Abstract: The invention belongs to the field of cosmetic biotechnology, and specifically relates to an Enterobacter that degrades hyaluronic acid and a cultivation method and application thereof. The Enterobacter sp. CGJ001 of the present invention was deposited in the China General Microbiological Culture Collection Center on Oct. 10, 2019, and the preservation number is CGMCC NO. 18661. The Enterobacter strain can efficiently produce hyaluronidase, and can be used in the process of preparing low molecular hyaluronic acid and oligomeric hyaluronic acid from high molecular hyaluronic acid. The enzyme has high specificity towards hyaluronic acid, excellent thermal stability and pH stability, and is suitable for large-scale industrial application. Thus, it can replace the traditional hyaluronidase extracted from expensive animal tissues. There should be broad application prospects in the fields of medicine and cosmetics.

Abstract: The present invention relates to a preparation method for acetylated sodium hyaluronate, which pertains to the field of organic chemical synthesis, characterized in that, the preparation method includes the following steps: in the protective atmosphere of inert gas, subjecting a hyaluronate and an acetic anhydride to a catalytic reaction in an organic solvent with a catalyst added, adding a water absorbent during the reaction, obtaining a reaction liquid, subjecting a reaction liquid to post treatment, separation, purification, and drying to obtain a product. A too high or too low degree of substitution is effectively avoided in the present invention, so as to obtain a product with a degree of substitution of acetyl groups ranging from 2.7 to 3.2, with mild reactive materials and reacting conditions, and simple separation and purification steps.

Abstract: The invention belongs to the field of cosmetic biotechnology, and specifically relates to an Enterobacter that degrades hyaluronic acid and a cultivation method and application thereof. The Enterobacter sp. CGJ001 of the present invention was deposited in the China General Microbiological Culture Collection Center on Oct. 10, 2019, and the preservation number is CGMCC NO. 18661. The Enterobacter strain can efficiently produce hyaluronidase, and can be used in the process of preparing low molecular hyaluronic acid and oligomeric hyaluronic acid from high molecular hyaluronic acid. The enzyme has high specificity towards hyaluronic acid, excellent thermal stability and pH stability, and is suitable for large-scale industrial application. Thus, it can replace the traditional hyaluronidase extracted from expensive animal tissues. There should be broad application prospects in the fields of medicine and cosmetics.

Abstract: The invention relates to an electrolyte arrangement for a cell having at least one anode (1) and at least one cathode (3) comprising at least three superposed layers (2.1, 2.2, 2.3), wherein the middle layer (2.2) comprises a porous electrically nonconductive structure, and wherein a layer of a polymer-based electrolyte (2.1, 2.3) is arranged on both opposite sides of the porous electrically nonconductive structure, wherein at least one of the superposed layers (2.1, 2.2, 2.3) contains a ceramic material, wherein the ceramic material of the middle layer (2.2) is selected from metal ion-conductive ceramic material, a ceramic material which does not conduct metal ions, and/or mixtures thereof, and the ceramic material of the polymer-based electrolyte layer(s) (2.1, 2.3) is a metal ion-conductive ceramic material.

Abstract: The invention belongs to the field of cosmetic biotechnology, and specifically relates to an Enterobacter that degrades hyaluronic acid and a cultivation method and application thereof. The Enterobacter sp. CGJ001 of the present invention was deposited in the China General Microbiological Culture Collection Center on Oct. 10, 2019, and the preservation number is CGMCC NO. 18661. The Enterobacter strain can efficiently produce hyaluronidase, and can be used in the process of preparing low molecular hyaluronic acid and oligomeric hyaluronic acid from high molecular hyaluronic acid. The enzyme has high specificity towards hyaluronic acid, excellent thermal stability and pH stability, and is suitable for large-scale industrial application. Thus it can replace the traditional hyaluronidase extracted from expensive animal tissues. There should be broad application prospects in the fields of medicine and cosmetics.