Patents by Inventor Michael Dolberg Rasmussen

Michael Dolberg Rasmussen has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Publication number: 20220298517
    Abstract: The present invention relates to a method for counter-selection by inhibition of conditionally essential genes.
    Type: Application
    Filed: June 16, 2020
    Publication date: September 22, 2022
    Applicant: Novozymes A/S
    Inventors: Steen Troels Joergensen, Michael Dolberg Rasmussen
  • Publication number: 20220275354
    Abstract: The present invention relates to temperature-sensitive variants of an RNA-guided endonuclease, polynucleotides encoding said variants, nucleic acid constructs and expression vectors comprising polynucleotides encoding said variants, host cells expressing said variant, methods of transient repression and expression of one or more DNA target sequence using said variants, and use of said variants, polynucleotides, nucleic acid constructs, expression vectors, host cells, and methods.
    Type: Application
    Filed: April 29, 2020
    Publication date: September 1, 2022
    Applicant: Novozymes A/S
    Inventors: Brian Koebmann, Michael Dolberg Rasmussen, Carsten Andersen
  • Publication number: 20220170003
    Abstract: The present invention relates to means and methods for improving protease expression by co-expression with a foldase.
    Type: Application
    Filed: March 4, 2020
    Publication date: June 2, 2022
    Applicant: Novozymes A/S
    Inventors: Ane Quesada Ganuza, Michael Dolberg Rasmussen, Allan Kent Nielsen
  • Publication number: 20220112478
    Abstract: The present invention relates to means and methods for optimizing expression of a heterologous polypeptide of interest in Gram-positive host cells by co-expression with a foldase that is cognate to the heterologous polypeptide of interest.
    Type: Application
    Filed: January 22, 2020
    Publication date: April 14, 2022
    Applicant: Novozymes A/S
    Inventors: Ane Quesada Ganuza, Michael Dolberg Rasmussen, Allan Kent Nielsen
  • Patent number: 11174487
    Abstract: Methods of constructing a cell comprising in its chromosome one or more copies of an open reading frame (ORF) or operon encoding at least one polypeptide of interest, each copy being under the transcriptional control of a heterologous promoter using a site specific recombinase and in vivo integration by recombination; resulting cells, and methods for producing a polypeptide of interest using the resulting cells.
    Type: Grant
    Filed: April 7, 2017
    Date of Patent: November 16, 2021
    Assignee: Novozymes A/S
    Inventors: Anne Breuner, Michael Dolberg Rasmussen
  • Patent number: 11130969
    Abstract: The present invention provides methods for directly transferring a recombinant polynucleotide of interest from the chromosome of a prokaryotic donor cell to the chromosome of a prokaryotic recipient cell of another species, wherein the polynucleotide of interest comprises at least one coding sequence of interest, preferably one gene of interest and a selectable marker, wherein said polynucleotide of interest in the chromosome of the donor cell is flanked on each side by a polynucleotide region derived from the chromosome of the recipient cell, and wherein the flanking polynucleotide regions are of sufficient size and sequence identity to allow double homologous recombination with the corresponding regions in the chromosome of the recipient cell.
    Type: Grant
    Filed: July 4, 2014
    Date of Patent: September 28, 2021
    Assignee: Novozymes, A/S
    Inventors: Michael Dolberg Rasmussen, Gitte Bak Poulsen
  • Publication number: 20210017544
    Abstract: The present invention relates to a method for counter-selection by inhibition conditionally essential genes.
    Type: Application
    Filed: December 12, 2008
    Publication date: January 21, 2021
    Applicant: Novozymes A/S
    Inventors: Michael Dolberg Rasmussen, Poul Erik Pedersen
  • Publication number: 20200318086
    Abstract: The present invention relates to temperature-sensitive variants of a Class-II Cas9 protein, polynucleotides encoding said variants, nucleic acid constructs and expression vectors comprising polynucleotides encoding said variants, host cells expressing said variant, methods of transient repression and expression of one or more genome target sequence using said variants, and use of said variants, polynucleotides, nucleic acid constructs, expression vectors, host cells, and methods.
    Type: Application
    Filed: November 7, 2018
    Publication date: October 8, 2020
    Applicant: Novozymes A/S
    Inventors: Carsten Anderson, Poul Erik Pedersen, Michael Dolberg Rasmussen, Carsten Hjort
  • Publication number: 20200181595
    Abstract: The present invention relates to Bacillus licheniformis host cells producing heterologous polypeptide of interest, wherein at least one gene in the lan gene cluster inactivated and methods for producing the polypeptide of interest by cultivating said cells.
    Type: Application
    Filed: May 12, 2016
    Publication date: June 11, 2020
    Applicant: Novozymes A/S
    Inventors: Brian Koebmann, Michael Dolberg Rasmussen
  • Publication number: 20190185847
    Abstract: The present invention relates to methods for improving at least one property of a microorganism host cell by repressing the expression of one or more genome target sequence of interest by CRISPR-inhibition as well as the resulting host cells and methods of production emplying said host cells.
    Type: Application
    Filed: July 5, 2017
    Publication date: June 20, 2019
    Applicant: Novozymes A/S
    Inventors: Michael Dolberg Rasmussen, Poul Erik Pedersen, Carsten Hjort, Barbara Cherry
  • Patent number: 10280426
    Abstract: A bacterial host cell is disclosed including at least two copies of an amplification unit in its genome, the amplification unit including: i) at least one copy of a gene of interest, and ii) an expressible conditionally essential gene, wherein the conditionally essential gene is either promoterless or transcribed from a heterologous promoter having an activity substantially lower than the endogenous promoter of the conditionally essential gene, and wherein the conditionally essential gene if not functional would render the cell auxotrophic for at least one specific substance or unable to utilize one or more specific sole carbon source; methods for producing a protein using the cell of the invention, and methods for constructing the cell of the invention.
    Type: Grant
    Filed: August 31, 2016
    Date of Patent: May 7, 2019
    Assignee: Novozymes A/S
    Inventors: Carsten Olsen, Michael Dolberg Rasmussen
  • Publication number: 20170218381
    Abstract: Methods of constructing a cell comprising in its chromosome one or more copies of an open reading frame (ORF) or operon encoding at least one polypeptide of interest, each copy being under the transcriptional control of a heterologous promoter using a site specific recombinase and in vivo integration by recombination; resulting cells, and methods for producing a polypeptide of interest using the resulting cells.
    Type: Application
    Filed: April 7, 2017
    Publication date: August 3, 2017
    Applicant: Novozymes A/S
    Inventors: Anne Breuner, Michael Dolberg Rasmussen
  • Publication number: 20160362698
    Abstract: A bacterial host cell is disclosed including at least two copies of an amplification unit in its genome, the amplification unit including: i) at least one copy of a gene of interest, and ii) an expressible conditionally essential gene, wherein the conditionally essential gene is either promoterless or transcribed from a heterologous promoter having an activity substantially lower than the endogenous promoter of the conditionally essential gene, and wherein the conditionally essential gene if not functional would render the cell auxotrophic for at least one specific substance or unable to utilize one or more specific sole carbon source; methods for producing a protein using the cell of the invention, and methods for constructing the cell of the invention.
    Type: Application
    Filed: August 31, 2016
    Publication date: December 15, 2016
    Applicant: Novozymes A/S
    Inventors: Carsten Olsen, Michael Dolberg Rasmussen
  • Patent number: 9458468
    Abstract: A bacterial host cell is disclosed including at least two copies of an amplification unit in its genome, the amplification unit including: i) at least one copy of a gene of interest, and ii) an expressible conditionally essential gene, wherein the conditionally essential gene is either promoterless or transcribed from a heterologous promoter having an activity substantially lower than the endogenous promoter of the conditionally essential gene, and wherein the conditionally essential gene if not functional would render the cell auxotrophic for at least one specific substance or unable to utilize one or more specific sole carbon source; methods for producing a protein using the cell of the invention, and methods for constructing the cell of the invention.
    Type: Grant
    Filed: February 14, 2014
    Date of Patent: October 4, 2016
    Assignee: Novozymes A/S
    Inventors: Carsten Olsen, Michael Dolberg Rasmussen
  • Publication number: 20160122772
    Abstract: The present invention relates to a method of producing a natively non-secreted polypeptide without a secretion signal in a Bacillus host cell and recovering the polypeptide without performing a lysis step as well as to a Bacillus host cell comprising one or more exogenous or heterologous polynucleotides encoding a natively non-secreted polypeptide with no secretion signal.
    Type: Application
    Filed: June 23, 2014
    Publication date: May 5, 2016
    Applicant: Novozymes A/S
    Inventors: Niels Banke, Michael Dolberg Rasmussen, Poul Erik Pedersen, Martin Simon Borchert
  • Publication number: 20160115501
    Abstract: The present invention provides methods for directly transferring a recombinant polynucleotide of interest from the chromosome of a prokaryotic donor cell to the chromosome of a prokaryotic recipient cell of another species, wherein the polynucleotide of interest comprises at least one coding sequence of interest, preferably one gene of interest and a selectable marker, wherein said polynucleotide of interest in the chromosome of the donor cell is flanked on each side by a polynucleotide region derived from the chromosome of the recipient cell, and wherein the flanking polynucleotide regions are of sufficient size and sequence identity to allow double homologous recombination with the corresponding regions in the chromosome of the recipient cell.
    Type: Application
    Filed: July 4, 2014
    Publication date: April 28, 2016
    Applicant: Novozymes A/S
    Inventors: Michael Dolberg Rasmussen, Gitte Bak Poulsen
  • Publication number: 20140273237
    Abstract: Methods of constructing a cell comprising in its chromosome one or more copies of an open reading frame (ORF) or operon encoding at least one polypeptide of interest, each copy being under the transcriptional control of a heterologous promoter using a site specific recombinase and in vivo integration by recombination; means for carrying out the methods, resulting cells, and methods for producing a polypeptide of interest using the resulting cells.
    Type: Application
    Filed: March 20, 2014
    Publication date: September 18, 2014
    Applicant: Novozymes A/S
    Inventors: Anne Breuner, Michael Dolberg Rasmussen
  • Publication number: 20140178930
    Abstract: A bacterial host cell is disclosed including at least two copies of an amplification unit in its genome, the amplification unit including: i) at least one copy of a gene of interest, and ii) an expressible conditionally essential gene, wherein the conditionally essential gene is either promoterless or transcribed from a heterologous promoter having an activity substantially lower than the endogenous promoter of the conditionally essential gene, and wherein the conditionally essential gene if not functional would render the cell auxotrophic for at least one specific substance or unable to utilize one or more specific sole carbon source; methods for producing a protein using the cell of the invention, and methods for constructing the cell of the invention.
    Type: Application
    Filed: February 14, 2014
    Publication date: June 26, 2014
    Inventors: Carsten Olsen, Michael Dolberg Rasmussen
  • Patent number: 8685653
    Abstract: A bacterial host cell is disclosed including at least two copies of an amplification unit in its genome, the amplification unit including: i) at least one copy of a gene of interest, and ii) an expressible conditionally essential gene, wherein the conditionally essential gene is either promoterless or transcribed from a heterologous promoter having an activity substantially lower than the endogenous promoter of the conditionally essential gene, and wherein the conditionally essential gene if not functional would render the cell auxotrophic for at least one specific substance or unable to utilize one or more specific sole carbon source; methods for producing a protein using the cell of the invention, and methods for constructing the cell of the invention.
    Type: Grant
    Filed: February 25, 2010
    Date of Patent: April 1, 2014
    Assignee: Novozymes A/S
    Inventors: Carsten Olsen, Michael Dolberg Rasmussen
  • Patent number: 8569029
    Abstract: The present invention relates to cells producing at least one polypeptide of interest and expressing one or more recombinant nuclease encoding gene(s) thereby producing the nuclease(s), and methods for producing a polypeptide of interest essentially free from contaminating DNA, said method comprising the steps of: (a) cultivating a cell that produces at least one polypeptide of interest and expresses one or more recombinant nuclease encoding gene(s) thereby producing the nuclease(s); and (b) isolating the polypeptide of interest.
    Type: Grant
    Filed: June 25, 2012
    Date of Patent: October 29, 2013
    Assignee: Novozymes A/S
    Inventors: Michael Dolberg Rasmussen, Jon Martin Persson