Abstract: Provided herein is technology relating to the amplification-based detection of bisulfite-treated DNAs and particularly, but not exclusively, to methods and compositions for multiplex amplification of low-level sample DNA prior to further characterization of the sample DNA. The technology further provides methods for isolating DNA from blood or blood product samples, e.g., plasma samples.

Abstract: A method of treating a highly permeable subterranean formation that is penetrated by a wellbore to form a frac pack in the formation adjacent to a desired wellbore interval is provided. The method comprises (a) injecting a first high efficiency fracturing fluid into the formation to form a fracture in the formation that propagates from a near-wellbore region of the formation into a far-field region of the formation. Thereafter, high strength proppant is placed in a portion of the fracture in the near-wellbore region of the formation, and low strength proppant is placed in a portion of the fracture near the far-field region of the formation using low viscosity fluids. Subsequently, a high strength proppant is squeezed into a portion of the fracture in the near-wellbore region of the formation to assure that the fracture is completely packed.

Abstract: Well production enhancement systems and methods to enhance well production are disclosed. The method includes deploying an outer conveyance into a wellbore, where a plurality of propellants are deployed along a section of the outer conveyance. The method also includes deploying one or more isolation devices to form one or more isolation zones along the outer conveyance. The method further includes deploying an inner conveyance within the outer conveyance, where the inner conveyance is initially deployed along the section of the outer conveyance. The method further includes detonating the plurality of propellants to generate one or more fractures in a formation proximate to the section of the outer conveyance. The method further includes injecting fracture enhancement fluids into the one or more fractures to enhance well production through the one or more fractures.

Abstract: A method of treating a highly permeable subterranean formation that is penetrated by a wellbore to form a frac pack in the formation adjacent to a desired wellbore interval is provided. The method comprises (a) injecting a first high efficiency fracturing fluid into the formation to form a fracture in the formation that propagates from a near-wellbore region of the formation into a far-field region of the formation. Thereafter, high strength proppant is placed in a portion of the fracture in the near-wellbore region of the formation, and low strength proppant is placed in a portion of the fracture near the far-field region of the formation using low viscosity fluids. Subsequently, a high strength proppant is squeezed into a portion of the fracture in the near-wellbore region of the formation to assure that the fracture is completely packed.

Abstract: A downhole fluid composition having a saccharide gelling agent, an oxidative breaker, and a sequestering agent. The sequestering agent is hydrocarbon miscible and inert to oxidation by the oxidative breaker. The sequestering agent sequesters the oxidative breaker or an activator for the oxidative breaker, whereby oxidation of the gelling agent is inhibited. The downhole fluid may also include a proppant. Upon contacting hydrocarbons downhole or reaching a predetermined temperature, the sequestering agent releases the oxidative breaker or activator thereby oxidizing the saccharide gelling agent.

Abstract: Well production enhancement systems and methods to enhance well production are disclosed. The method includes deploying an outer conveyance into a wellbore, where a plurality of propellants are deployed along a section of the outer conveyance. The method also includes deploying one or more isolation devices to form one or more isolation zones along the outer conveyance. The method further includes deploying an inner conveyance within the outer conveyance, where the inner conveyance is initially deployed along the section of the outer conveyance. The method further includes detonating the plurality of propellants to generate one or more fractures in a formation proximate to the section of the outer conveyance. The method further includes injecting fracture enhancement fluids into the one or more fractures to enhance well production through the one or more fractures.

Abstract: Systems and methods for treating subterranean formations using proppants. More particularly, the systems and methods use sand or other particles having a wide particle-size distribution as a proppant for treating subterranean formations, including unconventional formations. In some embodiments, the methods comprise: providing a treatment fluid comprising a base fluid and unsieved proppant material; introducing the treatment fluid into a well bore penetrating at least a portion of a subterranean formation; and depositing at least a portion of the unsieved proppant material in at least a portion of the subterranean formation.

Abstract: Methods and systems for treating a fracture. An example method comprises providing a treatment fluid comprising a solubilized polyester and a water-miscible solvent; dispersing the treatment fluid into an aqueous fluid; wherein the dispersing the treatment fluid into an aqueous fluid precipitates the solubilized polyester and forms an amorphous polymeric structure comprising the solid polyester; introducing the amorphous polymeric structure into a fracture within a subterranean formation; and allowing the amorphous polymeric structure to degrade.

Abstract: A downhole fluid composition having a saccharide gelling agent, an oxidative breaker, and a sequestering agent. The sequestering agent is hydrocarbon miscible and inert to oxidation by the oxidative breaker. The sequestering agent sequesters the oxidative breaker or an activator for the oxidative breaker, whereby oxidation of the gelling agent is inhibited. The downhole fluid may also include a proppant. Upon contacting hydrocarbons downhole or reaching a predetermined temperature, the sequestering agent releases the oxidative breaker or activator thereby oxidizing the saccharide gelling agent.

Abstract: Provided are methods and systems for treating a fracture. An example method comprises providing a treatment fluid comprising a solubilized polyester and a water-miscible solvent; dispersing the treatment fluid into an aqueous fluid; wherein the dispersing the treatment fluid into an aqueous fluid precipitates the solubilized polyester and forms an amorphous polymeric structure comprising the solid polyester; introducing the amorphous polymeric structure into a fracture within a subterranean formation; and allowing the amorphous polymeric structure to degrade.

Abstract: Gravel pack fluids and methods of use thereof provides improve fluid suspension characteristics under extremely high temperatures, for example, from about 275° F. to 300° F. (135° C. to 149° C.) or higher. The disclosed the gravel pack fluids are able to achieve stability at these elevated temperatures by virtue of a gel composition having natural or synthetic hectorite nanoparticles added thereto. Such gravel pack fluids are especially useful for delivering proppant into a subterranean formation, gravel into a wellbore, and the like, in certain parts of the world known to have extremely high downhole temperatures. The disclosed gravel pack fluids are particularly useful for inclined and horizontal well applications, as well as applications that involve transporting particulates through alternate flow paths, such as shunt tubes, bypass conduits, and the like.

Abstract: A well screen can include multiple filter section ends, the filter section ends being displaced outward in response to expansion of the well screen, and a slurry delivery shunt conduit positioned circumferentially between the filter section ends. A method of delivering a slurry into a wellbore about a well screen can include positioning slurry delivery shunt conduits between multiple circumferentially spaced apart filter sections of the well screen, the filter sections radially outwardly displacing in a well, and flowing the slurry through the shunt conduits. A well system can include multiple well screens positioned in a wellbore, and shunt conduits which deliver a slurry into the wellbore about the well screens, the shunt conduits being positioned circumferentially between filter sections of the well screens.

Abstract: A method of fabricating a sand control screen assembly includes determining an anatomy of a facsimile sand control screen assembly, the sand control screen assembly including one or more component parts. A virtual three-dimensional (3D) model of the facsimile sand control screen assembly is then generated based on the anatomy. The virtual 3D model of the facsimile sand control screen assembly is provided to a 3D printer, and the 3D printer forms at least a portion of the facsimile sand control screen assembly based on the virtual 3D model.

Abstract: A method of treating a portion of a well comprising: forming a treatment fluid, wherein the treatment fluid comprises: (A) water; and (B) a surfactant, wherein the surfactant is environmentally-friendly, and wherein the surfactant causes a liquid hydrocarbon to become soluble in the treatment fluid; and introducing the treatment fluid into the well, wherein the treatment fluid comes in contact with wellbore particles after introduction into the well, wherein at least a portion of the wellbore particles are coated with the liquid hydrocarbon prior to contact with the treatment fluid.

Abstract: A method of fabricating a sand control screen assembly includes determining an anatomy of a facsimile sand control screen assembly, the sand control screen assembly including one or more component parts. A virtual three-dimensional (3D) model of the facsimile sand control screen assembly is then generated based on the anatomy. The virtual 3D model of the facsimile sand control screen assembly is provided to a 3D printer, and the 3D printer forms at least a portion of the facsimile sand control screen assembly based on the virtual 3D model.

Abstract: A well screen can include multiple filter section ends, the filter section ends being displaced outward in response to expansion of the well screen, and a slurry delivery shunt conduit positioned circumferentially between the filter section ends. A method of delivering a slurry into a wellbore about a well screen can include positioning slurry delivery shunt conduits between multiple circumferentially spaced apart filter sections of the well screen, the filter sections radially outwardly displacing in a well, and flowing the slurry through the shunt conduits. A well system can include multiple well screens positioned in a wellbore, and shunt conduits which deliver a slurry into the wellbore about the well screens, the shunt conduits being positioned circumferentially between filter sections of the well screens.

Abstract: Methods including providing at least one target interval in a wellbore, wherein the at least one target interval has a temperature of at least about 70° F. to at least about 290° F.; providing a pad fluid; providing a treatment fluid comprising proppant particulates coated with a one-step furan resin composition, wherein the one-step furan resin composition comprises a furan polymer, a hydrolyzable ester, a silane coupling agent, a surfactant, and a solvent; introducing the pad fluid into the wellbore at a rate and pressure sufficient to create or enhance at least one fracture within the target interval; introducing the treatment fluid into the wellbore so as to deposit the proppant particulates coated with the one-step furan resin composition into the at least one fracture; and setting the one-step furan resin composition so as to form at least a partially consolidated permeable proppant pack in the at least one fracture.

Abstract: Methods including providing at least one target interval in a wellbore, wherein the at least one target interval has a temperature of at least about 70° F. to at least about 290° F.; providing a pad fluid; providing a treatment fluid comprising proppant particulates coated with a one-step furan resin composition, wherein the one-step furan resin composition comprises a furan polymer, a hydrolyzable ester, a silane coupling agent, a surfactant, and a solvent; introducing the pad fluid into the wellbore at a rate and pressure sufficient to create or enhance at least one fracture within the target interval; introducing the treatment fluid into the wellbore so as to deposit the proppant particulates coated with the one-step furan resin composition into the at least one fracture; and setting the one-step furan resin composition so as to form at least a partially consolidated permeable proppant pack in the at least one fracture.

Abstract: Methods of treating a subterranean formation having at least one fracture including providing a cement slurry comprising an expandable cementitious material and a breakable foamed carrier fluid, wherein the expandable cementitious material is capable of consolidating to form a plurality of expandable cementitious material aggregates and wherein the breakable foamed carrier fluid is capable of coating and isolating the expandable cementitious material aggregates; introducing the cement slurry into the fracture; curing the expandable cementitious material aggregates so as to form a cement pillar within the fracture in the subterranean formation, wherein the curing of the expandable cementitious material aggregates expands the expandable cementitious material aggregates such that at least one microfracture is created within the fracture; breaking the breakable foamed carrier fluid; removing the broken breakable foamed carrier fluid from the subterranean formation; and acid-fracturing the at least one fracture in

Abstract: Methods of treating a wellbore in a subterranean formation comprising unconsolidated particulates including providing a treatment fluid comprising a furan-based resin and a stimuli-delayed acid generator; wherein the stimuli-delayed acid generator generates acid upon encountering a wellbore stimulus and wherein at least a portion of the stimuli-delayed acid generator is a liquid; introducing the treatment fluid into the wellbore in the subterranean formation; and stimulating the stimuli-delayed acid generator so as to generate acid and at least partially cure the furan-based resin; wherein the at least partial curing of the furan-based resin at least partially consolidates the unconsolidated particulates.