is recovered from the flow-through. In another embodiment, different components of interest are recovered from both the eluate and the flow-through. Preferred treated silica filter media are silane-treated rice hull ash or diatomaceous earth with functional quarternary ammonium group or functional sulphonate group.Type: GrantFiled: April 11, 2008Date of Patent: December 14, 2010Assignees: Dow Corning Corporation, Danisco US Inc.Inventors: Gary L. Gibson, Keith Hayes, Meng H. Heng, Csilla Kollar, Thomas H. Lane, Anthony Revis, Landon M. Steele
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Patent number: 7626004Abstract: The present invention relates to a method of purifying a protein of interest from its fusion analog, comprising obtaining a protein solution comprising a protein of interest and its fusion analog; adjusting the pH and/or ionic strength of the protein solution with an appropriate buffer for use with a Hydrophobic Charge Induction Chromatography (HCIC) resin, contacting the protein solution and HCIC resin column to allow binding of the protein of interest and its fusion analog, washing the resin and eluting the protein of interest from the resin by a pH gradient; wherein the protein of interest is substantially free of its fusion analog.Type: GrantFiled: March 13, 2007Date of Patent: December 1, 2009Assignee: Genencor International, Inc.Inventors: Robin Fong, Meng H. Heng
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Publication number: 20080185333Abstract: The present invention provides methods for separating one or more components of interest from a sample containing particulates and soluble materials. The method comprises the steps of: (a) filtering a sample through silica filter media whose surface silanol groups have reacted with one or more silanes, and (b) simultaneously capturing particulates and binding a soluble component to the silica filter media. The bound soluble component of interest is subsequently eluted from the silica filter media. In one embodiment of the invention, unwanted soluble materials are captured by the treated silica filter media and desired component of interest is recovered from the flow-through. In another embodiment, different components of interest are recovered from both the eluate and the flow-through. Preferred treated silica filter media are silane-treated rice hull ash or diatomaceous earth with functional quarternary ammonium group or functional sulphonate group.Type: ApplicationFiled: April 11, 2008Publication date: August 7, 2008Inventors: Gary L. Gibson, Keith Hayes, Meng H. Heng, Csilla Kollar, Thomas H. Lane, Anthony Revis, Landon M. Steele
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Patent number: 7374684Abstract: The present invention provides methods for separating one or more components of interest from a sample containing particulates and soluble materials. The method comprises the steps of: (a) filtering a sample through silica filter media whose surface silanol groups have reacted with one or more silanes, and (b) simultaneously capturing particulates and binding a soluble component to the silica filter media. The bound soluble component of interest is subsequently eluted from the silica filter media. In one embodiment of the invention, unwanted soluble materials are captured by the treated silica filter media and desired component of interest is recovered from the flow-through. In another embodiment, different components of interest are recovered from both the eluate and the flow-through. Preferred treated silica filter media are silane-treated rice hull ash or diatomaceous earth with functional quarternary ammonium group or functional sulphonate group.Type: GrantFiled: July 25, 2007Date of Patent: May 20, 2008Assignees: Dow Corning Corporation, Genencor International, Inc.Inventors: Gary L. Gibson, Keith Hayes, Meng H. Heng, Csilla Kollar, Thomas H. Lane, Anthony Revis, Landon M. Steele
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Patent number: 7264728Abstract: The present invention provides methods for separating one or more components of interest from a sample containing particulates and soluble materials. The method comprises the steps of: (a) filtering a sample through silica filter media whose surface silanol groups have reacted with one or more silanes, and (b) simultaneously capturing particulates and binding a soluble component to the silica filter media. The bound soluble component of interest is subsequently eluted from the silica filter media. In one embodiment of the invention, unwanted soluble materials are captured by the treated silica filter media and desired component of interest is recovered from the flow-through. In another embodiment of the invention, different components of interest are recovered from both the eluate and the flow-through. Preferred treated silica filter media are silane-treated rice hull ash or diatomaceous earth with functional quaternary ammonium group or functional sulphonate group.Type: GrantFiled: April 23, 2004Date of Patent: September 4, 2007Assignees: Dow Corning Corporation, Genencor International, Inc.Inventors: Gary L. Gibson, Keith Hayes, Meng H. Heng, Csilla Kollar, Thomas H. Lane, Anthony Revis, Landon M. Steele
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Patent number: 7208585Abstract: The present invention relates to a method of purifying a protein of interest from its fusion analog, comprising obtaining a protein solution comprising a protein of interest and its fusion analog; adjusting the pH and/or ionic strength of the protein solution with an appropriate buffer for use with a Hydrophobic Charge Induction Chromatograph (HCIC) resin, contacting the protein solution and HCIC resin column to allow binding of the protein of interest and its fusion analog, washing the resin and eluting the protein of interest from the resin by a pH gradient; wherein the protein of interest is substantially free of its fusion analog.Type: GrantFiled: September 15, 2003Date of Patent: April 24, 2007Assignee: Genencor International, Inc.Inventors: Robin Fong, Meng H. Heng
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Publication number: 20040211724Abstract: The present invention provides methods for separating one or more components of interest from a sample containing particulates and soluble materials. The method comprises the steps of: (a) filtering a sample through silica filter media whose surface silanol groups have reacted with one or more silanes, and (b) simultaneously capturing particulates and binding a soluble component to the silica filter media. The bound soluble component of interest is subsequently eluted from the silica filter media. In one embodiment of the invention, unwanted soluble materials are captured by the treated silica filter media and desired component of interest is recovered from the flow-through. In another embodiment of the invention, different components of interest are recovered from both the eluate and the flow-through. Preferred treated silica filter media are silane-treated rice hull ash or diatomaceous earth with functional quarternary ammonium group or functional sulphonate group.Type: ApplicationFiled: October 1, 2003Publication date: October 28, 2004Inventors: Gary L. Gibson, Keith Hayes, Meng H. Heng, Csilla Kollar, Thomas H. Lane, Anthony Revis, Landon M. Steele
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Publication number: 20040115785Abstract: Described herein are methods for purifying a protein of interest from a mixture of proteins wherein the mixture comprises the protein of interest and a fusion analog thereof. The method begins with a recovery in which filtration is used to remove cells and concentrate the protein of interest. Liquid chromatography is then used to remove fusion analogs and other contaminants resulting in a composition substantially free of contaminants. Substantially pure compositions of the protein of interest find use in therapeutic preparations.Type: ApplicationFiled: September 15, 2003Publication date: June 17, 2004Applicant: Genencor International, Inc.Inventors: Robin Fong, Meng H. Heng
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Patent number: 6593118Abstract: The present invention provides a crystallization process wherein a starting temperature is selected such that a desirable crystal morphology (e.g., square) is obtained. A temperature shift is then introduced, providing that the shift is not enough to induce further nucleation, where the crystals continue to grow in the desirable fashion, but with different kinetics, e.g., a higher rate of crystallization. As a result, the process gives a crystalline product with desirable morphology at a higher crystallization rate.Type: GrantFiled: November 2, 2001Date of Patent: July 15, 2003Assignee: Genencor International, Inc.Inventor: Meng H. Heng
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Publication number: 20020177206Abstract: The present invention provides a crystallization process wherein a starting temperature is selected such that a desirable crystal morphology (e.g., square) is obtained. A temperature shift is then introduced, providing that the shift is not enough to induce further nucleation, where the crystals continue to grow in the desirable fashion, but with different kinetics, e.g., a higher rate of crystallization. As a result, the process gives a crystalline product with desirable morphology at a higher crystallization rate.Type: ApplicationFiled: November 2, 2001Publication date: November 28, 2002Inventor: Meng H. Heng
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Patent number: 6403350Abstract: The present invention provides a crystallization process wherein a starting temperature is selected such that a desirable crystal morphology (e.g., square) is obtained. A temperature shift is then introduced, providing that the shift is not enough to induce further nucleation, where the crystals continue to grow in the desirable fashion, but with different kinetics, e.g., a higher rate of crystallization. As a result, the process gives a crystalline product with desirable morphology at a higher crystallization rate. The starting temperature of the process can be between about 4° C. and 20° C. for no more than about 5 hours and the temperature shift of the process can be between about 22° C. and 60° C. for no more than about 20 hours.Type: GrantFiled: March 3, 2000Date of Patent: June 11, 2002Assignee: Genencor International, Inc.Inventor: Meng H. Heng
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Patent number: 6190898Abstract: A method for preparing a crystalline cellulase enzyme is provided which comprises preparing an aqueous solution containing cellulase enzyme and adding to the aqueous solution a salt comprising an anion selected from the group consisting of sulfate, phosphate, formate, acetate, sorbate, chloride, bromide, fluoride or iodide, and a cation selected from the group consisting of sodium, ammonium, magnesium, potassium or calcium, wherein the aqueous solution is at a temperature between 10° C. and 60° C.Type: GrantFiled: March 1, 1996Date of Patent: February 20, 2001Assignee: Genencor International, Inc.Inventors: Nathaniel T. Becker, Edit L. Braunstein, Robert Fewkes, Ernst H. Gros, Meng H. Heng