Patents by Inventor Michael Dorwart
Michael Dorwart has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Publication number: 20240409590Abstract: Described herein are variants of alpha-hemolysin having at least one mutation selected from T12R, T12K, N17R, N17K or combinations of T12 and N17 mutations. The variants in some embodiments may further comprise H144A. The ?-hemolysin variants have a decreased time to thread.Type: ApplicationFiled: June 18, 2024Publication date: December 12, 2024Applicant: Roche Sequencing Solutions, Inc.Inventors: Michael DORWART, Daniel KORENBLUM
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Patent number: 12043648Abstract: Described herein are variants of alpha-hemolysin having at least one mutation selected from T12R, T12K, N17R, N17K or combinations of T12 and N17 mutations. The variants in some embodiments may further comprise H144A. The ?-hemolysin variants have a decreased time to thread.Type: GrantFiled: September 21, 2022Date of Patent: July 23, 2024Assignee: Roche Sequencing Solutions, Inc.Inventors: Michael Dorwart, Daniel Korenblum
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Patent number: 11993807Abstract: An affinity reagent, having: (a) a retaining component such as a structured nucleic acid particle; and (b) one or both of (i) one or more label components attached to the retaining component, and (ii) one or more binding components attached to the retaining component.Type: GrantFiled: March 7, 2023Date of Patent: May 28, 2024Assignee: Nautilus Subsidiary, Inc.Inventors: Tural Aksel, Torri Rinker, Markus Burns, Michael Dorwart, Rachel Galimidi, Dmitriy Gremyachinskiy, Stephen Hendricks, Elvis Ikwa, Gregory Kapp, Joshua Simon Klein, Julia Robinson, Cassandra Stawicki, Sonal Tonapi, Parag Mallick
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Publication number: 20240018574Abstract: An affinity reagent, having: (a) a retaining component such as a structured nucleic acid particle; and (b) one or both of (i) one or more label components attached to the retaining component, and (ii) one or more binding components attached to the retaining component.Type: ApplicationFiled: June 2, 2023Publication date: January 18, 2024Inventors: Tural AKSEL, Torri RINKER, Markus BURNS, Michael DORWART, Rachel GALIMIDI, Dmitriy GREMYACHINSKIY, Stephen HENDRICKS, Elvis IKWA, Gregory KAPP, Joshua Simon KLEIN, Julia ROBINSON, Cassandra STAWICKI, Sonal TONAPI, Parag MALLICK
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Patent number: 11821034Abstract: Provided are methods of nucleic acid sample preparation for analysis, including analysis using nanopore sequencing applications. In particular provided are methods that allow for the creation of circular and linear DNA samples that contain asymmetric ends to ligate different and desired adaptors to the end of the sample, methods to create single nucleic acid molecules that automatically form concatemers for higher sequencing throughput; methods to create multiple copies (temperature dependent, multi-primed, open-fold replication) of one original nucleic acid sample, or to create single nucleic acid molecules containing multiple copies of more than one original nucleic acid sample (concatemerized samples) for improved accuracy and throughput are described; and methods for improved modified base calling.Type: GrantFiled: July 28, 2021Date of Patent: November 21, 2023Assignee: Roche Sequencing Solutions, Inc.Inventors: Randall Davis, Michael Dorwart, Janine Mok
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Publication number: 20230295247Abstract: Described herein are variants of alpha-hemolysin having at least one mutation selected from T12R, T12K, N17R, N17K or combinations of T12 and N17 mutations. The variants in some embodiments may further comprise H144A. The ?-hemolysin variants have a decreased time to thread.Type: ApplicationFiled: September 21, 2022Publication date: September 21, 2023Inventors: Michael DORWART, Daniel KORENBLUM
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Publication number: 20230272459Abstract: An affinity reagent, having: (a) a retaining component such as a structured nucleic acid particle; and (b) one or both of (i) one or more label components attached to the retaining component, and (ii) one or more binding components attached to the retaining component.Type: ApplicationFiled: March 7, 2023Publication date: August 31, 2023Inventors: Tural AKSEL, Torri RINKER, Markus BURNS, Michael DORWART, Rachel GALIMIDI, Dmitriy GREMYACHINSKIY, Stephen HENDRICKS, Elvis IKWA, Gregory KAPP, Joshua Simon KLEIN, Julia ROBINSON, Cassandra STAWICKI, Sonal TONAPI, Parag MALLICK
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Patent number: 11692217Abstract: An affinity reagent, having: (a) a retaining component such as a structured nucleic acid particle; and (b) one or both of (i) one or more label components attached to the retaining component, and (ii) one or more binding components attached to the retaining component.Type: GrantFiled: November 10, 2021Date of Patent: July 4, 2023Assignee: Nautilus Subsidiary, Inc.Inventors: Tural Aksel, Torri Rinker, Markus Burns, Michael Dorwart, Rachel Galimidi, Dmitriy Gremyachinskiy, Stephen Hendricks, Elvis Ikwa, Gregory Kapp, Joshua Simon Klein, Julia Robinson, Cassandra Stawicki, Sonal Tonapi, Parag Mallick
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Patent number: 11674174Abstract: This disclosure provides chips, systems and methods for sequencing a nucleic acid sample. Tagged nucleotides are provided into a reaction chamber comprising a nanopore in a membrane. An individual tagged nucleotide of the tagged nucleotides can contain a tag coupled to a nucleotide, which tag is detectable with the aid of the nanopore. Next, an individual tagged nucleotide of the tagged nucleotides can be incorporated into a growing strand complementary to a single stranded nucleic acid molecule derived from the nucleic acid sample. With the aid of the nanopore, a tag associated with the individual tagged nucleotide can be detected upon incorporation of the individual tagged nucleotide. The tag can be detected with the aid of the nanopore when the tag is released from the nucleotide.Type: GrantFiled: January 6, 2020Date of Patent: June 13, 2023Assignees: THE TRUSTEES OF COLUMBIA UNIVERSITY IN THE CITY OF NEW YORK, ROCHE SEQUENCING SOLUTIONS, INC.Inventors: Sergey Kalachikov, Jingyue Ju, Irina Morozova, Michael Dorwart
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Patent number: 11479584Abstract: Described herein are variants of alpha-hemolysin having at least one mutation selected from T12R, T12K, N17R, N17K or combinations of T12 and N17 mutations. The variants in some embodiments may further comprise H144A. The ?-hemolysin variants have a decreased time to thread.Type: GrantFiled: May 6, 2020Date of Patent: October 25, 2022Assignee: Roche Sequencing Solutions, Inc.Inventors: Michael Dorwart, Daniel Korenblum
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Publication number: 20220162684Abstract: An affinity reagent, having: (a) a retaining component such as a structured nucleic acid particle; and (b) one or both of (i) one or more label components attached to the retaining component, and (ii) one or more binding components attached to the retaining component.Type: ApplicationFiled: November 10, 2021Publication date: May 26, 2022Inventors: Tural AKSEL, Torri RINKER, Markus BURNS, Michael DORWART, Rachel GALIMIDI, Dmitriy GREMYACHINSKIY, Stephen HENDRICKS, Elvis IKWA, Gregory KAPP, Joshua KLEIN, Julia ROBINSON, Cassandra STAWICKI, Sonal TONAPI, Parag MALLICK
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Patent number: 11261488Abstract: Described herein are engineered alpha-hemolysin subunits having mutated oligomerization domains for assembling into heptameric nanopores in lipid bilayers.Type: GrantFiled: June 30, 2020Date of Patent: March 1, 2022Assignee: Roche Sequencing Solutions, Inc.Inventors: Timothy Kellogg Craig, Cynthia Ann Cech, Michael Dorwart, Liv Elisabeth Jensen, Marshall Winston Porter, Christos Tzitzilonis, Alexander Hyun-min Yang
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Publication number: 20220042968Abstract: Systems and methods for inserting a nanopore into a membrane covering a well are described herein. The membrane can be bowed outwards by establishing an osmotic gradient across the membrane in order to drive fluid into the well, which will increase the amount of fluid in the well and cause the membrane to bow outwards. Nanopore insertion can then be initiated on the bowed membrane.Type: ApplicationFiled: October 22, 2021Publication date: February 10, 2022Inventors: Geoffrey Barrall, Ashwini Bhat, Michael Dorwart, Jason Komadina, George Carman, Hannah Kallewaard-Lum, Kyle Umeda, Wooseok Jung, Yufang Wang
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Publication number: 20220017959Abstract: Provided are methods of nucleic acid sample preparation for analysis, including analysis using nanopore sequencing applications. In particular provided are methods that allow for the creation of circular and linear DNA samples that contain asymmetric ends to ligate different and desired adaptors to the end of the sample, methods to create single nucleic acid molecules that automatically form concatemers for higher sequencing throughput; methods to create multiple copies (temperature dependent, multi-primed, open-fold replication) of one original nucleic acid sample, or to create single nucleic acid molecules containing multiple copies of more than one original nucleic acid sample (concatemerized samples) for improved accuracy and throughput are described; and methods for improved modified base calling.Type: ApplicationFiled: July 28, 2021Publication date: January 20, 2022Inventors: Randall Davis, Michael Dorwart, Janine Mok
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Patent number: 11111534Abstract: Provided are methods of nucleic acid sample preparation for analysis, including analysis using nanopore sequencing applications. In particular provided are methods that allow for the creation of circular and linear DNA samples that contain asymmetric ends to ligate different and desired adaptors to the end of the sample, methods to create single nucleic acid molecules that automatically form concatemers for higher sequencing throughput; methods to create multiple copies (temperature dependent, multi-primed, open-fold replication) of one original nucleic acid sample, or to create single nucleic acid molecules containing multiple copies of more than one original nucleic acid sample (concatemerized samples) for improved accuracy and throughput are described; and methods for improved modified base calling.Type: GrantFiled: October 4, 2018Date of Patent: September 7, 2021Assignee: Roche Sequencing Solutions, inc.Inventors: Randall Davis, Michael Dorwart, Janine Mok
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Publication number: 20200339634Abstract: Described herein are variants of alpha-hemolysin having at least one mutation selected from T12R, T12K, N17R, N17K or combinations of T12 and N17 mutations. The variants in some embodiments may further comprise H144A. The ?-hemolysin variants have a decreased time to thread.Type: ApplicationFiled: May 6, 2020Publication date: October 29, 2020Applicant: Roche Sequencing Solutions, Inc.Inventors: Michael DORWART, Daniel KORENBLUM
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Publication number: 20200325534Abstract: Described herein are engineered alpha-hemolysin subunits having mutated oligomerization domains for assembling into heptameric nanopores in lipid bilayers.Type: ApplicationFiled: June 30, 2020Publication date: October 15, 2020Applicant: Roche Sequencing Solutions, Inc.Inventors: Timothy Kellogg CRAIG, Cynthia Ann CECH, Michael DORWART, Liv Elisabeth JENSEN, Marshall Winston PORTER, Christos TZITZILONIS, Alexander Hyun-min YANG
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Patent number: 10752948Abstract: Described herein are engineered alpha-hemolysin subunits having mutated oligomerization domains for assembling into heptameric nanopores in lipid bilayers.Type: GrantFiled: January 3, 2019Date of Patent: August 25, 2020Assignee: Roche Sequencing Solutions, Inc.Inventors: Timothy Kellogg Craig, Cynthia Ann Cech, Michael Dorwart, Liv Elisabeth Jensen, Marshall Winston Porter, Christos Tzitzilonis, Alexander Hyun-min Yang
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Publication number: 20200248250Abstract: This disclosure provides chips, systems and methods for sequencing a nucleic acid sample. Tagged nucleotides arc provided into a reaction chamber comprising a nanopore in a membrane. An individual tagged nucleotide of the tagged nucleotides can contain a tag coupled to a nucleotide, which tag is detectable with the aid of the nanopore. Next, an individual tagged nucleotide of the tagged nucleotides can be incorporated into a growing strand complementary to a single stranded nucleic acid molecule derived from the nucleic acid sample. With the aid of the nanopore, a tag associated with the individual tagged nucleotide can be detected upon incorporation of the individual tagged nucleotide. The tag can be detected with the aid of the nanopore when the tag is released from the nucleotide.Type: ApplicationFiled: January 6, 2020Publication date: August 6, 2020Applicants: The Trustees of Columbia University in the City of New York, Roche Sequencing Solutions, Inc.Inventors: Sergey Kalachikov, Jingyue Ju, Irina Morozova, Michael Dorwart
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Patent number: 10683331Abstract: Described herein are variants of alpha-hemolysin having at least one mutation selected from T12R, T12K, N17R, N17K or combinations of T12 and N17 mutations. The variants in some embodiments may further comprise H144A. The ?-hemolysin variants have a decreased time to thread.Type: GrantFiled: April 4, 2019Date of Patent: June 16, 2020Assignee: Roche Sequencing Solutions, Inc.Inventors: Michael Dorwart, Daniel Korenblum