Patents by Inventor Michael Dolberg Rasmussen
Michael Dolberg Rasmussen has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Publication number: 20220298517Abstract: The present invention relates to a method for counter-selection by inhibition of conditionally essential genes.Type: ApplicationFiled: June 16, 2020Publication date: September 22, 2022Applicant: Novozymes A/SInventors: Steen Troels Joergensen, Michael Dolberg Rasmussen
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Publication number: 20220275354Abstract: The present invention relates to temperature-sensitive variants of an RNA-guided endonuclease, polynucleotides encoding said variants, nucleic acid constructs and expression vectors comprising polynucleotides encoding said variants, host cells expressing said variant, methods of transient repression and expression of one or more DNA target sequence using said variants, and use of said variants, polynucleotides, nucleic acid constructs, expression vectors, host cells, and methods.Type: ApplicationFiled: April 29, 2020Publication date: September 1, 2022Applicant: Novozymes A/SInventors: Brian Koebmann, Michael Dolberg Rasmussen, Carsten Andersen
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Publication number: 20220170003Abstract: The present invention relates to means and methods for improving protease expression by co-expression with a foldase.Type: ApplicationFiled: March 4, 2020Publication date: June 2, 2022Applicant: Novozymes A/SInventors: Ane Quesada Ganuza, Michael Dolberg Rasmussen, Allan Kent Nielsen
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Publication number: 20220112478Abstract: The present invention relates to means and methods for optimizing expression of a heterologous polypeptide of interest in Gram-positive host cells by co-expression with a foldase that is cognate to the heterologous polypeptide of interest.Type: ApplicationFiled: January 22, 2020Publication date: April 14, 2022Applicant: Novozymes A/SInventors: Ane Quesada Ganuza, Michael Dolberg Rasmussen, Allan Kent Nielsen
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Patent number: 11174487Abstract: Methods of constructing a cell comprising in its chromosome one or more copies of an open reading frame (ORF) or operon encoding at least one polypeptide of interest, each copy being under the transcriptional control of a heterologous promoter using a site specific recombinase and in vivo integration by recombination; resulting cells, and methods for producing a polypeptide of interest using the resulting cells.Type: GrantFiled: April 7, 2017Date of Patent: November 16, 2021Assignee: Novozymes A/SInventors: Anne Breuner, Michael Dolberg Rasmussen
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Patent number: 11130969Abstract: The present invention provides methods for directly transferring a recombinant polynucleotide of interest from the chromosome of a prokaryotic donor cell to the chromosome of a prokaryotic recipient cell of another species, wherein the polynucleotide of interest comprises at least one coding sequence of interest, preferably one gene of interest and a selectable marker, wherein said polynucleotide of interest in the chromosome of the donor cell is flanked on each side by a polynucleotide region derived from the chromosome of the recipient cell, and wherein the flanking polynucleotide regions are of sufficient size and sequence identity to allow double homologous recombination with the corresponding regions in the chromosome of the recipient cell.Type: GrantFiled: July 4, 2014Date of Patent: September 28, 2021Assignee: Novozymes, A/SInventors: Michael Dolberg Rasmussen, Gitte Bak Poulsen
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Publication number: 20210017544Abstract: The present invention relates to a method for counter-selection by inhibition conditionally essential genes.Type: ApplicationFiled: December 12, 2008Publication date: January 21, 2021Applicant: Novozymes A/SInventors: Michael Dolberg Rasmussen, Poul Erik Pedersen
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Publication number: 20200318086Abstract: The present invention relates to temperature-sensitive variants of a Class-II Cas9 protein, polynucleotides encoding said variants, nucleic acid constructs and expression vectors comprising polynucleotides encoding said variants, host cells expressing said variant, methods of transient repression and expression of one or more genome target sequence using said variants, and use of said variants, polynucleotides, nucleic acid constructs, expression vectors, host cells, and methods.Type: ApplicationFiled: November 7, 2018Publication date: October 8, 2020Applicant: Novozymes A/SInventors: Carsten Anderson, Poul Erik Pedersen, Michael Dolberg Rasmussen, Carsten Hjort
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Publication number: 20200181595Abstract: The present invention relates to Bacillus licheniformis host cells producing heterologous polypeptide of interest, wherein at least one gene in the lan gene cluster inactivated and methods for producing the polypeptide of interest by cultivating said cells.Type: ApplicationFiled: May 12, 2016Publication date: June 11, 2020Applicant: Novozymes A/SInventors: Brian Koebmann, Michael Dolberg Rasmussen
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Publication number: 20190185847Abstract: The present invention relates to methods for improving at least one property of a microorganism host cell by repressing the expression of one or more genome target sequence of interest by CRISPR-inhibition as well as the resulting host cells and methods of production emplying said host cells.Type: ApplicationFiled: July 5, 2017Publication date: June 20, 2019Applicant: Novozymes A/SInventors: Michael Dolberg Rasmussen, Poul Erik Pedersen, Carsten Hjort, Barbara Cherry
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Patent number: 10280426Abstract: A bacterial host cell is disclosed including at least two copies of an amplification unit in its genome, the amplification unit including: i) at least one copy of a gene of interest, and ii) an expressible conditionally essential gene, wherein the conditionally essential gene is either promoterless or transcribed from a heterologous promoter having an activity substantially lower than the endogenous promoter of the conditionally essential gene, and wherein the conditionally essential gene if not functional would render the cell auxotrophic for at least one specific substance or unable to utilize one or more specific sole carbon source; methods for producing a protein using the cell of the invention, and methods for constructing the cell of the invention.Type: GrantFiled: August 31, 2016Date of Patent: May 7, 2019Assignee: Novozymes A/SInventors: Carsten Olsen, Michael Dolberg Rasmussen
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Publication number: 20170218381Abstract: Methods of constructing a cell comprising in its chromosome one or more copies of an open reading frame (ORF) or operon encoding at least one polypeptide of interest, each copy being under the transcriptional control of a heterologous promoter using a site specific recombinase and in vivo integration by recombination; resulting cells, and methods for producing a polypeptide of interest using the resulting cells.Type: ApplicationFiled: April 7, 2017Publication date: August 3, 2017Applicant: Novozymes A/SInventors: Anne Breuner, Michael Dolberg Rasmussen
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Publication number: 20160362698Abstract: A bacterial host cell is disclosed including at least two copies of an amplification unit in its genome, the amplification unit including: i) at least one copy of a gene of interest, and ii) an expressible conditionally essential gene, wherein the conditionally essential gene is either promoterless or transcribed from a heterologous promoter having an activity substantially lower than the endogenous promoter of the conditionally essential gene, and wherein the conditionally essential gene if not functional would render the cell auxotrophic for at least one specific substance or unable to utilize one or more specific sole carbon source; methods for producing a protein using the cell of the invention, and methods for constructing the cell of the invention.Type: ApplicationFiled: August 31, 2016Publication date: December 15, 2016Applicant: Novozymes A/SInventors: Carsten Olsen, Michael Dolberg Rasmussen
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Patent number: 9458468Abstract: A bacterial host cell is disclosed including at least two copies of an amplification unit in its genome, the amplification unit including: i) at least one copy of a gene of interest, and ii) an expressible conditionally essential gene, wherein the conditionally essential gene is either promoterless or transcribed from a heterologous promoter having an activity substantially lower than the endogenous promoter of the conditionally essential gene, and wherein the conditionally essential gene if not functional would render the cell auxotrophic for at least one specific substance or unable to utilize one or more specific sole carbon source; methods for producing a protein using the cell of the invention, and methods for constructing the cell of the invention.Type: GrantFiled: February 14, 2014Date of Patent: October 4, 2016Assignee: Novozymes A/SInventors: Carsten Olsen, Michael Dolberg Rasmussen
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Publication number: 20160122772Abstract: The present invention relates to a method of producing a natively non-secreted polypeptide without a secretion signal in a Bacillus host cell and recovering the polypeptide without performing a lysis step as well as to a Bacillus host cell comprising one or more exogenous or heterologous polynucleotides encoding a natively non-secreted polypeptide with no secretion signal.Type: ApplicationFiled: June 23, 2014Publication date: May 5, 2016Applicant: Novozymes A/SInventors: Niels Banke, Michael Dolberg Rasmussen, Poul Erik Pedersen, Martin Simon Borchert
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Publication number: 20160115501Abstract: The present invention provides methods for directly transferring a recombinant polynucleotide of interest from the chromosome of a prokaryotic donor cell to the chromosome of a prokaryotic recipient cell of another species, wherein the polynucleotide of interest comprises at least one coding sequence of interest, preferably one gene of interest and a selectable marker, wherein said polynucleotide of interest in the chromosome of the donor cell is flanked on each side by a polynucleotide region derived from the chromosome of the recipient cell, and wherein the flanking polynucleotide regions are of sufficient size and sequence identity to allow double homologous recombination with the corresponding regions in the chromosome of the recipient cell.Type: ApplicationFiled: July 4, 2014Publication date: April 28, 2016Applicant: Novozymes A/SInventors: Michael Dolberg Rasmussen, Gitte Bak Poulsen
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Publication number: 20140273237Abstract: Methods of constructing a cell comprising in its chromosome one or more copies of an open reading frame (ORF) or operon encoding at least one polypeptide of interest, each copy being under the transcriptional control of a heterologous promoter using a site specific recombinase and in vivo integration by recombination; means for carrying out the methods, resulting cells, and methods for producing a polypeptide of interest using the resulting cells.Type: ApplicationFiled: March 20, 2014Publication date: September 18, 2014Applicant: Novozymes A/SInventors: Anne Breuner, Michael Dolberg Rasmussen
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Publication number: 20140178930Abstract: A bacterial host cell is disclosed including at least two copies of an amplification unit in its genome, the amplification unit including: i) at least one copy of a gene of interest, and ii) an expressible conditionally essential gene, wherein the conditionally essential gene is either promoterless or transcribed from a heterologous promoter having an activity substantially lower than the endogenous promoter of the conditionally essential gene, and wherein the conditionally essential gene if not functional would render the cell auxotrophic for at least one specific substance or unable to utilize one or more specific sole carbon source; methods for producing a protein using the cell of the invention, and methods for constructing the cell of the invention.Type: ApplicationFiled: February 14, 2014Publication date: June 26, 2014Inventors: Carsten Olsen, Michael Dolberg Rasmussen
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Patent number: 8685653Abstract: A bacterial host cell is disclosed including at least two copies of an amplification unit in its genome, the amplification unit including: i) at least one copy of a gene of interest, and ii) an expressible conditionally essential gene, wherein the conditionally essential gene is either promoterless or transcribed from a heterologous promoter having an activity substantially lower than the endogenous promoter of the conditionally essential gene, and wherein the conditionally essential gene if not functional would render the cell auxotrophic for at least one specific substance or unable to utilize one or more specific sole carbon source; methods for producing a protein using the cell of the invention, and methods for constructing the cell of the invention.Type: GrantFiled: February 25, 2010Date of Patent: April 1, 2014Assignee: Novozymes A/SInventors: Carsten Olsen, Michael Dolberg Rasmussen
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Patent number: 8569029Abstract: The present invention relates to cells producing at least one polypeptide of interest and expressing one or more recombinant nuclease encoding gene(s) thereby producing the nuclease(s), and methods for producing a polypeptide of interest essentially free from contaminating DNA, said method comprising the steps of: (a) cultivating a cell that produces at least one polypeptide of interest and expresses one or more recombinant nuclease encoding gene(s) thereby producing the nuclease(s); and (b) isolating the polypeptide of interest.Type: GrantFiled: June 25, 2012Date of Patent: October 29, 2013Assignee: Novozymes A/SInventors: Michael Dolberg Rasmussen, Jon Martin Persson